(Circular) (Six-base) MAPSORT of: pFN21AB5072.seq Check: 1297 from: 1 to: 6553 pFN21AB5072 6553 bp With 182 enzymes: SgfI * October 20, 2009 10:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4363 278 Size: 53 83 186 936 2827 2468 Fragments arranged by size: 2827 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2955 3896 1937 Size: 1018 941 4594 Fragments arranged by size: 4594 1018 941 Acc65I G'GTAC_C Cuts at: 3874 3874 Size: 6553 AclI AA'CG_TT Cuts at: 1563 4680 5053 1563 Size: 3117 373 3063 Fragments arranged by size: 3117 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2340 2810 3633 4616 6000 6268 1632 Size: 708 470 823 983 1384 268 1917 Fragments arranged by size: 1917 1384 983 823 708 470 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6356 AflIII A'CryG_T Cuts at: 1203 5452 6429 1203 Size: 4249 977 1327 Fragments arranged by size: 4249 1327 977 AgeI A'CCGG_T Cuts at: 5384 5384 Size: 6553 AhdI GACnn_n'nnGTC Cuts at: 5282 5282 Size: 6553 AleI CACnn'nnGTG Cuts at: 1166 3407 1166 Size: 2241 4312 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2366 1184 Size: 1182 5371 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2147 2179 2147 Size: 32 6521 AlwNI CAG_nnn'CTG Cuts at: 2226 4296 5868 2226 Size: 2070 1572 2911 Fragments arranged by size: 2911 2070 1572 ApaLI G'TGCA_C Cuts at: 4610 5766 4610 Size: 1156 5397 ApoI r'AATT_y Cuts at: 1493 3104 3521 3862 4088 4240 6349 1493 Size: 1611 417 341 226 152 2109 1697 Fragments arranged by size: 2109 1697 1611 417 341 226 152 AseI AT'TA_AT Cuts at: 160 1032 5107 160 Size: 872 4075 1606 Fragments arranged by size: 4075 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6553 AvaI C'yCGr_G Cuts at: 1870 1943 2369 2449 2649 2927 3147 3878 1870 Size: 73 426 80 200 278 220 731 4545 Fragments arranged by size: 4545 731 426 278 220 200 80 73 BamHI G'GATC_C Cuts at: 3883 4250 3883 Size: 367 6186 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2557 2783 3874 5329 618 Size: 325 217 627 770 226 1091 1455 1842 Fragments arranged by size: 1842 1455 1091 770 627 325 226 217 BanII G_rGCy'C Cuts at: 1391 3148 3872 1391 Size: 1757 724 4072 Fragments arranged by size: 4072 1757 724 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2991 3180 928 Size: 971 1092 189 4301 Fragments arranged by size: 4301 1092 971 189 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4744 4778 1903 Size: 34 2807 34 3678 Fragments arranged by size: 3678 2807 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2050 4445 5655 2050 Size: 2395 1210 2948 Fragments arranged by size: 2948 2395 1210 BclI T'GATC_A Cuts at: 1548 3478 1548 Size: 1930 4623 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2036 5164 136 Size: 107 122 71 631 605 364 3128 1525 Fragments arranged by size: 3128 1525 631 605 364 122 107 71 BglII A'GATC_T Cuts at: 6548 6548 Size: 6553 BlpI GC'TnA_GC Cuts at: 2018 3970 2018 Size: 1952 4601 Bme1580I G_kGCm'C Cuts at: 1790 2355 2562 4614 5770 1790 Size: 565 207 2052 1156 2573 Fragments arranged by size: 2573 2052 1156 565 207 BmgBI CAC'GTC Cuts at: 1318 3784 1318 Size: 2466 4087 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4284 5242 442 Size: 416 53 83 399 24 2867 958 1753 Fragments arranged by size: 2867 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6553 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2376 2659 5213 1239 Size: 1137 283 2554 2579 Fragments arranged by size: 2579 2554 1137 283 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4678 5543 5841 4678 Size: 865 298 5390 Fragments arranged by size: 5390 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3734 5216 882 Size: 617 2235 1482 2219 Fragments arranged by size: 2235 2219 1482 617 BsaAI yAC'GTr Cuts at: 493 1204 4344 493 Size: 711 3140 2702 Fragments arranged by size: 3140 2702 711 BsaBI GATnn'nnATC Cuts at: 6547 6547 Size: 6553 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2988 4360 4742 5377 Size: 53 83 186 936 1455 1372 382 635 Cuts at: 5377 275 Size: 1451 Fragments arranged by size: 1455 1451 1372 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4986 5384 5658 5805 6216 4986 Size: 398 274 147 411 5323 Fragments arranged by size: 5323 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3135 3165 1178 Size: 30 165 30 1732 30 4566 Fragments arranged by size: 4566 1732 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2379 1186 Size: 1193 5360 BseYI C'CCAG_C Cuts at: 2410 2430 2516 5756 6252 2410 Size: 20 86 3240 496 2711 Fragments arranged by size: 3240 2711 496 86 20 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5980 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4003 4764 5792 1540 Size: 275 186 2002 761 1028 2301 Fragments arranged by size: 2301 2002 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2608 4614 4699 5770 2608 Size: 2006 85 1071 3391 Fragments arranged by size: 3391 2006 1071 85 BsmI GAATG_Cn' Cuts at: 3700 4064 4157 3700 Size: 364 93 6096 Fragments arranged by size: 6096 364 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2355 2562 2608 3148 3872 4614 4699 Size: 399 565 207 46 540 724 742 85 Cuts at: 4699 5770 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 724 565 540 399 207 85 46 BspEI T'CCGG_A Cuts at: 6216 6216 Size: 6553 BspHI T'CATG_A Cuts at: 2139 3825 4442 6172 2139 Size: 1686 617 1730 2520 Fragments arranged by size: 2520 1730 1686 617 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2501 3908 844 Size: 1657 1407 3489 Fragments arranged by size: 3489 1657 1407 BsrBI CCG'CTC Cuts at: 1394 1823 3300 4440 1394 Size: 429 1477 1140 3507 Fragments arranged by size: 3507 1477 1140 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2031 2562 3201 5048 5222 65 Size: 1183 783 531 639 1847 174 1396 Fragments arranged by size: 1847 1396 1183 783 639 531 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5197 5384 1700 Size: 110 3387 187 2869 Fragments arranged by size: 3387 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6553 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6553 BssSI C'ACGA_G Cuts at: 2198 2607 4613 5625 2198 Size: 409 2006 1012 3126 Fragments arranged by size: 3126 2006 1012 409 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6553 BstBI TT'CG_AA Cuts at: 5357 5357 Size: 6553 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6553 BstXI CCAn_nnnn'nTGG Cuts at: 3778 3778 Size: 6553 BstYI r'GATC_y Cuts at: 1972 2628 2967 3883 4250 4635 4652 6104 6190 Size: 656 339 916 367 385 17 1452 86 Cuts at: 6190 6202 6548 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 916 656 385 367 346 339 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3792 513 Size: 552 939 1788 3274 Fragments arranged by size: 3274 1788 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5814 Fragments arranged by size: 5814 683 56 BtsI GCAGTG_nn' Cuts at: 4069 4863 4883 4069 Size: 794 20 5739 Fragments arranged by size: 5739 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6553 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6518 DraI TTT'AAA Cuts at: 3857 3857 Size: 6553 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5560 809 Size: 507 4244 1802 Fragments arranged by size: 4244 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2011 2037 3399 Size: 54 1425 50 275 18 181 26 1362 Cuts at: 3399 3811 4000 4889 6518 8 Size: 412 189 889 1629 43 Fragments arranged by size: 1629 1425 1362 889 412 275 189 181 54 50 43 26 18 EagI C'GGCC_G Cuts at: 1537 1812 4000 1537 Size: 275 2188 4090 Fragments arranged by size: 4090 2188 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4483 1353 Size: 3130 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2109 3318 5140 5514 5660 387 Size: 1064 138 520 1209 1822 374 146 1280 Fragments arranged by size: 1822 1280 1209 1064 520 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2340 2376 2659 2810 3633 4616 5213 Size: 393 708 36 283 151 823 983 597 Cuts at: 5213 6000 6268 1239 Size: 787 268 1524 Fragments arranged by size: 1524 983 823 787 708 597 393 283 268 151 36 EcoO109I rG'GnC_Cy Cuts at: 2427 2513 2427 Size: 86 6467 EcoRI G'AATT_C Cuts at: 3862 4240 3862 Size: 378 6175 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2681 2966 3638 2681 Size: 285 672 5596 Fragments arranged by size: 5596 672 285 FspI TGC'GCA Cuts at: 5059 5059 Size: 6553 HaeII r_GCGC'y Cuts at: 1678 2399 5700 1678 Size: 721 3301 2531 Fragments arranged by size: 3301 2531 721 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2271 2303 2329 2361 2921 Size: 32 135 32 699 32 26 32 560 Cuts at: 2921 2953 3135 3159 3167 3191 3335 3367 3714 Size: 32 182 24 8 24 144 32 347 Cuts at: 3714 3746 5194 5226 5268 5300 1373 Size: 32 1448 32 42 32 2626 Fragments arranged by size: 2626 1448 699 560 347 182 144 135 42 32 32 32 32 32 32 32 32 32 26 24 24 8 HincII GTy'rAC Cuts at: 669 1657 1938 2686 3897 4143 669 Size: 988 281 748 1211 246 3079 Fragments arranged by size: 3079 1211 988 748 281 246 HindIII A'AGCT_T Cuts at: 748 2663 3233 748 Size: 1915 570 4068 Fragments arranged by size: 4068 1915 570 HpaI GTT'AAC Cuts at: 4143 4143 Size: 6553 KpnI G_GTAC'C Cuts at: 3878 3878 Size: 6553 MfeI C'AATT_G Cuts at: 2213 4152 2213 Size: 1939 4614 MluI A'CGCG_T Cuts at: 6429 6429 Size: 6553 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2308 3289 5667 5851 1305 Size: 1003 981 2378 184 2007 Fragments arranged by size: 2378 2007 1003 981 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3401 10 Size: 54 1425 343 1569 3162 Fragments arranged by size: 3162 1569 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3407 3776 4511 4870 5029 518 Size: 648 2241 369 735 359 159 2042 Fragments arranged by size: 2241 2042 735 648 369 359 159 MspA1I CmG'CkG Cuts at: 1606 2017 2318 2816 3501 3969 4646 5794 6039 Size: 411 301 498 685 468 677 1148 245 Cuts at: 6039 1606 Size: 2120 Fragments arranged by size: 2120 1148 685 677 498 468 411 301 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5062 Fragments arranged by size: 5062 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6553 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6553 NotI GC'GGCC_GC Cuts at: 4000 4000 Size: 6553 NspI r_CATG'y Cuts at: 1132 1229 3436 3911 5456 1132 Size: 97 2207 475 1545 2229 Fragments arranged by size: 2229 2207 1545 475 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6553 PciI A'CATG_T Cuts at: 5452 5452 Size: 6553 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6553 PfoI T'CCnGG_A Cuts at: 3059 3600 3059 Size: 541 6012 PmeI GTTT'AAAC Cuts at: 3857 3857 Size: 6553 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4622 4654 6135 6167 4622 Size: 32 1481 32 5008 Fragments arranged by size: 5008 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2513 2513 Size: 6553 PshAI GACnn'nnGTC Cuts at: 1613 2858 1613 Size: 1245 5308 PsiI TTA'TAA Cuts at: 4123 4123 Size: 6553 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3419 3451 3419 Size: 32 6521 PstI C_TGCA'G Cuts at: 830 2512 3905 830 Size: 1682 1393 3478 Fragments arranged by size: 3478 1682 1393 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6553 PvuII CAG'CTG Cuts at: 2017 2318 2816 2017 Size: 301 498 5754 Fragments arranged by size: 5754 498 301 SalI G'TCGA_C Cuts at: 1936 3895 1936 Size: 1959 4594 SbfI CC_TGCA'GG Cuts at: 3905 3905 Size: 6553 ScaI AGT'ACT Cuts at: 4801 6513 4801 Size: 1712 4841 SfcI C'TryA_G Cuts at: 826 1045 2508 2709 3799 3901 5036 5717 5908 Size: 219 1463 201 1090 102 1135 681 191 Cuts at: 5908 826 Size: 1471 Fragments arranged by size: 1471 1463 1135 1090 681 219 201 191 102 SfiI GGCCn_nnn'nGGCC Cuts at: 2036 2036 Size: 6553 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6553 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6553 SmaI CCC'GGG Cuts at: 1872 3880 1872 Size: 2008 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2927 4657 5558 5820 820 Size: 197 926 984 1730 901 262 1553 Fragments arranged by size: 1730 1553 984 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4344 493 Size: 3851 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6553 SphI G_CATG'C Cuts at: 1132 3911 1132 Size: 2779 3774 SspI AAT'ATT Cuts at: 5 52 3562 4477 5 Size: 47 3510 915 2081 Fragments arranged by size: 3510 2081 915 47 StuI AGG'CCT Cuts at: 1517 3227 1517 Size: 1710 4843 StyI C'CwwG_G Cuts at: 513 1065 2004 3367 3622 3992 513 Size: 552 939 1363 255 370 3074 Fragments arranged by size: 3074 1363 939 552 370 255 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3577 4580 4597 4750 4935 5380 325 Size: 1255 1997 1003 17 153 185 445 1498 Fragments arranged by size: 1997 1498 1255 1003 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4799 6511 96 Size: 274 80 33 51 1443 2822 1712 138 Fragments arranged by size: 2822 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2587 2619 3508 3540 6513 6545 2587 Size: 32 889 32 2973 32 2595 Fragments arranged by size: 2973 2595 889 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6553 XbaI T'CTAG_A Cuts at: 3116 3889 3116 Size: 773 5780 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2438 2438 Size: 6553 XhoI C'TCGA_G Cuts at: 1943 2927 1943 Size: 984 5569 XmaI C'CCGG_G Cuts at: 1870 3878 1870 Size: 2008 4545 XmnI GAAnn'nnTTC Cuts at: 2458 2666 4682 2458 Size: 208 2016 4329 Fragments arranged by size: 4329 2016 208 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4361 276 Size: 53 83 186 936 2827 2468 Fragments arranged by size: 2827 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfoI SrfI SwaI