(Circular) (Six-base) MAPSORT of: pFN21AB5029.seq Check: 7806 from: 1 to: 6349 pFN21AB5029 6349 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3078 3078 Size: 6349 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4159 278 Size: 53 83 186 936 2623 2468 Fragments arranged by size: 2623 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3561 3692 1937 Size: 1624 131 4594 Fragments arranged by size: 4594 1624 131 Acc65I G'GTAC_C Cuts at: 3366 3670 3366 Size: 304 6045 AclI AA'CG_TT Cuts at: 1563 4476 4849 1563 Size: 2913 373 3063 Fragments arranged by size: 3063 2913 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2469 3299 3568 4412 5796 6064 1632 Size: 837 830 269 844 1384 268 1917 Fragments arranged by size: 1917 1384 844 837 830 269 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6152 AflIII A'CryG_T Cuts at: 1203 2343 5248 6225 1203 Size: 1140 2905 977 1327 Fragments arranged by size: 2905 1327 1140 977 AgeI A'CCGG_T Cuts at: 5180 5180 Size: 6349 AhdI GACnn_n'nnGTC Cuts at: 5078 5078 Size: 6349 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6349 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2426 1184 Size: 1242 5107 AlwNI CAG_nnn'CTG Cuts at: 2368 3213 3241 4092 5664 2368 Size: 845 28 851 1572 3053 Fragments arranged by size: 3053 1572 851 845 28 ApaI G_GGCC'C Cuts at: 2599 2599 Size: 6349 ApaLI G'TGCA_C Cuts at: 4406 5562 4406 Size: 1156 5193 ApoI r'AATT_y Cuts at: 1493 3658 3884 4036 6145 1493 Size: 2165 226 152 2109 1697 Fragments arranged by size: 2165 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4903 160 Size: 872 3871 1606 Fragments arranged by size: 3871 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6349 AvaI C'yCGr_G Cuts at: 1870 1943 2069 2475 3674 1870 Size: 73 126 406 1199 4545 Fragments arranged by size: 4545 1199 406 126 73 AvrII C'CTAG_G Cuts at: 3400 3400 Size: 6349 BamHI G'GATC_C Cuts at: 3679 4046 3679 Size: 367 5982 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3366 3404 3578 3670 5125 Size: 325 217 627 1579 38 174 92 1455 Cuts at: 5125 618 Size: 1842 Fragments arranged by size: 1842 1579 1455 627 325 217 174 92 38 BanII G_rGCy'C Cuts at: 1391 2599 2898 3668 1391 Size: 1208 299 770 4072 Fragments arranged by size: 4072 1208 770 299 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2279 928 Size: 971 380 4998 Fragments arranged by size: 4998 971 380 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4540 4574 1903 Size: 34 2603 34 3678 Fragments arranged by size: 3678 2603 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2248 4241 5451 2248 Size: 1993 1210 3146 Fragments arranged by size: 3146 1993 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6349 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2759 2948 4960 Size: 107 122 71 631 605 1087 189 2012 Cuts at: 4960 136 Size: 1525 Fragments arranged by size: 2012 1525 1087 631 605 189 122 107 71 BglII A'GATC_T Cuts at: 6344 6344 Size: 6349 BlpI GC'TnA_GC Cuts at: 3766 3766 Size: 6349 Bme1580I G_kGCm'C Cuts at: 1790 2599 4410 5566 1790 Size: 809 1811 1156 2573 Fragments arranged by size: 2573 1811 1156 809 BmgBI CAC'GTC Cuts at: 1318 3394 1318 Size: 2076 4273 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4080 5038 442 Size: 416 53 83 399 24 2663 958 1753 Fragments arranged by size: 2663 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6349 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3171 3527 5009 1239 Size: 1932 356 1482 2579 Fragments arranged by size: 2579 1932 1482 356 Bpu10I CC'TnA_GC Cuts at: 3449 3449 Size: 6349 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2886 3007 3329 3515 4474 5339 5637 2886 Size: 121 322 186 959 865 298 3598 Fragments arranged by size: 3598 959 865 322 298 186 121 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5012 882 Size: 617 3513 2219 Fragments arranged by size: 3513 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3373 4140 493 Size: 711 2169 767 2702 Fragments arranged by size: 2702 2169 767 711 BsaBI GATnn'nnATC Cuts at: 6343 6343 Size: 6349 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3141 3232 4156 4538 Size: 53 83 186 936 1608 91 924 382 Cuts at: 4538 5173 275 Size: 635 1451 Fragments arranged by size: 1608 1451 936 924 635 382 186 91 83 53 BsaWI w'CCGG_w Cuts at: 4782 5180 5454 5601 6012 4782 Size: 398 274 147 411 5119 Fragments arranged by size: 5119 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2957 2987 1178 Size: 30 165 30 1554 30 4540 Fragments arranged by size: 4540 1554 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2173 2240 2653 3373 3524 3617 3626 3629 Size: 987 67 413 720 151 93 9 3 Cuts at: 3629 1186 Size: 3906 Fragments arranged by size: 3906 987 720 413 151 93 67 9 3 BseYI C'CCAG_C Cuts at: 2049 2976 3408 5552 6048 2049 Size: 927 432 2144 496 2350 Fragments arranged by size: 2350 2144 927 496 432 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5776 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3799 4560 5588 1540 Size: 275 186 1798 761 1028 2301 Fragments arranged by size: 2301 1798 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3135 4410 4495 5566 3135 Size: 1275 85 1071 3918 Fragments arranged by size: 3918 1275 1071 85 BsmI GAATG_Cn' Cuts at: 2403 3466 3860 3953 2403 Size: 1063 394 93 4799 Fragments arranged by size: 4799 1063 394 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2599 2898 3135 3668 4410 4495 5566 Size: 399 809 299 237 533 742 85 1071 Cuts at: 5566 1391 Size: 2174 Fragments arranged by size: 2174 1071 809 742 533 399 299 237 85 BspEI T'CCGG_A Cuts at: 6012 6012 Size: 6349 BspHI T'CATG_A Cuts at: 2016 3144 4238 5968 2016 Size: 1128 1094 1730 2397 Fragments arranged by size: 2397 1730 1128 1094 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2367 3078 3320 3538 3704 844 Size: 1523 711 242 218 166 3489 Fragments arranged by size: 3489 1523 711 242 218 166 BsrBI CCG'CTC Cuts at: 1394 1823 2784 4236 1394 Size: 429 961 1452 3507 Fragments arranged by size: 3507 1452 961 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3264 4844 5018 65 Size: 1183 2016 1580 174 1396 Fragments arranged by size: 2016 1580 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4993 5180 1700 Size: 110 3183 187 2869 Fragments arranged by size: 3183 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6349 BssHII G'CGCG_C Cuts at: 1925 2261 1925 Size: 336 6013 BssSI C'ACGA_G Cuts at: 2635 4409 5421 2635 Size: 1774 1012 3563 Fragments arranged by size: 3563 1774 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2368 794 Size: 1574 4775 BstBI TT'CG_AA Cuts at: 5153 5153 Size: 6349 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6349 BstXI CCAn_nnnn'nTGG Cuts at: 2819 2819 Size: 6349 BstYI r'GATC_y Cuts at: 1972 2364 3332 3679 4046 4431 4448 5900 5986 Size: 392 968 347 367 385 17 1452 86 Cuts at: 5986 5998 6344 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 968 392 385 367 347 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2044 2044 Size: 6349 BtgI C'CryG_G Cuts at: 513 1065 2004 2943 3270 513 Size: 552 939 939 327 3592 Fragments arranged by size: 3592 939 939 552 327 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5610 Fragments arranged by size: 5610 683 56 BtsI GCAGTG_nn' Cuts at: 2651 2987 3865 4659 4679 2651 Size: 336 878 794 20 4321 Fragments arranged by size: 4321 878 794 336 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6349 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6314 DraI TTT'AAA Cuts at: 3653 3653 Size: 6349 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2702 3119 5356 809 Size: 507 1386 417 2237 1802 Fragments arranged by size: 2237 1802 1386 507 417 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2760 2940 3796 Size: 54 1425 50 275 18 930 180 856 Cuts at: 3796 4685 6314 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 930 889 856 275 180 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3796 1537 Size: 275 1984 4090 Fragments arranged by size: 4090 1984 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2488 2837 3603 3618 3630 4279 1353 Size: 1135 349 766 15 12 649 3423 Fragments arranged by size: 3423 1135 766 649 349 15 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4936 5310 5456 387 Size: 1064 138 3347 374 146 1280 Fragments arranged by size: 3347 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2469 3171 3299 3527 3568 4412 5009 Size: 393 837 702 128 228 41 844 597 Cuts at: 5009 5796 6064 1239 Size: 787 268 1524 Fragments arranged by size: 1524 844 837 787 702 597 393 268 228 128 41 EcoO109I rG'GnC_Cy Cuts at: 2054 2595 2596 3535 2054 Size: 541 1 939 4868 Fragments arranged by size: 4868 939 541 1 EcoRI G'AATT_C Cuts at: 3658 4036 3658 Size: 378 5971 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2689 2689 Size: 6349 FspI TGC'GCA Cuts at: 4855 4855 Size: 6349 HaeII r_GCGC'y Cuts at: 1678 5496 1678 Size: 3818 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2027 2059 2447 2479 3246 Size: 32 135 32 455 32 388 32 767 Cuts at: 3246 3278 4990 5022 5064 5096 1373 Size: 32 1712 32 42 32 2626 Fragments arranged by size: 2626 1712 767 455 388 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3226 3693 3939 669 Size: 988 281 1288 467 246 3079 Fragments arranged by size: 3079 1288 988 467 281 246 HindIII A'AGCT_T Cuts at: 748 3095 3347 748 Size: 2347 252 3750 Fragments arranged by size: 3750 2347 252 HpaI GTT'AAC Cuts at: 3939 3939 Size: 6349 KpnI G_GTAC'C Cuts at: 3370 3674 3370 Size: 304 6045 MfeI C'AATT_G Cuts at: 3948 3948 Size: 6349 MluI A'CGCG_T Cuts at: 6225 6225 Size: 6349 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2318 5463 5647 1305 Size: 1013 3145 184 2007 Fragments arranged by size: 3145 2007 1013 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2762 2942 10 Size: 54 1425 343 930 180 3417 Fragments arranged by size: 3417 1425 930 343 180 54 MslI CAynn'nnrTG Cuts at: 518 1166 2768 3216 3497 4307 4666 4825 518 Size: 648 1602 448 281 810 359 159 2042 Fragments arranged by size: 2042 1602 810 648 448 359 281 159 MspA1I CmG'CkG Cuts at: 1606 2248 2287 3765 4442 5590 5835 1606 Size: 642 39 1478 677 1148 245 2120 Fragments arranged by size: 2120 1478 1148 677 642 245 39 NcoI C'CATG_G Cuts at: 513 1065 2004 2943 513 Size: 552 939 939 3919 Fragments arranged by size: 3919 939 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6349 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6349 NotI GC'GGCC_GC Cuts at: 3796 3796 Size: 6349 NspI r_CATG'y Cuts at: 1132 1229 2347 3707 5252 1132 Size: 97 1118 1360 1545 2229 Fragments arranged by size: 2229 1545 1360 1118 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6349 PciI A'CATG_T Cuts at: 2343 5248 2343 Size: 2905 3444 PflMI CCAn_nnn'nTGG Cuts at: 1108 2251 1108 Size: 1143 5206 PfoI T'CCnGG_A Cuts at: 2031 2031 Size: 6349 PmeI GTTT'AAAC Cuts at: 3653 3653 Size: 6349 PmlI CAC'GTG Cuts at: 3373 3373 Size: 6349 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4418 4450 5931 5963 4418 Size: 32 1481 32 4804 Fragments arranged by size: 4804 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2054 3535 2054 Size: 1481 4868 PshAI GACnn'nnGTC Cuts at: 1613 3231 1613 Size: 1618 4731 PsiI TTA'TAA Cuts at: 3919 3919 Size: 6349 PspOMI G'GGCC_C Cuts at: 2595 2595 Size: 6349 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3275 3307 3275 Size: 32 6317 PstI C_TGCA'G Cuts at: 830 2364 2415 3701 830 Size: 1534 51 1286 3478 Fragments arranged by size: 3478 1534 1286 51 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6349 PvuII CAG'CTG Cuts at: 2248 2248 Size: 6349 SalI G'TCGA_C Cuts at: 1936 3691 1936 Size: 1755 4594 SanDI GG'GwC_CC Cuts at: 2054 2054 Size: 6349 SbfI CC_TGCA'GG Cuts at: 3701 3701 Size: 6349 ScaI AGT'ACT Cuts at: 2180 4597 6309 2180 Size: 2417 1712 2220 Fragments arranged by size: 2417 2220 1712 SfcI C'TryA_G Cuts at: 826 1045 2360 2411 3562 3697 4832 5513 5704 Size: 219 1315 51 1151 135 1135 681 191 Cuts at: 5704 826 Size: 1471 Fragments arranged by size: 1471 1315 1151 1135 681 219 191 135 51 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6349 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6349 SmaI CCC'GGG Cuts at: 1872 3676 1872 Size: 1804 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2901 2986 3344 3530 4453 5354 Size: 197 926 958 85 358 186 923 901 Cuts at: 5354 5616 820 Size: 262 1553 Fragments arranged by size: 1553 958 926 923 901 358 262 197 186 85 SnaBI TAC'GTA Cuts at: 493 4140 493 Size: 3647 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6349 SphI G_CATG'C Cuts at: 1132 3707 1132 Size: 2575 3774 SspI AAT'ATT Cuts at: 5 52 4273 5 Size: 47 4221 2081 Fragments arranged by size: 4221 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6349 StyI C'CwwG_G Cuts at: 513 1065 2004 2943 3400 3788 513 Size: 552 939 939 457 388 3074 Fragments arranged by size: 3074 939 939 552 457 388 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3422 4376 4393 4546 4731 5176 325 Size: 1255 1842 954 17 153 185 445 1498 Fragments arranged by size: 1842 1498 1255 954 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2178 4595 6307 Size: 274 80 33 51 1443 201 2417 1712 Cuts at: 6307 96 Size: 138 Fragments arranged by size: 2417 1712 1443 274 201 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2013 2045 2570 2602 2957 2989 3301 3333 6309 Size: 32 525 32 355 32 312 32 2976 Cuts at: 6309 6341 2013 Size: 32 2021 Fragments arranged by size: 2976 2021 525 355 312 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2702 1316 Size: 1386 4963 XbaI T'CTAG_A Cuts at: 3685 3685 Size: 6349 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2432 2432 Size: 6349 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6349 XmaI C'CCGG_G Cuts at: 1870 3674 1870 Size: 1804 4545 XmnI GAAnn'nnTTC Cuts at: 2403 2420 4478 2403 Size: 17 2058 4274 Fragments arranged by size: 4274 2058 17 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4157 276 Size: 53 83 186 936 2623 2468 Fragments arranged by size: 2623 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI