Restriction map for pFN21AB5014
 (Linear) (Six-base) MAPSORT of: KIBB5014.seq  Check: 5483  from: 1  to: 1251



KIBB5014.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:26  ..

AccI GT'mk_AC

Cuts at:      0      62    1251 
   Size:         62    1189
 
AflII C'TTAA_G

Cuts at:      0    1099    1251 
   Size:       1099     152
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     478    1251 
   Size:        478     773
 
AlwNI CAG_nnn'CTG

Cuts at:      0      81     270    1251 
   Size:         81     189     981

  Fragments arranged by size: 

                 981     189      81
 
ApoI r'AATT_y

Cuts at:      0     142    1251 
   Size:        142    1109
 
AvaI C'yCGr_G

Cuts at:      0     715     748     889    1251 
   Size:        715      33     141     362

  Fragments arranged by size: 

                 715     362     141      33
 
BamHI G'GATC_C

Cuts at:      0     627     866    1251 
   Size:        627     239     385

  Fragments arranged by size: 

                 627     385     239
 
BanI G'GyrC_C

Cuts at:      0     418     970    1251 
   Size:        418     552     281

  Fragments arranged by size: 

                 552     418     281
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     596     656    1121    1251 
   Size:        596      60     465     130

  Fragments arranged by size: 

                 596     465     130      60
 
BbvCI CC'TCA_GC

Cuts at:      0    1016    1251 
   Size:       1016     235
 
BclI T'GATC_A

Cuts at:      0     467    1251 
   Size:        467     784
 
BglI GCCn_nnn'nGGC

Cuts at:      0      35    1251 
   Size:         35    1216
 
Bme1580I G_kGCm'C

Cuts at:      0     862    1251 
   Size:        862     389
 
BmgBI CAC'GTC

Cuts at:      0    1037    1251 
   Size:       1037     214
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     663     714     765     784     902    1251 
   Size:        663      51      51      19     118     349

  Fragments arranged by size: 

                 663     349     118      51      51      19
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     597    1251 
   Size:        597     654
 
Bpu10I CC'TnA_GC

Cuts at:      0    1016    1251 
   Size:       1016     235
 
BsaWI w'CCGG_w

Cuts at:      0     645    1251 
   Size:        645     606
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      22     193    1033    1102    1120    1147    1150    1153 
   Size:         22     171     840      69      18      27       3       3

Cuts at:   1153    1198    1207    1228    1251 
   Size:         45       9      21      23

  Fragments arranged by size: 

                 840     171      69      45      27      23      22      21
                  18       9       3       3
 
BseYI C'CCAG_C

Cuts at:      0     939    1251 
   Size:        939     312
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     298    1251 
   Size:        298     953
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      83    1027    1251 
   Size:         83     944     224

  Fragments arranged by size: 

                 944     224      83
 
Bsp1286I G_dGCh'C

Cuts at:      0     862    1251 
   Size:        862     389
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     948    1251 
   Size:        948     303
 
BsrBI CCG'CTC

Cuts at:      0     663    1251 
   Size:        663     588
 
BsrGI T'GTAC_A

Cuts at:      0     512    1251 
   Size:        512     739
 
BstYI r'GATC_y

Cuts at:      0     627     866    1251 
   Size:        627     239     385

  Fragments arranged by size: 

                 627     385     239
 
Bsu36I CC'TnA_GG

Cuts at:      0     698    1251 
   Size:        698     553
 
ClaI AT'CG_AT

Cuts at:      0     674    1251 
   Size:        674     577
 
EaeI y'GGCC_r

Cuts at:      0      41     233     529    1230    1251 
   Size:         41     192     296     701      21

  Fragments arranged by size: 

                 701     296     192      41      21
 
EarI CTCTTCn'nnn_

Cuts at:      0     758    1251 
   Size:        758     493
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     955    1251 
   Size:        955     296
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     612     644     663     695    1251 
   Size:        612      32      19      32     556

  Fragments arranged by size: 

                 612     556      32      32      19
 
MspA1I CmG'CkG

Cuts at:      0      18     107    1251 
   Size:         18      89    1144

  Fragments arranged by size: 

                1144      89      18
 
NspI r_CATG'y

Cuts at:      0     366    1251 
   Size:        366     885
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     270    1251 
   Size:        270     981
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     793     825    1251 
   Size:        793      32     426

  Fragments arranged by size: 

                 793     426      32
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      35    1251 
   Size:         35    1216
 
SmlI C'TyrA_G

Cuts at:      0    1099    1251 
   Size:       1099     152
 
StyI C'CwwG_G

Cuts at:      0     584    1238    1251 
   Size:        584     654      13

  Fragments arranged by size: 

                 654     584      13
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     627     887    1251 
   Size:        627     260     364

  Fragments arranged by size: 

                 627     364     260
 
TatI w'GTAC_w

Cuts at:      0     512     809    1251 
   Size:        512     297     442

  Fragments arranged by size: 

                 512     442     297
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     763     795    1251 
   Size:        763      32     456

  Fragments arranged by size: 

                 763     456      32
 

 Enzymes that do cut:

     AccI    AflII     AlfI    AlwNI     ApoI     AvaI    BamHI     BanI
     BbsI    BbvCI     BclI     BglI Bme1580I    BmgBI     BmrI     BplI
   Bpu10I    BsaWI    BseRI    BseYI     BsgI    BsmBI Bsp1286I    BspMI
    BsrBI    BsrGI    BstYI   Bsu36I     ClaI     EaeI     EarI    EcoNI
    Hin4I   MspA1I     NspI    PflMI     PsrI     SfiI     SmlI     StyI
    TaqII     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI
     AhdI     AleI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BanII     BbeI     BcgI    BciVI    BfrBI    BglII
     BlpI     BmtI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaXI    BsiEI  BsiHKAI    BsiWI     BsmI    BspEI    BspHI    BsrDI
    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
     BtgI    BtgZI     BtsI    CspCI     DraI   DraIII     DrdI     EagI
     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI
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