Restriction map for
pFN21AB5012
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# NO CUT ENZYMES ( 128 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BalI BanI BanII
BbeI BbvCI BclI BfrI BlnI
BlpI Bpu1102I BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
BsoBI BspEI BspLU11I BsrDI BssHII
BstAPI BstBI BstEII BstPI BstUI
CelII CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI Hin1I HindIII
HpaI HphI KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PshAI PshBI Psp1406I
PstI PvuI RsrII SacI SacII
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tth111I Van91I XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AcsI 367 AAATTC [GA]^AATT[CT]
AocI 1477 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1406 AGCGCT AGC^GCT
ApoI 367 AAATTC [GA]^AATT[CT]
BamHI 99 GGATCC G^GATCC
BbrPI 112 CACGTG CAC^GTG
BbsI 880 GAAGAC GAAGAC(2/6)
BciVI 1259 GTATCC GTATCC(6/5)
BglI 349 GCCAGGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 648 AGATCT A^GATCT
BpuAI 880 GAAGAC GAAGAC(2/6)
BsaAI 112 CACGTG [CT]AC^GT[GA]
BsaI 622 GAGACC GGTCTC(1/5)
BsgI 1483 GTGCAG GTGCAG(16/14)
BsmFI 1121 GGGAC GGGAC(10/14)
Bsp1407I 119 TGTACA T^GTACA
BspDI 58 ATCGAT AT^CGAT
BspMI 6 GCAGGT ACCTGC(4/8)
BsrBI 1048 GAGCGG CCGCTC(-3/-3)
BsrFI 1430 ACCGGC [GA]^CCGG[CT]
BsrGI 119 TGTACA T^GTACA
BsrI 209 ACTGG ACTGG(1/-1)
BssSI 301 CACGAG CACGAG(-5/-1)
Bst1107I 802 GTATAC GTA^TAC
BstZ17I 802 GTATAC GTA^TAC
Bsu36I 1477 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1430 ACCGGC [GA]^CCGG[CT]
ClaI 58 ATCGAT AT^CGAT
EaeI 1469 TGGCCG [CT]^GGCC[GA]
EarI 580 GAAGAG CTCTTC(1/4)
Eco47III 1406 AGCGCT AGC^GCT
Eco81I 1477 CCTGAGG CC^T[ACGT]AGG
HgaI 539 GCGTC GACGC(5/10)
HincII 193 GTCGAC GT[CT]^[GA]AC
HindII 193 GTCGAC GT[CT]^[GA]AC
Ksp632I 580 GAAGAG CTCTTC(1/4)
MaeII 113 ACGT A^CGT
NdeI 905 CATATG CA^TATG
NspI 386 ACATGC [GA]CATG^[CT]
PleI 1372 GACTC GAGTC(4/5)
PmaCI 112 CACGTG CAC^GTG
PmlI 112 CACGTG CAC^GTG
PpuMI 1538 AGGACCC [GA]G^G[AT]CC[CT]
SalI 193 GTCGAC G^TCGAC
SspBI 119 TGTACA T^GTACA
TfiI 1569 GAATC G^A[AT]TC
Tsp45I 1499 GTGAC ^GT[GC]AC
XbaI 243 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 193 GTCGAC GT^[AC][GT]AC
802 GTATAC
AlwNI 670 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
1594 CAGAAGCTG
Asp700I 375 GAACCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
646 GAAGATCTTC
AspHI 985 GAGCAC G[AT]GC[AT]^C
1003 GTGCTC
AvaII 762 GGACC G^G[AT]CC
1539 GGACC
BcgI 1198 CGAGCTGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
185 GCAAGACTGTCG
BfaI 244 CTAG C^TAG
1363 CTAG
BsiHKAI 985 GAGCAC G[AT]GC[AT]^C
1003 GTGCTC
DraII 38 AGGCCCT [GA]G^G[ACGT]CC[CT]
1538 AGGACCC
DsaI 1340 CCATGG C^C[GA][CT]GG
1466 CCATGG
EcoO109I 38 AGGCCCT [GA]G^G[ACGT]CC[CT]
1538 AGGACCC
HaeII 1359 GGCGCT [GA]GCGC^[CT]
1406 AGCGCT
HinfI 1372 GACTC G^A[ACGT]TC
1569 GAATC
MaeI 244 CTAG C^TAG
1363 CTAG
MaeIII 53 GTAAC ^GT[ACGT]AC
1499 GTGAC
NcoI 1340 CCATGG C^CATGG
1466 CCATGG
SmlI 1006 CTCAAG C^T[CT][GA]AG
1240 CTCAAG
XmnI 375 GAACCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
646 GAAGATCTTC
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# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BcnI BmyI BpmI BsaJI BseRI
BsiYI BslI BsmAI Bsp1286I BspHI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII ItaI
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TaqI Tru9I TseI
Tsp509I TspRI TthHB8I XhoII