(Circular) (Six-base) MAPSORT of: pFN21AB5012.seq Check: 1941 from: 1 to: 6334 pFN21AB5012 6334 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4144 278 Size: 53 83 186 936 2608 2468 Fragments arranged by size: 2608 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2199 2808 3677 1937 Size: 262 609 869 4594 Fragments arranged by size: 4594 869 609 262 Acc65I G'GTAC_C Cuts at: 3655 3655 Size: 6334 AclI AA'CG_TT Cuts at: 1563 4461 4834 1563 Size: 2898 373 3063 Fragments arranged by size: 3063 2898 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2045 3062 4397 5781 6049 1632 Size: 413 1017 1335 1384 268 1917 Fragments arranged by size: 1917 1384 1335 1017 413 268 AfeI AGC'GCT Cuts at: 3413 3413 Size: 6334 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6137 AflIII A'CryG_T Cuts at: 1203 5233 6210 1203 Size: 4030 977 1327 Fragments arranged by size: 4030 1327 977 AgeI A'CCGG_T Cuts at: 5165 5165 Size: 6334 AhdI GACnn_n'nnGTC Cuts at: 5063 5063 Size: 6334 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6334 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6334 AlwNI CAG_nnn'CTG Cuts at: 2680 3604 4077 5649 2680 Size: 924 473 1572 3365 Fragments arranged by size: 3365 1572 924 473 ApaLI G'TGCA_C Cuts at: 4391 5547 4391 Size: 1156 5178 ApoI r'AATT_y Cuts at: 1493 2372 3643 3869 4021 6130 1493 Size: 879 1271 226 152 2109 1697 Fragments arranged by size: 2109 1697 1271 879 226 152 AseI AT'TA_AT Cuts at: 160 1032 4888 160 Size: 872 3856 1606 Fragments arranged by size: 3856 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6334 AvaI C'yCGr_G Cuts at: 1870 1943 3659 1870 Size: 73 1716 4545 Fragments arranged by size: 4545 1716 73 BamHI G'GATC_C Cuts at: 2104 3664 4031 2104 Size: 1560 367 4407 Fragments arranged by size: 4407 1560 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3655 5110 618 Size: 325 217 627 1868 1455 1842 Fragments arranged by size: 1868 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3653 1391 Size: 2262 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2892 928 Size: 971 993 4370 Fragments arranged by size: 4370 993 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2179 2213 3192 3226 4525 4559 1903 Size: 34 242 34 979 34 1299 34 3678 Fragments arranged by size: 3678 1299 979 242 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3275 4226 5436 3275 Size: 951 1210 4173 Fragments arranged by size: 4173 1210 951 BclI T'GATC_A Cuts at: 1548 1548 Size: 6334 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2360 4945 136 Size: 107 122 71 631 605 688 2585 1525 Fragments arranged by size: 2585 1525 688 631 605 122 107 71 BglII A'GATC_T Cuts at: 2653 6329 2653 Size: 3676 2658 BlpI GC'TnA_GC Cuts at: 3751 3751 Size: 6334 Bme1580I G_kGCm'C Cuts at: 1790 2507 4395 5551 1790 Size: 717 1888 1156 2573 Fragments arranged by size: 2573 1888 1156 717 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6334 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2224 4065 5023 Size: 416 53 83 399 24 807 1841 958 Cuts at: 5023 442 Size: 1753 Fragments arranged by size: 1841 1753 958 807 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6334 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3248 3293 3364 4994 1239 Size: 2009 45 71 1630 2579 Fragments arranged by size: 2579 2009 1630 71 45 Bpu10I CC'TnA_GC Cuts at: 2048 2048 Size: 6334 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2996 3230 4459 5324 5622 2996 Size: 234 1229 865 298 3708 Fragments arranged by size: 3708 1229 865 298 234 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2621 4997 882 Size: 617 1122 2376 2219 Fragments arranged by size: 2376 2219 1122 617 BsaAI yAC'GTr Cuts at: 493 1204 2119 4125 493 Size: 711 915 2006 2702 Fragments arranged by size: 2702 2006 915 711 BsaBI GATnn'nnATC Cuts at: 6328 6328 Size: 6334 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4141 4523 5158 275 Size: 53 83 186 936 2608 382 635 1451 Fragments arranged by size: 2608 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4767 5165 5439 5586 5997 4767 Size: 398 274 147 411 5104 Fragments arranged by size: 5104 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2342 2372 3362 3392 1178 Size: 30 165 30 939 30 990 30 4120 Fragments arranged by size: 4120 990 939 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2306 2594 3104 1186 Size: 1120 288 510 4416 Fragments arranged by size: 4416 1120 510 288 BseYI C'CCAG_C Cuts at: 2695 3508 5537 6033 2695 Size: 813 2029 496 2996 Fragments arranged by size: 2996 2029 813 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3509 1163 Size: 573 1773 3988 Fragments arranged by size: 3988 1773 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3784 4545 5573 1540 Size: 275 186 1783 761 1028 2301 Fragments arranged by size: 2301 1783 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2994 3012 4395 4480 5551 2994 Size: 18 1383 85 1071 3777 Fragments arranged by size: 3777 1383 1071 85 18 BsmI GAATG_Cn' Cuts at: 3845 3938 3845 Size: 93 6241 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2507 2994 3012 3653 4395 4480 5551 Size: 399 717 487 18 641 742 85 1071 Cuts at: 5551 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 717 641 487 399 85 18 BspEI T'CCGG_A Cuts at: 5997 5997 Size: 6334 BspHI T'CATG_A Cuts at: 2376 2529 3498 4223 5953 2376 Size: 153 969 725 1730 2757 Fragments arranged by size: 2757 1730 969 725 153 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2002 3689 844 Size: 1158 1687 3489 Fragments arranged by size: 3489 1687 1158 BsrBI CCG'CTC Cuts at: 1394 1823 3055 4221 1394 Size: 429 1232 1166 3507 Fragments arranged by size: 3507 1232 1166 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4829 5003 65 Size: 1183 3581 174 1396 Fragments arranged by size: 3581 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3435 4978 5165 1700 Size: 110 1625 1543 187 2869 Fragments arranged by size: 2869 1625 1543 187 110 BsrGI T'GTAC_A Cuts at: 96 2124 96 Size: 2028 4306 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6334 BssSI C'ACGA_G Cuts at: 2306 4394 5406 2306 Size: 2088 1012 3234 Fragments arranged by size: 3234 2088 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6334 BstBI TT'CG_AA Cuts at: 5138 5138 Size: 6334 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6334 BstXI CCAn_nnnn'nTGG Cuts at: 2764 2866 3226 3346 2764 Size: 102 360 120 5752 Fragments arranged by size: 5752 360 120 102 BstYI r'GATC_y Cuts at: 1972 2104 2518 2653 3594 3664 4031 4416 4433 Size: 132 414 135 941 70 367 385 17 Cuts at: 4433 5885 5971 5983 6329 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 941 414 385 367 346 135 132 86 70 17 12 BstZ17I GTA'TAC Cuts at: 2809 2809 Size: 6334 Bsu36I CC'TnA_GG Cuts at: 3483 3483 Size: 6334 BtgI C'CryG_G Cuts at: 513 1065 2004 3345 3471 513 Size: 552 939 1341 126 3376 Fragments arranged by size: 3376 1341 939 552 126 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3338 487 Size: 683 56 2112 3483 Fragments arranged by size: 3483 2112 683 56 BtsI GCAGTG_nn' Cuts at: 3850 4644 4664 3850 Size: 794 20 5520 Fragments arranged by size: 5520 794 20 ClaI AT'CG_AT Cuts at: 1554 2064 1554 Size: 510 5824 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6299 DraI TTT'AAA Cuts at: 3638 3638 Size: 6334 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5341 809 Size: 507 4025 1802 Fragments arranged by size: 4025 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3474 3781 4670 Size: 54 1425 50 275 18 1644 307 889 Cuts at: 4670 6299 8 Size: 1629 43 Fragments arranged by size: 1644 1629 1425 889 307 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3781 1537 Size: 275 1969 4090 Fragments arranged by size: 4090 1969 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2580 4264 1353 Size: 1227 1684 3423 Fragments arranged by size: 3423 1684 1227 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4921 5295 5441 387 Size: 1064 138 3332 374 146 1280 Fragments arranged by size: 3332 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2045 3062 3248 3293 3364 4397 4994 Size: 393 413 1017 186 45 71 1033 597 Cuts at: 4994 5781 6049 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1033 1017 787 597 413 393 268 186 71 45 EcoO109I rG'GnC_Cy Cuts at: 2044 3544 2044 Size: 1500 4834 EcoRI G'AATT_C Cuts at: 3643 4021 3643 Size: 378 5956 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2672 2740 2672 Size: 68 6266 FspI TGC'GCA Cuts at: 4840 4840 Size: 6334 HaeII r_GCGC'y Cuts at: 1678 3368 3415 5481 1678 Size: 1690 47 2066 2531 Fragments arranged by size: 2531 2066 1690 47 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3362 3394 4975 5007 5049 Size: 32 135 32 1790 32 1581 32 42 Cuts at: 5049 5081 1373 Size: 32 2626 Fragments arranged by size: 2626 1790 1581 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2200 3678 3924 669 Size: 988 281 262 1478 246 3079 Fragments arranged by size: 3079 1478 988 281 262 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6334 HpaI GTT'AAC Cuts at: 3924 3924 Size: 6334 KpnI G_GTAC'C Cuts at: 3659 3659 Size: 6334 MfeI C'AATT_G Cuts at: 3933 3933 Size: 6334 MluI A'CGCG_T Cuts at: 6210 6210 Size: 6334 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2394 5448 5632 1305 Size: 1089 3054 184 2007 Fragments arranged by size: 3054 2007 1089 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4512 Fragments arranged by size: 4512 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2777 2864 3350 4292 4651 4810 518 Size: 648 1611 87 486 942 359 159 2042 Fragments arranged by size: 2042 1611 942 648 486 359 159 87 MspA1I CmG'CkG Cuts at: 1606 2804 3238 3271 3311 3384 3750 4427 5575 Size: 1198 434 33 40 73 366 677 1148 Cuts at: 5575 5820 1606 Size: 245 2120 Fragments arranged by size: 2120 1198 1148 677 434 366 245 73 40 33 NcoI C'CATG_G Cuts at: 513 1065 2004 3345 3471 513 Size: 552 939 1341 126 3376 Fragments arranged by size: 3376 1341 939 552 126 NdeI CA'TA_TG Cuts at: 387 2911 387 Size: 2524 3810 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6334 NotI GC'GGCC_GC Cuts at: 3781 3781 Size: 6334 NspI r_CATG'y Cuts at: 1132 1229 2395 3692 5237 1132 Size: 97 1166 1297 1545 2229 Fragments arranged by size: 2229 1545 1297 1166 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6334 PciI A'CATG_T Cuts at: 5233 5233 Size: 6334 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6334 PfoI T'CCnGG_A Cuts at: 3225 3525 3225 Size: 300 6034 PmeI GTTT'AAAC Cuts at: 3638 3638 Size: 6334 PmlI CAC'GTG Cuts at: 2119 2119 Size: 6334 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4403 4435 5916 5948 4403 Size: 32 1481 32 4789 Fragments arranged by size: 4789 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3544 3544 Size: 6334 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6334 PsiI TTA'TAA Cuts at: 3904 3904 Size: 6334 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2693 2725 2693 Size: 32 6302 PstI C_TGCA'G Cuts at: 830 3686 830 Size: 2856 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6334 PvuII CAG'CTG Cuts at: 3238 3271 3384 3238 Size: 33 113 6188 Fragments arranged by size: 6188 113 33 SalI G'TCGA_C Cuts at: 1936 2198 3676 1936 Size: 262 1478 4594 Fragments arranged by size: 4594 1478 262 SbfI CC_TGCA'GG Cuts at: 3686 3686 Size: 6334 ScaI AGT'ACT Cuts at: 4582 6294 4582 Size: 1712 4622 SfcI C'TryA_G Cuts at: 826 1045 3682 4817 5498 5689 826 Size: 219 2637 1135 681 191 1471 Fragments arranged by size: 2637 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6334 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6334 SmaI CCC'GGG Cuts at: 1872 3661 1872 Size: 1789 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3011 3245 4438 5339 5601 820 Size: 197 926 1068 234 1193 901 262 1553 Fragments arranged by size: 1553 1193 1068 926 901 262 234 197 SnaBI TAC'GTA Cuts at: 493 4125 493 Size: 3632 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6334 SphI G_CATG'C Cuts at: 1132 3692 1132 Size: 2560 3774 SspI AAT'ATT Cuts at: 5 52 4258 5 Size: 47 4206 2081 Fragments arranged by size: 4206 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6334 StyI C'CwwG_G Cuts at: 513 1065 2004 2551 2757 3345 3471 3773 513 Size: 552 939 547 206 588 126 302 3074 Fragments arranged by size: 3074 939 588 552 547 302 206 126 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4361 4378 4531 4716 5161 325 Size: 1255 2781 17 153 185 445 1498 Fragments arranged by size: 2781 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2124 2949 3429 Size: 274 80 33 51 1443 147 825 480 Cuts at: 3429 4580 6292 96 Size: 1151 1712 138 Fragments arranged by size: 1712 1443 1151 825 480 274 147 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3579 3611 6294 6326 3579 Size: 32 2683 32 3587 Fragments arranged by size: 3587 2683 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6334 XbaI T'CTAG_A Cuts at: 2248 3670 2248 Size: 1422 4912 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6334 XmaI C'CCGG_G Cuts at: 1870 3659 1870 Size: 1789 4545 XmnI GAAnn'nnTTC Cuts at: 2384 2655 4463 2384 Size: 271 1808 4255 Fragments arranged by size: 4255 1808 271 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4142 276 Size: 53 83 186 936 2608 2468 Fragments arranged by size: 2608 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI