Restriction map for
pFN21AB5010
(Linear) (Six-base) MAPSORT of: KIBB5010.seq Check: 555 from: 1 to: 1857
KIBB5010.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1306 1413 1726 1857
Size: 1306 107 313 131
Fragments arranged by size:
1306 313 131 107
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 170 524 1857
Size: 170 354 1333
Fragments arranged by size:
1333 354 170
AfeI AGC'GCT
Cuts at: 0 49 1606 1857
Size: 49 1557 251
Fragments arranged by size:
1557 251 49
AleI CACnn'nnGTG
Cuts at: 0 476 980 1857
Size: 476 504 877
Fragments arranged by size:
877 504 476
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 401 433 1857
Size: 401 32 1424
Fragments arranged by size:
1424 401 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1281 1857
Size: 1281 576
ApaLI G'TGCA_C
Cuts at: 0 385 985 1069 1153 1857
Size: 385 600 84 84 704
Fragments arranged by size:
704 600 385 84 84
AvaI C'yCGr_G
Cuts at: 0 51 289 343 822 876 1158 1857
Size: 51 238 54 479 54 282 699
Fragments arranged by size:
699 479 282 238 54 54 51
AvrII C'CTAG_G
Cuts at: 0 241 1857
Size: 241 1616
BanI G'GyrC_C
Cuts at: 0 285 1857
Size: 285 1572
BanII G_rGCy'C
Cuts at: 0 623 1622 1817 1857
Size: 623 999 195 40
Fragments arranged by size:
999 623 195 40
BglI GCCn_nnn'nGGC
Cuts at: 0 488 1336 1857
Size: 488 848 521
Fragments arranged by size:
848 521 488
Bme1580I G_kGCm'C
Cuts at: 0 343 389 847 989 1073 1157 1268 1790
Size: 343 46 458 142 84 84 111 522
Cuts at: 1790 1857
Size: 67
Fragments arranged by size:
522 458 343 142 111 84 84 67
46
BmrI ACTGGGnnnn_n'
Cuts at: 0 1536 1635 1857
Size: 1536 99 222
Fragments arranged by size:
1536 222 99
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1590 1857
Size: 1590 267
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1349 1831 1857
Size: 1349 482 26
Fragments arranged by size:
1349 482 26
Bpu10I CC'TnA_GC
Cuts at: 0 191 1857
Size: 191 1666
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 607 1036 1764 1857
Size: 448 159 429 728 93
Fragments arranged by size:
728 448 429 159 93
BsaI GGTCTCn'nnnn_
Cuts at: 0 1561 1857
Size: 1561 296
BsaAI yAC'GTr
Cuts at: 0 719 1857
Size: 719 1138
BsaHI Gr'CG_yC
Cuts at: 0 1039 1857
Size: 1039 818
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 205 235 1857
Size: 205 30 1622
Fragments arranged by size:
1622 205 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1114 1534 1857
Size: 1114 420 323
Fragments arranged by size:
1114 420 323
BseYI C'CCAG_C
Cuts at: 0 30 1353 1669 1857
Size: 30 1323 316 188
Fragments arranged by size:
1323 316 188 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1256 1620 1857
Size: 1256 364 237
Fragments arranged by size:
1256 364 237
BsiEI CG_ry'CG
Cuts at: 0 354 858 999 1003 1857
Size: 354 504 141 4 854
Fragments arranged by size:
854 504 354 141 4
BsiHKAI G_wGCw'C
Cuts at: 0 389 623 989 1073 1157 1622 1857
Size: 389 234 366 84 84 465 235
Fragments arranged by size:
465 389 366 235 234 84 84
BsmI GAATG_Cn'
Cuts at: 0 1459 1841 1857
Size: 1459 382 16
Fragments arranged by size:
1459 382 16
Bsp1286I G_dGCh'C
Cuts at: 0 343 389 623 847 989 1073 1157 1268
Size: 343 46 234 224 142 84 84 111
Cuts at: 1268 1622 1790 1817 1857
Size: 354 168 27 40
Fragments arranged by size:
354 343 234 224 168 142 111 84
84 46 40 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1306 1413 1726 1857
Size: 1306 107 313 131
Fragments arranged by size:
1306 313 131 107
BsrBI CCG'CTC
Cuts at: 0 495 645 704 1233 1857
Size: 495 150 59 529 624
Fragments arranged by size:
624 529 495 150 59
BsrDI GCAATG_nn'
Cuts at: 0 1024 1857
Size: 1024 833
BsrFI r'CCGG_y
Cuts at: 0 135 557 655 957 977 998 1851 1857
Size: 135 422 98 302 20 21 853 6
Fragments arranged by size:
853 422 302 135 98 21 20 6
BssHII G'CGCG_C
Cuts at: 0 112 449 897 1857
Size: 112 337 448 960
Fragments arranged by size:
960 448 337 112
BssSI C'ACGA_G
Cuts at: 0 808 1228 1600 1857
Size: 808 420 372 257
Fragments arranged by size:
808 420 372 257
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1643 1857
Size: 1643 214
BstXI CCAn_nnnn'nTGG
Cuts at: 0 478 1465 1809 1857
Size: 478 987 344 48
Fragments arranged by size:
987 478 344 48
BstYI r'GATC_y
Cuts at: 0 467 1857
Size: 467 1390
Bsu36I CC'TnA_GG
Cuts at: 0 1219 1857
Size: 1219 638
BtgI C'CryG_G
Cuts at: 0 230 303 1256 1790 1857
Size: 230 73 953 534 67
Fragments arranged by size:
953 534 230 73 67
BtsI GCAGTG_nn'
Cuts at: 0 429 924 1254 1857
Size: 429 495 330 603
Fragments arranged by size:
603 495 429 330
DraIII CAC_nnn'GTG
Cuts at: 0 1418 1643 1785 1857
Size: 1418 225 142 72
Fragments arranged by size:
1418 225 142 72
EaeI y'GGCC_r
Cuts at: 0 158 233 351 480 855 968 1000 1077
Size: 158 75 118 129 375 113 32 77
Cuts at: 1077 1409 1857
Size: 332 448
Fragments arranged by size:
448 375 332 158 129 118 113 77
75 32
EagI C'GGCC_G
Cuts at: 0 351 855 1000 1857
Size: 351 504 145 857
Fragments arranged by size:
857 504 351 145
EarI CTCTTCn'nnn_
Cuts at: 0 146 685 1838 1857
Size: 146 539 1153 19
Fragments arranged by size:
1153 539 146 19
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 976 1857
Size: 976 881
EcoICRI GAG'CTC
Cuts at: 0 621 1620 1857
Size: 621 999 237
Fragments arranged by size:
999 621 237
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 170 524 1349 1831 1857
Size: 170 354 825 482 26
Fragments arranged by size:
825 482 354 170 26
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1367 1857
Size: 1367 490
EcoO109I rG'GnC_Cy
Cuts at: 0 245 627 1047 1359 1796 1857
Size: 245 382 420 312 437 61
Fragments arranged by size:
437 420 382 312 245 61
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1587 1758 1857
Size: 1587 171 99
Fragments arranged by size:
1587 171 99
FseI GG_CCGG'CC
Cuts at: 0 1855 1857
Size: 1855 2
FspI TGC'GCA
Cuts at: 0 298 428 1678 1762 1857
Size: 298 130 1250 84 95
Fragments arranged by size:
1250 298 130 95 84
FspAI rTGC'GCAy
Cuts at: 0 428 1857
Size: 428 1429
HaeII r_GCGC'y
Cuts at: 0 51 666 1170 1300 1608 1857
Size: 51 615 504 130 308 249
Fragments arranged by size:
615 504 308 249 130 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 10 42 205 237 870 902 1857
Size: 10 32 163 32 633 32 955
Fragments arranged by size:
955 633 163 32 32 32 10
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 893 1857
Size: 893 964
MscI TGG'CCA
Cuts at: 0 482 970 1079 1411 1857
Size: 482 488 109 332 446
Fragments arranged by size:
488 482 446 332 109
MslI CAynn'nnrTG
Cuts at: 0 476 980 1438 1857
Size: 476 504 458 419
Fragments arranged by size:
504 476 458 419
MspA1I CmG'CkG
Cuts at: 0 232 422 590 758 1083 1094 1258 1857
Size: 232 190 168 168 325 11 164 599
Fragments arranged by size:
599 325 232 190 168 168 164 11
NaeI GCC'GGC
Cuts at: 0 137 959 1853 1857
Size: 137 822 894 4
Fragments arranged by size:
894 822 137 4
NcoI C'CATG_G
Cuts at: 0 1790 1857
Size: 1790 67
NgoMIV G'CCGG_C
Cuts at: 0 135 957 1851 1857
Size: 135 822 894 6
Fragments arranged by size:
894 822 135 6
NotI GC'GGCC_GC
Cuts at: 0 351 855 1857
Size: 351 504 1002
Fragments arranged by size:
1002 504 351
NspI r_CATG'y
Cuts at: 0 864 1190 1450 1857
Size: 864 326 260 407
Fragments arranged by size:
864 407 326 260
PasI CC'CwG_GG
Cuts at: 0 104 1857
Size: 104 1753
PflMI CCAn_nnn'nTGG
Cuts at: 0 1418 1857
Size: 1418 439
PfoI T'CCnGG_A
Cuts at: 0 1472 1857
Size: 1472 385
PmlI CAC'GTG
Cuts at: 0 719 1857
Size: 719 1138
PpuMI rG'GwC_Cy
Cuts at: 0 627 1047 1359 1796 1857
Size: 627 420 312 437 61
Fragments arranged by size:
627 437 420 312 61
PspXI vC'TCGA_Gb
Cuts at: 0 876 1857
Size: 876 981
PstI C_TGCA'G
Cuts at: 0 18 1857
Size: 18 1839
PvuII CAG'CTG
Cuts at: 0 590 1857
Size: 590 1267
SacI G_AGCT'C
Cuts at: 0 623 1622 1857
Size: 623 999 235
Fragments arranged by size:
999 623 235
SacII CC_GC'GG
Cuts at: 0 233 1259 1857
Size: 233 1026 598
Fragments arranged by size:
1026 598 233
SanDI GG'GwC_CC
Cuts at: 0 1359 1796 1857
Size: 1359 437 61
Fragments arranged by size:
1359 437 61
SapI GCTCTTCn'nnn_
Cuts at: 0 685 1857
Size: 685 1172
SfcI C'TryA_G
Cuts at: 0 14 585 1857
Size: 14 571 1272
Fragments arranged by size:
1272 571 14
SgrAI Cr'CCGG_yG
Cuts at: 0 557 655 977 1857
Size: 557 98 322 880
Fragments arranged by size:
880 557 322 98
SmlI C'TyrA_G
Cuts at: 0 463 622 876 1051 1743 1857
Size: 463 159 254 175 692 114
Fragments arranged by size:
692 463 254 175 159 114
StuI AGG'CCT
Cuts at: 0 274 442 1738 1857
Size: 274 168 1296 119
Fragments arranged by size:
1296 274 168 119
StyI C'CwwG_G
Cuts at: 0 241 269 1490 1733 1790 1857
Size: 241 28 1221 243 57 67
Fragments arranged by size:
1221 243 241 67 57 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1654 1792 1857
Size: 1654 138 65
Fragments arranged by size:
1654 138 65
XhoI C'TCGA_G
Cuts at: 0 876 1857
Size: 876 981
XmnI GAAnn'nnTTC
Cuts at: 0 694 835 1857
Size: 694 141 1022
Fragments arranged by size:
1022 694 141
Enzymes that do cut:
AarI AcuI AfeI AleI AloI AlwNI ApaLI AvaI
AvrII BanI BanII BglI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstXI BstYI Bsu36I BtgI
BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FalI FseI FspI FspAI HaeII Hin4I
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NotI
NspI PasI PflMI PfoI PmlI PpuMI PspXI PstI
PvuII SacI SacII SanDI SapI SfcI SgrAI SmlI
StuI StyI TaqII XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AlfI ApaI ApoI AscI AseI AsiSI BaeI BamHI
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI BmgBI BmtI BsaBI BsaWI BsiWI BsmBI BspEI
BspHI BsrGI BstBI BstEII BstZ17I BtgZI ClaI CspCI
DraI DrdI EcoRI EcoRV HincII HindIII HpaI KasI
KpnI MfeI MluI NarI NdeI NheI NruI NsiI
PacI PciI PmeI PpiI PshAI PsiI PspOMI PsrI
PvuI RsrII SalI SbfI ScaI SexAI SfiI SfoI
SgfI SmaI SnaBI SpeI SphI SrfI SspI SwaI
TatI TstI Tth111I XbaI XcmI XmaI ZraI