Restriction map for
pFN21AB5006
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BbrPI BbsI BcgI
BciVI BclI BfrI BlnI BpuAI
BsaAI BsaBI BsaWI BseAI BsgI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HgaI HincII HindII HpaI
KpnI KspI MaeII MamI MfeI
MluI MroI MunI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tsp45I Van91I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccII 469 CGCG CG^CG
AcyI 45 GGCGCC G[GA]^CG[CT]C
AocI 1289 CCTCAGG CC^T[ACGT]AGG
AspI 151 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1214 CCCGGG C^[CT]CG[GA]G
BalI 974 TGGCCA TGG^CCA
BamHI 1196 GGATCC G^GATCC
BanI 45 GGCGCC G^G[CT][GA]CC
BanII 559 GAGCTC G[GA]GC[CT]^C
BbeI 45 GGCGCC GGCGC^C
BbvCI 1255 GCTGAGG CCTCAGC(-5/-2)
BglI 1084 GCCCACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 420 AGATCT A^GATCT
BsaHI 45 GGCGCC G[GA]^CG[CT]C
BsaI 1295 GAGACC GGTCTC(1/5)
BsiEI 23 CGACCG CG[GA][CT]^CG
BsoBI 1214 CCCGGG C^[CT]CG[GA]G
BspMI 316 ACCTGC ACCTGC(4/8)
BsrDI 273 CATTGC GCAATG(2/0)
BstF5I 1300 CATCC GGATG(2/0)
BstUI 469 CGCG CG^CG
Bsu36I 1289 CCTCAGG CC^T[ACGT]AGG
DsaI 56 CCATGG C^C[GA][CT]GG
Eco81I 1289 CCTCAGG CC^T[ACGT]AGG
EcoRI 783 GAATTC G^AATTC
FokI 1300 CATCC GGATG(9/13)
HaeII 45 GGCGCC [GA]GCGC^[CT]
Hin1I 45 GGCGCC G[GA]^CG[CT]C
HindIII 863 AAGCTT A^AGCTT
KasI 45 GGCGCC G^GCGCC
MaeIII 1025 GTAAC ^GT[ACGT]AC
MluNI 974 TGGCCA TGG^CCA
MscI 974 TGGCCA TGG^CCA
MseI 410 TTAA T^TAA
MvnI 469 CGCG CG^CG
NarI 45 GGCGCC GG^CGCC
NcoI 56 CCATGG C^CATGG
PflFI 151 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1133 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 202 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1138 CTGCAG CTGCA^G
PvuII 1174 CAGCTG CAG^CTG
SacI 559 GAGCTC GAGCT^C
SapI 1332 GAAGAGC GCTCTTC(1/4)
SfaNI 135 GATGC GCATC(5/9)
SfiI 1083 GGCCCACCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 45 GGCGCC GGC^GCC
SmaI 1214 CCCGGG CCC^GGG
StuI 998 AGGCCT AGG^CCT
TaqI 951 TCGA T^CGA
Tru9I 410 TTAA T^TAA
Tth111I 151 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 951 TCGA T^CGA
XmaCI 1214 CCCGGG C^CCGGG
XmaI 1214 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcsI 226 AAATTC [GA]^AATT[CT]
783 GAATTC
ApoI 226 AAATTC [GA]^AATT[CT]
783 GAATTC
AspHI 559 GAGCTC G[AT]GC[AT]^C
807 GAGCAC
AvaII 150 GGACC G^G[AT]CC
1134 GGTCC
BfaI 911 CTAG C^TAG
1036 CTAG
BlpI 484 GCTAAGC GC^T[ACGT]AGC
721 GCTGAGC
BpmI 204 CTCCAG CTGGAG(16/14)
610 CTGGAG
Bpu1102I 484 GCTAAGC GC^T[ACGT]AGC
721 GCTGAGC
BsiHKAI 559 GAGCTC G[AT]GC[AT]^C
807 GAGCAC
BsmFI 149 GGGAC GGGAC(10/14)
269 GGGAC
BsrBI 30 GAGCGG CCGCTC(-3/-3)
823 GAGCGG
BstXI 165 CCACTACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
287 CCATGAAGGTGG
CelII 484 GCTAAGC GC^T[ACGT]AGC
721 GCTGAGC
EaeI 974 TGGCCA [CT]^GGCC[GA]
1091 TGGCCG
HapII 1074 CCGG C^CGG
1215 CCGG
HpaII 1074 CCGG C^CGG
1215 CCGG
MaeI 911 CTAG C^TAG
1036 CTAG
MspI 1074 CCGG C^CGG
1215 CCGG
SmlI 661 CTCAAG C^T[CT][GA]AG
745 CTCAAG
TfiI 363 GATTC G^A[AT]TC
446 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BcnI BmyI BsaJI BseRI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI HaeIII
HhaI HinP1I HinfI HphI ItaI
Ksp632I MboI MboII MflI MnlI
MslI MspA1I MvaI MwoI NciI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfcI StyI
TseI Tsp509I TspRI XcmI XhoII