Restriction map for
pFN21AB5006
(Linear) (Six-base) MAPSORT of: KIBB5006.seq Check: 3109 from: 1 to: 1377
KIBB5006.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 325 1377
Size: 325 1052
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 1377
Size: 121 1256
AleI CACnn'nnGTG
Cuts at: 0 814 1377
Size: 814 563
ApoI r'AATT_y
Cuts at: 0 226 783 1377
Size: 226 557 594
Fragments arranged by size:
594 557 226
AvaI C'yCGr_G
Cuts at: 0 1214 1377
Size: 1214 163
BamHI G'GATC_C
Cuts at: 0 1196 1377
Size: 1196 181
BanI G'GyrC_C
Cuts at: 0 45 1377
Size: 45 1332
BanII G_rGCy'C
Cuts at: 0 563 1377
Size: 563 814
BbeI G_GCGC'C
Cuts at: 0 49 1377
Size: 49 1328
BbvCI CC'TCA_GC
Cuts at: 0 1256 1377
Size: 1256 121
BglI GCCn_nnn'nGGC
Cuts at: 0 1090 1377
Size: 1090 287
BglII A'GATC_T
Cuts at: 0 420 1377
Size: 420 957
BlpI GC'TnA_GC
Cuts at: 0 485 722 1377
Size: 485 237 655
Fragments arranged by size:
655 485 237
Bme1580I G_kGCm'C
Cuts at: 0 124 832 1377
Size: 124 708 545
Fragments arranged by size:
708 545 124
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1305 1373 1377
Size: 1305 68 4
Fragments arranged by size:
1305 68 4
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 189 631 1377
Size: 189 442 746
Fragments arranged by size:
746 442 189
Bpu10I CC'TnA_GC
Cuts at: 0 1256 1377
Size: 1256 121
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 646 730 1377
Size: 646 84 647
Fragments arranged by size:
647 646 84
BsaI GGTCTCn'nnnn_
Cuts at: 0 1289 1377
Size: 1289 88
BsaHI Gr'CG_yC
Cuts at: 0 46 1377
Size: 46 1331
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 214 244 1377
Size: 214 30 1133
Fragments arranged by size:
1133 214 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 25 1273 1276 1339 1377
Size: 25 1248 3 63 38
Fragments arranged by size:
1248 63 38 25 3
BseYI C'CCAG_C
Cuts at: 0 667 1172 1377
Size: 667 505 205
Fragments arranged by size:
667 505 205
BsiEI CG_ry'CG
Cuts at: 0 26 1377
Size: 26 1351
BsiHKAI G_wGCw'C
Cuts at: 0 563 811 1377
Size: 563 248 566
Fragments arranged by size:
566 563 248
Bsp1286I G_dGCh'C
Cuts at: 0 124 563 811 832 1377
Size: 124 439 248 21 545
Fragments arranged by size:
545 439 248 124 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 325 1377
Size: 325 1052
BsrBI CCG'CTC
Cuts at: 0 32 825 1377
Size: 32 793 552
Fragments arranged by size:
793 552 32
BsrDI GCAATG_nn'
Cuts at: 0 272 1377
Size: 272 1105
BstXI CCAn_nnnn'nTGG
Cuts at: 0 172 294 1377
Size: 172 122 1083
Fragments arranged by size:
1083 172 122
BstYI r'GATC_y
Cuts at: 0 420 594 1196 1377
Size: 420 174 602 181
Fragments arranged by size:
602 420 181 174
Bsu36I CC'TnA_GG
Cuts at: 0 1290 1377
Size: 1290 87
BtgI C'CryG_G
Cuts at: 0 56 1377
Size: 56 1321
BtsI GCAGTG_nn'
Cuts at: 0 690 1377
Size: 690 687
EaeI y'GGCC_r
Cuts at: 0 974 1091 1377
Size: 974 117 286
Fragments arranged by size:
974 286 117
EarI CTCTTCn'nnn_
Cuts at: 0 425 785 1072 1327 1377
Size: 425 360 287 255 50
Fragments arranged by size:
425 360 287 255 50
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 458 1060 1377
Size: 458 602 317
Fragments arranged by size:
602 458 317
EcoICRI GAG'CTC
Cuts at: 0 561 1377
Size: 561 816
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 189 631 1377
Size: 121 68 442 746
Fragments arranged by size:
746 442 121 68
EcoO109I rG'GnC_Cy
Cuts at: 0 945 1032 1062 1134 1377
Size: 945 87 30 72 243
Fragments arranged by size:
945 243 87 72 30
EcoRI G'AATT_C
Cuts at: 0 783 1377
Size: 783 594
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1083 1377
Size: 1083 294
HaeII r_GCGC'y
Cuts at: 0 49 1377
Size: 49 1328
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 56 88 146 178 214 246 1377
Size: 56 32 58 32 36 32 1131
Fragments arranged by size:
1131 58 56 36 32 32 32
HindIII A'AGCT_T
Cuts at: 0 863 1377
Size: 863 514
KasI G'GCGC_C
Cuts at: 0 45 1377
Size: 45 1332
MscI TGG'CCA
Cuts at: 0 976 1377
Size: 976 401
MslI CAynn'nnrTG
Cuts at: 0 292 814 1315 1377
Size: 292 522 501 62
Fragments arranged by size:
522 501 292 62
MspA1I CmG'CkG
Cuts at: 0 51 555 1176 1194 1377
Size: 51 504 621 18 183
Fragments arranged by size:
621 504 183 51 18
NarI GG'CG_CC
Cuts at: 0 46 1377
Size: 46 1331
NcoI C'CATG_G
Cuts at: 0 56 1377
Size: 56 1321
PpuMI rG'GwC_Cy
Cuts at: 0 1134 1377
Size: 1134 243
PshAI GACnn'nnGTC
Cuts at: 0 206 1377
Size: 206 1171
PstI C_TGCA'G
Cuts at: 0 1142 1377
Size: 1142 235
PvuII CAG'CTG
Cuts at: 0 1176 1377
Size: 1176 201
SacI G_AGCT'C
Cuts at: 0 563 1377
Size: 563 814
SapI GCTCTTCn'nnn_
Cuts at: 0 1327 1377
Size: 1327 50
SfcI C'TryA_G
Cuts at: 0 871 994 1138 1377
Size: 871 123 144 239
Fragments arranged by size:
871 239 144 123
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1090 1377
Size: 1090 287
SfoI GGC'GCC
Cuts at: 0 47 1377
Size: 47 1330
SmaI CCC'GGG
Cuts at: 0 1216 1377
Size: 1216 161
SmlI C'TyrA_G
Cuts at: 0 661 745 1377
Size: 661 84 632
Fragments arranged by size:
661 632 84
StuI AGG'CCT
Cuts at: 0 1000 1377
Size: 1000 377
StyI C'CwwG_G
Cuts at: 0 56 764 1274 1377
Size: 56 708 510 103
Fragments arranged by size:
708 510 103 56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 40 167 1377
Size: 40 127 1210
Fragments arranged by size:
1210 127 40
TatI w'GTAC_w
Cuts at: 0 836 1377
Size: 836 541
Tth111I GACn'n_nGTC
Cuts at: 0 154 1377
Size: 154 1223
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 63 291 627 1173 1377
Size: 63 228 336 546 204
Fragments arranged by size:
546 336 228 204 63
XmaI C'CCGG_G
Cuts at: 0 1214 1377
Size: 1214 163
Enzymes that do cut:
AarI AcuI AleI ApoI AvaI BamHI BanI BanII
BbeI BbvCI BglI BglII BlpI Bme1580I BplI BpmI
Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspMI BsrBI BsrDI BstXI BstYI Bsu36I
BtgI BtsI EaeI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI HaeII Hin4I HindIII KasI MscI MslI
MspA1I NarI NcoI PpuMI PshAI PstI PvuII SacI
SapI SfcI SfiI SfoI SmaI SmlI StuI StyI
TaqII TatI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BbsI BcgI BciVI BclI BfrBI
BmgBI BmrI BmtI BsaAI BsaBI BsaWI BsgI BsiWI
BsmI BsmBI BspEI BspHI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI
DraIII DrdI EagI EcoNI EcoRV FseI FspI FspAI
HincII HpaI KpnI MfeI MluI MmeI NaeI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI
ScaI SexAI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI TstI XbaI XhoI XmnI ZraI