(Circular) (Six-base) MAPSORT of: pFN21AB4998.seq Check: 3618 from: 1 to: 5368 pFN21AB4998 5368 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3178 278 Size: 53 83 186 936 1642 2468 Fragments arranged by size: 2468 1642 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2480 2711 1937 Size: 543 231 4594 Fragments arranged by size: 4594 543 231 Acc65I G'GTAC_C Cuts at: 2689 2689 Size: 5368 AclI AA'CG_TT Cuts at: 1563 3495 3868 1563 Size: 1932 373 3063 Fragments arranged by size: 3063 1932 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2664 3431 4815 5083 1632 Size: 1032 767 1384 268 1917 Fragments arranged by size: 1917 1384 1032 767 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5171 AflIII A'CryG_T Cuts at: 1203 2130 4267 5244 1203 Size: 927 2137 977 1327 Fragments arranged by size: 2137 1327 977 927 AgeI A'CCGG_T Cuts at: 4199 4199 Size: 5368 AhdI GACnn_n'nnGTC Cuts at: 4097 4097 Size: 5368 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5368 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5368 AlwNI CAG_nnn'CTG Cuts at: 3111 4683 3111 Size: 1572 3796 ApaLI G'TGCA_C Cuts at: 3425 4581 3425 Size: 1156 4212 ApoI r'AATT_y Cuts at: 1493 2677 2903 3055 5164 1493 Size: 1184 226 152 2109 1697 Fragments arranged by size: 2109 1697 1184 226 152 AseI AT'TA_AT Cuts at: 160 1032 3922 160 Size: 872 2890 1606 Fragments arranged by size: 2890 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5368 AvaI C'yCGr_G Cuts at: 1870 1943 2693 1870 Size: 73 750 4545 Fragments arranged by size: 4545 750 73 AvrII C'CTAG_G Cuts at: 2369 2369 Size: 5368 BamHI G'GATC_C Cuts at: 2698 3065 2698 Size: 367 5001 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2689 4144 618 Size: 325 217 627 902 1455 1842 Fragments arranged by size: 1842 1455 902 627 325 217 BanII G_rGCy'C Cuts at: 1391 2687 1391 Size: 1296 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2095 928 Size: 971 196 4201 Fragments arranged by size: 4201 971 196 BbvCI CC'TCA_GC Cuts at: 2093 2208 2093 Size: 115 5253 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3559 3593 1903 Size: 34 1622 34 3678 Fragments arranged by size: 3678 1622 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3260 4470 3260 Size: 1210 4158 BclI T'GATC_A Cuts at: 1548 1548 Size: 5368 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3979 136 Size: 107 122 71 631 605 2307 1525 Fragments arranged by size: 2307 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2537 5363 2537 Size: 2826 2542 BlpI GC'TnA_GC Cuts at: 2785 2785 Size: 5368 Bme1580I G_kGCm'C Cuts at: 1790 3429 4585 1790 Size: 1639 1156 2573 Fragments arranged by size: 2573 1639 1156 BmgBI CAC'GTC Cuts at: 1318 2131 1318 Size: 813 4555 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2579 3099 4057 Size: 416 53 83 399 24 1162 520 958 Cuts at: 4057 442 Size: 1753 Fragments arranged by size: 1753 1162 958 520 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5368 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2518 4028 1239 Size: 1279 1510 2579 Fragments arranged by size: 2579 1510 1279 Bpu10I CC'TnA_GC Cuts at: 2093 2208 2093 Size: 115 5253 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2036 3493 4358 4656 2036 Size: 1457 865 298 2748 Fragments arranged by size: 2748 1457 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2421 2536 2568 4031 882 Size: 617 922 115 32 1463 2219 Fragments arranged by size: 2219 1463 922 617 115 32 BsaAI yAC'GTr Cuts at: 493 1204 3159 493 Size: 711 1955 2702 Fragments arranged by size: 2702 1955 711 BsaBI GATnn'nnATC Cuts at: 5362 5362 Size: 5368 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2353 2377 3175 3557 Size: 53 83 186 936 820 24 798 382 Cuts at: 3557 4192 275 Size: 635 1451 Fragments arranged by size: 1451 936 820 798 635 382 186 83 53 24 BsaWI w'CCGG_w Cuts at: 3801 4199 4473 4620 5031 3801 Size: 398 274 147 411 4138 Fragments arranged by size: 4138 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5143 Fragments arranged by size: 5143 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5368 BseYI C'CCAG_C Cuts at: 4571 5067 4571 Size: 496 4872 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4795 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2017 2251 2284 2480 2621 2818 Size: 275 186 16 234 33 196 141 197 Cuts at: 2818 3579 4607 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1028 761 275 234 197 196 186 141 33 16 BsiHKAI G_wGCw'C Cuts at: 3429 3514 4585 3429 Size: 85 1071 4212 Fragments arranged by size: 4212 1071 85 BsmI GAATG_Cn' Cuts at: 2879 2972 2879 Size: 93 5275 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2687 3429 3514 4585 1391 Size: 399 897 742 85 1071 2174 Fragments arranged by size: 2174 1071 897 742 399 85 BspEI T'CCGG_A Cuts at: 5031 5031 Size: 5368 BspHI T'CATG_A Cuts at: 3257 4987 3257 Size: 1730 3638 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2319 2723 844 Size: 1475 404 3489 Fragments arranged by size: 3489 1475 404 BsrBI CCG'CTC Cuts at: 1394 1823 2442 2657 3255 1394 Size: 429 619 215 598 3507 Fragments arranged by size: 3507 619 598 429 215 BsrDI GCAATG_nn' Cuts at: 65 1248 3863 4037 65 Size: 1183 2615 174 1396 Fragments arranged by size: 2615 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4012 4199 1700 Size: 110 2202 187 2869 Fragments arranged by size: 2869 2202 187 110 BsrGI T'GTAC_A Cuts at: 96 2133 96 Size: 2037 3331 BssHII G'CGCG_C Cuts at: 1925 2273 1925 Size: 348 5020 BssSI C'ACGA_G Cuts at: 3428 4440 3428 Size: 1012 4356 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5368 BstBI TT'CG_AA Cuts at: 4172 4172 Size: 5368 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5368 BstYI r'GATC_y Cuts at: 1972 2495 2537 2698 3065 3450 3467 4919 5005 Size: 523 42 161 367 385 17 1452 86 Cuts at: 5005 5017 5363 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 523 385 367 346 161 86 42 17 12 BtgI C'CryG_G Cuts at: 513 1065 2141 2167 2226 513 Size: 552 1076 26 59 3655 Fragments arranged by size: 3655 1076 552 59 26 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4629 Fragments arranged by size: 4629 683 56 BtsI GCAGTG_nn' Cuts at: 2884 3678 3698 2884 Size: 794 20 4554 Fragments arranged by size: 4554 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5368 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5333 DraI TTT'AAA Cuts at: 2672 2672 Size: 5368 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2488 4375 809 Size: 507 1172 1887 1802 Fragments arranged by size: 1887 1802 1172 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2014 2248 2281 Size: 54 1425 50 275 18 184 234 33 Cuts at: 2281 2815 3704 5333 8 Size: 534 889 1629 43 Fragments arranged by size: 1629 1425 889 534 275 234 184 54 50 43 33 18 EagI C'GGCC_G Cuts at: 1537 1812 2014 2248 2281 2815 1537 Size: 275 202 234 33 534 4090 Fragments arranged by size: 4090 534 275 234 202 33 EarI CTCTTCn'nnn_ Cuts at: 1353 2618 2640 3298 1353 Size: 1265 22 658 3423 Fragments arranged by size: 3423 1265 658 22 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3955 4329 4475 387 Size: 1064 138 2366 374 146 1280 Fragments arranged by size: 2366 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2518 2664 3431 4028 4815 5083 1239 Size: 393 886 146 767 597 787 268 1524 Fragments arranged by size: 1524 886 787 767 597 393 268 146 EcoO109I rG'GnC_Cy Cuts at: 2556 2556 Size: 5368 EcoRI G'AATT_C Cuts at: 2677 3055 2677 Size: 378 4990 FspI TGC'GCA Cuts at: 3874 3874 Size: 5368 HaeII r_GCGC'y Cuts at: 1678 4515 1678 Size: 2837 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4009 4041 4083 4115 1373 Size: 32 135 32 2437 32 42 32 2626 Fragments arranged by size: 2626 2437 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2481 2712 2958 669 Size: 988 281 543 231 246 3079 Fragments arranged by size: 3079 988 543 281 246 231 HindIII A'AGCT_T Cuts at: 748 748 Size: 5368 HpaI GTT'AAC Cuts at: 2958 2958 Size: 5368 KpnI G_GTAC'C Cuts at: 2693 2693 Size: 5368 MfeI C'AATT_G Cuts at: 2967 2967 Size: 5368 MluI A'CGCG_T Cuts at: 5244 5244 Size: 5368 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2381 4482 4666 1305 Size: 1076 2101 184 2007 Fragments arranged by size: 2101 2007 1076 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3546 Fragments arranged by size: 3546 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3326 3685 3844 518 Size: 648 2160 359 159 2042 Fragments arranged by size: 2160 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2143 2169 2784 3461 4609 4854 1606 Size: 537 26 615 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 615 537 245 26 NcoI C'CATG_G Cuts at: 513 1065 2226 513 Size: 552 1161 3655 Fragments arranged by size: 3655 1161 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5368 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5368 NotI GC'GGCC_GC Cuts at: 2815 2815 Size: 5368 NruI TCG'CGA Cuts at: 2617 2617 Size: 5368 NspI r_CATG'y Cuts at: 1132 1229 2726 4271 1132 Size: 97 1497 1545 2229 Fragments arranged by size: 2229 1545 1497 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5368 PciI A'CATG_T Cuts at: 4267 4267 Size: 5368 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5368 PfoI T'CCnGG_A Cuts at: 2393 2570 2393 Size: 177 5191 PmeI GTTT'AAAC Cuts at: 2672 2672 Size: 5368 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3437 3469 4950 4982 3437 Size: 32 1481 32 3823 Fragments arranged by size: 3823 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2556 2556 Size: 5368 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5368 PsiI TTA'TAA Cuts at: 2938 2938 Size: 5368 PstI C_TGCA'G Cuts at: 830 2720 830 Size: 1890 3478 PvuI CG_AT'CG Cuts at: 2001 2621 2001 Size: 620 4748 SacII CC_GC'GG Cuts at: 2144 2170 2144 Size: 26 5342 SalI G'TCGA_C Cuts at: 1936 2479 2710 1936 Size: 543 231 4594 Fragments arranged by size: 4594 543 231 SbfI CC_TGCA'GG Cuts at: 2720 2720 Size: 5368 ScaI AGT'ACT Cuts at: 3616 5328 3616 Size: 1712 3656 SfcI C'TryA_G Cuts at: 826 1045 2434 2716 3851 4532 4723 826 Size: 219 1389 282 1135 681 191 1471 Fragments arranged by size: 1471 1389 1135 681 282 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5368 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5368 SmaI CCC'GGG Cuts at: 1872 2695 1872 Size: 823 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2051 3472 4373 4635 820 Size: 197 926 108 1421 901 262 1553 Fragments arranged by size: 1553 1421 926 901 262 197 108 SnaBI TAC'GTA Cuts at: 493 3159 493 Size: 2666 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5368 SphI G_CATG'C Cuts at: 1132 2726 1132 Size: 1594 3774 SspI AAT'ATT Cuts at: 5 52 3292 5 Size: 47 3240 2081 Fragments arranged by size: 3240 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5368 StyI C'CwwG_G Cuts at: 513 1065 2157 2226 2369 2807 513 Size: 552 1092 69 143 438 3074 Fragments arranged by size: 3074 1092 552 438 143 69 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2442 2466 2518 2556 3395 3412 3565 Size: 1255 862 24 52 38 839 17 153 Cuts at: 3565 3750 4195 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 862 839 445 185 153 52 38 24 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2133 3614 5326 Size: 274 80 33 51 1443 156 1481 1712 Cuts at: 5326 96 Size: 138 Fragments arranged by size: 1712 1481 1443 274 156 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5328 5360 5328 Size: 32 5336 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5368 XbaI T'CTAG_A Cuts at: 2704 2704 Size: 5368 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5368 XmaI C'CCGG_G Cuts at: 1870 2693 1870 Size: 823 4545 XmnI GAAnn'nnTTC Cuts at: 3497 3497 Size: 5368 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3176 276 Size: 53 83 186 936 1642 2468 Fragments arranged by size: 2468 1642 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI