Restriction map for
pFN21AB4998
(Linear) (Six-base) MAPSORT of: KIBB4998.seq Check: 279 from: 1 to: 663
KIBB4998.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AccI GT'mk_AC
Cuts at: 0 475 663
Size: 475 188
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 659 663
Size: 659 4
AflIII A'CryG_T
Cuts at: 0 125 663
Size: 125 538
AvrII C'CTAG_G
Cuts at: 0 364 663
Size: 364 299
BbsI GAAGACnn'nnnn_
Cuts at: 0 90 663
Size: 90 573
BbvCI CC'TCA_GC
Cuts at: 0 88 203 663
Size: 88 115 460
Fragments arranged by size:
460 115 88
BglII A'GATC_T
Cuts at: 0 532 663
Size: 532 131
BmgBI CAC'GTC
Cuts at: 0 126 663
Size: 126 537
BmrI ACTGGGnnnn_n'
Cuts at: 0 574 663
Size: 574 89
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 513 663
Size: 513 150
Bpu10I CC'TnA_GC
Cuts at: 0 88 203 663
Size: 88 115 460
Fragments arranged by size:
460 115 88
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 663
Size: 31 632
BsaI GGTCTCn'nnnn_
Cuts at: 0 416 531 563 663
Size: 416 115 32 100
Fragments arranged by size:
416 115 100 32
BsaHI Gr'CG_yC
Cuts at: 0 348 372 663
Size: 348 24 291
Fragments arranged by size:
348 291 24
BsiEI CG_ry'CG
Cuts at: 0 12 246 279 475 616 663
Size: 12 234 33 196 141 47
Fragments arranged by size:
234 196 141 47 33 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 314 663
Size: 314 349
BsrBI CCG'CTC
Cuts at: 0 437 652 663
Size: 437 215 11
Fragments arranged by size:
437 215 11
BsrGI T'GTAC_A
Cuts at: 0 128 663
Size: 128 535
BssHII G'CGCG_C
Cuts at: 0 268 663
Size: 268 395
BstYI r'GATC_y
Cuts at: 0 490 532 663
Size: 490 42 131
Fragments arranged by size:
490 131 42
BtgI C'CryG_G
Cuts at: 0 136 162 221 663
Size: 136 26 59 442
Fragments arranged by size:
442 136 59 26
DrdI GACnn_nn'nnGTC
Cuts at: 0 483 663
Size: 483 180
EaeI y'GGCC_r
Cuts at: 0 9 243 276 663
Size: 9 234 33 387
Fragments arranged by size:
387 234 33 9
EagI C'GGCC_G
Cuts at: 0 9 243 276 663
Size: 9 234 33 387
Fragments arranged by size:
387 234 33 9
EarI CTCTTCn'nnn_
Cuts at: 0 613 635 663
Size: 613 22 28
Fragments arranged by size:
613 28 22
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 513 659 663
Size: 513 146 4
Fragments arranged by size:
513 146 4
EcoO109I rG'GnC_Cy
Cuts at: 0 551 663
Size: 551 112
HincII GTy'rAC
Cuts at: 0 476 663
Size: 476 187
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 663
Size: 376 287
MspA1I CmG'CkG
Cuts at: 0 138 164 663
Size: 138 26 499
Fragments arranged by size:
499 138 26
NcoI C'CATG_G
Cuts at: 0 221 663
Size: 221 442
NruI TCG'CGA
Cuts at: 0 612 663
Size: 612 51
PfoI T'CCnGG_A
Cuts at: 0 388 565 663
Size: 388 177 98
Fragments arranged by size:
388 177 98
PpuMI rG'GwC_Cy
Cuts at: 0 551 663
Size: 551 112
PvuI CG_AT'CG
Cuts at: 0 616 663
Size: 616 47
SacII CC_GC'GG
Cuts at: 0 139 165 663
Size: 139 26 498
Fragments arranged by size:
498 139 26
SalI G'TCGA_C
Cuts at: 0 474 663
Size: 474 189
SfcI C'TryA_G
Cuts at: 0 429 663
Size: 429 234
SmlI C'TyrA_G
Cuts at: 0 46 663
Size: 46 617
StyI C'CwwG_G
Cuts at: 0 152 221 364 663
Size: 152 69 143 299
Fragments arranged by size:
299 152 143 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 437 461 513 551 663
Size: 437 24 52 38 112
Fragments arranged by size:
437 112 52 38 24
TatI w'GTAC_w
Cuts at: 0 128 663
Size: 128 535
Enzymes that do cut:
AccI AcuI AflIII AvrII BbsI BbvCI BglII BmgBI
BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsiEI BspMI
BsrBI BsrGI BssHII BstYI BtgI DrdI EaeI EagI
EarI Eco57MI EcoO109I HincII MmeI MspA1I NcoI NruI
PfoI PpuMI PvuI SacII SalI SfcI SmlI StyI
TaqII TatI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BcgI BciVI BclI BfrBI BglI BlpI Bme1580I BmtI
BplI BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DraIII EciI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII
Hin4I HindIII HpaI KasI KpnI MfeI MluI MscI
MslI NaeI NarI NdeI NgoMIV NheI NotI NsiI
NspI PacI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PstI PvuII RsrII
SacI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI