(Circular) (Six-base) MAPSORT of: pFN21AB4907.seq Check: 1514 from: 1 to: 5911 pFN21AB4907 5911 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2279 3721 278 Size: 53 83 186 936 743 1442 2468 Fragments arranged by size: 2468 1442 936 743 186 83 53 AccI GT'mk_AC Cuts at: 1937 2592 3254 1937 Size: 655 662 4594 Fragments arranged by size: 4594 662 655 Acc65I G'GTAC_C Cuts at: 3232 3232 Size: 5911 AclI AA'CG_TT Cuts at: 1563 4038 4411 1563 Size: 2475 373 3063 Fragments arranged by size: 3063 2475 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2391 3026 3974 5358 5626 1632 Size: 759 635 948 1384 268 1917 Fragments arranged by size: 1917 1384 948 759 635 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5714 AflIII A'CryG_T Cuts at: 1203 4810 5787 1203 Size: 3607 977 1327 Fragments arranged by size: 3607 1327 977 AgeI A'CCGG_T Cuts at: 4742 4742 Size: 5911 AhdI GACnn_n'nnGTC Cuts at: 4640 4640 Size: 5911 AleI CACnn'nnGTG Cuts at: 1166 2958 1166 Size: 1792 4119 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5911 AlwNI CAG_nnn'CTG Cuts at: 2321 3654 5226 2321 Size: 1333 1572 3006 Fragments arranged by size: 3006 1572 1333 ApaI G_GGCC'C Cuts at: 2445 2445 Size: 5911 ApaLI G'TGCA_C Cuts at: 3968 5124 3968 Size: 1156 4755 ApoI r'AATT_y Cuts at: 1493 2524 3220 3446 3598 5707 1493 Size: 1031 696 226 152 2109 1697 Fragments arranged by size: 2109 1697 1031 696 226 152 AseI AT'TA_AT Cuts at: 160 1032 4465 160 Size: 872 3433 1606 Fragments arranged by size: 3433 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5911 AvaI C'yCGr_G Cuts at: 1870 1943 2688 3236 1870 Size: 73 745 548 4545 Fragments arranged by size: 4545 745 548 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2385 2418 2804 2837 3034 3067 2385 Size: 33 386 33 197 33 5229 Fragments arranged by size: 5229 386 197 33 33 33 BamHI G'GATC_C Cuts at: 3241 3608 3241 Size: 367 5544 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2581 3232 4687 618 Size: 325 217 627 794 651 1455 1842 Fragments arranged by size: 1842 1455 794 651 627 325 217 BanII G_rGCy'C Cuts at: 1391 2199 2445 3230 1391 Size: 808 246 785 4072 Fragments arranged by size: 4072 808 785 246 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2352 928 Size: 971 453 4487 Fragments arranged by size: 4487 971 453 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4102 4136 1903 Size: 34 2165 34 3678 Fragments arranged by size: 3678 2165 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2960 3803 5013 2960 Size: 843 1210 3858 Fragments arranged by size: 3858 1210 843 BclI T'GATC_A Cuts at: 1548 1548 Size: 5911 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4522 136 Size: 107 122 71 631 605 2850 1525 Fragments arranged by size: 2850 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5906 5906 Size: 5911 BlpI GC'TnA_GC Cuts at: 3328 3328 Size: 5911 Bme1580I G_kGCm'C Cuts at: 1790 2445 3972 5128 1790 Size: 655 1527 1156 2573 Fragments arranged by size: 2573 1527 1156 655 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5911 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2297 2819 3642 Size: 416 53 83 399 24 880 522 823 Cuts at: 3642 4600 442 Size: 958 1753 Fragments arranged by size: 1753 958 880 823 522 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5911 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2421 2920 4571 1239 Size: 1182 499 1651 2579 Fragments arranged by size: 2579 1651 1182 499 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4036 4901 5199 4036 Size: 865 298 4748 Fragments arranged by size: 4748 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2133 4574 882 Size: 617 634 2441 2219 Fragments arranged by size: 2441 2219 634 617 BsaAI yAC'GTr Cuts at: 493 1204 3702 493 Size: 711 2498 2702 Fragments arranged by size: 2702 2498 711 BsaBI GATnn'nnATC Cuts at: 5905 5905 Size: 5911 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2276 2908 3718 4100 Size: 53 83 186 936 743 632 810 382 Cuts at: 4100 4735 275 Size: 635 1451 Fragments arranged by size: 1451 936 810 743 635 632 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3015 4344 4742 5016 5163 5574 3015 Size: 1329 398 274 147 411 3352 Fragments arranged by size: 3352 1329 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2195 2225 1178 Size: 30 165 30 792 30 4864 Fragments arranged by size: 4864 792 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2317 1186 Size: 1131 4780 BseYI C'CCAG_C Cuts at: 5114 5610 5114 Size: 496 5415 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3049 3170 1163 Size: 573 1313 121 3904 Fragments arranged by size: 3904 1313 573 121 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3361 4122 5150 1540 Size: 275 186 1360 761 1028 2301 Fragments arranged by size: 2301 1360 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2903 3972 4057 5128 2903 Size: 1069 85 1071 3686 Fragments arranged by size: 3686 1071 1069 85 BsmI GAATG_Cn' Cuts at: 3422 3515 3422 Size: 93 5818 BsmBI CGTCTCn'nnnn_ Cuts at: 2908 2908 Size: 5911 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2199 2445 2903 3230 3972 4057 5128 Size: 399 409 246 458 327 742 85 1071 Cuts at: 5128 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 458 409 399 327 246 85 BspEI T'CCGG_A Cuts at: 5574 5574 Size: 5911 BspHI T'CATG_A Cuts at: 3800 5530 3800 Size: 1730 4181 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2745 3266 844 Size: 1901 521 3489 Fragments arranged by size: 3489 1901 521 BsrBI CCG'CTC Cuts at: 1394 1823 3798 1394 Size: 429 1975 3507 Fragments arranged by size: 3507 1975 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4406 4580 65 Size: 1183 3158 174 1396 Fragments arranged by size: 3158 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2083 4555 4742 1700 Size: 110 273 2472 187 2869 Fragments arranged by size: 2869 2472 273 187 110 BsrGI T'GTAC_A Cuts at: 96 2985 96 Size: 2889 3022 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5911 BssSI C'ACGA_G Cuts at: 3971 4983 3971 Size: 1012 4899 BstAPI GCAn_nnn'nTGC Cuts at: 794 2485 794 Size: 1691 4220 BstBI TT'CG_AA Cuts at: 4715 4715 Size: 5911 BstEII G'GTnAC_C Cuts at: 1184 2607 1184 Size: 1423 4488 BstYI r'GATC_y Cuts at: 1972 2774 3241 3608 3993 4010 5462 5548 5560 Size: 802 467 367 385 17 1452 86 12 Cuts at: 5560 5906 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 802 467 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2565 2665 513 Size: 552 1500 100 3759 Fragments arranged by size: 3759 1500 552 100 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5172 Fragments arranged by size: 5172 683 56 BtsI GCAGTG_nn' Cuts at: 3427 4221 4241 3427 Size: 794 20 5097 Fragments arranged by size: 5097 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5911 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5876 DraI TTT'AAA Cuts at: 3215 3215 Size: 5911 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4918 809 Size: 507 3602 1802 Fragments arranged by size: 3602 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3358 4247 5876 Size: 54 1425 50 275 18 1528 889 1629 Cuts at: 5876 8 Size: 43 Fragments arranged by size: 1629 1528 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3358 1537 Size: 275 1546 4090 Fragments arranged by size: 4090 1546 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2015 2356 3841 1353 Size: 662 341 1485 3423 Fragments arranged by size: 3423 1485 662 341 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4498 4872 5018 387 Size: 1064 138 2909 374 146 1280 Fragments arranged by size: 2909 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2391 2421 2920 3026 3974 4571 5358 Size: 393 759 30 499 106 948 597 787 Cuts at: 5358 5626 1239 Size: 268 1524 Fragments arranged by size: 1524 948 787 759 597 499 393 268 106 30 EcoO109I rG'GnC_Cy Cuts at: 2441 2442 2441 Size: 1 5910 EcoRI G'AATT_C Cuts at: 3220 3598 3220 Size: 378 5533 FspI TGC'GCA Cuts at: 4417 4417 Size: 5911 HaeII r_GCGC'y Cuts at: 1678 5058 1678 Size: 3380 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2198 2230 4552 4584 4626 Size: 32 135 32 626 32 2322 32 42 Cuts at: 4626 4658 1373 Size: 32 2626 Fragments arranged by size: 2626 2322 626 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2424 3255 3501 669 Size: 988 281 486 831 246 3079 Fragments arranged by size: 3079 988 831 486 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5911 HpaI GTT'AAC Cuts at: 3501 3501 Size: 5911 KpnI G_GTAC'C Cuts at: 3236 3236 Size: 5911 MfeI C'AATT_G Cuts at: 3510 3510 Size: 5911 MluI A'CGCG_T Cuts at: 5787 5787 Size: 5911 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2449 5025 5209 1305 Size: 1144 2576 184 2007 Fragments arranged by size: 2576 2007 1144 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4089 Fragments arranged by size: 4089 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2564 2958 3136 3869 4228 4387 518 Size: 648 1398 394 178 733 359 159 2042 Fragments arranged by size: 2042 1398 733 648 394 359 178 159 MspA1I CmG'CkG Cuts at: 1606 2600 3327 4004 5152 5397 1606 Size: 994 727 677 1148 245 2120 Fragments arranged by size: 2120 1148 994 727 677 245 NaeI GCC'GGC Cuts at: 2085 2085 Size: 5911 NcoI C'CATG_G Cuts at: 513 1065 2665 513 Size: 552 1600 3759 Fragments arranged by size: 3759 1600 552 NdeI CA'TA_TG Cuts at: 387 2016 3201 387 Size: 1629 1185 3097 Fragments arranged by size: 3097 1629 1185 NgoMIV G'CCGG_C Cuts at: 2083 2083 Size: 5911 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5911 NotI GC'GGCC_GC Cuts at: 3358 3358 Size: 5911 NspI r_CATG'y Cuts at: 1132 1229 3269 4814 1132 Size: 97 2040 1545 2229 Fragments arranged by size: 2229 2040 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5911 PciI A'CATG_T Cuts at: 4810 4810 Size: 5911 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5911 PmeI GTTT'AAAC Cuts at: 3215 3215 Size: 5911 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3980 4012 5493 5525 3980 Size: 32 1481 32 4366 Fragments arranged by size: 4366 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5911 PsiI TTA'TAA Cuts at: 3481 3481 Size: 5911 PspOMI G'GGCC_C Cuts at: 2441 2441 Size: 5911 PstI C_TGCA'G Cuts at: 830 3263 830 Size: 2433 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5911 PvuII CAG'CTG Cuts at: 2600 2600 Size: 5911 SalI G'TCGA_C Cuts at: 1936 3253 1936 Size: 1317 4594 SbfI CC_TGCA'GG Cuts at: 3263 3263 Size: 5911 ScaI AGT'ACT Cuts at: 4159 5871 4159 Size: 1712 4199 SfcI C'TryA_G Cuts at: 826 1045 2217 3259 4394 5075 5266 826 Size: 219 1172 1042 1135 681 191 1471 Fragments arranged by size: 1471 1172 1135 1042 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5911 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5911 SmaI CCC'GGG Cuts at: 1872 3238 1872 Size: 1366 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4015 4916 5178 820 Size: 197 926 2072 901 262 1553 Fragments arranged by size: 2072 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3702 493 Size: 3209 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5911 SphI G_CATG'C Cuts at: 1132 3269 1132 Size: 2137 3774 SspI AAT'ATT Cuts at: 5 52 3835 5 Size: 47 3783 2081 Fragments arranged by size: 3783 2081 47 StuI AGG'CCT Cuts at: 1517 2694 1517 Size: 1177 4734 StyI C'CwwG_G Cuts at: 513 1065 2665 3350 513 Size: 552 1600 685 3074 Fragments arranged by size: 3074 1600 685 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3938 3955 4108 4293 4738 325 Size: 1255 2358 17 153 185 445 1498 Fragments arranged by size: 2358 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2803 2985 4157 Size: 274 80 33 51 1443 826 182 1172 Cuts at: 4157 5869 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1172 826 274 182 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5871 5903 5871 Size: 32 5879 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5911 XbaI T'CTAG_A Cuts at: 3247 3247 Size: 5911 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5911 XmaI C'CCGG_G Cuts at: 1870 3236 1870 Size: 1366 4545 XmnI GAAnn'nnTTC Cuts at: 2163 3131 4040 2163 Size: 968 909 4034 Fragments arranged by size: 4034 968 909 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2277 3719 276 Size: 53 83 186 936 743 1442 2468 Fragments arranged by size: 2468 1442 936 743 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI