Restriction map for
pFN21AB4875
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# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII AccI AclI AcsI AflII
AgeI AhdI Alw44I ApaLI ApoI
AseI AsnI AspEI AspI AvrII
BamHI BbsI BciVI BclI BfrI
BglII BlnI BpuAI BsaBI BsiWI
BsmI BspDI BspHI BspLU11I BsrDI
Bst1107I BstBI BstZ17I ClaI CpoI
DraI Eam1105I EcoRI EcoRV EcoT22I
FbaI HincII HindII HindIII HpaI
MamI MluI MseI NdeI NheI
NotI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI Tru9I Tth111I
XbaI XhoI
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# ONE CUT ENZYMES ( 26 enzymes) #
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Acc65I 948 GGTACC G^GTACC
AocI 2875 CCTAAGG CC^T[ACGT]AGG
Asp700I 687 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 948 GGTACC G^GTACC
AviII 152 TGCGCA TGC^GCA
BbvCI 2691 GCTGAGG CCTCAGC(-5/-2)
BsmBI 2152 GAGACG CGTCTC(1/5)
BssSI 1204 CACGAG CACGAG(-5/-1)
Bsu36I 2875 CCTAAGG CC^T[ACGT]AGG
DraIII 815 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1632 GACCACTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2875 CCTAAGG CC^T[ACGT]AGG
FspI 152 TGCGCA TGC^GCA
KpnI 948 GGTACC GGTAC^C
KspI 1956 CCGCGG CCGC^GG
MfeI 1890 CAATTG C^AATTG
MunI 1890 CAATTG C^AATTG
NcoI 1577 CCATGG C^CATGG
NruI 1902 TCGCGA TCG^CGA
SacI 502 GAGCTC GAGCT^C
SacII 1956 CCGCGG CCGC^GG
SapI 261 GCTCTTC GCTCTTC(1/4)
SmlI 331 CTTGAG C^T[CT][GA]AG
StuI 525 AGGCCT AGG^CCT
Tsp509I 1891 AATT ^AATT
XmnI 687 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
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AccIII 809 TCCGGA T^CCGGA
1268 TCCGGA
AflIII 1220 ACGTGT A^C[GA][CT]GT
1367 ACGTGT
Aor51HI 1530 AGCGCT AGC^GCT
2135 AGCGCT
AscI 2019 GGCGCGCC GG^CGCGCC
2175 GGCGCGCC
BcgI 1967 CGATCACGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2223 CGAGGCCGCTGC
BsaWI 809 TCCGGA [AT]^CCGG[AT]
1268 TCCGGA
BseAI 809 TCCGGA T^CCGGA
1268 TCCGGA
Bsp1407I 1049 TGTACA T^GTACA
2321 TGTACA
BspEI 809 TCCGGA T^CCGGA
1268 TCCGGA
BsrGI 1049 TGTACA T^GTACA
2321 TGTACA
BstEII 1629 GGTGACC G^GT[ACGT]ACC
2391 GGTGACC
BstPI 1629 GGTGACC G^GT[ACGT]ACC
2391 GGTGACC
BstXI 1311 CCAGGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3092 CCAAGCAGCTGG
BstYI 2364 AGATCC [GA]^GATC[CT]
2466 GGATCT
EarI 262 CTCTTC CTCTTC(1/4)
226 GAAGAG
Eco47III 1530 AGCGCT AGC^GCT
2135 AGCGCT
EcoO65I 1629 GGTGACC G^GT[ACGT]ACC
2391 GGTGACC
FseI 1551 GGCCGGCC GGCCGG^CC
2293 GGCCGGCC
Ksp632I 262 CTCTTC CTCTTC(1/4)
226 GAAGAG
MflI 2364 AGATCC [GA]^GATC[CT]
2466 GGATCT
MroI 809 TCCGGA T^CCGGA
1268 TCCGGA
SmaI 1765 CCCGGG CCC^GGG
2170 CCCGGG
SspBI 1049 TGTACA T^GTACA
2321 TGTACA
TfiI 1162 GATTC G^A[AT]TC
2117 GATTC
XhoII 2364 AGATCC [GA]^GATC[CT]
2466 GGATCT
XmaCI 1765 CCCGGG C^CCGGG
2170 CCCGGG
XmaI 1765 CCCGGG C^CCGGG
2170 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbrPI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssHII BssKI BstAPI
BstF5I BstNI BstUI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EclXI
Eco52I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV PflMI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
StyI TaqI TseI Tsp45I TspRI
TthHB8I Van91I XcmI