(Circular) (Six-base) MAPSORT of: pFN21AB4875.seq Check: 2136 from: 1 to: 7831 pFN21AB4875 7831 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5641 278 Size: 53 83 186 936 4105 2468 Fragments arranged by size: 4105 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5174 1937 Size: 3237 4594 Acc65I G'GTAC_C Cuts at: 2953 5152 2953 Size: 2199 5632 AclI AA'CG_TT Cuts at: 1563 5958 6331 1563 Size: 4395 373 3063 Fragments arranged by size: 4395 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2430 5894 7278 7546 1632 Size: 798 3464 1384 268 1917 Fragments arranged by size: 3464 1917 1384 798 268 AfeI AGC'GCT Cuts at: 3537 4142 3537 Size: 605 7226 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7634 AflIII A'CryG_T Cuts at: 1203 3225 3372 6730 7707 1203 Size: 2022 147 3358 977 1327 Fragments arranged by size: 3358 2022 1327 977 147 AgeI A'CCGG_T Cuts at: 6662 6662 Size: 7831 AhdI GACnn_n'nnGTC Cuts at: 6560 6560 Size: 7831 AleI CACnn'nnGTG Cuts at: 1166 3748 4133 1166 Size: 2582 385 4864 Fragments arranged by size: 4864 2582 385 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3839 1184 Size: 2655 5176 AlwNI CAG_nnn'CTG Cuts at: 2318 3610 3943 4210 4246 4373 4426 4466 4916 Size: 1292 333 267 36 127 53 40 450 Cuts at: 4916 5574 7146 2318 Size: 658 1572 3003 Fragments arranged by size: 3003 1572 1292 658 450 333 267 127 53 40 36 ApaI G_GGCC'C Cuts at: 2150 2723 2862 3148 3716 4366 2150 Size: 573 139 286 568 650 5615 Fragments arranged by size: 5615 650 573 568 286 139 ApaLI G'TGCA_C Cuts at: 5888 7044 5888 Size: 1156 6675 ApoI r'AATT_y Cuts at: 1493 5140 5366 5518 7627 1493 Size: 3647 226 152 2109 1697 Fragments arranged by size: 3647 2109 1697 226 152 AscI GG'CGCG_CC Cuts at: 4025 4181 4025 Size: 156 7675 AseI AT'TA_AT Cuts at: 160 1032 6385 160 Size: 872 5353 1606 Fragments arranged by size: 5353 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7831 AvaI C'yCGr_G Cuts at: 1870 1943 2510 3708 3770 4175 4226 4845 5156 Size: 73 567 1198 62 405 51 619 311 Cuts at: 5156 1870 Size: 4545 Fragments arranged by size: 4545 1198 619 567 405 311 73 62 51 BamHI G'GATC_C Cuts at: 5161 5528 5161 Size: 367 7464 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2488 2771 2828 2902 2953 Size: 325 217 627 701 283 57 74 51 Cuts at: 2953 3693 3719 3766 3823 3868 4255 4687 5152 Size: 740 26 47 57 45 387 432 465 Cuts at: 5152 6607 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 740 701 627 465 432 387 325 283 217 74 57 57 51 47 45 26 BanII G_rGCy'C Cuts at: 1391 2036 2150 2392 2511 2723 2862 2918 2939 Size: 645 114 242 119 212 139 56 21 Cuts at: 2939 3041 3148 3716 3743 3796 4366 5111 5150 Size: 102 107 568 27 53 570 745 39 Cuts at: 5150 1391 Size: 4072 Fragments arranged by size: 4072 745 645 570 568 242 212 139 119 114 107 102 56 53 39 27 21 BbeI G_GCGC'C Cuts at: 2906 3697 3723 3770 3872 4259 4691 2906 Size: 791 26 47 102 387 432 6046 Fragments arranged by size: 6046 791 432 387 102 47 26 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6860 BbvCI CC'TCA_GC Cuts at: 4697 4697 Size: 7831 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3961 3995 4217 4251 6022 Size: 34 50 34 1940 34 222 34 1771 Cuts at: 6022 6056 1903 Size: 34 3678 Fragments arranged by size: 3678 1940 1771 222 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5723 6933 5723 Size: 1210 6621 BclI T'GATC_A Cuts at: 1548 1548 Size: 7831 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2101 2857 3030 Size: 107 122 71 631 605 429 756 173 Cuts at: 3030 3288 3791 3813 3837 4098 4160 4354 5126 Size: 258 503 22 24 261 62 194 772 Cuts at: 5126 6442 136 Size: 1316 1525 Fragments arranged by size: 1525 1316 772 756 631 605 503 429 261 258 194 173 122 107 71 62 24 22 BglII A'GATC_T Cuts at: 7826 7826 Size: 7831 BlpI GC'TnA_GC Cuts at: 2912 2938 2990 4905 5248 2912 Size: 26 52 1915 343 5495 Fragments arranged by size: 5495 1915 343 52 26 Bme1580I G_kGCm'C Cuts at: 1790 2150 2215 2221 2491 2562 2723 2774 2862 Size: 360 65 6 270 71 161 51 88 Cuts at: 2862 3148 3394 3716 3828 4366 5892 7048 1790 Size: 286 246 322 112 538 1526 1156 2573 Fragments arranged by size: 2573 1526 1156 538 360 322 286 270 246 161 112 88 71 65 51 6 BmgBI CAC'GTC Cuts at: 1318 2365 3913 1318 Size: 1047 1548 5236 Fragments arranged by size: 5236 1548 1047 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4544 4605 4653 Size: 416 53 83 399 24 3127 61 48 Cuts at: 4653 5562 6520 442 Size: 909 958 1753 Fragments arranged by size: 3127 1753 958 909 416 399 83 61 53 48 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7831 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2446 4828 4954 2446 Size: 2382 126 5323 Fragments arranged by size: 5323 2382 126 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2615 2651 2912 4443 4799 4853 5030 6491 Size: 1376 36 261 1531 356 54 177 1461 Cuts at: 6491 1239 Size: 2579 Fragments arranged by size: 2579 1531 1461 1376 356 261 177 54 36 Bpu10I CC'TnA_GC Cuts at: 3795 4697 4958 3795 Size: 902 261 6668 Fragments arranged by size: 6668 902 261 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2357 5956 6821 7119 2357 Size: 3599 865 298 3069 Fragments arranged by size: 3599 3069 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2403 4049 4869 4942 6494 882 Size: 617 904 1646 820 73 1552 2219 Fragments arranged by size: 2219 1646 1552 904 820 617 73 BsaAI yAC'GTr Cuts at: 493 1204 2899 3226 3373 4135 4718 5622 493 Size: 711 1695 327 147 762 583 904 2702 Fragments arranged by size: 2702 1695 904 762 711 583 327 147 BsaBI GATnn'nnATC Cuts at: 7825 7825 Size: 7831 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2903 3694 3720 3767 Size: 53 83 186 936 1370 791 26 47 Cuts at: 3767 3869 4113 4256 4688 5638 6020 6655 275 Size: 102 244 143 432 950 382 635 1451 Fragments arranged by size: 1451 1370 950 936 791 635 432 382 244 186 143 102 83 53 47 26 BsaWI w'CCGG_w Cuts at: 2814 3273 6264 6662 6936 7083 7494 2814 Size: 459 2991 398 274 147 411 3151 Fragments arranged by size: 3151 2991 459 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3038 3068 4772 4802 4826 Size: 30 165 30 1635 30 1704 30 24 Cuts at: 4826 4856 5070 5100 1178 Size: 30 214 30 3909 Fragments arranged by size: 3909 1704 1635 214 165 30 30 30 30 30 30 24 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2612 2762 3059 3062 3260 4496 4568 5027 Size: 1426 150 297 3 198 1236 72 459 Cuts at: 5027 5030 5075 1186 Size: 3 45 3942 Fragments arranged by size: 3942 1426 1236 459 297 198 150 72 45 3 3 BseYI C'CCAG_C Cuts at: 2290 2716 2918 3103 3147 3835 4214 4239 5104 Size: 426 202 185 44 688 379 25 865 Cuts at: 5104 7034 7530 2290 Size: 1930 496 2591 Fragments arranged by size: 2591 1930 865 688 496 426 379 202 185 44 25 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2550 2762 3388 4397 4412 4484 5091 Size: 573 814 212 626 1009 15 72 607 Cuts at: 5091 1163 Size: 3903 Fragments arranged by size: 3903 1009 814 626 607 573 212 72 15 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2583 3558 3849 3883 3961 4300 Size: 275 186 582 975 291 34 78 339 Cuts at: 4300 5281 6042 7070 1540 Size: 981 761 1028 2301 Fragments arranged by size: 2301 1028 981 975 761 582 339 291 275 186 78 34 BsiHKAI G_wGCw'C Cuts at: 2511 2637 2898 5892 5977 7048 2511 Size: 126 261 2994 85 1071 3294 Fragments arranged by size: 3294 2994 1071 261 126 85 BsmI GAATG_Cn' Cuts at: 5342 5435 5342 Size: 93 7738 BsmBI CGTCTCn'nnnn_ Cuts at: 4151 4151 Size: 7831 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2036 2150 2215 2221 2392 2491 2511 Size: 399 246 114 65 6 171 99 20 Cuts at: 2511 2562 2637 2723 2774 2862 2898 2918 2939 Size: 51 75 86 51 88 36 20 21 Cuts at: 2939 3041 3148 3394 3716 3743 3796 3828 4366 Size: 102 107 246 322 27 53 32 538 Cuts at: 4366 5111 5150 5892 5977 7048 1391 Size: 745 39 742 85 1071 2174 Fragments arranged by size: 2174 1071 745 742 538 399 322 246 246 171 114 107 102 99 88 86 85 75 65 53 51 51 39 36 32 27 21 20 20 6 BspEI T'CCGG_A Cuts at: 2814 3273 7494 2814 Size: 459 4221 3151 Fragments arranged by size: 4221 3151 459 BspHI T'CATG_A Cuts at: 5720 7450 5720 Size: 1730 6101 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2254 2484 3618 4986 5186 844 Size: 1410 230 1134 1368 200 3489 Fragments arranged by size: 3489 1410 1368 1134 230 200 BsrBI CCG'CTC Cuts at: 1394 1823 3357 3804 4296 5718 1394 Size: 429 1534 447 492 1422 3507 Fragments arranged by size: 3507 1534 1422 492 447 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6326 6500 65 Size: 1183 5078 174 1396 Fragments arranged by size: 5078 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2018 3076 3557 4299 4353 5043 6475 Size: 110 208 1058 481 742 54 690 1432 Cuts at: 6475 6662 1700 Size: 187 2869 Fragments arranged by size: 2869 1432 1058 742 690 481 208 187 110 54 BsrGI T'GTAC_A Cuts at: 96 3054 4326 96 Size: 2958 1272 3601 Fragments arranged by size: 3601 2958 1272 BssHII G'CGCG_C Cuts at: 1925 3566 3760 4025 4181 1925 Size: 1641 194 265 156 5575 Fragments arranged by size: 5575 1641 265 194 156 BssSI C'ACGA_G Cuts at: 3209 5891 6903 3209 Size: 2682 1012 4137 Fragments arranged by size: 4137 2682 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2374 3610 4426 794 Size: 1580 1236 816 4199 Fragments arranged by size: 4199 1580 1236 816 BstBI TT'CG_AA Cuts at: 6635 6635 Size: 7831 BstEII G'GTnAC_C Cuts at: 1184 3634 4396 1184 Size: 2450 762 4619 Fragments arranged by size: 4619 2450 762 BstXI CCAn_nnnn'nTGG Cuts at: 3323 5104 3323 Size: 1781 6050 BstYI r'GATC_y Cuts at: 1972 4369 4471 5161 5528 5913 5930 7382 7468 Size: 2397 102 690 367 385 17 1452 86 Cuts at: 7468 7480 7826 1972 Size: 12 346 1977 Fragments arranged by size: 2397 1977 1452 690 385 367 346 102 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4881 4881 Size: 7831 BtgI C'CryG_G Cuts at: 513 1065 2824 2864 2957 3582 3955 3961 4008 Size: 552 1759 40 93 625 373 6 47 Cuts at: 4008 4045 513 Size: 37 4299 Fragments arranged by size: 4299 1759 625 552 373 93 47 40 37 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2184 3123 487 Size: 683 56 958 939 5195 Fragments arranged by size: 5195 958 939 683 56 BtsI GCAGTG_nn' Cuts at: 2285 2567 2736 4774 5347 6141 6161 2285 Size: 282 169 2038 573 794 20 3955 Fragments arranged by size: 3955 2038 794 573 282 169 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7831 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4704 4739 529 Size: 35 4140 35 3621 Fragments arranged by size: 4140 3621 35 35 DraI TTT'AAA Cuts at: 5135 5135 Size: 7831 DraIII CAC_nnn'GTG Cuts at: 2825 2825 Size: 7831 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3643 6838 809 Size: 507 2327 3195 1802 Fragments arranged by size: 3195 2327 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2580 3429 3528 Size: 54 1425 50 275 18 750 849 99 Cuts at: 3528 3555 3559 3585 3621 3846 3880 3958 4000 Size: 27 4 26 36 225 34 78 42 Cuts at: 4000 4201 4297 4476 4584 5127 5278 6167 7796 Size: 201 96 179 108 543 151 889 1629 Cuts at: 7796 8 Size: 43 Fragments arranged by size: 1629 1425 889 849 750 543 275 225 201 179 151 108 99 96 78 54 50 43 42 36 34 27 26 18 4 EagI C'GGCC_G Cuts at: 1537 1812 2580 3555 3846 3880 3958 4297 5278 Size: 275 768 975 291 34 78 339 981 Cuts at: 5278 1537 Size: 4090 Fragments arranged by size: 4090 981 975 768 339 291 275 78 34 EarI CTCTTCn'nnn_ Cuts at: 1353 2226 2273 5761 1353 Size: 873 47 3488 3423 Fragments arranged by size: 3488 3423 873 47 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3498 5009 5072 6418 6792 6938 Size: 1064 138 1909 1511 63 1346 374 146 Cuts at: 6938 387 Size: 1280 Fragments arranged by size: 1909 1511 1346 1280 1064 374 146 138 63 EcoICRI GAG'CTC Cuts at: 2509 2509 Size: 7831 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2430 2615 2651 2912 4443 4799 4853 Size: 393 798 185 36 261 1531 356 54 Cuts at: 4853 5030 5894 6491 7278 7546 1239 Size: 177 864 597 787 268 1524 Fragments arranged by size: 1531 1524 864 798 787 597 393 356 268 261 185 177 54 36 EcoNI CCTnn'n_nnAGG Cuts at: 4637 4982 5012 4637 Size: 345 30 7456 Fragments arranged by size: 7456 345 30 EcoO109I rG'GnC_Cy Cuts at: 2103 2146 2187 2436 2858 2859 3488 3712 3993 Size: 43 41 249 422 1 629 224 281 Cuts at: 3993 4362 4363 4463 4546 4701 4773 4975 2103 Size: 369 1 100 83 155 72 202 4959 Fragments arranged by size: 4959 629 422 369 281 249 224 202 155 100 83 72 43 41 1 1 EcoRI G'AATT_C Cuts at: 5140 5518 5140 Size: 378 7453 FseI GG_CCGG'CC Cuts at: 3561 4303 3561 Size: 742 7089 FspI TGC'GCA Cuts at: 2159 6337 2159 Size: 4178 3653 HaeII r_GCGC'y Cuts at: 1678 2906 3283 3539 3697 3723 3770 3872 4073 Size: 1228 377 256 158 26 47 102 201 Cuts at: 4073 4144 4259 4691 6978 1678 Size: 71 115 432 2287 2531 Fragments arranged by size: 2531 2287 1228 432 377 256 201 158 115 102 71 47 26 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4826 4845 4858 4877 6472 Size: 32 135 32 3254 19 13 19 1595 Cuts at: 6472 6504 6546 6578 1373 Size: 32 42 32 2626 Fragments arranged by size: 3254 2626 1595 135 42 32 32 32 32 19 19 13 HincII GTy'rAC Cuts at: 669 1657 1938 5175 5421 669 Size: 988 281 3237 246 3079 Fragments arranged by size: 3237 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7831 HpaI GTT'AAC Cuts at: 5421 5421 Size: 7831 KasI G'GCGC_C Cuts at: 2902 3693 3719 3766 3868 4255 4687 2902 Size: 791 26 47 102 387 432 6046 Fragments arranged by size: 6046 791 432 387 102 47 26 KpnI G_GTAC'C Cuts at: 2957 5156 2957 Size: 2199 5632 MfeI C'AATT_G Cuts at: 3895 5430 3895 Size: 1535 6296 MluI A'CGCG_T Cuts at: 7707 7707 Size: 7831 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3022 4518 4737 6945 7129 1305 Size: 1717 1496 219 2208 184 2007 Fragments arranged by size: 2208 2007 1717 1496 219 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3530 3587 3623 4586 10 Size: 54 1425 343 1698 57 36 963 3255 Fragments arranged by size: 3255 1698 1425 963 343 57 54 36 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3748 3821 4133 5789 6148 6307 Size: 648 843 1739 73 312 1656 359 159 Cuts at: 6307 518 Size: 2042 Fragments arranged by size: 2042 1739 1656 843 648 359 312 159 73 MspA1I CmG'CkG Cuts at: 1606 2110 2163 2281 2763 2911 2922 3737 3943 Size: 504 53 118 482 148 11 815 206 Cuts at: 3943 3963 4036 4235 4423 5104 5247 5924 7072 Size: 20 73 199 188 681 143 677 1148 Cuts at: 7072 7317 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 815 681 677 504 482 245 206 199 188 148 143 118 73 53 20 11 NaeI GCC'GGC Cuts at: 2020 3559 4301 4355 2020 Size: 1539 742 54 5496 Fragments arranged by size: 5496 1539 742 54 NarI GG'CG_CC Cuts at: 2903 3694 3720 3767 3869 4256 4688 2903 Size: 791 26 47 102 387 432 6046 Fragments arranged by size: 6046 791 432 387 102 47 26 NcoI C'CATG_G Cuts at: 513 1065 3582 513 Size: 552 2517 4762 Fragments arranged by size: 4762 2517 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7831 NgoMIV G'CCGG_C Cuts at: 2018 3557 4299 4353 2018 Size: 1539 742 54 5496 Fragments arranged by size: 5496 1539 742 54 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7831 NotI GC'GGCC_GC Cuts at: 5278 5278 Size: 7831 NruI TCG'CGA Cuts at: 3909 3909 Size: 7831 NspI r_CATG'y Cuts at: 1132 1229 5189 6734 1132 Size: 97 3960 1545 2229 Fragments arranged by size: 3960 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3682 1348 Size: 2334 5497 PciI A'CATG_T Cuts at: 6730 6730 Size: 7831 PflMI CCAn_nnn'nTGG Cuts at: 1108 3583 4009 4466 4871 4916 1108 Size: 2475 426 457 405 45 4023 Fragments arranged by size: 4023 2475 457 426 405 45 PfoI T'CCnGG_A Cuts at: 2592 3315 2592 Size: 723 7108 PmeI GTTT'AAAC Cuts at: 5135 5135 Size: 7831 PmlI CAC'GTG Cuts at: 2899 3226 3373 4135 2899 Size: 327 147 762 6595 Fragments arranged by size: 6595 762 327 147 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2600 2632 3203 3235 5900 5932 7413 7445 2600 Size: 32 571 32 2665 32 1481 32 2986 Fragments arranged by size: 2986 2665 1481 571 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2436 3993 4773 4975 2436 Size: 1557 780 202 5292 Fragments arranged by size: 5292 1557 780 202 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7831 PsiI TTA'TAA Cuts at: 5401 5401 Size: 7831 PspOMI G'GGCC_C Cuts at: 2146 2719 2858 3144 3712 4362 2146 Size: 573 139 286 568 650 5615 Fragments arranged by size: 5615 650 573 568 286 139 PstI C_TGCA'G Cuts at: 830 2251 3732 4035 4344 4971 4983 5055 5183 Size: 1421 1481 303 309 627 12 72 128 Cuts at: 5183 830 Size: 3478 Fragments arranged by size: 3478 1481 1421 627 309 303 128 72 12 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7831 PvuII CAG'CTG Cuts at: 2911 2922 3943 4036 4423 5104 2911 Size: 11 1021 93 387 681 5638 Fragments arranged by size: 5638 1021 681 387 93 11 SacI G_AGCT'C Cuts at: 2511 2511 Size: 7831 SacII CC_GC'GG Cuts at: 3964 3964 Size: 7831 SalI G'TCGA_C Cuts at: 1936 5173 1936 Size: 3237 4594 SanDI GG'GwC_CC Cuts at: 2436 3993 4773 2436 Size: 1557 780 5494 Fragments arranged by size: 5494 1557 780 SapI GCTCTTCn'nnn_ Cuts at: 2273 2273 Size: 7831 SbfI CC_TGCA'GG Cuts at: 5183 5183 Size: 7831 ScaI AGT'ACT Cuts at: 6079 7791 6079 Size: 1712 6119 SfcI C'TryA_G Cuts at: 826 1045 2247 2623 3421 3728 4031 4340 4967 Size: 219 1202 376 798 307 303 309 627 Cuts at: 4967 4979 5051 5179 6314 6995 7186 826 Size: 12 72 128 1135 681 191 1471 Fragments arranged by size: 1471 1202 1135 798 681 627 376 309 307 303 219 191 128 72 12 SfoI GGC'GCC Cuts at: 2904 3695 3721 3768 3870 4257 4689 2904 Size: 791 26 47 102 387 432 6046 Fragments arranged by size: 6046 791 432 387 102 47 26 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7831 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7831 SmaI CCC'GGG Cuts at: 1872 3772 4177 5158 1872 Size: 1900 405 981 4545 Fragments arranged by size: 4545 1900 981 405 SmlI C'TyrA_G Cuts at: 820 1017 1943 2336 5935 6836 7098 820 Size: 197 926 393 3599 901 262 1553 Fragments arranged by size: 3599 1553 926 901 393 262 197 SnaBI TAC'GTA Cuts at: 493 5622 493 Size: 5129 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7831 SphI G_CATG'C Cuts at: 1132 5189 1132 Size: 4057 3774 SspI AAT'ATT Cuts at: 5 52 5755 5 Size: 47 5703 2081 Fragments arranged by size: 5703 2081 47 StuI AGG'CCT Cuts at: 1517 2532 1517 Size: 1015 6816 StyI C'CwwG_G Cuts at: 513 1065 2879 2949 3582 4260 5270 513 Size: 552 1814 70 633 678 1010 3074 Fragments arranged by size: 3074 1814 1010 678 633 552 70 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2525 2636 2962 3458 3674 5128 5858 Size: 1255 945 111 326 496 216 1454 730 Cuts at: 5858 5875 6028 6213 6658 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1454 1255 945 730 496 445 326 216 185 153 111 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3018 3054 4326 Size: 274 80 33 51 1443 1041 36 1272 Cuts at: 4326 6077 7789 96 Size: 1751 1712 138 Fragments arranged by size: 1751 1712 1443 1272 1041 274 138 80 51 36 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2809 2841 4772 4804 4934 4966 5070 5102 7791 Size: 32 1931 32 130 32 104 32 2689 Cuts at: 7791 7823 2809 Size: 32 2817 Fragments arranged by size: 2817 2689 1931 130 104 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7831 XbaI T'CTAG_A Cuts at: 5167 5167 Size: 7831 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3424 3646 5122 3424 Size: 222 1476 6133 Fragments arranged by size: 6133 1476 222 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7831 XmaI C'CCGG_G Cuts at: 1870 3770 4175 5156 1870 Size: 1900 405 981 4545 Fragments arranged by size: 4545 1900 981 405 XmnI GAAnn'nnTTC Cuts at: 2696 5960 2696 Size: 3264 4567 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5639 276 Size: 53 83 186 936 4105 2468 Fragments arranged by size: 4105 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV FalI FspAI NsiI PacI PspXI PsrI RsrII SexAI SfiI SrfI SwaI