Restriction map for
pFN21AB4712
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# NO CUT ENZYMES ( 151 enzymes) #
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AatII Acc65I AccI AccIII AcsI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BfaI BfrI
BglII BlnI BsaAI BsaBI BseAI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrFI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EagI Eam1105I
EclXI Eco52I Eco81I EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FseI FspI HincII HindII HindIII
HpaI HphI KasI KpnI MaeI
MamI MfeI MluI MroI MslI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NlaIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI PvuI PvuII
RcaI RsrII SacI SalI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StyI SwaI TfiI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AclI 151 AACGTT AA^CGTT
AcyI 607 GACGCC G[GA]^CG[CT]C
AlwI 555 GATCC GGATC(4/5)
Aor51HI 53 AGCGCT AGC^GCT
AspHI 487 GAGCAC G[AT]GC[AT]^C
BclI 323 TGATCA T^GATCA
BlpI 300 GCTGAGC GC^T[ACGT]AGC
BpmI 177 CTGGAG CTGGAG(16/14)
Bpu1102I 300 GCTGAGC GC^T[ACGT]AGC
BsaHI 607 GACGCC G[GA]^CG[CT]C
BsaI 340 GAGACC GGTCTC(1/5)
BsaJI 71 CCGCGG C^C[ACGT][ACGT]GG
BsaWI 415 ACCGGA [AT]^CCGG[AT]
BseRI 673 CTCCTC GAGGAG(10/8)
BsgI 22 GTGCAG GTGCAG(16/14)
BsiHKAI 487 GAGCAC G[AT]GC[AT]^C
BsmAI 340 GAGAC GTCTC(1/5)
BsmFI 383 GGGAC GGGAC(10/14)
BsrBI 168 CCGCTC CCGCTC(-3/-3)
BsrDI 221 GCAATG GCAATG(2/0)
BssSI 472 CACGAG CACGAG(-5/-1)
BstAPI 15 GCAACTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 554 AGATCC [GA]^GATC[CT]
CelII 300 GCTGAGC GC^T[ACGT]AGC
CfoI 54 GCGC GCG^C
DdeI 301 CTGAG C^T[ACGT]AG
DsaI 71 CCGCGG C^C[GA][CT]GG
EarI 171 CTCTTC CTCTTC(1/4)
Eco47III 53 AGCGCT AGC^GCT
EcoNI 630 CCTCAACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 323 TGATCA T^GATCA
HaeII 53 AGCGCT [GA]GCGC^[CT]
HgaI 607 GACGC GACGC(5/10)
HhaI 54 GCGC GCG^C
Hin1I 607 GACGCC G[GA]^CG[CT]C
HinP1I 54 GCGC G^CGC
Ksp632I 171 CTCTTC CTCTTC(1/4)
KspI 71 CCGCGG CCGC^GG
MaeIII 88 GTGAC ^GT[ACGT]AC
MflI 554 AGATCC [GA]^GATC[CT]
MseI 530 TTAA T^TAA
MspA1I 71 CCGCGG C[AC]G^C[GT]G
NspI 491 ACATGC [GA]CATG^[CT]
Psp1406I 151 AACGTT AA^CGTT
PstI 212 CTGCAG CTGCA^G
SacII 71 CCGCGG CCGC^GG
SapI 170 GCTCTTC GCTCTTC(1/4)
Tru9I 530 TTAA T^TAA
Tsp45I 88 GTGAC ^GT[GC]AC
Tsp509I 331 AATT ^AATT
TspRI 107 CACTG CA[GC]TG[ACGT][ACGT]^
XhoII 554 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 25 enzymes) #
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AfaI 252 GTAC GT^AC
348 GTAC
AvaII 6 GGACC G^G[AT]CC
243 GGTCC
BalI 410 TGGCCA TGG^CCA
587 TGGCCA
BbsI 204 GAAGAC GAAGAC(2/6)
541 GTCTTC
BcnI 32 CCCGG CC^[GC]GG
255 CCGGG
BglI 31 GCCCGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
40 GCCGAGCAGGC
BmyI 161 GGGCAC G[AGT]GC[ACT]^C
487 GAGCAC
BpuAI 204 GAAGAC GAAGAC(2/6)
541 GTCTTC
BsiYI 393 CCGCTACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
630 CCTCAACGAGG
BslI 393 CCGCTACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
630 CCTCAACGAGG
Bsp1286I 161 GGGCAC G[AGT]GC[ACT]^C
487 GAGCAC
BsrI 18 ACTGG ACTGG(1/-1)
345 CCAGT
BstF5I 510 CATCC GGATG(2/0)
294 GGATG
Cfr13I 6 GGACC G^G[ACGT]CC
243 GGTCC
FokI 510 CATCC GGATG(9/13)
294 GGATG
MaeII 86 ACGT A^CGT
152 ACGT
MluNI 410 TGGCCA TGG^CCA
587 TGGCCA
MscI 410 TGGCCA TGG^CCA
587 TGGCCA
NciI 32 CCCGG CC^[GC]GG
255 CCGGG
RsaI 252 GTAC GT^AC
348 GTAC
Sau96I 6 GGACC G^G[ACGT]CC
243 GGTCC
SfcI 212 CTGCAG C^T[GA][CT]AG
459 CTACAG
StuI 455 AGGCCT AGG^CCT
519 AGGCCT
TaqI 112 TCGA T^CGA
602 TCGA
TthHB8I 112 TCGA T^CGA
602 TCGA
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# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AccII AciI AluI BbvI BssKI
BstNI BstUI Cac8I DpnII EaeI
EcoRII Fnu4HI HaeIII HapII HinfI
HpaII ItaI MboI MboII MnlI
MspI MvaI MvnI MwoI NdeII
NlaIII PleI Sau3AI ScrFI TseI