(Circular) (Six-base) MAPSORT of: pFN21AB4698.seq Check: 62 from: 1 to: 7354 pFN21AB4698 7354 bp With 182 enzymes: SgfI * September 11, 2009 17:03 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5164 278 Size: 53 83 186 936 3628 2468 Fragments arranged by size: 3628 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2664 2888 4697 1937 Size: 727 224 1809 4594 Fragments arranged by size: 4594 1809 727 224 Acc65I G'GTAC_C Cuts at: 2700 4675 2700 Size: 1975 5379 AclI AA'CG_TT Cuts at: 1563 4440 5481 5854 1563 Size: 2877 1041 373 3063 Fragments arranged by size: 3063 2877 1041 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2949 5417 6801 7069 1632 Size: 1317 2468 1384 268 1917 Fragments arranged by size: 2468 1917 1384 1317 268 AfeI AGC'GCT Cuts at: 3326 3326 Size: 7354 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7157 AflIII A'CryG_T Cuts at: 1203 2689 6253 7230 1203 Size: 1486 3564 977 1327 Fragments arranged by size: 3564 1486 1327 977 AgeI A'CCGG_T Cuts at: 6185 6185 Size: 7354 AhdI GACnn_n'nnGTC Cuts at: 2287 6083 2287 Size: 3796 3558 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7354 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2547 1184 Size: 1363 5991 AlwNI CAG_nnn'CTG Cuts at: 5097 6669 5097 Size: 1572 5782 ApaI G_GGCC'C Cuts at: 3046 3153 3046 Size: 107 7247 ApaLI G'TGCA_C Cuts at: 5411 6567 5411 Size: 1156 6198 ApoI r'AATT_y Cuts at: 1493 2819 3441 3776 4588 4597 4663 4889 5041 Size: 1326 622 335 812 9 66 226 152 Cuts at: 5041 7150 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1326 812 622 335 226 152 66 9 AseI AT'TA_AT Cuts at: 160 1032 5908 160 Size: 872 4876 1606 Fragments arranged by size: 4876 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7354 AvaI C'yCGr_G Cuts at: 1870 1943 3560 4679 1870 Size: 73 1617 1119 4545 Fragments arranged by size: 4545 1617 1119 73 AvrII C'CTAG_G Cuts at: 2105 2105 Size: 7354 BamHI G'GATC_C Cuts at: 4283 4684 5051 4283 Size: 401 367 6586 Fragments arranged by size: 6586 401 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2111 2700 3812 4675 6130 Size: 325 217 627 324 589 1112 863 1455 Cuts at: 6130 618 Size: 1842 Fragments arranged by size: 1842 1455 1112 863 627 589 325 324 217 BanII G_rGCy'C Cuts at: 1391 2410 2869 3046 3138 3153 3210 4104 4673 Size: 1019 459 177 92 15 57 894 569 Cuts at: 4673 1391 Size: 4072 Fragments arranged by size: 4072 1019 894 569 459 177 92 57 15 BbeI G_GCGC'C Cuts at: 2115 2115 Size: 7354 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6383 BbvCI CC'TCA_GC Cuts at: 3975 3975 Size: 7354 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5545 5579 1903 Size: 34 50 34 3524 34 3678 Fragments arranged by size: 3678 3524 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5246 6456 5246 Size: 1210 6144 BclI T'GATC_A Cuts at: 1548 3576 3897 1548 Size: 2028 321 5005 Fragments arranged by size: 5005 2028 321 BfrBI ATG'CAT Cuts at: 2020 2020 Size: 7354 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5965 136 Size: 107 122 71 631 605 4293 1525 Fragments arranged by size: 4293 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2596 3639 4049 7349 2596 Size: 1043 410 3300 2601 Fragments arranged by size: 3300 2601 1043 410 BlpI GC'TnA_GC Cuts at: 4771 4771 Size: 7354 Bme1580I G_kGCm'C Cuts at: 1790 3015 3046 3153 4426 5415 6571 1790 Size: 1225 31 107 1273 989 1156 2573 Fragments arranged by size: 2573 1273 1225 1156 989 107 31 BmgBI CAC'GTC Cuts at: 1318 2329 1318 Size: 1011 6343 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2236 4295 5085 Size: 416 53 83 399 24 819 2059 790 Cuts at: 5085 6043 442 Size: 958 1753 Fragments arranged by size: 2059 1753 958 819 790 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7354 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2621 2883 3190 4467 6014 1239 Size: 1382 262 307 1277 1547 2579 Fragments arranged by size: 2579 1547 1382 1277 307 262 Bpu10I CC'TnA_GC Cuts at: 2414 3975 2414 Size: 1561 5793 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3257 5479 6344 6642 3257 Size: 2222 865 298 3969 Fragments arranged by size: 3969 2222 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2383 2733 3074 6017 882 Size: 617 884 350 341 2943 2219 Fragments arranged by size: 2943 2219 884 617 350 341 BsaAI yAC'GTr Cuts at: 493 1204 5145 493 Size: 711 3941 2702 Fragments arranged by size: 3941 2702 711 BsaBI GATnn'nnATC Cuts at: 3569 3902 7348 3569 Size: 333 3446 3575 Fragments arranged by size: 3575 3446 333 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2112 4338 5161 5543 Size: 53 83 186 936 579 2226 823 382 Cuts at: 5543 6178 275 Size: 635 1451 Fragments arranged by size: 2226 1451 936 823 635 579 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5787 6185 6459 6606 7017 5787 Size: 398 274 147 411 6124 Fragments arranged by size: 6124 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3453 3483 4010 4040 1178 Size: 30 165 30 2050 30 527 30 4492 Fragments arranged by size: 4492 2050 527 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2268 3743 3898 1186 Size: 1082 1475 155 4642 Fragments arranged by size: 4642 1475 1082 155 BseYI C'CCAG_C Cuts at: 2345 3004 6557 7053 2345 Size: 659 3553 496 2646 Fragments arranged by size: 3553 2646 659 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6781 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4128 4804 5565 6593 1540 Size: 275 186 2127 676 761 1028 2301 Fragments arranged by size: 2301 2127 1028 761 676 275 186 BsiHKAI G_wGCw'C Cuts at: 2410 2869 3138 3210 5415 5500 6571 2410 Size: 459 269 72 2205 85 1071 3193 Fragments arranged by size: 3193 2205 1071 459 269 85 72 BsmI GAATG_Cn' Cuts at: 2986 4561 4865 4958 2986 Size: 1575 304 93 5382 Fragments arranged by size: 5382 1575 304 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2335 2335 Size: 7354 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2410 2869 3015 3046 3138 3153 3210 Size: 399 620 459 146 31 92 15 57 Cuts at: 3210 4104 4426 4673 5415 5500 6571 1391 Size: 894 322 247 742 85 1071 2174 Fragments arranged by size: 2174 1071 894 742 620 459 399 322 247 146 92 85 57 31 15 BspEI T'CCGG_A Cuts at: 7017 7017 Size: 7354 BspHI T'CATG_A Cuts at: 3301 3552 3573 5243 6973 3301 Size: 251 21 1670 1730 3682 Fragments arranged by size: 3682 1730 1670 251 21 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4709 844 Size: 3865 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5241 1394 Size: 429 3418 3507 Fragments arranged by size: 3507 3418 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2712 3325 4491 5849 6023 65 Size: 1183 1464 613 1166 1358 174 1396 Fragments arranged by size: 1464 1396 1358 1183 1166 613 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5998 6185 1700 Size: 110 4188 187 2869 Fragments arranged by size: 4188 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7354 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7354 BssSI C'ACGA_G Cuts at: 5414 6426 5414 Size: 1012 6342 BstAPI GCAn_nnn'nTGC Cuts at: 794 2494 4556 794 Size: 1700 2062 3592 Fragments arranged by size: 3592 2062 1700 BstBI TT'CG_AA Cuts at: 6158 6158 Size: 7354 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7354 BstXI CCAn_nnnn'nTGG Cuts at: 3268 3268 Size: 7354 BstYI r'GATC_y Cuts at: 1972 2596 3639 3848 3979 4049 4283 4684 5051 Size: 624 1043 209 131 70 234 401 367 Cuts at: 5051 5436 5453 6905 6991 7003 7349 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1043 624 401 385 367 346 234 209 131 86 70 17 12 BstZ17I GTA'TAC Cuts at: 2889 2889 Size: 7354 Bsu36I CC'TnA_GG Cuts at: 2945 2945 Size: 7354 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6802 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4252 487 Size: 683 56 3026 3589 Fragments arranged by size: 3589 3026 683 56 BtsI GCAGTG_nn' Cuts at: 2483 3015 4068 4870 5664 5684 2483 Size: 532 1053 802 794 20 4153 Fragments arranged by size: 4153 1053 802 794 532 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7354 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3355 3390 4473 4508 529 Size: 35 2791 35 1083 35 3375 Fragments arranged by size: 3375 2791 1083 35 35 35 DraI TTT'AAA Cuts at: 4658 4658 Size: 7354 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6361 809 Size: 507 5045 1802 Fragments arranged by size: 5045 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4125 4801 5690 Size: 54 1425 50 275 18 2295 676 889 Cuts at: 5690 7319 8 Size: 1629 43 Fragments arranged by size: 2295 1629 1425 889 676 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4125 4801 1537 Size: 275 2313 676 4090 Fragments arranged by size: 4090 2313 676 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2077 3519 3739 5284 1353 Size: 724 1442 220 1545 3423 Fragments arranged by size: 3423 1545 1442 724 220 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5941 6315 6461 387 Size: 1064 138 4352 374 146 1280 Fragments arranged by size: 4352 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2408 2867 3136 3208 2408 Size: 459 269 72 6554 Fragments arranged by size: 6554 459 269 72 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2621 2883 2949 3190 4467 5417 6014 Size: 393 989 262 66 241 1277 950 597 Cuts at: 6014 6801 7069 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1277 989 950 787 597 393 268 262 241 66 EcoNI CCTnn'n_nnAGG Cuts at: 3050 3050 Size: 7354 EcoO109I rG'GnC_Cy Cuts at: 3042 3043 3150 4471 3042 Size: 1 107 1321 5925 Fragments arranged by size: 5925 1321 107 1 EcoRI G'AATT_C Cuts at: 4588 4663 5041 4588 Size: 75 378 6901 Fragments arranged by size: 6901 378 75 EcoRV GAT'ATC Cuts at: 3567 3567 Size: 7354 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4233 4233 Size: 7354 FspI TGC'GCA Cuts at: 5860 5860 Size: 7354 HaeII r_GCGC'y Cuts at: 1678 2115 3328 6501 1678 Size: 437 1213 3173 2531 Fragments arranged by size: 3173 2531 1213 437 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2273 2305 2382 2414 3943 Size: 32 135 32 701 32 77 32 1529 Cuts at: 3943 3975 4010 4042 5995 6027 6069 6101 1373 Size: 32 35 32 1953 32 42 32 2626 Fragments arranged by size: 2626 1953 1529 701 135 77 42 35 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2838 3346 3393 4698 4944 669 Size: 988 281 900 508 47 1305 246 3079 Fragments arranged by size: 3079 1305 988 900 508 281 246 47 HindIII A'AGCT_T Cuts at: 748 748 Size: 7354 HpaI GTT'AAC Cuts at: 4944 4944 Size: 7354 KasI G'GCGC_C Cuts at: 2111 2111 Size: 7354 KpnI G_GTAC'C Cuts at: 2704 4679 2704 Size: 1975 5379 MfeI C'AATT_G Cuts at: 3396 3492 4953 3396 Size: 96 1461 5797 Fragments arranged by size: 5797 1461 96 MluI A'CGCG_T Cuts at: 7230 7230 Size: 7354 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3439 3850 4158 6468 6652 1305 Size: 2134 411 308 2310 184 2007 Fragments arranged by size: 2310 2134 2007 411 308 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5532 Fragments arranged by size: 5532 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2384 5312 5671 5830 518 Size: 648 1218 2928 359 159 2042 Fragments arranged by size: 2928 2042 1218 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4770 5447 6595 6840 1606 Size: 3164 677 1148 245 2120 Fragments arranged by size: 3164 2120 1148 677 245 NarI GG'CG_CC Cuts at: 2112 2112 Size: 7354 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6802 NdeI CA'TA_TG Cuts at: 387 387 Size: 7354 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7354 NotI GC'GGCC_GC Cuts at: 4801 4801 Size: 7354 NsiI A_TGCA'T Cuts at: 2022 2022 Size: 7354 NspI r_CATG'y Cuts at: 1132 1229 4090 4712 6257 1132 Size: 97 2861 622 1545 2229 Fragments arranged by size: 2861 2229 1545 622 97 PasI CC'CwG_GG Cuts at: 1348 3015 1348 Size: 1667 5687 PciI A'CATG_T Cuts at: 6253 6253 Size: 7354 PflMI CCAn_nnn'nTGG Cuts at: 1108 2360 1108 Size: 1252 6102 PmeI GTTT'AAAC Cuts at: 4658 4658 Size: 7354 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5423 5455 6936 6968 5423 Size: 32 1481 32 5809 Fragments arranged by size: 5809 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7354 PsiI TTA'TAA Cuts at: 4611 4924 4611 Size: 313 7041 PspOMI G'GGCC_C Cuts at: 3042 3149 3042 Size: 107 7247 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2133 2165 2133 Size: 32 7322 PstI C_TGCA'G Cuts at: 830 3358 4706 830 Size: 2528 1348 3478 Fragments arranged by size: 3478 2528 1348 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7354 SacI G_AGCT'C Cuts at: 2410 2869 3138 3210 2410 Size: 459 269 72 6554 Fragments arranged by size: 6554 459 269 72 SalI G'TCGA_C Cuts at: 1936 4696 1936 Size: 2760 4594 SbfI CC_TGCA'GG Cuts at: 4706 4706 Size: 7354 ScaI AGT'ACT Cuts at: 5602 7314 5602 Size: 1712 5642 SexAI A'CCwGG_T Cuts at: 2667 2667 Size: 7354 SfcI C'TryA_G Cuts at: 826 1045 3354 3942 4702 5837 6518 6709 826 Size: 219 2309 588 760 1135 681 191 1471 Fragments arranged by size: 2309 1471 1135 760 681 588 219 191 SfoI GGC'GCC Cuts at: 2113 2113 Size: 7354 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7354 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7354 SmaI CCC'GGG Cuts at: 1872 4681 1872 Size: 2809 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3236 5458 6359 6621 820 Size: 197 926 1293 2222 901 262 1553 Fragments arranged by size: 2222 1553 1293 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5145 493 Size: 4652 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7354 SphI G_CATG'C Cuts at: 1132 4712 1132 Size: 3580 3774 SspI AAT'ATT Cuts at: 5 52 4567 5278 5 Size: 47 4515 711 2081 Fragments arranged by size: 4515 2081 711 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7354 StyI C'CwwG_G Cuts at: 513 1065 2105 3807 4392 4474 4793 513 Size: 552 1040 1702 585 82 319 3074 Fragments arranged by size: 3074 1702 1040 585 552 319 82 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3800 5381 5398 5551 5736 6181 325 Size: 1255 2220 1581 17 153 185 445 1498 Fragments arranged by size: 2220 1581 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3384 4197 5600 Size: 274 80 33 51 1443 1407 813 1403 Cuts at: 5600 7312 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1407 1403 813 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7314 7346 7314 Size: 32 7322 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7354 XbaI T'CTAG_A Cuts at: 4690 4690 Size: 7354 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3012 3012 Size: 7354 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7354 XmaI C'CCGG_G Cuts at: 1870 4679 1870 Size: 2809 4545 XmnI GAAnn'nnTTC Cuts at: 5483 5483 Size: 7354 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5162 276 Size: 53 83 186 936 3628 2468 Fragments arranged by size: 3628 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI BaeI BplI BsiWI DraIII FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI PvuII RsrII SacII SanDI SapI SfiI SrfI SwaI