(Circular) (Six-base) MAPSORT of: pFN21AB4689.seq Check: 3079 from: 1 to: 6025 pFN21AB4689 6025 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3835 278 Size: 53 83 186 936 2299 2468 Fragments arranged by size: 2468 2299 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3368 1937 Size: 1431 4594 Acc65I G'GTAC_C Cuts at: 3346 3346 Size: 6025 AclI AA'CG_TT Cuts at: 1563 4152 4525 1563 Size: 2589 373 3063 Fragments arranged by size: 3063 2589 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2135 2327 4088 5472 5740 1632 Size: 503 192 1761 1384 268 1917 Fragments arranged by size: 1917 1761 1384 503 268 192 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5828 AflIII A'CryG_T Cuts at: 1203 4924 5901 1203 Size: 3721 977 1327 Fragments arranged by size: 3721 1327 977 AgeI A'CCGG_T Cuts at: 4856 4856 Size: 6025 AhdI GACnn_n'nnGTC Cuts at: 4754 4754 Size: 6025 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6025 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6025 AlwNI CAG_nnn'CTG Cuts at: 3768 5340 3768 Size: 1572 4453 ApaLI G'TGCA_C Cuts at: 2595 4082 5238 2595 Size: 1487 1156 3382 Fragments arranged by size: 3382 1487 1156 ApoI r'AATT_y Cuts at: 1493 2057 2301 3334 3560 3712 5821 1493 Size: 564 244 1033 226 152 2109 1697 Fragments arranged by size: 2109 1697 1033 564 244 226 152 AseI AT'TA_AT Cuts at: 160 1032 4579 160 Size: 872 3547 1606 Fragments arranged by size: 3547 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6025 AvaI C'yCGr_G Cuts at: 1870 1943 2255 3350 1870 Size: 73 312 1095 4545 Fragments arranged by size: 4545 1095 312 73 BamHI G'GATC_C Cuts at: 3218 3355 3722 3218 Size: 137 367 5521 Fragments arranged by size: 5521 367 137 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3346 4801 618 Size: 325 217 627 1559 1455 1842 Fragments arranged by size: 1842 1559 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2786 2845 2993 3344 1391 Size: 1395 59 148 351 4072 Fragments arranged by size: 4072 1395 351 148 59 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5054 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4216 4250 1903 Size: 34 2279 34 3678 Fragments arranged by size: 3678 2279 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3917 5127 3917 Size: 1210 4815 BclI T'GATC_A Cuts at: 1548 1548 Size: 6025 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4636 136 Size: 107 122 71 631 605 2964 1525 Fragments arranged by size: 2964 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2108 6020 2108 Size: 3912 2113 BlpI GC'TnA_GC Cuts at: 3442 3442 Size: 6025 Bme1580I G_kGCm'C Cuts at: 1790 2599 4086 5242 1790 Size: 809 1487 1156 2573 Fragments arranged by size: 2573 1487 1156 809 BmgBI CAC'GTC Cuts at: 1318 2594 1318 Size: 1276 4749 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2675 3756 4714 Size: 416 53 83 399 24 1258 1081 958 Cuts at: 4714 442 Size: 1753 Fragments arranged by size: 1753 1258 1081 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6025 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2940 4685 1239 Size: 1701 1745 2579 Fragments arranged by size: 2579 1745 1701 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4150 5015 5313 4150 Size: 865 298 4862 Fragments arranged by size: 4862 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2880 2908 2916 4688 882 Size: 617 1381 28 8 1772 2219 Fragments arranged by size: 2219 1772 1381 617 28 8 BsaAI yAC'GTr Cuts at: 493 1204 3816 493 Size: 711 2612 2702 Fragments arranged by size: 2702 2612 711 BsaBI GATnn'nnATC Cuts at: 6019 6019 Size: 6025 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2677 2743 3832 4214 Size: 53 83 186 936 1144 66 1089 382 Cuts at: 4214 4849 275 Size: 635 1451 Fragments arranged by size: 1451 1144 1089 936 635 382 186 83 66 53 BsaWI w'CCGG_w Cuts at: 3032 4458 4856 5130 5277 5688 3032 Size: 1426 398 274 147 411 3369 Fragments arranged by size: 3369 1426 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5800 Fragments arranged by size: 5800 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2799 1186 Size: 1613 4412 BseYI C'CCAG_C Cuts at: 2165 5228 5724 2165 Size: 3063 496 2466 Fragments arranged by size: 3063 2466 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5452 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2827 3475 4236 5264 1540 Size: 275 186 826 648 761 1028 2301 Fragments arranged by size: 2301 1028 826 761 648 275 186 BsiHKAI G_wGCw'C Cuts at: 2599 2845 4086 4171 5242 2599 Size: 246 1241 85 1071 3382 Fragments arranged by size: 3382 1241 1071 246 85 BsmI GAATG_Cn' Cuts at: 2142 2167 2486 2727 3274 3536 3629 2142 Size: 25 319 241 547 262 93 4538 Fragments arranged by size: 4538 547 319 262 241 93 25 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2599 2786 2845 2993 3344 4086 4171 Size: 399 809 187 59 148 351 742 85 Cuts at: 4171 5242 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 809 742 399 351 187 148 85 59 BspEI T'CCGG_A Cuts at: 3032 5688 3032 Size: 2656 3369 BspHI T'CATG_A Cuts at: 3175 3914 5644 3175 Size: 739 1730 3556 Fragments arranged by size: 3556 1730 739 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3380 844 Size: 2536 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3912 1394 Size: 429 2089 3507 Fragments arranged by size: 3507 2089 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4520 4694 65 Size: 1183 3272 174 1396 Fragments arranged by size: 3272 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4669 4856 1700 Size: 110 2859 187 2869 Fragments arranged by size: 2869 2859 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6025 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6025 BssSI C'ACGA_G Cuts at: 4085 5097 4085 Size: 1012 5013 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6025 BstBI TT'CG_AA Cuts at: 4829 4829 Size: 6025 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6025 BstXI CCAn_nnnn'nTGG Cuts at: 3000 3124 3000 Size: 124 5901 BstYI r'GATC_y Cuts at: 1972 2108 3131 3218 3355 3722 4107 4124 5576 Size: 136 1023 87 137 367 385 17 1452 Cuts at: 5576 5662 5674 6020 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1023 385 367 346 137 136 87 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4534 Fragments arranged by size: 4534 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5286 Fragments arranged by size: 5286 683 56 BtsI GCAGTG_nn' Cuts at: 2178 2254 3541 4335 4355 2178 Size: 76 1287 794 20 3848 Fragments arranged by size: 3848 1287 794 76 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6025 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5990 DraI TTT'AAA Cuts at: 2104 3329 2104 Size: 1225 4800 DraIII CAC_nnn'GTG Cuts at: 3026 3026 Size: 6025 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5032 809 Size: 507 3716 1802 Fragments arranged by size: 3716 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2824 3472 4361 Size: 54 1425 50 275 18 994 648 889 Cuts at: 4361 5990 8 Size: 1629 43 Fragments arranged by size: 1629 1425 994 889 648 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2824 3472 1537 Size: 275 1012 648 4090 Fragments arranged by size: 4090 1012 648 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2082 2325 2698 2758 2862 2902 3955 1353 Size: 729 243 373 60 104 40 1053 3423 Fragments arranged by size: 3423 1053 729 373 243 104 60 40 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4612 4986 5132 387 Size: 1064 138 3023 374 146 1280 Fragments arranged by size: 3023 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2843 2843 Size: 6025 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2135 2327 2940 4088 4685 5472 5740 Size: 393 503 192 613 1148 597 787 268 Cuts at: 5740 1239 Size: 1524 Fragments arranged by size: 1524 1148 787 613 597 503 393 268 192 EcoNI CCTnn'n_nnAGG Cuts at: 2790 3213 2790 Size: 423 5602 EcoRI G'AATT_C Cuts at: 3334 3712 3334 Size: 378 5647 FspI TGC'GCA Cuts at: 4531 4531 Size: 6025 HaeII r_GCGC'y Cuts at: 1678 5172 1678 Size: 3494 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2555 2587 2773 2805 3177 Size: 32 135 32 983 32 186 32 372 Cuts at: 3177 3209 4666 4698 4740 4772 1373 Size: 32 1457 32 42 32 2626 Fragments arranged by size: 2626 1457 983 372 186 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3369 3615 669 Size: 988 281 1431 246 3079 Fragments arranged by size: 3079 1431 988 281 246 HindIII A'AGCT_T Cuts at: 748 2468 748 Size: 1720 4305 HpaI GTT'AAC Cuts at: 3615 3615 Size: 6025 KpnI G_GTAC'C Cuts at: 3350 3350 Size: 6025 MfeI C'AATT_G Cuts at: 2292 3624 2292 Size: 1332 4693 MluI A'CGCG_T Cuts at: 5901 5901 Size: 6025 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5139 5323 1305 Size: 3834 184 2007 Fragments arranged by size: 3834 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4203 Fragments arranged by size: 4203 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2998 3983 4342 4501 518 Size: 648 1832 985 359 159 2042 Fragments arranged by size: 2042 1832 985 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3157 3441 4118 5266 5511 1606 Size: 1551 284 677 1148 245 2120 Fragments arranged by size: 2120 1551 1148 677 284 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4534 Fragments arranged by size: 4534 939 552 NdeI CA'TA_TG Cuts at: 387 3070 387 Size: 2683 3342 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6025 NotI GC'GGCC_GC Cuts at: 3472 3472 Size: 6025 NspI r_CATG'y Cuts at: 1132 1229 3383 4928 1132 Size: 97 2154 1545 2229 Fragments arranged by size: 2229 2154 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6025 PciI A'CATG_T Cuts at: 4924 4924 Size: 6025 PflMI CCAn_nnn'nTGG Cuts at: 1108 3026 1108 Size: 1918 4107 PfoI T'CCnGG_A Cuts at: 2475 2812 2475 Size: 337 5688 PmeI GTTT'AAAC Cuts at: 3329 3329 Size: 6025 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4094 4126 5607 5639 4094 Size: 32 1481 32 4480 Fragments arranged by size: 4480 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6025 PsiI TTA'TAA Cuts at: 3595 3595 Size: 6025 PstI C_TGCA'G Cuts at: 830 2077 3288 3377 830 Size: 1247 1211 89 3478 Fragments arranged by size: 3478 1247 1211 89 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6025 SacI G_AGCT'C Cuts at: 2845 2845 Size: 6025 SalI G'TCGA_C Cuts at: 1936 3367 1936 Size: 1431 4594 SbfI CC_TGCA'GG Cuts at: 3377 3377 Size: 6025 ScaI AGT'ACT Cuts at: 4273 5985 4273 Size: 1712 4313 SfcI C'TryA_G Cuts at: 826 1045 2073 3284 3373 4508 5189 5380 826 Size: 219 1028 1211 89 1135 681 191 1471 Fragments arranged by size: 1471 1211 1135 1028 681 219 191 89 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6025 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6025 SmaI CCC'GGG Cuts at: 1872 3352 1872 Size: 1480 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4129 5030 5292 820 Size: 197 926 2186 901 262 1553 Fragments arranged by size: 2186 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3816 493 Size: 3323 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6025 SphI G_CATG'C Cuts at: 1132 3383 1132 Size: 2251 3774 SspI AAT'ATT Cuts at: 5 52 2197 3949 5 Size: 47 2145 1752 2081 Fragments arranged by size: 2145 2081 1752 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6025 StyI C'CwwG_G Cuts at: 513 1065 2004 2325 3464 513 Size: 552 939 321 1139 3074 Fragments arranged by size: 3074 1139 939 552 321 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4052 4069 4222 4407 4852 325 Size: 1255 2472 17 153 185 445 1498 Fragments arranged by size: 2472 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4271 5983 96 Size: 274 80 33 51 1443 2294 1712 138 Fragments arranged by size: 2294 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2667 2699 5985 6017 2667 Size: 32 3286 32 2675 Fragments arranged by size: 3286 2675 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6025 XbaI T'CTAG_A Cuts at: 2111 2759 3361 2111 Size: 648 602 4775 Fragments arranged by size: 4775 648 602 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6025 XmaI C'CCGG_G Cuts at: 1870 3350 1870 Size: 1480 4545 XmnI GAAnn'nnTTC Cuts at: 4154 4154 Size: 6025 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3833 276 Size: 53 83 186 936 2299 2468 Fragments arranged by size: 2468 2299 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI