(Circular) (Six-base) MAPSORT of: pFN21AB4670.seq Check: 8780 from: 1 to: 5908 pFN21AB4670 5908 bp With 182 enzymes: SgfI * October 20, 2009 10:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2343 3718 278 Size: 53 83 186 936 807 1375 2468 Fragments arranged by size: 2468 1375 936 807 186 83 53 AccI GT'mk_AC Cuts at: 1937 3251 1937 Size: 1314 4594 Acc65I G'GTAC_C Cuts at: 3229 3229 Size: 5908 AclI AA'CG_TT Cuts at: 1563 4035 4408 1563 Size: 2472 373 3063 Fragments arranged by size: 3063 2472 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3042 3971 5355 5623 1632 Size: 1410 929 1384 268 1917 Fragments arranged by size: 1917 1410 1384 929 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5711 AflIII A'CryG_T Cuts at: 1203 4807 5784 1203 Size: 3604 977 1327 Fragments arranged by size: 3604 1327 977 AgeI A'CCGG_T Cuts at: 4739 4739 Size: 5908 AhdI GACnn_n'nnGTC Cuts at: 4637 4637 Size: 5908 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5908 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5908 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2043 2075 2043 Size: 32 5876 AlwNI CAG_nnn'CTG Cuts at: 3651 5223 3651 Size: 1572 4336 ApaI G_GGCC'C Cuts at: 2709 2709 Size: 5908 ApaLI G'TGCA_C Cuts at: 3965 5121 3965 Size: 1156 4752 ApoI r'AATT_y Cuts at: 1493 3217 3443 3595 5704 1493 Size: 1724 226 152 2109 1697 Fragments arranged by size: 2109 1724 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 2988 4462 160 Size: 872 1956 1474 1606 Fragments arranged by size: 1956 1606 1474 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5908 AvaI C'yCGr_G Cuts at: 1870 1943 2313 3233 1870 Size: 73 370 920 4545 Fragments arranged by size: 4545 920 370 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2894 2927 2894 Size: 33 5875 BamHI G'GATC_C Cuts at: 2201 3138 3238 3605 2201 Size: 937 100 367 4504 Fragments arranged by size: 4504 937 367 100 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2250 2696 3229 4684 618 Size: 325 217 627 463 446 533 1455 1842 Fragments arranged by size: 1842 1455 627 533 463 446 325 217 BanII G_rGCy'C Cuts at: 1391 2709 3227 1391 Size: 1318 518 4072 Fragments arranged by size: 4072 1318 518 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2371 928 Size: 971 472 4465 Fragments arranged by size: 4465 971 472 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4099 4133 1903 Size: 34 2162 34 3678 Fragments arranged by size: 3678 2162 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3800 5010 3800 Size: 1210 4698 BclI T'GATC_A Cuts at: 1548 1548 Size: 5908 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4519 136 Size: 107 122 71 631 605 2847 1525 Fragments arranged by size: 2847 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5903 5903 Size: 5908 BlpI GC'TnA_GC Cuts at: 3325 3325 Size: 5908 Bme1580I G_kGCm'C Cuts at: 1790 2253 2709 3969 5125 1790 Size: 463 456 1260 1156 2573 Fragments arranged by size: 2573 1260 1156 463 456 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5908 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3639 4597 442 Size: 416 53 83 399 24 2222 958 1753 Fragments arranged by size: 2222 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5908 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2903 2903 Size: 5908 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2343 2695 4568 1239 Size: 1104 352 1873 2579 Fragments arranged by size: 2579 1873 1104 352 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4033 4898 5196 4033 Size: 865 298 4745 Fragments arranged by size: 4745 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2073 2655 4571 882 Size: 617 574 582 1916 2219 Fragments arranged by size: 2219 1916 617 582 574 BsaAI yAC'GTr Cuts at: 493 1204 3699 493 Size: 711 2495 2702 Fragments arranged by size: 2702 2495 711 BsaBI GATnn'nnATC Cuts at: 2808 5902 2808 Size: 3094 2814 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2217 2340 3715 4097 Size: 53 83 186 936 684 123 1375 382 Cuts at: 4097 4732 275 Size: 635 1451 Fragments arranged by size: 1451 1375 936 684 635 382 186 123 83 53 BsaWI w'CCGG_w Cuts at: 2812 4341 4739 5013 5160 5571 2812 Size: 1529 398 274 147 411 3149 Fragments arranged by size: 3149 1529 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2043 2073 2293 2323 2330 Size: 30 165 30 640 30 220 30 7 Cuts at: 2330 2360 2573 2603 1178 Size: 30 213 30 4483 Fragments arranged by size: 4483 640 220 213 165 30 30 30 30 30 30 7 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2338 2611 1186 Size: 1152 273 4483 Fragments arranged by size: 4483 1152 273 BseYI C'CCAG_C Cuts at: 5111 5607 5111 Size: 496 5412 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2290 1163 Size: 573 554 4781 Fragments arranged by size: 4781 573 554 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2099 3358 4119 5147 1540 Size: 275 186 98 1259 761 1028 2301 Fragments arranged by size: 2301 1259 1028 761 275 186 98 BsiHKAI G_wGCw'C Cuts at: 3969 4054 5125 3969 Size: 85 1071 4752 Fragments arranged by size: 4752 1071 85 BsmI GAATG_Cn' Cuts at: 3419 3512 3419 Size: 93 5815 BsmBI CGTCTCn'nnnn_ Cuts at: 2331 2574 2331 Size: 243 5665 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2253 2709 3227 3969 4054 5125 1391 Size: 399 463 456 518 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 518 463 456 399 85 BspEI T'CCGG_A Cuts at: 2812 5571 2812 Size: 2759 3149 BspHI T'CATG_A Cuts at: 2602 2874 3797 5527 2602 Size: 272 923 1730 2983 Fragments arranged by size: 2983 1730 923 272 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2312 2380 3029 3263 844 Size: 1468 68 649 234 3489 Fragments arranged by size: 3489 1468 649 234 68 BsrBI CCG'CTC Cuts at: 1394 1823 2137 2736 3795 1394 Size: 429 314 599 1059 3507 Fragments arranged by size: 3507 1059 599 429 314 BsrDI GCAATG_nn' Cuts at: 65 1248 3200 4403 4577 65 Size: 1183 1952 1203 174 1396 Fragments arranged by size: 1952 1396 1203 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2188 4552 4739 1700 Size: 110 378 2364 187 2869 Fragments arranged by size: 2869 2364 378 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5908 BssHII G'CGCG_C Cuts at: 1925 2160 1925 Size: 235 5673 BssSI C'ACGA_G Cuts at: 3968 4980 3968 Size: 1012 4896 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5908 BstBI TT'CG_AA Cuts at: 4712 4712 Size: 5908 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5908 BstYI r'GATC_y Cuts at: 1972 2201 3138 3238 3605 3990 4007 5459 5545 Size: 229 937 100 367 385 17 1452 86 Cuts at: 5545 5557 5903 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 937 385 367 346 229 100 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2093 2125 3099 513 Size: 552 939 89 32 974 3322 Fragments arranged by size: 3322 974 939 552 89 32 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2356 487 Size: 683 56 1130 4039 Fragments arranged by size: 4039 1130 683 56 BtsI GCAGTG_nn' Cuts at: 2755 3424 4218 4238 2755 Size: 669 794 20 4425 Fragments arranged by size: 4425 794 669 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5908 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5873 DraI TTT'AAA Cuts at: 3212 3212 Size: 5908 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2375 4915 809 Size: 507 1059 2540 1802 Fragments arranged by size: 2540 1802 1059 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2096 2190 3355 Size: 54 1425 50 275 18 266 94 1165 Cuts at: 3355 4244 5873 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1165 889 275 266 94 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2096 3355 1537 Size: 275 284 1259 4090 Fragments arranged by size: 4090 1259 284 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2894 2916 3838 1353 Size: 1541 22 922 3423 Fragments arranged by size: 3423 1541 922 22 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2349 4495 4869 5015 387 Size: 1064 138 760 2146 374 146 1280 Fragments arranged by size: 2146 1280 1064 760 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2343 2695 3042 3971 4568 5355 5623 Size: 393 711 352 347 929 597 787 268 Cuts at: 5623 1239 Size: 1524 Fragments arranged by size: 1524 929 787 711 597 393 352 347 268 EcoNI CCTnn'n_nnAGG Cuts at: 2793 2793 Size: 5908 EcoO109I rG'GnC_Cy Cuts at: 2705 2706 3152 2705 Size: 1 446 5461 Fragments arranged by size: 5461 446 1 EcoRI G'AATT_C Cuts at: 3217 3595 3217 Size: 378 5530 FspI TGC'GCA Cuts at: 4414 4414 Size: 5908 HaeII r_GCGC'y Cuts at: 1678 5055 1678 Size: 3377 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2193 2225 2293 2325 2330 Size: 32 135 32 621 32 68 32 5 Cuts at: 2330 2362 2573 2605 2663 2695 4549 4581 4623 Size: 32 211 32 58 32 1854 32 42 Cuts at: 4623 4655 1373 Size: 32 2626 Fragments arranged by size: 2626 1854 621 211 135 68 58 42 32 32 32 32 32 32 32 32 32 5 HincII GTy'rAC Cuts at: 669 1657 1938 2212 3252 3498 669 Size: 988 281 274 1040 246 3079 Fragments arranged by size: 3079 1040 988 281 274 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5908 HpaI GTT'AAC Cuts at: 3498 3498 Size: 5908 KpnI G_GTAC'C Cuts at: 3233 3233 Size: 5908 MfeI C'AATT_G Cuts at: 3507 3507 Size: 5908 MluI A'CGCG_T Cuts at: 5784 5784 Size: 5908 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2376 3096 5022 5206 1305 Size: 1071 720 1926 184 2007 Fragments arranged by size: 2007 1926 1071 720 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4086 Fragments arranged by size: 4086 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2178 2670 3176 3866 4225 4384 518 Size: 648 1012 492 506 690 359 159 2042 Fragments arranged by size: 2042 1012 690 648 506 492 359 159 MspA1I CmG'CkG Cuts at: 1606 2095 2207 3324 4001 5149 5394 1606 Size: 489 112 1117 677 1148 245 2120 Fragments arranged by size: 2120 1148 1117 677 489 245 112 NaeI GCC'GGC Cuts at: 2190 2190 Size: 5908 NcoI C'CATG_G Cuts at: 513 1065 2004 3099 513 Size: 552 939 1095 3322 Fragments arranged by size: 3322 1095 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5908 NgoMIV G'CCGG_C Cuts at: 2188 2188 Size: 5908 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5908 NotI GC'GGCC_GC Cuts at: 3355 3355 Size: 5908 NspI r_CATG'y Cuts at: 1132 1229 3266 4811 1132 Size: 97 2037 1545 2229 Fragments arranged by size: 2229 2037 1545 97 PasI CC'CwG_GG Cuts at: 1348 2702 1348 Size: 1354 4554 PciI A'CATG_T Cuts at: 4807 4807 Size: 5908 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5908 PmeI GTTT'AAAC Cuts at: 3212 3212 Size: 5908 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2043 2075 3977 4009 5490 5522 2043 Size: 32 1902 32 1481 32 2429 Fragments arranged by size: 2429 1902 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3152 3152 Size: 5908 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5908 PsiI TTA'TAA Cuts at: 3478 3478 Size: 5908 PspOMI G'GGCC_C Cuts at: 2705 2705 Size: 5908 PstI C_TGCA'G Cuts at: 830 3151 3260 830 Size: 2321 109 3478 Fragments arranged by size: 3478 2321 109 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5908 SacII CC_GC'GG Cuts at: 2096 2096 Size: 5908 SalI G'TCGA_C Cuts at: 1936 3250 1936 Size: 1314 4594 SapI GCTCTTCn'nnn_ Cuts at: 2894 2916 2894 Size: 22 5886 SbfI CC_TGCA'GG Cuts at: 3151 3260 3151 Size: 109 5799 ScaI AGT'ACT Cuts at: 2463 4156 5868 2463 Size: 1693 1712 2503 Fragments arranged by size: 2503 1712 1693 SexAI A'CCwGG_T Cuts at: 2788 2788 Size: 5908 SfcI C'TryA_G Cuts at: 826 1045 3147 3256 4391 5072 5263 826 Size: 219 2102 109 1135 681 191 1471 Fragments arranged by size: 2102 1471 1135 681 219 191 109 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5908 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5908 SmaI CCC'GGG Cuts at: 1872 2315 3235 1872 Size: 443 920 4545 Fragments arranged by size: 4545 920 443 SmlI C'TyrA_G Cuts at: 820 1017 1943 4012 4913 5175 820 Size: 197 926 2069 901 262 1553 Fragments arranged by size: 2069 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3699 493 Size: 3206 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5908 SphI G_CATG'C Cuts at: 1132 3266 1132 Size: 2134 3774 SspI AAT'ATT Cuts at: 5 52 3832 5 Size: 47 3780 2081 Fragments arranged by size: 3780 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5908 StyI C'CwwG_G Cuts at: 513 1065 2004 3067 3099 3347 513 Size: 552 939 1063 32 248 3074 Fragments arranged by size: 3074 1063 939 552 248 32 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3935 3952 4105 4290 4735 325 Size: 1255 2355 17 153 185 445 1498 Fragments arranged by size: 2355 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2461 3007 4154 Size: 274 80 33 51 1443 484 546 1147 Cuts at: 4154 5866 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1147 546 484 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5868 5900 5868 Size: 32 5876 Tth111I GACn'n_nGTC Cuts at: 1316 2564 1316 Size: 1248 4660 XbaI T'CTAG_A Cuts at: 3244 3244 Size: 5908 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5908 XmaI C'CCGG_G Cuts at: 1870 2313 3233 1870 Size: 443 920 4545 Fragments arranged by size: 4545 920 443 XmnI GAAnn'nnTTC Cuts at: 4037 4037 Size: 5908 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2341 3716 276 Size: 53 83 186 936 807 1375 2468 Fragments arranged by size: 2468 1375 936 807 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SanDI SfiI SfoI SrfI SwaI XcmI