(Circular) (Six-base) MAPSORT of: pFN21AB4640.seq Check: 1786 from: 1 to: 6547 pFN21AB4640 6547 bp With 182 enzymes: SgfI * October 20, 2009 10:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4357 278 Size: 53 83 186 936 2821 2468 Fragments arranged by size: 2821 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3890 1937 Size: 1953 4594 Acc65I G'GTAC_C Cuts at: 3868 3868 Size: 6547 AclI AA'CG_TT Cuts at: 1563 4674 5047 1563 Size: 3111 373 3063 Fragments arranged by size: 3111 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2421 3122 3320 3567 4610 5994 6262 1632 Size: 789 701 198 247 1043 1384 268 1917 Fragments arranged by size: 1917 1384 1043 789 701 268 247 198 AflII C'TTAA_G Cuts at: 820 1017 2393 820 Size: 197 1376 4974 Fragments arranged by size: 4974 1376 197 AflIII A'CryG_T Cuts at: 1203 5446 6423 1203 Size: 4243 977 1327 Fragments arranged by size: 4243 1327 977 AgeI A'CCGG_T Cuts at: 5378 5378 Size: 6547 AhdI GACnn_n'nnGTC Cuts at: 5276 5276 Size: 6547 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6547 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6547 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3726 3758 3726 Size: 32 6515 AlwNI CAG_nnn'CTG Cuts at: 2733 4290 5862 2733 Size: 1557 1572 3418 Fragments arranged by size: 3418 1572 1557 ApaI G_GGCC'C Cuts at: 2535 2535 Size: 6547 ApaLI G'TGCA_C Cuts at: 4604 5760 4604 Size: 1156 5391 ApoI r'AATT_y Cuts at: 1493 2487 3856 4082 4234 6343 1493 Size: 994 1369 226 152 2109 1697 Fragments arranged by size: 2109 1697 1369 994 226 152 AseI AT'TA_AT Cuts at: 160 1032 5101 160 Size: 872 4069 1606 Fragments arranged by size: 4069 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6547 AvaI C'yCGr_G Cuts at: 1870 1943 3872 1870 Size: 73 1929 4545 Fragments arranged by size: 4545 1929 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3028 3061 3028 Size: 33 6514 BamHI G'GATC_C Cuts at: 3877 4244 3877 Size: 367 6180 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3868 5323 618 Size: 325 217 627 2081 1455 1842 Fragments arranged by size: 2081 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2535 3231 3866 1391 Size: 1144 696 635 4072 Fragments arranged by size: 4072 1144 696 635 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3555 3678 928 Size: 971 1656 123 3797 Fragments arranged by size: 3797 1656 971 123 BbvCI CC'TCA_GC Cuts at: 3650 3650 Size: 6547 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4738 4772 1903 Size: 34 2801 34 3678 Fragments arranged by size: 3678 2801 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4439 5649 4439 Size: 1210 5337 BclI T'GATC_A Cuts at: 1548 2887 1548 Size: 1339 5208 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5158 136 Size: 107 122 71 631 605 3486 1525 Fragments arranged by size: 3486 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2341 6542 2341 Size: 4201 2346 BlpI GC'TnA_GC Cuts at: 2271 3964 2271 Size: 1693 4854 Bme1580I G_kGCm'C Cuts at: 1790 2535 4608 5764 1790 Size: 745 2073 1156 2573 Fragments arranged by size: 2573 2073 1156 745 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6547 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2387 3628 4278 Size: 416 53 83 399 24 970 1241 650 Cuts at: 4278 5236 442 Size: 958 1753 Fragments arranged by size: 1753 1241 970 958 650 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6547 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2020 2342 2390 2561 3687 5207 1239 Size: 781 322 48 171 1126 1520 2579 Fragments arranged by size: 2579 1520 1126 781 322 171 48 Bpu10I CC'TnA_GC Cuts at: 2493 3348 3650 2493 Size: 855 302 5390 Fragments arranged by size: 5390 855 302 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2774 4672 5537 5835 2774 Size: 1898 865 298 3486 Fragments arranged by size: 3486 1898 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2475 5210 882 Size: 617 976 2735 2219 Fragments arranged by size: 2735 2219 976 617 BsaAI yAC'GTr Cuts at: 493 1204 4338 493 Size: 711 3134 2702 Fragments arranged by size: 3134 2702 711 BsaBI GATnn'nnATC Cuts at: 6541 6541 Size: 6547 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4354 4736 5371 275 Size: 53 83 186 936 2821 382 635 1451 Fragments arranged by size: 2821 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2585 4980 5378 5652 5799 6210 2585 Size: 2395 398 274 147 411 2922 Fragments arranged by size: 2922 2395 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3269 3299 1178 Size: 30 165 30 1866 30 4426 Fragments arranged by size: 4426 1866 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2264 2777 3221 3825 1186 Size: 1078 513 444 604 3908 Fragments arranged by size: 3908 1078 604 513 444 BseYI C'CCAG_C Cuts at: 2663 3149 5750 6246 2663 Size: 486 2601 496 2964 Fragments arranged by size: 2964 2601 496 486 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3217 3235 1163 Size: 573 1481 18 4475 Fragments arranged by size: 4475 1481 573 18 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3997 4758 5786 1540 Size: 275 186 1996 761 1028 2301 Fragments arranged by size: 2301 1996 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2499 4608 4693 5764 2499 Size: 2109 85 1071 3282 Fragments arranged by size: 3282 2109 1071 85 BsmI GAATG_Cn' Cuts at: 4058 4151 4058 Size: 93 6454 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2499 2535 3231 3866 4608 4693 5764 Size: 399 709 36 696 635 742 85 1071 Cuts at: 5764 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 709 696 635 399 85 36 BspEI T'CCGG_A Cuts at: 6210 6210 Size: 6547 BspHI T'CATG_A Cuts at: 4436 6166 4436 Size: 1730 4817 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2641 3902 844 Size: 1797 1261 3489 Fragments arranged by size: 3489 1797 1261 BsrBI CCG'CTC Cuts at: 1394 1823 4434 1394 Size: 429 2611 3507 Fragments arranged by size: 3507 2611 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3438 5042 5216 65 Size: 1183 2190 1604 174 1396 Fragments arranged by size: 2190 1604 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5191 5378 1700 Size: 110 3381 187 2869 Fragments arranged by size: 3381 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6547 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6547 BssSI C'ACGA_G Cuts at: 4607 5619 4607 Size: 1012 5535 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6547 BstBI TT'CG_AA Cuts at: 3688 5351 3688 Size: 1663 4884 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6547 BstXI CCAn_nnnn'nTGG Cuts at: 2857 3076 2857 Size: 219 6328 BstYI r'GATC_y Cuts at: 1972 2341 3877 4244 4629 4646 6098 6184 6196 Size: 369 1536 367 385 17 1452 86 12 Cuts at: 6196 6542 1972 Size: 346 1977 Fragments arranged by size: 1977 1536 1452 385 369 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5995 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5808 Fragments arranged by size: 5808 683 56 BtsI GCAGTG_nn' Cuts at: 4063 4857 4877 4063 Size: 794 20 5733 Fragments arranged by size: 5733 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6547 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6512 DraI TTT'AAA Cuts at: 3574 3851 3574 Size: 277 6270 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5554 809 Size: 507 4238 1802 Fragments arranged by size: 4238 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3994 4883 6512 Size: 54 1425 50 275 18 2164 889 1629 Cuts at: 6512 8 Size: 43 Fragments arranged by size: 2164 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3994 1537 Size: 275 2182 4090 Fragments arranged by size: 4090 2182 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2540 4477 1353 Size: 1187 1937 3423 Fragments arranged by size: 3423 1937 1187 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5134 5508 5654 387 Size: 1064 138 3545 374 146 1280 Fragments arranged by size: 3545 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2020 2342 2390 2421 2561 3122 3320 Size: 393 388 322 48 31 140 561 198 Cuts at: 3320 3567 3687 4610 5207 5994 6262 1239 Size: 247 120 923 597 787 268 1524 Fragments arranged by size: 1524 923 787 597 561 393 388 322 268 247 198 140 120 48 31 EcoO109I rG'GnC_Cy Cuts at: 2532 3190 3504 3622 2532 Size: 658 314 118 5457 Fragments arranged by size: 5457 658 314 118 EcoRI G'AATT_C Cuts at: 3856 4234 3856 Size: 378 6169 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2719 3728 2719 Size: 1009 5538 FspI TGC'GCA Cuts at: 5053 5053 Size: 6547 HaeII r_GCGC'y Cuts at: 1678 5694 1678 Size: 4016 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2541 2561 2573 2593 5188 Size: 32 135 32 969 20 12 20 2595 Cuts at: 5188 5220 5262 5294 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2595 969 135 42 32 32 32 32 20 20 12 HincII GTy'rAC Cuts at: 669 1657 1938 3891 4137 669 Size: 988 281 1953 246 3079 Fragments arranged by size: 3079 1953 988 281 246 HindIII A'AGCT_T Cuts at: 748 3238 748 Size: 2490 4057 HpaI GTT'AAC Cuts at: 4137 4137 Size: 6547 KpnI G_GTAC'C Cuts at: 3872 3872 Size: 6547 MfeI C'AATT_G Cuts at: 2302 4146 2302 Size: 1844 4703 MluI A'CGCG_T Cuts at: 6423 6423 Size: 6547 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2848 3468 5661 5845 1305 Size: 1543 620 2193 184 2007 Fragments arranged by size: 2193 2007 1543 620 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4725 Fragments arranged by size: 4725 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2855 4505 4864 5023 518 Size: 648 1689 1650 359 159 2042 Fragments arranged by size: 2042 1689 1650 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3614 3963 4640 5788 6033 1606 Size: 2008 349 677 1148 245 2120 Fragments arranged by size: 2120 2008 1148 677 349 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5995 NdeI CA'TA_TG Cuts at: 387 387 Size: 6547 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6547 NotI GC'GGCC_GC Cuts at: 3994 3994 Size: 6547 NspI r_CATG'y Cuts at: 1132 1229 3905 5450 1132 Size: 97 2676 1545 2229 Fragments arranged by size: 2676 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6547 PciI A'CATG_T Cuts at: 5446 5446 Size: 6547 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6547 PfoI T'CCnGG_A Cuts at: 2538 2538 Size: 6547 PmeI GTTT'AAAC Cuts at: 3851 3851 Size: 6547 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4616 4648 6129 6161 4616 Size: 32 1481 32 5002 Fragments arranged by size: 5002 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3190 3622 3190 Size: 432 6115 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6547 PsiI TTA'TAA Cuts at: 4117 4117 Size: 6547 PspOMI G'GGCC_C Cuts at: 2531 2531 Size: 6547 PstI C_TGCA'G Cuts at: 830 3772 3899 830 Size: 2942 127 3478 Fragments arranged by size: 3478 2942 127 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6547 PvuII CAG'CTG Cuts at: 3614 3614 Size: 6547 SalI G'TCGA_C Cuts at: 1936 3889 1936 Size: 1953 4594 SbfI CC_TGCA'GG Cuts at: 3899 3899 Size: 6547 ScaI AGT'ACT Cuts at: 4795 6507 4795 Size: 1712 4835 SfcI C'TryA_G Cuts at: 826 1045 3725 3768 3839 3895 5030 5711 5902 Size: 219 2680 43 71 56 1135 681 191 Cuts at: 5902 826 Size: 1471 Fragments arranged by size: 2680 1471 1135 681 219 191 71 56 43 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6547 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6547 SmaI CCC'GGG Cuts at: 1872 3874 1872 Size: 2002 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2393 2789 4651 5552 5814 820 Size: 197 926 450 396 1862 901 262 1553 Fragments arranged by size: 1862 1553 926 901 450 396 262 197 SnaBI TAC'GTA Cuts at: 493 4338 493 Size: 3845 2702 SpeI A'CTAG_T Cuts at: 152 2837 152 Size: 2685 3862 SphI G_CATG'C Cuts at: 1132 3905 1132 Size: 2773 3774 SspI AAT'ATT Cuts at: 5 52 2527 4471 5 Size: 47 2475 1944 2081 Fragments arranged by size: 2475 2081 1944 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6547 StyI C'CwwG_G Cuts at: 513 1065 2620 2703 3528 3986 513 Size: 552 1555 83 825 458 3074 Fragments arranged by size: 3074 1555 825 552 458 83 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4574 4591 4744 4929 5374 325 Size: 1255 2994 17 153 185 445 1498 Fragments arranged by size: 2994 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4793 6505 96 Size: 274 80 33 51 1443 2816 1712 138 Fragments arranged by size: 2816 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6507 6539 6507 Size: 32 6515 Tth111I GACn'n_nGTC Cuts at: 1316 3620 1316 Size: 2304 4243 XbaI T'CTAG_A Cuts at: 3443 3883 3443 Size: 440 6107 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2024 3151 3662 2024 Size: 1127 511 4909 Fragments arranged by size: 4909 1127 511 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6547 XmaI C'CCGG_G Cuts at: 1870 3872 1870 Size: 2002 4545 XmnI GAAnn'nnTTC Cuts at: 2012 4676 2012 Size: 2664 3883 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4355 276 Size: 53 83 186 936 2821 2468 Fragments arranged by size: 2821 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI