(Circular) (Six-base) MAPSORT of: pFN21AB4636.seq Check: 136 from: 1 to: 8083 pFN21AB4636 8083 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2776 3057 3448 3997 2776 Size: 281 391 549 6862 Fragments arranged by size: 6862 549 391 281 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5893 278 Size: 53 83 186 936 4357 2468 Fragments arranged by size: 4357 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5426 1937 Size: 3489 4594 Acc65I G'GTAC_C Cuts at: 3581 5404 3581 Size: 1823 6260 AclI AA'CG_TT Cuts at: 1563 6210 6583 1563 Size: 4647 373 3063 Fragments arranged by size: 4647 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2204 3785 4736 4980 6146 7530 7798 1632 Size: 572 1581 951 244 1166 1384 268 1917 Fragments arranged by size: 1917 1581 1384 1166 951 572 268 244 AfeI AGC'GCT Cuts at: 2873 5345 2873 Size: 2472 5611 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7886 AflIII A'CryG_T Cuts at: 1203 6982 7959 1203 Size: 5779 977 1327 Fragments arranged by size: 5779 1327 977 AgeI A'CCGG_T Cuts at: 6914 6914 Size: 8083 AhdI GACnn_n'nnGTC Cuts at: 6812 6812 Size: 8083 AleI CACnn'nnGTG Cuts at: 1166 3333 1166 Size: 2167 5916 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2160 5162 1184 Size: 976 3002 4105 Fragments arranged by size: 4105 3002 976 AlwNI CAG_nnn'CTG Cuts at: 2842 3554 3689 3817 3851 4913 5183 5826 7398 Size: 712 135 128 34 1062 270 643 1572 Cuts at: 7398 2842 Size: 3527 Fragments arranged by size: 3527 1572 1062 712 643 270 135 128 34 ApaI G_GGCC'C Cuts at: 2110 2571 2762 3546 4228 4576 5162 2110 Size: 461 191 784 682 348 586 5031 Fragments arranged by size: 5031 784 682 586 461 348 191 ApaLI G'TGCA_C Cuts at: 6140 7296 6140 Size: 1156 6927 ApoI r'AATT_y Cuts at: 1493 4505 4680 5392 5618 5770 7879 1493 Size: 3012 175 712 226 152 2109 1697 Fragments arranged by size: 3012 2109 1697 712 226 175 152 AseI AT'TA_AT Cuts at: 160 1032 6637 160 Size: 872 5605 1606 Fragments arranged by size: 5605 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8083 AvaI C'yCGr_G Cuts at: 1870 1943 2355 2490 2676 3307 3486 4140 4941 Size: 73 412 135 186 631 179 654 801 Cuts at: 4941 4974 5231 5408 1870 Size: 33 257 177 4545 Fragments arranged by size: 4545 801 654 631 412 257 186 179 177 135 73 33 BamHI G'GATC_C Cuts at: 2339 4190 5413 5780 2339 Size: 1851 1223 367 4642 Fragments arranged by size: 4642 1851 1223 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3190 3460 3569 3581 3713 Size: 325 217 627 1403 270 109 12 132 Cuts at: 3713 5257 5404 6859 618 Size: 1544 147 1455 1842 Fragments arranged by size: 1842 1544 1455 1403 627 325 270 217 147 132 109 12 BanII G_rGCy'C Cuts at: 1391 2110 2269 2490 2571 2639 2685 2736 2762 Size: 719 159 221 81 68 46 51 26 Cuts at: 2762 3268 3546 4130 4141 4228 4383 4576 4973 Size: 506 278 584 11 87 155 193 397 Cuts at: 4973 5162 5215 5231 5402 1391 Size: 189 53 16 171 4072 Fragments arranged by size: 4072 719 584 506 397 278 221 193 189 171 159 155 87 81 68 53 51 46 26 16 11 BbeI G_GCGC'C Cuts at: 3573 3573 Size: 8083 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2075 3225 5328 928 Size: 971 176 1150 2103 3683 Fragments arranged by size: 3683 2103 1150 971 176 BbvCI CC'TCA_GC Cuts at: 2320 3253 3258 4887 2320 Size: 933 5 1629 5516 Fragments arranged by size: 5516 1629 933 5 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6274 6308 1903 Size: 34 4337 34 3678 Fragments arranged by size: 4337 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5975 7185 5975 Size: 1210 6873 BclI T'GATC_A Cuts at: 1548 2520 2952 4419 1548 Size: 972 432 1467 5212 Fragments arranged by size: 5212 1467 972 432 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3596 3841 5363 Size: 107 122 71 631 605 1924 245 1522 Cuts at: 5363 6694 136 Size: 1331 1525 Fragments arranged by size: 1924 1525 1522 1331 631 605 245 122 107 71 BglII A'GATC_T Cuts at: 8078 8078 Size: 8083 BlpI GC'TnA_GC Cuts at: 2511 4112 5500 2511 Size: 1601 1388 5094 Fragments arranged by size: 5094 1601 1388 Bme1580I G_kGCm'C Cuts at: 1790 2110 2571 2716 2762 3546 4228 4576 5162 Size: 320 461 145 46 784 682 348 586 Cuts at: 5162 6144 7300 1790 Size: 982 1156 2573 Fragments arranged by size: 2573 1156 982 784 682 586 461 348 320 145 46 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8083 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3542 4383 5814 Size: 416 53 83 399 24 2125 841 1431 Cuts at: 5814 6772 442 Size: 958 1753 Fragments arranged by size: 2125 1753 1431 958 841 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8083 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4987 4987 Size: 8083 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2810 2840 2864 3080 3558 3873 4071 4233 Size: 1571 30 24 216 478 315 198 162 Cuts at: 4233 4313 4604 4784 5273 6743 1239 Size: 80 291 180 489 1470 2579 Fragments arranged by size: 2579 1571 1470 489 478 315 291 216 198 180 162 80 30 24 Bpu10I CC'TnA_GC Cuts at: 2320 3253 3258 3654 4881 4887 5199 5240 2320 Size: 933 5 396 1227 6 312 41 5163 Fragments arranged by size: 5163 1227 933 396 312 41 6 5 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2653 4085 6208 7073 7371 2653 Size: 1432 2123 865 298 3365 Fragments arranged by size: 3365 2123 1432 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6746 882 Size: 617 5247 2219 Fragments arranged by size: 5247 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5874 493 Size: 711 4670 2702 Fragments arranged by size: 4670 2702 711 BsaBI GATnn'nnATC Cuts at: 8077 8077 Size: 8083 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3570 5890 6272 6907 Size: 53 83 186 936 2037 2320 382 635 Cuts at: 6907 275 Size: 1451 Fragments arranged by size: 2320 2037 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2382 3318 4078 4398 4491 5292 6516 6914 7188 Size: 936 760 320 93 801 1224 398 274 Cuts at: 7188 7335 7746 2382 Size: 147 411 2719 Fragments arranged by size: 2719 1224 936 801 760 411 398 320 274 147 93 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3940 3970 4311 4341 1178 Size: 30 165 30 2537 30 341 30 4920 Fragments arranged by size: 4920 2537 341 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2054 2259 2262 2577 2720 2951 3501 3963 Size: 868 205 3 315 143 231 550 462 Cuts at: 3963 4856 1186 Size: 893 4413 Fragments arranged by size: 4413 893 868 550 462 315 231 205 143 3 BseYI C'CCAG_C Cuts at: 2716 2746 2752 2983 3762 4123 4335 4827 4906 Size: 30 6 231 779 361 212 492 79 Cuts at: 4906 5163 5340 5358 7286 7782 2716 Size: 257 177 18 1928 496 3017 Fragments arranged by size: 3017 1928 779 496 492 361 257 231 212 177 79 30 18 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3016 4121 4484 4613 1163 Size: 573 1280 1105 363 129 4633 Fragments arranged by size: 4633 1280 1105 573 363 129 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5533 6294 7322 1540 Size: 275 186 3532 761 1028 2301 Fragments arranged by size: 3532 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2139 2639 2736 2826 3096 3409 4113 4770 5215 Size: 500 97 90 270 313 704 657 445 Cuts at: 5215 6144 6229 7300 2139 Size: 929 85 1071 2922 Fragments arranged by size: 2922 1071 929 704 657 500 445 313 270 97 90 85 BsmI GAATG_Cn' Cuts at: 2392 4616 4903 5594 5687 2392 Size: 2224 287 691 93 4788 Fragments arranged by size: 4788 2224 691 287 93 BsmBI CGTCTCn'nnnn_ Cuts at: 5128 5128 Size: 8083 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2110 2139 2269 2490 2571 2639 2685 Size: 399 320 29 130 221 81 68 46 Cuts at: 2685 2716 2736 2762 2826 3096 3268 3409 3546 Size: 31 20 26 64 270 172 141 137 Cuts at: 3546 4113 4130 4141 4228 4383 4576 4770 4973 Size: 567 17 11 87 155 193 194 203 Cuts at: 4973 5162 5215 5231 5402 6144 6229 7300 1391 Size: 189 53 16 171 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 567 399 320 270 221 203 194 193 189 172 171 155 141 137 130 87 85 81 68 64 53 46 31 29 26 20 17 16 11 BspEI T'CCGG_A Cuts at: 3318 4398 4491 7746 3318 Size: 1080 93 3255 3655 Fragments arranged by size: 3655 3255 1080 93 BspHI T'CATG_A Cuts at: 2377 3351 5972 7702 2377 Size: 974 2621 1730 2758 Fragments arranged by size: 2758 2621 1730 974 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2776 3021 3057 3448 3834 3997 4456 4635 Size: 1932 245 36 391 386 163 459 179 Cuts at: 4635 5438 844 Size: 803 3489 Fragments arranged by size: 3489 1932 803 459 391 386 245 179 163 36 BsrBI CCG'CTC Cuts at: 1394 1823 3612 3746 5970 1394 Size: 429 1789 134 2224 3507 Fragments arranged by size: 3507 2224 1789 429 134 BsrDI GCAATG_nn' Cuts at: 65 1248 2139 4530 4549 6578 6752 65 Size: 1183 891 2391 19 2029 174 1396 Fragments arranged by size: 2391 2029 1396 1183 891 174 19 BsrFI r'CCGG_y Cuts at: 1700 1810 2605 3572 6727 6914 1700 Size: 110 795 967 3155 187 2869 Fragments arranged by size: 3155 2869 967 795 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8083 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8083 BssSI C'ACGA_G Cuts at: 2924 4695 6143 7155 2924 Size: 1771 1448 1012 3852 Fragments arranged by size: 3852 1771 1448 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4009 4108 4546 794 Size: 3215 99 438 4331 Fragments arranged by size: 4331 3215 438 99 BstBI TT'CG_AA Cuts at: 6887 6887 Size: 8083 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8083 BstXI CCAn_nnnn'nTGG Cuts at: 3088 3886 4738 3088 Size: 798 852 6433 Fragments arranged by size: 6433 852 798 BstYI r'GATC_y Cuts at: 1972 2339 4190 5413 5780 6165 6182 7634 7720 Size: 367 1851 1223 367 385 17 1452 86 Cuts at: 7720 7732 8078 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1851 1452 1223 385 367 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4839 4962 4839 Size: 123 7960 BtgI C'CryG_G Cuts at: 513 1065 2004 2685 2904 3087 3222 3345 3789 Size: 552 939 681 219 183 135 123 444 Cuts at: 3789 4300 513 Size: 511 4296 Fragments arranged by size: 4296 939 681 552 511 444 219 183 135 123 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3905 487 Size: 683 56 2679 4665 Fragments arranged by size: 4665 2679 683 56 BtsI GCAGTG_nn' Cuts at: 3560 3693 4159 4630 5599 6393 6413 3560 Size: 133 466 471 969 794 20 5230 Fragments arranged by size: 5230 969 794 471 466 133 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8083 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3471 3506 529 Size: 35 2907 35 5106 Fragments arranged by size: 5106 2907 35 35 DraI TTT'AAA Cuts at: 5387 5387 Size: 8083 DraIII CAC_nnn'GTG Cuts at: 2257 2257 Size: 8083 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3432 7090 809 Size: 507 2116 3658 1802 Fragments arranged by size: 3658 2116 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3439 4675 5530 Size: 54 1425 50 275 18 1609 1236 855 Cuts at: 5530 6419 8048 8 Size: 889 1629 43 Fragments arranged by size: 1629 1609 1425 1236 889 855 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5530 1537 Size: 275 3718 4090 Fragments arranged by size: 4090 3718 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4260 4428 4923 6013 1353 Size: 2907 168 495 1090 3423 Fragments arranged by size: 3423 2907 1090 495 168 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6670 7044 7190 387 Size: 1064 138 5081 374 146 1280 Fragments arranged by size: 5081 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2637 2734 5213 2637 Size: 97 2479 5507 Fragments arranged by size: 5507 2479 97 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2204 2810 2840 2864 3080 3558 3785 Size: 393 572 606 30 24 216 478 227 Cuts at: 3785 3873 4071 4233 4313 4604 4736 4784 4980 Size: 88 198 162 80 291 132 48 196 Cuts at: 4980 5273 6146 6743 7530 7798 1239 Size: 293 873 597 787 268 1524 Fragments arranged by size: 1524 873 787 606 597 572 478 393 293 291 268 227 216 198 196 162 132 88 80 48 30 24 EcoO109I rG'GnC_Cy Cuts at: 2106 2360 2367 2470 2567 2568 3425 3542 3543 Size: 254 7 103 97 1 857 117 1 Cuts at: 3543 3660 3755 3833 4224 4225 4242 4572 4781 Size: 117 95 78 391 1 17 330 209 Cuts at: 4781 5041 5158 5159 5248 2106 Size: 260 117 1 89 4941 Fragments arranged by size: 4941 857 391 330 260 254 209 117 117 117 103 97 95 89 78 17 7 1 1 1 1 EcoRI G'AATT_C Cuts at: 4680 5392 5770 4680 Size: 712 378 6993 Fragments arranged by size: 6993 712 378 FspI TGC'GCA Cuts at: 4539 6589 4539 Size: 2050 6033 HaeII r_GCGC'y Cuts at: 1678 2875 3573 5347 7230 1678 Size: 1197 698 1774 1883 2531 Fragments arranged by size: 2531 1883 1774 1197 698 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2542 2574 3940 3972 4058 Size: 32 135 32 970 32 1366 32 86 Cuts at: 4058 4090 4922 4954 6724 6756 6798 6830 1373 Size: 32 832 32 1770 32 42 32 2626 Fragments arranged by size: 2626 1770 1366 970 832 135 86 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5427 5673 669 Size: 988 281 3489 246 3079 Fragments arranged by size: 3489 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 2216 748 Size: 1468 6615 HpaI GTT'AAC Cuts at: 5673 5673 Size: 8083 KasI G'GCGC_C Cuts at: 3569 3569 Size: 8083 KpnI G_GTAC'C Cuts at: 3585 5408 3585 Size: 1823 6260 MfeI C'AATT_G Cuts at: 5682 5682 Size: 8083 MluI A'CGCG_T Cuts at: 7959 7959 Size: 8083 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2389 3506 7197 7381 1305 Size: 1084 1117 3691 184 2007 Fragments arranged by size: 3691 2007 1117 1084 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3441 10 Size: 54 1425 343 1609 4652 Fragments arranged by size: 4652 1609 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2459 3051 3086 3333 3884 4400 4736 Size: 648 1293 592 35 247 551 516 336 Cuts at: 4736 6041 6400 6559 518 Size: 1305 359 159 2042 Fragments arranged by size: 2042 1305 1293 648 592 551 516 359 336 247 159 35 MspA1I CmG'CkG Cuts at: 1606 2033 2756 2839 2947 3257 3737 4339 4891 Size: 427 723 83 108 310 480 602 552 Cuts at: 4891 5000 5021 5074 5167 5362 5499 6176 7324 Size: 109 21 53 93 195 137 677 1148 Cuts at: 7324 7569 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 723 677 602 552 480 427 310 245 195 137 109 108 93 83 53 21 NarI GG'CG_CC Cuts at: 3570 3570 Size: 8083 NcoI C'CATG_G Cuts at: 513 1065 2004 2685 3087 3789 4300 513 Size: 552 939 681 402 702 511 4296 Fragments arranged by size: 4296 939 702 681 552 511 402 NdeI CA'TA_TG Cuts at: 387 387 Size: 8083 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8083 NotI GC'GGCC_GC Cuts at: 5530 5530 Size: 8083 NspI r_CATG'y Cuts at: 1132 1229 3056 4544 4774 5441 6986 1132 Size: 97 1827 1488 230 667 1545 2229 Fragments arranged by size: 2229 1827 1545 1488 667 230 97 PasI CC'CwG_GG Cuts at: 1348 3421 3448 4132 1348 Size: 2073 27 684 5299 Fragments arranged by size: 5299 2073 684 27 PciI A'CATG_T Cuts at: 6982 6982 Size: 8083 PflMI CCAn_nnn'nTGG Cuts at: 1108 2257 3337 3448 3554 3851 4913 1108 Size: 1149 1080 111 106 297 1062 4278 Fragments arranged by size: 4278 1149 1080 1062 297 111 106 PfoI T'CCnGG_A Cuts at: 2546 2826 3535 4048 2546 Size: 280 709 513 6581 Fragments arranged by size: 6581 709 513 280 PmeI GTTT'AAAC Cuts at: 5387 5387 Size: 8083 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6152 6184 7665 7697 6152 Size: 32 1481 32 6538 Fragments arranged by size: 6538 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3425 3660 5041 5248 3425 Size: 235 1381 207 6260 Fragments arranged by size: 6260 1381 235 207 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8083 PsiI TTA'TAA Cuts at: 5653 5653 Size: 8083 PspOMI G'GGCC_C Cuts at: 2106 2567 2758 3542 4224 4572 5158 2106 Size: 461 191 784 682 348 586 5031 Fragments arranged by size: 5031 784 682 586 461 348 191 PstI C_TGCA'G Cuts at: 830 3567 3841 5026 5319 5435 830 Size: 2737 274 1185 293 116 3478 Fragments arranged by size: 3478 2737 1185 293 274 116 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8083 PvuII CAG'CTG Cuts at: 3257 3737 4339 5000 5021 5167 3257 Size: 480 602 661 21 146 6173 Fragments arranged by size: 6173 661 602 480 146 21 SacI G_AGCT'C Cuts at: 2639 2736 5215 2639 Size: 97 2479 5507 Fragments arranged by size: 5507 2479 97 SalI G'TCGA_C Cuts at: 1936 5425 1936 Size: 3489 4594 SanDI GG'GwC_CC Cuts at: 3425 3660 5041 3425 Size: 235 1381 6467 Fragments arranged by size: 6467 1381 235 SbfI CC_TGCA'GG Cuts at: 3841 5435 3841 Size: 1594 6489 ScaI AGT'ACT Cuts at: 6331 8043 6331 Size: 1712 6371 SexAI A'CCwGG_T Cuts at: 2253 2253 Size: 8083 SfcI C'TryA_G Cuts at: 826 1045 2834 3563 3837 4776 5022 5278 5315 Size: 219 1789 729 274 939 246 256 37 Cuts at: 5315 5332 5431 6566 7247 7438 826 Size: 17 99 1135 681 191 1471 Fragments arranged by size: 1789 1471 1135 939 729 681 274 256 246 219 191 99 37 17 SfiI GGCCn_nnn'nGGCC Cuts at: 3841 3841 Size: 8083 SfoI GGC'GCC Cuts at: 3571 3571 Size: 8083 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8083 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8083 SmaI CCC'GGG Cuts at: 1872 2492 2678 3488 4976 5410 1872 Size: 620 186 810 1488 434 4545 Fragments arranged by size: 4545 1488 810 620 434 186 SmlI C'TyrA_G Cuts at: 820 1017 1943 2632 4064 6187 7088 7350 820 Size: 197 926 689 1432 2123 901 262 1553 Fragments arranged by size: 2123 1553 1432 926 901 689 262 197 SnaBI TAC'GTA Cuts at: 493 5874 493 Size: 5381 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8083 SphI G_CATG'C Cuts at: 1132 5441 1132 Size: 4309 3774 SspI AAT'ATT Cuts at: 5 52 6007 5 Size: 47 5955 2081 Fragments arranged by size: 5955 2081 47 StuI AGG'CCT Cuts at: 1517 3215 4056 4602 1517 Size: 1698 841 546 4998 Fragments arranged by size: 4998 1698 841 546 StyI C'CwwG_G Cuts at: 513 1065 2004 2073 2685 3087 3789 3916 4228 Size: 552 939 69 612 402 702 127 312 Cuts at: 4228 4300 4458 4597 4713 5012 5522 513 Size: 72 158 139 116 299 510 3074 Fragments arranged by size: 3074 939 702 612 552 510 402 312 299 158 139 127 116 72 69 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2803 2879 3787 4358 4402 6110 6127 Size: 1255 1223 76 908 571 44 1708 17 Cuts at: 6127 6280 6465 6910 325 Size: 153 185 445 1498 Fragments arranged by size: 1708 1498 1255 1223 908 571 445 185 153 76 44 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6329 8041 96 Size: 274 80 33 51 1443 4352 1712 138 Fragments arranged by size: 4352 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3123 3155 3444 3476 8043 8075 3123 Size: 32 289 32 4567 32 3131 Fragments arranged by size: 4567 3131 289 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2311 1316 Size: 995 7088 XbaI T'CTAG_A Cuts at: 5419 5419 Size: 8083 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2032 3418 3592 3883 2032 Size: 1386 174 291 6232 Fragments arranged by size: 6232 1386 291 174 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8083 XmaI C'CCGG_G Cuts at: 1870 2490 2676 3486 4974 5408 1870 Size: 620 186 810 1488 434 4545 Fragments arranged by size: 4545 1488 810 620 434 186 XmnI GAAnn'nnTTC Cuts at: 6212 6212 Size: 8083 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5891 276 Size: 53 83 186 936 4357 2468 Fragments arranged by size: 4357 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SapI SrfI SwaI