Restriction map for
pFN21AB4596
(Linear) (Six-base) MAPSORT of: KIBB4596.seq Check: 9566 from: 1 to: 2751
KIBB4596.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2443 2751
Size: 2443 308
AccI GT'mk_AC
Cuts at: 0 1235 2751
Size: 1235 1516
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 111 2347 2751
Size: 61 50 2236 404
Fragments arranged by size:
2236 404 61 50
AflIII A'CryG_T
Cuts at: 0 2660 2751
Size: 2660 91
AleI CACnn'nnGTG
Cuts at: 0 362 2496 2716 2751
Size: 362 2134 220 35
Fragments arranged by size:
2134 362 220 35
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1532 1896 2751
Size: 1532 364 855
Fragments arranged by size:
1532 855 364
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 899 931 2751
Size: 899 32 1820
Fragments arranged by size:
1820 899 32
AlwNI CAG_nnn'CTG
Cuts at: 0 18 2751
Size: 18 2733
ApaLI G'TGCA_C
Cuts at: 0 355 2751
Size: 355 2396
ApoI r'AATT_y
Cuts at: 0 272 1936 2751
Size: 272 1664 815
Fragments arranged by size:
1664 815 272
AvaI C'yCGr_G
Cuts at: 0 155 989 2333 2751
Size: 155 834 1344 418
Fragments arranged by size:
1344 834 418 155
BamHI G'GATC_C
Cuts at: 0 850 2751
Size: 850 1901
BanI G'GyrC_C
Cuts at: 0 634 697 1171 1302 1978 2751
Size: 634 63 474 131 676 773
Fragments arranged by size:
773 676 634 474 131 63
BanII G_rGCy'C
Cuts at: 0 2009 2751
Size: 2009 742
BbeI G_GCGC'C
Cuts at: 0 1175 2751
Size: 1175 1576
BbsI GAAGACnn'nnnn_
Cuts at: 0 254 818 2335 2751
Size: 254 564 1517 416
Fragments arranged by size:
1517 564 416 254
BbvCI CC'TCA_GC
Cuts at: 0 1495 2751
Size: 1495 1256
BciVI GTATCCnnnnn_n'
Cuts at: 0 2458 2751
Size: 2458 293
BclI T'GATC_A
Cuts at: 0 381 503 683 1725 1847 2231 2751
Size: 381 122 180 1042 122 384 520
Fragments arranged by size:
1042 520 384 381 180 122 122
BglII A'GATC_T
Cuts at: 0 1673 2312 2751
Size: 1673 639 439
Fragments arranged by size:
1673 639 439
BlpI GC'TnA_GC
Cuts at: 0 1143 2751
Size: 1143 1608
Bme1580I G_kGCm'C
Cuts at: 0 359 637 1207 1708 1981 2751
Size: 359 278 570 501 273 770
Fragments arranged by size:
770 570 501 359 278 273
BmgBI CAC'GTC
Cuts at: 0 1950 2751
Size: 1950 801
BmrI ACTGGGnnnn_n'
Cuts at: 0 1177 1718 2751
Size: 1177 541 1033
Fragments arranged by size:
1177 1033 541
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1474 2377 2395 2598 2751
Size: 1474 903 18 203 153
Fragments arranged by size:
1474 903 203 153 18
Bpu10I CC'TnA_GC
Cuts at: 0 1495 2751
Size: 1495 1256
BsaI GGTCTCn'nnnn_
Cuts at: 0 1408 2341 2751
Size: 1408 933 410
Fragments arranged by size:
1408 933 410
BsaAI yAC'GTr
Cuts at: 0 1853 2751
Size: 1853 898
BsaBI GATnn'nnATC
Cuts at: 0 164 766 2110 2751
Size: 164 602 1344 641
Fragments arranged by size:
1344 641 602 164
BsaHI Gr'CG_yC
Cuts at: 0 1172 2751
Size: 1172 1579
BsaWI w'CCGG_w
Cuts at: 0 818 1547 2751
Size: 818 729 1204
Fragments arranged by size:
1204 818 729
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 536 566 750 780 899 929 1720 1750
Size: 536 30 184 30 119 30 791 30
Cuts at: 1750 2751
Size: 1001
Fragments arranged by size:
1001 791 536 184 119 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1318 1426 2017 2077 2751
Size: 1318 108 591 60 674
Fragments arranged by size:
1318 674 591 108 60
BseYI C'CCAG_C
Cuts at: 0 1488 2206 2751
Size: 1488 718 545
Fragments arranged by size:
1488 718 545
BsiEI CG_ry'CG
Cuts at: 0 2717 2751
Size: 2717 34
BsiHKAI G_wGCw'C
Cuts at: 0 359 2751
Size: 359 2392
BsmBI CGTCTCn'nnnn_
Cuts at: 0 743 788 2314 2751
Size: 743 45 1526 437
Fragments arranged by size:
1526 743 437 45
Bsp1286I G_dGCh'C
Cuts at: 0 359 637 1207 1708 1981 2009 2751
Size: 359 278 570 501 273 28 742
Fragments arranged by size:
742 570 501 359 278 273 28
BspEI T'CCGG_A
Cuts at: 0 818 1547 2751
Size: 818 729 1204
Fragments arranged by size:
1204 818 729
BspHI T'CATG_A
Cuts at: 0 116 294 2751
Size: 116 178 2457
Fragments arranged by size:
2457 178 116
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2443 2751
Size: 2443 308
BsrBI CCG'CTC
Cuts at: 0 533 2751
Size: 533 2218
BsrDI GCAATG_nn'
Cuts at: 0 239 2052 2751
Size: 239 1813 699
Fragments arranged by size:
1813 699 239
BsrFI r'CCGG_y
Cuts at: 0 1400 2038 2751
Size: 1400 638 713
Fragments arranged by size:
1400 713 638
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2051 2751
Size: 2051 700
BstBI TT'CG_AA
Cuts at: 0 270 2751
Size: 270 2481
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1155 2751
Size: 1155 1596
BstYI r'GATC_y
Cuts at: 0 249 850 1240 1593 1673 2312 2531 2751
Size: 249 601 390 353 80 639 219 220
Fragments arranged by size:
639 601 390 353 249 220 219 80
BtgI C'CryG_G
Cuts at: 0 1223 1361 1520 2717 2751
Size: 1223 138 159 1197 34
Fragments arranged by size:
1223 1197 159 138 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2453 2751
Size: 2453 298
BtsI GCAGTG_nn'
Cuts at: 0 369 2386 2751
Size: 369 2017 365
Fragments arranged by size:
2017 369 365
DraI TTT'AAA
Cuts at: 0 525 2751
Size: 525 2226
DrdI GACnn_nn'nnGTC
Cuts at: 0 2555 2751
Size: 2555 196
EaeI y'GGCC_r
Cuts at: 0 938 1388 2714 2751
Size: 938 450 1326 37
Fragments arranged by size:
1326 938 450 37
EagI C'GGCC_G
Cuts at: 0 2714 2751
Size: 2714 37
EarI CTCTTCn'nnn_
Cuts at: 0 1459 1709 2305 2751
Size: 1459 250 596 446
Fragments arranged by size:
1459 596 446 250
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 981 2751
Size: 981 1770
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 111 1474 2347 2377 2395 2598 2751
Size: 61 50 1363 873 30 18 203 153
Fragments arranged by size:
1363 873 203 153 61 50 30 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1908 2677 2751
Size: 1908 769 74
Fragments arranged by size:
1908 769 74
EcoO109I rG'GnC_Cy
Cuts at: 0 948 1777 2129 2751
Size: 948 829 352 622
Fragments arranged by size:
948 829 622 352
EcoRI G'AATT_C
Cuts at: 0 272 2751
Size: 272 2479
EcoRV GAT'ATC
Cuts at: 0 114 2160 2751
Size: 114 2046 591
Fragments arranged by size:
2046 591 114
HaeII r_GCGC'y
Cuts at: 0 1175 1299 2751
Size: 1175 124 1452
Fragments arranged by size:
1452 1175 124
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 787 819 835 867 1090 1122 1407 1439
Size: 787 32 16 32 223 32 285 32
Cuts at: 1439 2297 2329 2751
Size: 858 32 422
Fragments arranged by size:
858 787 422 285 223 32 32 32
32 32 16
HincII GTy'rAC
Cuts at: 0 1236 1965 2751
Size: 1236 729 786
Fragments arranged by size:
1236 786 729
HindIII A'AGCT_T
Cuts at: 0 2596 2751
Size: 2596 155
KasI G'GCGC_C
Cuts at: 0 1171 2751
Size: 1171 1580
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 2001 2751
Size: 193 1808 750
Fragments arranged by size:
1808 750 193
MscI TGG'CCA
Cuts at: 0 940 2751
Size: 940 1811
MslI CAynn'nnrTG
Cuts at: 0 362 640 1514 1984 2116 2496 2652 2716
Size: 362 278 874 470 132 380 156 64
Cuts at: 2716 2751
Size: 35
Fragments arranged by size:
874 470 380 362 278 156 132 64
35
MspA1I CmG'CkG
Cuts at: 0 873 1346 2496 2690 2751
Size: 873 473 1150 194 61
Fragments arranged by size:
1150 873 473 194 61
NarI GG'CG_CC
Cuts at: 0 1172 2751
Size: 1172 1579
NcoI C'CATG_G
Cuts at: 0 1361 2751
Size: 1361 1390
NspI r_CATG'y
Cuts at: 0 900 1449 2751
Size: 900 549 1302
Fragments arranged by size:
1302 900 549
PfoI T'CCnGG_A
Cuts at: 0 2479 2751
Size: 2479 272
PmlI CAC'GTG
Cuts at: 0 1853 2751
Size: 1853 898
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 899 931 2751
Size: 899 32 1820
Fragments arranged by size:
1820 899 32
PvuII CAG'CTG
Cuts at: 0 873 2496 2751
Size: 873 1623 255
Fragments arranged by size:
1623 873 255
SalI G'TCGA_C
Cuts at: 0 1234 2751
Size: 1234 1517
SapI GCTCTTCn'nnn_
Cuts at: 0 1709 2751
Size: 1709 1042
SexAI A'CCwGG_T
Cuts at: 0 50 2751
Size: 50 2701
SfoI GGC'GCC
Cuts at: 0 1173 2751
Size: 1173 1578
SmaI CCC'GGG
Cuts at: 0 157 2335 2751
Size: 157 2178 416
Fragments arranged by size:
2178 416 157
SspI AAT'ATT
Cuts at: 0 2194 2751
Size: 2194 557
StuI AGG'CCT
Cuts at: 0 1043 2751
Size: 1043 1708
StyI C'CwwG_G
Cuts at: 0 941 1361 2751
Size: 941 420 1390
Fragments arranged by size:
1390 941 420
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1224 1274 1582 2239 2393 2751
Size: 1224 50 308 657 154 358
Fragments arranged by size:
1224 657 358 308 154 50
TatI w'GTAC_w
Cuts at: 0 899 2751
Size: 899 1852
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 307 339 536 568 1720 1752 2131 2163
Size: 307 32 197 32 1152 32 379 32
Cuts at: 2163 2751
Size: 588
Fragments arranged by size:
1152 588 379 307 197 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 52 1102 2751
Size: 52 1050 1649
Fragments arranged by size:
1649 1050 52
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1788 2751
Size: 1788 963
XmaI C'CCGG_G
Cuts at: 0 155 2333 2751
Size: 155 2178 418
Fragments arranged by size:
2178 418 155
XmnI GAAnn'nnTTC
Cuts at: 0 2293 2751
Size: 2293 458
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlfI AloI AlwNI
ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII Hin4I
HincII HindIII KasI MmeI MscI MslI MspA1I NarI
NcoI NspI PfoI PmlI PpiI PvuII SalI SapI
SexAI SfoI SmaI SspI StuI StyI TaqII TatI
TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI ApaI
AscI AseI AsiSI AvrII BaeI BcgI BfrBI BglI
BmtI BplI BpuEI BsgI BsiWI BsmI BsrGI BssHII
BssSI BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoICRI
FalI FseI FspI FspAI HpaI KpnI MfeI MluI
NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PflMI PmeI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacI SacII SanDI
SbfI ScaI SfcI SfiI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SwaI XbaI XhoI ZraI