(Circular) (Six-base) MAPSORT of: pFN21AB4563.seq Check: 8062 from: 1 to: 7435 pFN21AB4563 7435 bp With 182 enzymes: SgfI * October 20, 2009 10:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4357 4357 Size: 7435 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5245 278 Size: 53 83 186 936 3709 2468 Fragments arranged by size: 3709 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2537 4778 1937 Size: 600 2241 4594 Fragments arranged by size: 4594 2241 600 Acc65I G'GTAC_C Cuts at: 2828 4239 4337 4756 2828 Size: 1411 98 419 5507 Fragments arranged by size: 5507 1411 419 98 AclI AA'CG_TT Cuts at: 1563 5562 5935 1563 Size: 3999 373 3063 Fragments arranged by size: 3999 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2759 2891 3551 3592 3606 3718 4339 5498 Size: 1127 132 660 41 14 112 621 1159 Cuts at: 5498 6882 7150 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1159 1127 660 621 268 132 112 41 14 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7238 AflIII A'CryG_T Cuts at: 1203 3198 4282 6334 7311 1203 Size: 1995 1084 2052 977 1327 Fragments arranged by size: 2052 1995 1327 1084 977 AgeI A'CCGG_T Cuts at: 6266 6266 Size: 7435 AhdI GACnn_n'nnGTC Cuts at: 2048 6164 2048 Size: 4116 3319 AleI CACnn'nnGTG Cuts at: 1166 4364 4503 1166 Size: 3198 139 4098 Fragments arranged by size: 4098 3198 139 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3051 3497 4382 1184 Size: 1867 446 885 4237 Fragments arranged by size: 4237 1867 885 446 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4598 4630 4598 Size: 32 7403 AlwNI CAG_nnn'CTG Cuts at: 3055 3302 3556 4483 5178 6750 3055 Size: 247 254 927 695 1572 3740 Fragments arranged by size: 3740 1572 927 695 254 247 ApaI G_GGCC'C Cuts at: 2635 3982 2635 Size: 1347 6088 ApaLI G'TGCA_C Cuts at: 5492 6648 5492 Size: 1156 6279 ApoI r'AATT_y Cuts at: 1493 2750 3215 4220 4718 4744 4970 5122 7231 Size: 1257 465 1005 498 26 226 152 2109 Cuts at: 7231 1493 Size: 1697 Fragments arranged by size: 2109 1697 1257 1005 498 465 226 152 26 AseI AT'TA_AT Cuts at: 160 1032 5989 160 Size: 872 4957 1606 Fragments arranged by size: 4957 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7435 AvaI C'yCGr_G Cuts at: 1870 1943 2250 2919 3210 4248 4362 4760 1870 Size: 73 307 669 291 1038 114 398 4545 Fragments arranged by size: 4545 1038 669 398 307 291 114 73 BamHI G'GATC_C Cuts at: 4765 5132 4765 Size: 367 7068 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2828 3848 4115 4239 4337 Size: 325 217 627 1041 1020 267 124 98 Cuts at: 4337 4411 4756 6211 618 Size: 74 345 1455 1842 Fragments arranged by size: 1842 1455 1041 1020 627 345 325 267 217 124 98 74 BanII G_rGCy'C Cuts at: 1391 2235 2635 2865 3697 3732 3982 4184 4754 Size: 844 400 230 832 35 250 202 570 Cuts at: 4754 1391 Size: 4072 Fragments arranged by size: 4072 844 832 570 400 250 230 202 35 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3598 4279 928 Size: 971 1699 681 4084 Fragments arranged by size: 4084 1699 971 681 BbvCI CC'TCA_GC Cuts at: 2156 2936 3357 2156 Size: 780 421 6234 Fragments arranged by size: 6234 780 421 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3808 3842 4253 4287 5626 5660 1903 Size: 34 1871 34 411 34 1339 34 3678 Fragments arranged by size: 3678 1871 1339 411 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2212 3526 5327 6537 2212 Size: 1314 1801 1210 3110 Fragments arranged by size: 3110 1801 1314 1210 BclI T'GATC_A Cuts at: 1548 3390 4626 1548 Size: 1842 1236 4357 Fragments arranged by size: 4357 1842 1236 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3947 6046 136 Size: 107 122 71 631 605 2275 2099 1525 Fragments arranged by size: 2275 2099 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7430 7430 Size: 7435 BlpI GC'TnA_GC Cuts at: 2398 4852 2398 Size: 2454 4981 Bme1580I G_kGCm'C Cuts at: 1790 2635 2690 3982 4249 4416 5496 6652 1790 Size: 845 55 1292 267 167 1080 1156 2573 Fragments arranged by size: 2573 1292 1156 1080 845 267 167 55 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7435 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4114 5166 6124 Size: 416 53 83 399 24 2697 1052 958 Cuts at: 6124 442 Size: 1753 Fragments arranged by size: 2697 1753 1052 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4047 1056 Size: 2991 4444 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2709 2819 3158 4043 6095 1239 Size: 1470 110 339 885 2052 2579 Fragments arranged by size: 2579 2052 1470 885 339 110 Bpu10I CC'TnA_GC Cuts at: 2139 2156 2436 2936 3357 2139 Size: 17 280 500 421 6217 Fragments arranged by size: 6217 500 421 280 17 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2516 4362 5560 6425 6723 2516 Size: 1846 1198 865 298 3228 Fragments arranged by size: 3228 1846 1198 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4103 6098 882 Size: 617 2604 1995 2219 Fragments arranged by size: 2604 2219 1995 617 BsaAI yAC'GTr Cuts at: 493 1204 4096 5226 493 Size: 711 2892 1130 2702 Fragments arranged by size: 2892 2702 1130 711 BsaBI GATnn'nnATC Cuts at: 7429 7429 Size: 7435 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 4440 5242 5624 Size: 53 83 186 936 476 2431 802 382 Cuts at: 5624 6259 275 Size: 635 1451 Fragments arranged by size: 2431 1451 936 802 635 476 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5868 6266 6540 6687 7098 5868 Size: 398 274 147 411 6205 Fragments arranged by size: 6205 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3869 3899 4598 4628 1178 Size: 30 165 30 2466 30 699 30 3985 Fragments arranged by size: 3985 2466 699 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2023 2124 2630 2855 3705 3959 4004 4019 Size: 837 101 506 225 850 254 45 15 Cuts at: 4019 4040 1186 Size: 21 4581 Fragments arranged by size: 4581 850 837 506 254 225 101 45 21 15 BseYI C'CCAG_C Cuts at: 3283 4561 6638 7134 3283 Size: 1278 2077 496 3584 Fragments arranged by size: 3584 2077 1278 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2081 3737 3821 4070 4118 1163 Size: 573 345 1656 84 249 48 4480 Fragments arranged by size: 4480 1656 573 345 249 84 48 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4885 5646 6674 1540 Size: 275 186 2884 761 1028 2301 Fragments arranged by size: 2884 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2865 3879 5496 5581 6652 2865 Size: 1014 1617 85 1071 3648 Fragments arranged by size: 3648 1617 1071 1014 85 BsmI GAATG_Cn' Cuts at: 3064 3686 4648 4946 5039 3064 Size: 622 962 298 93 5460 Fragments arranged by size: 5460 962 622 298 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3626 3626 Size: 7435 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2235 2635 2690 2865 3697 3732 3879 Size: 399 445 400 55 175 832 35 147 Cuts at: 3879 3982 4184 4249 4416 4754 5496 5581 6652 Size: 103 202 65 167 338 742 85 1071 Cuts at: 6652 1391 Size: 2174 Fragments arranged by size: 2174 1071 832 742 445 400 399 338 202 175 167 147 103 85 65 55 35 BspEI T'CCGG_A Cuts at: 7098 7098 Size: 7435 BspHI T'CATG_A Cuts at: 5324 7054 5324 Size: 1730 5705 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3040 3566 4092 4357 4790 844 Size: 2196 526 526 265 433 3489 Fragments arranged by size: 3489 2196 526 526 433 265 BsrBI CCG'CTC Cuts at: 1394 1823 2025 2143 5322 1394 Size: 429 202 118 3179 3507 Fragments arranged by size: 3507 3179 429 202 118 BsrDI GCAATG_nn' Cuts at: 65 1248 2370 3351 4155 4156 4212 5930 6104 Size: 1183 1122 981 804 1 56 1718 174 Cuts at: 6104 65 Size: 1396 Fragments arranged by size: 1718 1396 1183 1122 981 804 174 56 1 BsrFI r'CCGG_y Cuts at: 1700 1810 2445 3399 3815 4112 4508 6079 6266 Size: 110 635 954 416 297 396 1571 187 Cuts at: 6266 1700 Size: 2869 Fragments arranged by size: 2869 1571 954 635 416 396 297 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7435 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7435 BssSI C'ACGA_G Cuts at: 2121 5495 6507 2121 Size: 3374 1012 3049 Fragments arranged by size: 3374 3049 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2685 3556 3681 794 Size: 1891 871 125 4548 Fragments arranged by size: 4548 1891 871 125 BstBI TT'CG_AA Cuts at: 2263 3223 6239 2263 Size: 960 3016 3459 Fragments arranged by size: 3459 3016 960 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7435 BstXI CCAn_nnnn'nTGG Cuts at: 3766 4333 3766 Size: 567 6868 BstYI r'GATC_y Cuts at: 1972 2527 4765 5132 5517 5534 6986 7072 7084 Size: 555 2238 367 385 17 1452 86 12 Cuts at: 7084 7430 1972 Size: 346 1977 Fragments arranged by size: 2238 1977 1452 555 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2538 2538 Size: 7435 BtgI C'CryG_G Cuts at: 513 1065 2004 2046 3999 4383 4443 513 Size: 552 939 42 1953 384 60 3505 Fragments arranged by size: 3505 1953 939 552 384 60 42 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4566 487 Size: 683 56 3340 3356 Fragments arranged by size: 3356 3340 683 56 BtsI GCAGTG_nn' Cuts at: 2626 2722 4951 5745 5765 2626 Size: 96 2229 794 20 4296 Fragments arranged by size: 4296 2229 794 96 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7435 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7400 DraI TTT'AAA Cuts at: 4739 4739 Size: 7435 DraIII CAC_nnn'GTG Cuts at: 2563 2891 4479 2563 Size: 328 1588 5519 Fragments arranged by size: 5519 1588 328 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6442 809 Size: 507 5126 1802 Fragments arranged by size: 5126 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4882 5771 7400 Size: 54 1425 50 275 18 3052 889 1629 Cuts at: 7400 8 Size: 43 Fragments arranged by size: 3052 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4882 1537 Size: 275 3070 4090 Fragments arranged by size: 4090 3070 275 EarI CTCTTCn'nnn_ Cuts at: 1353 5365 1353 Size: 4012 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6022 6396 6542 387 Size: 1064 138 4433 374 146 1280 Fragments arranged by size: 4433 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2863 2863 Size: 7435 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2709 2759 2819 2891 3158 3551 3592 Size: 393 1077 50 60 72 267 393 41 Cuts at: 3592 3606 3718 4043 4339 5498 6095 6882 7150 Size: 14 112 325 296 1159 597 787 268 Cuts at: 7150 1239 Size: 1524 Fragments arranged by size: 1524 1159 1077 787 597 393 393 325 296 268 267 112 72 60 50 41 14 EcoNI CCTnn'n_nnAGG Cuts at: 2870 2870 Size: 7435 EcoO109I rG'GnC_Cy Cuts at: 2042 2631 2632 4315 2042 Size: 589 1 1683 5162 Fragments arranged by size: 5162 1683 589 1 EcoRI G'AATT_C Cuts at: 4744 5122 4744 Size: 378 7057 EcoRV GAT'ATC Cuts at: 4465 4465 Size: 7435 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3767 3913 3767 Size: 146 7289 FspI TGC'GCA Cuts at: 4148 4601 5941 4148 Size: 453 1340 5642 Fragments arranged by size: 5642 1340 453 HaeII r_GCGC'y Cuts at: 1678 4668 4687 6582 1678 Size: 2990 19 1895 2531 Fragments arranged by size: 2990 2531 1895 19 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2034 2066 3404 3436 6076 Size: 32 135 32 462 32 1338 32 2640 Cuts at: 6076 6108 6150 6182 1373 Size: 32 42 32 2626 Fragments arranged by size: 2640 2626 1338 462 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4779 5025 669 Size: 988 281 2841 246 3079 Fragments arranged by size: 3079 2841 988 281 246 HindIII A'AGCT_T Cuts at: 748 3230 748 Size: 2482 4953 HpaI GTT'AAC Cuts at: 5025 5025 Size: 7435 KpnI G_GTAC'C Cuts at: 2832 4243 4341 4760 2832 Size: 1411 98 419 5507 Fragments arranged by size: 5507 1411 419 98 MfeI C'AATT_G Cuts at: 5034 5034 Size: 7435 MluI A'CGCG_T Cuts at: 7311 7311 Size: 7435 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3148 4104 6549 6733 1305 Size: 1843 956 2445 184 2007 Fragments arranged by size: 2445 2007 1843 956 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5613 Fragments arranged by size: 5613 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2078 2844 3074 3432 4364 4409 4503 Size: 648 912 766 230 358 932 45 94 Cuts at: 4503 5393 5752 5911 518 Size: 890 359 159 2042 Fragments arranged by size: 2042 932 912 890 766 648 359 358 230 159 94 45 MspA1I CmG'CkG Cuts at: 1606 2048 2065 3571 4851 5528 6676 6921 1606 Size: 442 17 1506 1280 677 1148 245 2120 Fragments arranged by size: 2120 1506 1280 1148 677 442 245 17 NaeI GCC'GGC Cuts at: 3817 4510 3817 Size: 693 6742 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5944 Fragments arranged by size: 5944 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7435 NgoMIV G'CCGG_C Cuts at: 3815 4508 3815 Size: 693 6742 NheI G'CTAG_C Cuts at: 1052 4043 1052 Size: 2991 4444 NotI GC'GGCC_GC Cuts at: 4882 4882 Size: 7435 NruI TCG'CGA Cuts at: 2767 2767 Size: 7435 NspI r_CATG'y Cuts at: 1132 1229 2609 3202 4147 4286 4793 6338 1132 Size: 97 1380 593 945 139 507 1545 2229 Fragments arranged by size: 2229 1545 1380 945 593 507 139 97 PasI CC'CwG_GG Cuts at: 1348 2235 2317 1348 Size: 887 82 6466 Fragments arranged by size: 6466 887 82 PciI A'CATG_T Cuts at: 3198 4282 6334 3198 Size: 1084 2052 4299 Fragments arranged by size: 4299 2052 1084 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7435 PfoI T'CCnGG_A Cuts at: 2523 2523 Size: 7435 PmeI GTTT'AAAC Cuts at: 4739 4739 Size: 7435 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4598 4630 5504 5536 7017 7049 4598 Size: 32 874 32 1481 32 4984 Fragments arranged by size: 4984 1481 874 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2042 4315 2042 Size: 2273 5162 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7435 PsiI TTA'TAA Cuts at: 5005 5005 Size: 7435 PspOMI G'GGCC_C Cuts at: 2631 3978 2631 Size: 1347 6088 PstI C_TGCA'G Cuts at: 830 2743 3451 3777 4533 4787 830 Size: 1913 708 326 756 254 3478 Fragments arranged by size: 3478 1913 756 708 326 254 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7435 SacI G_AGCT'C Cuts at: 2865 2865 Size: 7435 SacII CC_GC'GG Cuts at: 2049 2049 Size: 7435 SalI G'TCGA_C Cuts at: 1936 4777 1936 Size: 2841 4594 SanDI GG'GwC_CC Cuts at: 2042 2042 Size: 7435 SbfI CC_TGCA'GG Cuts at: 4787 4787 Size: 7435 ScaI AGT'ACT Cuts at: 5683 7395 5683 Size: 1712 5723 SexAI A'CCwGG_T Cuts at: 2655 2655 Size: 7435 SfcI C'TryA_G Cuts at: 826 1045 2739 3447 3773 4173 4529 4726 4783 Size: 219 1694 708 326 400 356 197 57 Cuts at: 4783 5918 6599 6790 826 Size: 1135 681 191 1471 Fragments arranged by size: 1694 1471 1135 708 681 400 356 326 219 197 191 57 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7435 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7435 SmaI CCC'GGG Cuts at: 1872 2921 3212 4762 1872 Size: 1049 291 1550 4545 Fragments arranged by size: 4545 1550 1049 291 SmlI C'TyrA_G Cuts at: 820 1017 1943 2250 2531 4377 5539 6440 6702 Size: 197 926 307 281 1846 1162 901 262 Cuts at: 6702 820 Size: 1553 Fragments arranged by size: 1846 1553 1162 926 901 307 281 262 197 SnaBI TAC'GTA Cuts at: 493 5226 493 Size: 4733 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7435 SphI G_CATG'C Cuts at: 1132 4793 1132 Size: 3661 3774 SspI AAT'ATT Cuts at: 5 52 5359 5 Size: 47 5307 2081 Fragments arranged by size: 5307 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7435 StyI C'CwwG_G Cuts at: 513 1065 2004 2344 2552 3039 3759 3888 4545 Size: 552 939 340 208 487 720 129 657 Cuts at: 4545 4874 513 Size: 329 3074 Fragments arranged by size: 3074 939 720 657 552 487 340 329 208 129 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3167 4082 4133 5462 5479 5632 5817 Size: 1255 1587 915 51 1329 17 153 185 Cuts at: 5817 6262 325 Size: 445 1498 Fragments arranged by size: 1587 1498 1329 1255 915 445 185 153 51 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5681 7393 96 Size: 274 80 33 51 1443 3704 1712 138 Fragments arranged by size: 3704 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2482 2505 2514 2537 3869 3901 7395 7427 2482 Size: 23 9 23 1332 32 3494 32 2490 Fragments arranged by size: 3494 2490 1332 32 32 23 23 9 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7435 XbaI T'CTAG_A Cuts at: 4771 4771 Size: 7435 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3007 4552 3007 Size: 1545 5890 XhoI C'TCGA_G Cuts at: 1943 2250 1943 Size: 307 7128 XmaI C'CCGG_G Cuts at: 1870 2919 3210 4760 1870 Size: 1049 291 1550 4545 Fragments arranged by size: 4545 1550 1049 291 XmnI GAAnn'nnTTC Cuts at: 3219 4613 5564 3219 Size: 1394 951 5090 Fragments arranged by size: 5090 1394 951 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5243 276 Size: 53 83 186 936 3709 2468 Fragments arranged by size: 3709 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI Bsu36I FseI FspAI KasI NarI NsiI PacI PmlI PspXI PsrI PvuII RsrII SapI SfiI SfoI SrfI SwaI