(Circular) (Six-base) MAPSORT of: pFN21AB4450.seq Check: 8110 from: 1 to: 5845 pFN21AB4450 5845 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2897 2982 2897 Size: 85 5760 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3655 278 Size: 53 83 186 936 2119 2468 Fragments arranged by size: 2468 2119 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3188 1937 Size: 1251 4594 Acc65I G'GTAC_C Cuts at: 3166 3166 Size: 5845 AclI AA'CG_TT Cuts at: 1563 3972 4345 1563 Size: 2409 373 3063 Fragments arranged by size: 3063 2409 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3908 5292 5560 1632 Size: 2276 1384 268 1917 Fragments arranged by size: 2276 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5648 AflIII A'CryG_T Cuts at: 1203 2109 2268 2340 4744 5721 1203 Size: 906 159 72 2404 977 1327 Fragments arranged by size: 2404 1327 977 906 159 72 AgeI A'CCGG_T Cuts at: 4676 4676 Size: 5845 AhdI GACnn_n'nnGTC Cuts at: 2050 4574 2050 Size: 2524 3321 AleI CACnn'nnGTG Cuts at: 1166 2537 2624 1166 Size: 1371 87 4387 Fragments arranged by size: 4387 1371 87 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2849 2987 1184 Size: 1665 138 4042 Fragments arranged by size: 4042 1665 138 AlwNI CAG_nnn'CTG Cuts at: 3588 5160 3588 Size: 1572 4273 ApaI G_GGCC'C Cuts at: 2092 3066 2092 Size: 974 4871 ApaLI G'TGCA_C Cuts at: 3902 5058 3902 Size: 1156 4689 ApoI r'AATT_y Cuts at: 1493 3154 3380 3532 5641 1493 Size: 1661 226 152 2109 1697 Fragments arranged by size: 2109 1697 1661 226 152 AseI AT'TA_AT Cuts at: 160 1032 4399 160 Size: 872 3367 1606 Fragments arranged by size: 3367 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5845 AvaI C'yCGr_G Cuts at: 1870 1943 2083 3170 1870 Size: 73 140 1087 4545 Fragments arranged by size: 4545 1087 140 73 BamHI G'GATC_C Cuts at: 3175 3542 3175 Size: 367 5478 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2129 2638 2733 3002 3166 Size: 325 217 627 342 509 95 269 164 Cuts at: 3166 4621 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 509 342 325 269 217 164 95 BanII G_rGCy'C Cuts at: 1391 2092 2582 3066 3164 1391 Size: 701 490 484 98 4072 Fragments arranged by size: 4072 701 490 484 98 BbeI G_GCGC'C Cuts at: 2737 2737 Size: 5845 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2110 2347 2813 928 Size: 971 211 237 466 3960 Fragments arranged by size: 3960 971 466 237 211 BbvCI CC'TCA_GC Cuts at: 2372 2423 2372 Size: 51 5794 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4036 4070 1903 Size: 34 2099 34 3678 Fragments arranged by size: 3678 2099 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3737 4947 3737 Size: 1210 4635 BclI T'GATC_A Cuts at: 1548 1548 Size: 5845 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2367 2483 4456 Size: 107 122 71 631 605 695 116 1973 Cuts at: 4456 136 Size: 1525 Fragments arranged by size: 1973 1525 695 631 605 122 116 107 71 BglII A'GATC_T Cuts at: 5840 5840 Size: 5845 BlpI GC'TnA_GC Cuts at: 2191 2315 2744 3262 2191 Size: 124 429 518 4774 Fragments arranged by size: 4774 518 429 124 Bme1580I G_kGCm'C Cuts at: 1790 2092 2132 2643 3054 3066 3906 5062 1790 Size: 302 40 511 411 12 840 1156 2573 Fragments arranged by size: 2573 1156 840 511 411 302 40 12 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5845 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3576 4534 442 Size: 416 53 83 399 24 2159 958 1753 Fragments arranged by size: 2159 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2214 1056 Size: 1158 4687 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4505 1239 Size: 3266 2579 Bpu10I CC'TnA_GC Cuts at: 2372 2423 2372 Size: 51 5794 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3970 4835 5133 3970 Size: 865 298 4682 Fragments arranged by size: 4682 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2617 3122 4508 882 Size: 617 1118 505 1386 2219 Fragments arranged by size: 2219 1386 1118 617 505 BsaAI yAC'GTr Cuts at: 493 1204 2271 3636 493 Size: 711 1067 1365 2702 Fragments arranged by size: 2702 1365 1067 711 BsaBI GATnn'nnATC Cuts at: 5839 5839 Size: 5845 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2734 3652 4034 4669 Size: 53 83 186 936 1201 918 382 635 Cuts at: 4669 275 Size: 1451 Fragments arranged by size: 1451 1201 936 918 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4278 4676 4950 5097 5508 4278 Size: 398 274 147 411 4615 Fragments arranged by size: 4615 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5620 Fragments arranged by size: 5620 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5845 BseYI C'CCAG_C Cuts at: 2821 5048 5544 2821 Size: 2227 496 3122 Fragments arranged by size: 3122 2227 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2561 2819 2869 2947 1163 Size: 573 825 258 50 78 4061 Fragments arranged by size: 4061 825 573 258 78 50 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2383 3295 4056 5084 1540 Size: 275 186 382 912 761 1028 2301 Fragments arranged by size: 2301 1028 912 761 382 275 186 BsiHKAI G_wGCw'C Cuts at: 2154 2582 3906 3991 5062 2154 Size: 428 1324 85 1071 2937 Fragments arranged by size: 2937 1324 1071 428 85 BsmI GAATG_Cn' Cuts at: 3356 3449 3356 Size: 93 5752 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2092 2132 2154 2582 2643 3054 3066 Size: 399 302 40 22 428 61 411 12 Cuts at: 3066 3164 3906 3991 5062 1391 Size: 98 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 428 411 399 302 98 85 61 40 22 12 BspEI T'CCGG_A Cuts at: 5508 5508 Size: 5845 BspHI T'CATG_A Cuts at: 2865 3734 5464 2865 Size: 869 1730 3246 Fragments arranged by size: 3246 1730 869 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2897 2982 3200 844 Size: 2053 85 218 3489 Fragments arranged by size: 3489 2053 218 85 BsrBI CCG'CTC Cuts at: 1394 1823 3732 1394 Size: 429 1909 3507 Fragments arranged by size: 3507 1909 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2205 4340 4514 65 Size: 1183 957 2135 174 1396 Fragments arranged by size: 2135 1396 1183 957 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2366 2736 4489 4676 1700 Size: 110 556 370 1753 187 2869 Fragments arranged by size: 2869 1753 556 370 187 110 BsrGI T'GTAC_A Cuts at: 96 2814 96 Size: 2718 3127 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5845 BssSI C'ACGA_G Cuts at: 3101 3905 4917 3101 Size: 804 1012 4029 Fragments arranged by size: 4029 1012 804 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5845 BstBI TT'CG_AA Cuts at: 4649 4649 Size: 5845 BstEII G'GTnAC_C Cuts at: 1184 2767 1184 Size: 1583 4262 BstXI CCAn_nnnn'nTGG Cuts at: 2626 2626 Size: 5845 BstYI r'GATC_y Cuts at: 1972 3175 3542 3927 3944 5396 5482 5494 5840 Size: 1203 367 385 17 1452 86 12 346 Cuts at: 5840 1972 Size: 1977 Fragments arranged by size: 1977 1452 1203 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2133 2445 2451 2562 2625 513 Size: 552 939 129 312 6 111 63 3733 Fragments arranged by size: 3733 939 552 312 129 111 63 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2659 2671 487 Size: 683 56 1433 12 3661 Fragments arranged by size: 3661 1433 683 56 12 BtsI GCAGTG_nn' Cuts at: 2549 3025 3361 4155 4175 2549 Size: 476 336 794 20 4219 Fragments arranged by size: 4219 794 476 336 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5845 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5810 DraI TTT'AAA Cuts at: 3149 3149 Size: 5845 DraIII CAC_nnn'GTG Cuts at: 2803 2803 Size: 5845 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4852 809 Size: 507 3536 1802 Fragments arranged by size: 3536 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2391 2409 2448 Size: 54 1425 50 275 18 561 18 39 Cuts at: 2448 2472 2484 2568 2692 2983 3292 4181 5810 Size: 24 12 84 124 291 309 889 1629 Cuts at: 5810 8 Size: 43 Fragments arranged by size: 1629 1425 889 561 309 291 275 124 84 54 50 43 39 24 18 18 12 EagI C'GGCC_G Cuts at: 1537 1812 3292 1537 Size: 275 1480 4090 Fragments arranged by size: 4090 1480 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2177 2864 3049 3775 1353 Size: 824 687 185 726 3423 Fragments arranged by size: 3423 824 726 687 185 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2942 4432 4806 4952 387 Size: 1064 138 1353 1490 374 146 1280 Fragments arranged by size: 1490 1353 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2580 2580 Size: 5845 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3908 4505 5292 5560 1239 Size: 393 2276 597 787 268 1524 Fragments arranged by size: 2276 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2088 2089 2298 2719 3062 2088 Size: 1 209 421 343 4871 Fragments arranged by size: 4871 421 343 209 1 EcoRI G'AATT_C Cuts at: 3154 3532 3154 Size: 378 5467 EcoRV GAT'ATC Cuts at: 2200 2200 Size: 5845 FspI TGC'GCA Cuts at: 2998 4351 2998 Size: 1353 4492 HaeII r_GCGC'y Cuts at: 1678 2191 2737 4992 1678 Size: 513 546 2255 2531 Fragments arranged by size: 2531 2255 546 513 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2036 2063 2068 2095 4486 Size: 32 135 32 464 27 5 27 2391 Cuts at: 4486 4518 4560 4592 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2391 464 135 42 32 32 32 32 27 27 5 HincII GTy'rAC Cuts at: 669 1657 1938 2338 2905 3142 3189 3435 669 Size: 988 281 400 567 237 47 246 3079 Fragments arranged by size: 3079 988 567 400 281 246 237 47 HindIII A'AGCT_T Cuts at: 748 748 Size: 5845 HpaI GTT'AAC Cuts at: 3435 3435 Size: 5845 KasI G'GCGC_C Cuts at: 2733 2733 Size: 5845 KpnI G_GTAC'C Cuts at: 3170 3170 Size: 5845 MfeI C'AATT_G Cuts at: 3444 3444 Size: 5845 MluI A'CGCG_T Cuts at: 5721 5721 Size: 5845 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4959 5143 1305 Size: 3654 184 2007 Fragments arranged by size: 3654 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2393 2411 2450 2486 2570 Size: 54 1425 343 561 18 39 36 84 Cuts at: 2570 2694 2985 10 Size: 124 291 2870 Fragments arranged by size: 2870 1425 561 343 291 124 84 54 39 36 18 MslI CAynn'nnrTG Cuts at: 518 1166 2108 2537 2624 2651 2753 2756 2969 Size: 648 942 429 87 27 102 3 213 Cuts at: 2969 3803 4162 4321 518 Size: 834 359 159 2042 Fragments arranged by size: 2042 942 834 648 429 359 213 159 102 87 27 3 MspA1I CmG'CkG Cuts at: 1606 2195 2439 2910 3261 3938 5086 5331 1606 Size: 589 244 471 351 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 589 471 351 245 244 NaeI GCC'GGC Cuts at: 2738 2738 Size: 5845 NarI GG'CG_CC Cuts at: 2734 2734 Size: 5845 NcoI C'CATG_G Cuts at: 513 1065 2004 2562 513 Size: 552 939 558 3796 Fragments arranged by size: 3796 939 558 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5845 NgoMIV G'CCGG_C Cuts at: 2736 2736 Size: 5845 NheI G'CTAG_C Cuts at: 1052 2210 1052 Size: 1158 4687 NotI GC'GGCC_GC Cuts at: 3292 3292 Size: 5845 NspI r_CATG'y Cuts at: 1132 1229 2113 2344 2821 3203 4748 1132 Size: 97 884 231 477 382 1545 2229 Fragments arranged by size: 2229 1545 884 477 382 231 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5845 PciI A'CATG_T Cuts at: 2109 2340 4744 2109 Size: 231 2404 3210 Fragments arranged by size: 3210 2404 231 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5845 PmeI GTTT'AAAC Cuts at: 3149 3149 Size: 5845 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3914 3946 5427 5459 3914 Size: 32 1481 32 4300 Fragments arranged by size: 4300 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2298 2298 Size: 5845 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5845 PsiI TTA'TAA Cuts at: 3415 3415 Size: 5845 PspOMI G'GGCC_C Cuts at: 2088 3062 2088 Size: 974 4871 PstI C_TGCA'G Cuts at: 830 3197 830 Size: 2367 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5845 PvuII CAG'CTG Cuts at: 2195 2910 2195 Size: 715 5130 SacI G_AGCT'C Cuts at: 2582 2582 Size: 5845 SalI G'TCGA_C Cuts at: 1936 3187 1936 Size: 1251 4594 SbfI CC_TGCA'GG Cuts at: 3197 3197 Size: 5845 ScaI AGT'ACT Cuts at: 4093 5805 4093 Size: 1712 4133 SexAI A'CCwGG_T Cuts at: 2799 2799 Size: 5845 SfcI C'TryA_G Cuts at: 826 1045 3118 3193 4328 5009 5200 826 Size: 219 2073 75 1135 681 191 1471 Fragments arranged by size: 2073 1471 1135 681 219 191 75 SfoI GGC'GCC Cuts at: 2735 2735 Size: 5845 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5845 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5845 SmaI CCC'GGG Cuts at: 1872 3172 1872 Size: 1300 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3949 4850 5112 820 Size: 197 926 2006 901 262 1553 Fragments arranged by size: 2006 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3636 493 Size: 3143 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5845 SphI G_CATG'C Cuts at: 1132 3203 1132 Size: 2071 3774 SspI AAT'ATT Cuts at: 5 52 3769 5 Size: 47 3717 2081 Fragments arranged by size: 3717 2081 47 StuI AGG'CCT Cuts at: 1517 2960 1517 Size: 1443 4402 StyI C'CwwG_G Cuts at: 513 1065 2004 2562 2605 3284 513 Size: 552 939 558 43 679 3074 Fragments arranged by size: 3074 939 679 558 552 43 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2455 3872 3889 4042 4227 4672 325 Size: 1255 875 1417 17 153 185 445 1498 Fragments arranged by size: 1498 1417 1255 875 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2064 2814 4091 Size: 274 80 33 51 1443 87 750 1277 Cuts at: 4091 5803 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1277 750 274 138 87 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3092 3124 5805 5837 3092 Size: 32 2681 32 3100 Fragments arranged by size: 3100 2681 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5845 XbaI T'CTAG_A Cuts at: 3181 3181 Size: 5845 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2110 2494 2518 2687 3017 2110 Size: 384 24 169 330 4938 Fragments arranged by size: 4938 384 330 169 24 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5845 XmaI C'CCGG_G Cuts at: 1870 3170 1870 Size: 1300 4545 XmnI GAAnn'nnTTC Cuts at: 2873 3974 2873 Size: 1101 4744 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3653 276 Size: 53 83 186 936 2119 2468 Fragments arranged by size: 2468 2119 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoNI FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SapI SfiI SrfI SwaI