Restriction map for
pFN21AB4394
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# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Aor51HI AscI AseI AsnI
Asp700I AspEI AspI BamHI BfrI
BglII BlpI Bpu1102I BsaBI BsaWI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrGI Bst1107I BstBI BstEII BstPI
BstZ17I CelII ClaI CpoI DraI
Eam1105I Eco47III EcoO65I EcoRV EcoT22I
FseI HindIII HpaI KspI MamI
MfeI MroI MunI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI ScaI
SexAI SfuI SgrAI SnaBI SpeI
SspBI SspI SwaI TfiI Tth111I
XbaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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Acc65I 911 GGTACC G^GTACC
AccI 2161 GTCTAC GT^[AC][GT]AC
AcsI 1951 GAATTC [GA]^AATT[CT]
AflIII 1518 ACGCGT A^C[GA][CT]GT
AocI 1632 CCTCAGG CC^T[ACGT]AGG
ApoI 1951 GAATTC [GA]^AATT[CT]
Asp718I 911 GGTACC G^GTACC
AvrII 288 CCTAGG C^CTAGG
BciVI 1192 GTATCC GTATCC(6/5)
BclI 1529 TGATCA T^GATCA
BlnI 288 CCTAGG C^CTAGG
BspMI 2381 ACCTGC ACCTGC(4/8)
BsrDI 174 GCAATG GCAATG(2/0)
BstYI 695 AGATCC [GA]^GATC[CT]
Bsu36I 1632 CCTCAGG CC^T[ACGT]AGG
DrdI 1249 GACCTGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1281 GAAGAG CTCTTC(1/4)
Eco81I 1632 CCTCAGG CC^T[ACGT]AGG
EcoRI 1951 GAATTC G^AATTC
FbaI 1529 TGATCA T^GATCA
HincII 1246 GTTGAC GT[CT]^[GA]AC
HindII 1246 GTTGAC GT[CT]^[GA]AC
KpnI 911 GGTACC GGTAC^C
Ksp632I 1281 GAAGAG CTCTTC(1/4)
MflI 695 AGATCC [GA]^GATC[CT]
MluI 1518 ACGCGT A^CGCGT
MseI 1937 TTAA T^TAA
NcoI 2084 CCATGG C^CATGG
PaeR7I 2125 CTCGAG C^TCGAG
PflMI 2458 CCACTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SfiI 796 GGCCAGCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 254 GCATGC GCATG^C
StuI 2409 AGGCCT AGG^CCT
Tru9I 1937 TTAA T^TAA
Tsp509I 1952 AATT ^AATT
Van91I 2458 CCACTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1485 CCACGTGGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2125 CTCGAG C^TCGAG
XhoII 695 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Alw44I 384 GTGCAC G^TGCAC
2285 GTGCAC
AlwI 696 GATCC GGATC(4/5)
834 GATCC
ApaI 1922 GGGCCC GGGCC^C
2139 GGGCCC
ApaLI 384 GTGCAC G^TGCAC
2285 GTGCAC
BbrPI 865 CACGTG CAC^GTG
1486 CACGTG
BcgI 200 CGAAGCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1647 CGAGAACCTTGC
BsaAI 865 CACGTG [CT]AC^GT[GA]
1486 CACGTG
BsiEI 404 CGGCCG CG[GA][CT]^CG
788 CGGCCG
BsmFI 1232 GGGAC GGGAC(10/14)
1815 GTCCC
BsrFI 356 ACCGGC [GA]^CCGG[CT]
2300 ACCGGC
BssSI 481 CACGAG CACGAG(-5/-1)
639 CTCGTG
BstXI 938 CCAGTCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1416 CCACCTGGATGG
Cfr10I 356 ACCGGC [GA]^CCGG[CT]
2300 ACCGGC
DraIII 222 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
2257 CACAACGTG
DsaI 2084 CCATGG C^C[GA][CT]GG
2469 CCGTGG
EagI 404 CGGCCG C^GGCCG
788 CGGCCG
EclXI 404 CGGCCG C^GGCCG
788 CGGCCG
Eco52I 404 CGGCCG C^GGCCG
788 CGGCCG
PmaCI 865 CACGTG CAC^GTG
1486 CACGTG
PmlI 865 CACGTG CAC^GTG
1486 CACGTG
SbfI 1146 CCTGCAGG CCTGCA^GG
2382 CCTGCAGG
Sse8387I 1146 CCTGCAGG CCTGCA^GG
2382 CCTGCAGG
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# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AspHI AvaI AvaII AviII
BalI BanI BanII BbeI BbsI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsoBI Bsp1286I BsrI
BssHII BssKI BstAPI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
FspI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SmlI StyI TaqI TseI
Tsp45I TspRI TthHB8I XmaCI XmaI