(Circular) (Six-base) MAPSORT of: pFN21AB4394.seq Check: 6913 from: 1 to: 7207 pFN21AB4394 7207 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5017 278 Size: 53 83 186 936 3481 2468 Fragments arranged by size: 3481 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4167 4550 1937 Size: 2230 383 4594 Fragments arranged by size: 4594 2230 383 Acc65I G'GTAC_C Cuts at: 2916 4528 2916 Size: 1612 5595 AclI AA'CG_TT Cuts at: 1563 5334 5707 1563 Size: 3771 373 3063 Fragments arranged by size: 3771 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2115 3781 3807 4159 4340 5270 6654 6922 Size: 483 1666 26 352 181 930 1384 268 Cuts at: 6922 1632 Size: 1917 Fragments arranged by size: 1917 1666 1384 930 483 352 268 181 26 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7010 AflIII A'CryG_T Cuts at: 1203 3523 6106 7083 1203 Size: 2320 2583 977 1327 Fragments arranged by size: 2583 2320 1327 977 AgeI A'CCGG_T Cuts at: 6038 6038 Size: 7207 AhdI GACnn_n'nnGTC Cuts at: 5936 5936 Size: 7207 AleI CACnn'nnGTG Cuts at: 1166 2470 4299 1166 Size: 1304 1829 4074 Fragments arranged by size: 4074 1829 1304 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2775 4209 1184 Size: 1591 1434 4182 Fragments arranged by size: 4182 1591 1434 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2141 2173 3749 3781 2141 Size: 32 1576 32 5567 Fragments arranged by size: 5567 1576 32 32 AlwNI CAG_nnn'CTG Cuts at: 2167 2743 3103 3221 4210 4396 4950 6522 2167 Size: 576 360 118 989 186 554 1572 2852 Fragments arranged by size: 2852 1572 989 576 554 360 186 118 ApaI G_GGCC'C Cuts at: 3931 4148 3931 Size: 217 6990 ApaLI G'TGCA_C Cuts at: 2389 4290 5264 6420 2389 Size: 1901 974 1156 3176 Fragments arranged by size: 3176 1901 1156 974 ApoI r'AATT_y Cuts at: 1493 3956 4516 4742 4894 7003 1493 Size: 2463 560 226 152 2109 1697 Fragments arranged by size: 2463 2109 1697 560 226 152 AseI AT'TA_AT Cuts at: 160 1032 5761 160 Size: 872 4729 1606 Fragments arranged by size: 4729 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7207 AvaI C'yCGr_G Cuts at: 1870 1943 2081 3576 3973 4016 4130 4141 4233 Size: 73 138 1495 397 43 114 11 92 Cuts at: 4233 4532 1870 Size: 299 4545 Fragments arranged by size: 4545 1495 397 299 138 114 92 73 43 11 AvrII C'CTAG_G Cuts at: 2293 2293 Size: 7207 BamHI G'GATC_C Cuts at: 4537 4904 4537 Size: 367 6840 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2281 2346 2543 2916 3503 Size: 325 217 627 494 65 197 373 587 Cuts at: 3503 4400 4528 5983 618 Size: 897 128 1455 1842 Fragments arranged by size: 1842 1455 897 627 587 494 373 325 217 197 128 65 BanII G_rGCy'C Cuts at: 1391 2067 2140 2679 2881 3096 3374 3452 3863 Size: 676 73 539 202 215 278 78 411 Cuts at: 3863 3931 4148 4526 1391 Size: 68 217 378 4072 Fragments arranged by size: 4072 676 539 411 378 278 217 215 202 78 73 68 BbeI G_GCGC'C Cuts at: 2285 2350 3507 2285 Size: 65 1157 5985 Fragments arranged by size: 5985 1157 65 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3463 3555 3684 928 Size: 971 1564 92 129 4451 Fragments arranged by size: 4451 1564 971 129 92 BbvCI CC'TCA_GC Cuts at: 3161 3848 4239 3161 Size: 687 391 6129 Fragments arranged by size: 6129 687 391 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2194 2228 3641 3675 5398 5432 1903 Size: 34 257 34 1413 34 1723 34 3678 Fragments arranged by size: 3678 1723 1413 257 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3208 5099 6309 3208 Size: 1891 1210 4106 Fragments arranged by size: 4106 1891 1210 BclI T'GATC_A Cuts at: 1548 3534 1548 Size: 1986 5221 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2808 3512 4103 Size: 107 122 71 631 605 1136 704 591 Cuts at: 4103 5818 136 Size: 1715 1525 Fragments arranged by size: 1715 1525 1136 704 631 605 591 122 107 71 BglII A'GATC_T Cuts at: 7202 7202 Size: 7207 BlpI GC'TnA_GC Cuts at: 4624 4624 Size: 7207 Bme1580I G_kGCm'C Cuts at: 1790 2204 2270 2393 3314 3931 4017 4148 4294 Size: 414 66 123 921 617 86 131 146 Cuts at: 4294 4403 5268 6424 1790 Size: 109 865 1156 2573 Fragments arranged by size: 2573 1156 921 865 617 414 146 131 123 109 86 66 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7207 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3301 4938 5896 Size: 416 53 83 399 24 1884 1637 958 Cuts at: 5896 442 Size: 1753 Fragments arranged by size: 1884 1753 1637 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7207 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2107 3988 4510 2107 Size: 1881 522 4804 Fragments arranged by size: 4804 1881 522 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2267 2918 3599 3845 3953 5867 1239 Size: 1028 651 681 246 108 1914 2579 Fragments arranged by size: 2579 1914 1028 681 651 246 108 Bpu10I CC'TnA_GC Cuts at: 2236 2880 3161 3848 4239 2236 Size: 644 281 687 391 5204 Fragments arranged by size: 5204 687 644 391 281 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2465 2663 3689 4022 5332 6197 6495 2465 Size: 198 1026 333 1310 865 298 3177 Fragments arranged by size: 3177 1310 1026 865 333 298 198 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2078 2819 4141 5870 882 Size: 617 579 741 1322 1729 2219 Fragments arranged by size: 2219 1729 1322 741 617 579 BsaAI yAC'GTr Cuts at: 493 1204 2872 3493 4998 493 Size: 711 1668 621 1505 2702 Fragments arranged by size: 2702 1668 1505 711 621 BsaBI GATnn'nnATC Cuts at: 7201 7201 Size: 7207 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2252 2282 2347 2727 Size: 53 83 186 936 719 30 65 380 Cuts at: 2727 3504 5014 5396 6031 275 Size: 777 1510 382 635 1451 Fragments arranged by size: 1510 1451 936 777 719 635 382 380 186 83 65 53 30 BsaWI w'CCGG_w Cuts at: 5640 6038 6312 6459 6870 5640 Size: 398 274 147 411 5977 Fragments arranged by size: 5977 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2059 2089 2141 2147 2171 Size: 30 165 30 656 30 52 6 24 Cuts at: 2171 2177 3344 3374 3557 3587 1178 Size: 6 1167 30 183 30 4798 Fragments arranged by size: 4798 1167 656 183 165 52 30 30 30 30 30 24 6 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2165 2168 2432 2732 2846 2948 3104 3356 Size: 979 3 264 300 114 102 156 252 Cuts at: 3356 4118 1186 Size: 762 4275 Fragments arranged by size: 4275 979 762 300 264 252 156 114 102 3 BseYI C'CCAG_C Cuts at: 2037 2108 2114 2120 2806 2950 3392 3629 4149 Size: 71 6 6 686 144 442 237 520 Cuts at: 4149 4158 4371 4497 6410 6906 2037 Size: 9 213 126 1913 496 2338 Fragments arranged by size: 2338 1913 686 520 496 442 237 213 144 126 71 9 6 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1998 2207 2319 3023 3316 4235 1163 Size: 573 262 209 112 704 293 919 4135 Fragments arranged by size: 4135 919 704 573 293 262 209 112 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2412 2796 4657 5418 6446 1540 Size: 275 186 411 384 1861 761 1028 2301 Fragments arranged by size: 2301 1861 1028 761 411 384 275 186 BsiHKAI G_wGCw'C Cuts at: 2237 2393 2679 3374 3863 4294 5268 5353 6424 Size: 156 286 695 489 431 974 85 1071 Cuts at: 6424 2237 Size: 3020 Fragments arranged by size: 3020 1071 974 695 489 431 286 156 85 BsmI GAATG_Cn' Cuts at: 4718 4811 4718 Size: 93 7114 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2067 2140 2204 2237 2270 2393 2679 Size: 399 277 73 64 33 33 123 286 Cuts at: 2679 2881 3096 3314 3374 3452 3863 3931 4017 Size: 202 215 218 60 78 411 68 86 Cuts at: 4017 4148 4294 4403 4526 5268 5353 6424 1391 Size: 131 146 109 123 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 411 399 286 277 218 215 202 146 131 123 123 109 86 85 78 73 68 64 60 33 33 BspEI T'CCGG_A Cuts at: 6870 6870 Size: 7207 BspHI T'CATG_A Cuts at: 5096 6826 5096 Size: 1730 5477 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4395 4562 844 Size: 3551 167 3489 Fragments arranged by size: 3551 3489 167 BsrBI CCG'CTC Cuts at: 1394 1823 5094 1394 Size: 429 3271 3507 Fragments arranged by size: 3507 3271 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2186 5702 5876 65 Size: 1183 938 3516 174 1396 Fragments arranged by size: 3516 1396 1183 938 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2361 4305 5851 6038 1700 Size: 110 551 1944 1546 187 2869 Fragments arranged by size: 2869 1944 1546 551 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7207 BssHII G'CGCG_C Cuts at: 1925 2649 2814 2994 1925 Size: 724 165 180 6138 Fragments arranged by size: 6138 724 180 165 BssSI C'ACGA_G Cuts at: 2486 2644 5267 6279 2486 Size: 158 2623 1012 3414 Fragments arranged by size: 3414 2623 1012 158 BstAPI GCAn_nnn'nTGC Cuts at: 794 2265 2397 2613 2743 794 Size: 1471 132 216 130 5258 Fragments arranged by size: 5258 1471 216 132 130 BstBI TT'CG_AA Cuts at: 6011 6011 Size: 7207 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7207 BstXI CCAn_nnnn'nTGG Cuts at: 2950 3428 2950 Size: 478 6729 BstYI r'GATC_y Cuts at: 1972 2700 4537 4904 5289 5306 6758 6844 6856 Size: 728 1837 367 385 17 1452 86 12 Cuts at: 6856 7202 1972 Size: 346 1977 Fragments arranged by size: 1977 1837 1452 728 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3638 3638 Size: 7207 BtgI C'CryG_G Cuts at: 513 1065 2004 4089 4474 513 Size: 552 939 2085 385 3246 Fragments arranged by size: 3246 2085 939 552 385 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6468 Fragments arranged by size: 6468 683 56 BtsI GCAGTG_nn' Cuts at: 2270 3604 4193 4723 5517 5537 2270 Size: 1334 589 530 794 20 3940 Fragments arranged by size: 3940 1334 794 589 530 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7207 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4447 4482 529 Size: 35 3883 35 3254 Fragments arranged by size: 3883 3254 35 35 DraI TTT'AAA Cuts at: 4511 4511 Size: 7207 DraIII CAC_nnn'GTG Cuts at: 2232 4267 2232 Size: 2035 5172 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3260 6214 809 Size: 507 1944 2954 1802 Fragments arranged by size: 2954 1944 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2409 2793 2800 Size: 54 1425 50 275 18 579 384 7 Cuts at: 2800 2940 3495 4104 4471 4654 5543 7172 8 Size: 140 555 609 367 183 889 1629 43 Fragments arranged by size: 1629 1425 889 609 579 555 384 367 275 183 140 54 50 43 18 7 EagI C'GGCC_G Cuts at: 1537 1812 2409 2793 4654 1537 Size: 275 597 384 1861 4090 Fragments arranged by size: 4090 1861 597 384 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3281 5137 1353 Size: 1928 1856 3423 Fragments arranged by size: 3423 1928 1856 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2204 5794 6168 6314 387 Size: 1064 138 615 3590 374 146 1280 Fragments arranged by size: 3590 1280 1064 615 374 146 138 EcoICRI GAG'CTC Cuts at: 2677 3372 3861 2677 Size: 695 489 6023 Fragments arranged by size: 6023 695 489 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2115 2267 2918 3599 3781 3807 3845 Size: 393 483 152 651 681 182 26 38 Cuts at: 3845 3953 4159 4340 5270 5867 6654 6922 1239 Size: 108 206 181 930 597 787 268 1524 Fragments arranged by size: 1524 930 787 681 651 597 483 393 268 206 182 181 152 108 38 26 EcoNI CCTnn'n_nnAGG Cuts at: 3062 3083 4130 4214 4421 3062 Size: 21 1047 84 207 5848 Fragments arranged by size: 5848 1047 207 84 21 EcoO109I rG'GnC_Cy Cuts at: 2034 2044 3157 3238 3626 3643 3902 3928 4145 Size: 10 1113 81 388 17 259 26 217 Cuts at: 4145 4315 4491 2034 Size: 170 176 4750 Fragments arranged by size: 4750 1113 388 259 217 176 170 81 26 17 10 EcoRI G'AATT_C Cuts at: 3956 4516 4894 3956 Size: 560 378 6269 Fragments arranged by size: 6269 560 378 FspI TGC'GCA Cuts at: 2379 2513 3119 5713 2379 Size: 134 606 2594 3873 Fragments arranged by size: 3873 2594 606 134 FspAI rTGC'GCAy Cuts at: 3119 3119 Size: 7207 HaeII r_GCGC'y Cuts at: 1678 2285 2350 2744 3071 3507 4089 4436 6354 Size: 607 65 394 327 436 582 347 1918 Cuts at: 6354 1678 Size: 2531 Fragments arranged by size: 2531 1918 607 582 436 394 347 327 65 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2929 2961 3149 3181 5848 Size: 32 135 32 1357 32 188 32 2667 Cuts at: 5848 5880 5922 5954 1373 Size: 32 42 32 2626 Fragments arranged by size: 2667 2626 1357 188 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3253 4551 4797 669 Size: 988 281 1315 1298 246 3079 Fragments arranged by size: 3079 1315 1298 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7207 HpaI GTT'AAC Cuts at: 4797 4797 Size: 7207 KasI G'GCGC_C Cuts at: 2281 2346 3503 2281 Size: 65 1157 5985 Fragments arranged by size: 5985 1157 65 KpnI G_GTAC'C Cuts at: 2920 4532 2920 Size: 1612 5595 MfeI C'AATT_G Cuts at: 4806 4806 Size: 7207 MluI A'CGCG_T Cuts at: 3523 7083 3523 Size: 3560 3647 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2598 4401 6321 6505 1305 Size: 1293 1803 1920 184 2007 Fragments arranged by size: 2007 1920 1803 1293 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2942 3497 4106 10 Size: 54 1425 343 1110 555 609 3111 Fragments arranged by size: 3111 1425 1110 609 555 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2470 3329 3426 4299 5165 5524 5683 Size: 648 1304 859 97 873 866 359 159 Cuts at: 5683 518 Size: 2042 Fragments arranged by size: 2042 1304 873 866 859 648 359 159 97 MspA1I CmG'CkG Cuts at: 1606 2344 2397 2635 2806 3194 3619 3739 4153 Size: 738 53 238 171 388 425 120 414 Cuts at: 4153 4223 4623 5300 6448 6693 1606 Size: 70 400 677 1148 245 2120 Fragments arranged by size: 2120 1148 738 677 425 414 400 388 245 238 171 120 70 53 NarI GG'CG_CC Cuts at: 2282 2347 3504 2282 Size: 65 1157 5985 Fragments arranged by size: 5985 1157 65 NcoI C'CATG_G Cuts at: 513 1065 2004 4089 513 Size: 552 939 2085 3631 Fragments arranged by size: 3631 2085 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7207 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7207 NotI GC'GGCC_GC Cuts at: 4654 4654 Size: 7207 NspI r_CATG'y Cuts at: 1132 1229 2263 3049 4166 4565 6110 1132 Size: 97 1034 786 1117 399 1545 2229 Fragments arranged by size: 2229 1545 1117 1034 786 399 97 PasI CC'CwG_GG Cuts at: 1348 3178 3234 1348 Size: 1830 56 5321 Fragments arranged by size: 5321 1830 56 PciI A'CATG_T Cuts at: 6106 6106 Size: 7207 PflMI CCAn_nnn'nTGG Cuts at: 1108 4469 1108 Size: 3361 3846 PfoI T'CCnGG_A Cuts at: 2535 3645 3960 4002 2535 Size: 1110 315 42 5740 Fragments arranged by size: 5740 1110 315 42 PmeI GTTT'AAAC Cuts at: 4511 4511 Size: 7207 PmlI CAC'GTG Cuts at: 2872 3493 2872 Size: 621 6586 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2141 2173 5276 5308 6789 6821 2141 Size: 32 3103 32 1481 32 2527 Fragments arranged by size: 3103 2527 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2044 3157 3238 3643 2044 Size: 1113 81 405 5608 Fragments arranged by size: 5608 1113 405 81 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7207 PsiI TTA'TAA Cuts at: 4777 4777 Size: 7207 PspOMI G'GGCC_C Cuts at: 3927 4144 3927 Size: 217 6990 PspXI vC'TCGA_Gb Cuts at: 4130 4130 Size: 7207 PstI C_TGCA'G Cuts at: 830 2343 2748 2976 3156 3840 4188 4206 4350 Size: 1513 405 228 180 684 348 18 144 Cuts at: 4350 4392 4559 830 Size: 42 167 3478 Fragments arranged by size: 3478 1513 684 405 348 228 180 167 144 42 18 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7207 PvuII CAG'CTG Cuts at: 2806 3194 3619 3739 4153 4223 2806 Size: 388 425 120 414 70 5790 Fragments arranged by size: 5790 425 414 388 120 70 SacI G_AGCT'C Cuts at: 2679 3374 3863 2679 Size: 695 489 6023 Fragments arranged by size: 6023 695 489 SalI G'TCGA_C Cuts at: 1936 4549 1936 Size: 2613 4594 SanDI GG'GwC_CC Cuts at: 3238 3238 Size: 7207 SbfI CC_TGCA'GG Cuts at: 3156 4392 4559 3156 Size: 1236 167 5804 Fragments arranged by size: 5804 1236 167 ScaI AGT'ACT Cuts at: 5455 7167 5455 Size: 1712 5495 SfcI C'TryA_G Cuts at: 826 1045 2127 2339 2744 2972 3152 3836 4184 Size: 219 1082 212 405 228 180 684 348 Cuts at: 4184 4202 4346 4388 4555 5690 6371 6562 826 Size: 18 144 42 167 1135 681 191 1471 Fragments arranged by size: 1471 1135 1082 684 681 405 348 228 219 212 191 180 167 144 42 18 SfiI GGCCn_nnn'nGGCC Cuts at: 2808 2808 Size: 7207 SfoI GGC'GCC Cuts at: 2283 2348 3505 2283 Size: 65 1157 5985 Fragments arranged by size: 5985 1157 65 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7207 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7207 SmaI CCC'GGG Cuts at: 1872 4018 4143 4235 4534 1872 Size: 2146 125 92 299 4545 Fragments arranged by size: 4545 2146 299 125 92 SmlI C'TyrA_G Cuts at: 820 1017 1943 2480 2678 3704 4037 4130 5311 Size: 197 926 537 198 1026 333 93 1181 Cuts at: 5311 6212 6474 820 Size: 901 262 1553 Fragments arranged by size: 1553 1181 1026 926 901 537 333 262 198 197 93 SnaBI TAC'GTA Cuts at: 493 4998 493 Size: 4505 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7207 SphI G_CATG'C Cuts at: 1132 2263 4565 1132 Size: 1131 2302 3774 Fragments arranged by size: 3774 2302 1131 SspI AAT'ATT Cuts at: 5 52 5131 5 Size: 47 5079 2081 Fragments arranged by size: 5079 2081 47 StuI AGG'CCT Cuts at: 1517 4416 1517 Size: 2899 4308 StyI C'CwwG_G Cuts at: 513 1065 2004 2293 3402 3810 4089 4646 513 Size: 552 939 289 1109 408 279 557 3074 Fragments arranged by size: 3074 1109 939 557 552 408 289 279 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2843 3191 5234 5251 5404 5589 6034 Size: 1255 1263 348 2043 17 153 185 445 Cuts at: 6034 325 Size: 1498 Fragments arranged by size: 2043 1498 1263 1255 445 348 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4326 5453 7165 Size: 274 80 33 51 1443 2349 1127 1712 Cuts at: 7165 96 Size: 138 Fragments arranged by size: 2349 1712 1443 1127 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2059 2091 2183 2215 7167 7199 2059 Size: 32 92 32 4952 32 2067 Fragments arranged by size: 4952 2067 92 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7207 XbaI T'CTAG_A Cuts at: 4543 4543 Size: 7207 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3497 3497 Size: 7207 XhoI C'TCGA_G Cuts at: 1943 4130 1943 Size: 2187 5020 XmaI C'CCGG_G Cuts at: 1870 4016 4141 4233 4532 1870 Size: 2146 125 92 299 4545 Fragments arranged by size: 4545 2146 299 125 92 XmnI GAAnn'nnTTC Cuts at: 5336 5336 Size: 7207 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5015 276 Size: 53 83 186 936 3481 2468 Fragments arranged by size: 3481 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI BaeI BfrBI BsiWI BsmBI BstZ17I EcoRV FalI FseI NaeI NgoMIV NruI NsiI PacI PsrI RsrII SacII SapI SexAI SrfI SwaI