(Circular) (Six-base) MAPSORT of: pFN21AB4388.seq Check: 3872 from: 1 to: 5752 pFN21AB4388 5752 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3562 278 Size: 53 83 186 936 2026 2468 Fragments arranged by size: 2468 2026 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3095 1937 Size: 1158 4594 Acc65I G'GTAC_C Cuts at: 3073 3073 Size: 5752 AclI AA'CG_TT Cuts at: 1563 3879 4252 1563 Size: 2316 373 3063 Fragments arranged by size: 3063 2316 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2360 2683 3815 5199 5467 1632 Size: 728 323 1132 1384 268 1917 Fragments arranged by size: 1917 1384 1132 728 323 268 AfeI AGC'GCT Cuts at: 2750 2750 Size: 5752 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5555 AflIII A'CryG_T Cuts at: 1203 4651 5628 1203 Size: 3448 977 1327 Fragments arranged by size: 3448 1327 977 AgeI A'CCGG_T Cuts at: 4583 4583 Size: 5752 AhdI GACnn_n'nnGTC Cuts at: 4481 4481 Size: 5752 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5752 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5752 AlwNI CAG_nnn'CTG Cuts at: 2898 3495 5067 2898 Size: 597 1572 3583 Fragments arranged by size: 3583 1572 597 ApaLI G'TGCA_C Cuts at: 2169 3809 4965 2169 Size: 1640 1156 2956 Fragments arranged by size: 2956 1640 1156 ApoI r'AATT_y Cuts at: 1493 3061 3287 3439 5548 1493 Size: 1568 226 152 2109 1697 Fragments arranged by size: 2109 1697 1568 226 152 AseI AT'TA_AT Cuts at: 160 1032 4306 160 Size: 872 3274 1606 Fragments arranged by size: 3274 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5752 AvaI C'yCGr_G Cuts at: 1870 1943 3077 1870 Size: 73 1134 4545 Fragments arranged by size: 4545 1134 73 BamHI G'GATC_C Cuts at: 3082 3449 3082 Size: 367 5385 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3073 4528 618 Size: 325 217 627 1286 1455 1842 Fragments arranged by size: 1842 1455 1286 627 325 217 BanII G_rGCy'C Cuts at: 1391 3071 1391 Size: 1680 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2476 928 Size: 971 577 4204 Fragments arranged by size: 4204 971 577 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2836 2870 3943 3977 1903 Size: 34 899 34 1073 34 3678 Fragments arranged by size: 3678 1073 899 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3644 4854 3644 Size: 1210 4542 BclI T'GATC_A Cuts at: 1548 1548 Size: 5752 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4363 136 Size: 107 122 71 631 605 2691 1525 Fragments arranged by size: 2691 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5747 5747 Size: 5752 BlpI GC'TnA_GC Cuts at: 3169 3169 Size: 5752 Bme1580I G_kGCm'C Cuts at: 1790 2173 3813 4969 1790 Size: 383 1640 1156 2573 Fragments arranged by size: 2573 1640 1156 383 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5752 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2515 2730 3483 Size: 416 53 83 399 24 1098 215 753 Cuts at: 3483 4441 442 Size: 958 1753 Fragments arranged by size: 1753 1098 958 753 416 399 215 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5752 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2455 4412 1239 Size: 1216 1957 2579 Fragments arranged by size: 2579 1957 1216 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3877 4742 5040 3877 Size: 865 298 4589 Fragments arranged by size: 4589 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4415 882 Size: 617 2916 2219 Fragments arranged by size: 2916 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3543 493 Size: 711 2339 2702 Fragments arranged by size: 2702 2339 711 BsaBI GATnn'nnATC Cuts at: 2025 5746 2025 Size: 3721 2031 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3559 3941 4576 275 Size: 53 83 186 936 2026 382 635 1451 Fragments arranged by size: 2026 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4185 4583 4857 5004 5415 4185 Size: 398 274 147 411 4522 Fragments arranged by size: 4522 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2552 2582 1178 Size: 30 165 30 1149 30 4348 Fragments arranged by size: 4348 1149 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2261 2309 1186 Size: 1075 48 4629 Fragments arranged by size: 4629 1075 48 BseYI C'CCAG_C Cuts at: 2608 4955 5451 2608 Size: 2347 496 2909 Fragments arranged by size: 2909 2347 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2750 1163 Size: 573 1014 4165 Fragments arranged by size: 4165 1014 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3202 3963 4991 1540 Size: 275 186 1201 761 1028 2301 Fragments arranged by size: 2301 1201 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2173 3813 3898 4969 2173 Size: 1640 85 1071 2956 Fragments arranged by size: 2956 1640 1071 85 BsmI GAATG_Cn' Cuts at: 3263 3356 3263 Size: 93 5659 BsmBI CGTCTCn'nnnn_ Cuts at: 2353 2353 Size: 5752 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2173 3071 3813 3898 4969 1391 Size: 399 383 898 742 85 1071 2174 Fragments arranged by size: 2174 1071 898 742 399 383 85 BspEI T'CCGG_A Cuts at: 5415 5415 Size: 5752 BspHI T'CATG_A Cuts at: 3641 5371 3641 Size: 1730 4022 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3107 844 Size: 2263 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3639 1394 Size: 429 1816 3507 Fragments arranged by size: 3507 1816 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2388 4247 4421 65 Size: 1183 1140 1859 174 1396 Fragments arranged by size: 1859 1396 1183 1140 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4396 4583 1700 Size: 110 2586 187 2869 Fragments arranged by size: 2869 2586 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5752 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5752 BssSI C'ACGA_G Cuts at: 3812 4824 3812 Size: 1012 4740 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5752 BstBI TT'CG_AA Cuts at: 2770 4556 2770 Size: 1786 3966 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5752 BstXI CCAn_nnnn'nTGG Cuts at: 2318 2318 Size: 5752 BstYI r'GATC_y Cuts at: 1972 3082 3449 3834 3851 5303 5389 5401 5747 Size: 1110 367 385 17 1452 86 12 346 Cuts at: 5747 1972 Size: 1977 Fragments arranged by size: 1977 1452 1110 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2352 2757 513 Size: 552 1287 405 3508 Fragments arranged by size: 3508 1287 552 405 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5013 Fragments arranged by size: 5013 683 56 BtsI GCAGTG_nn' Cuts at: 3268 4062 4082 3268 Size: 794 20 4938 Fragments arranged by size: 4938 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5752 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2027 2062 2860 2895 529 Size: 35 1463 35 798 35 3386 Fragments arranged by size: 3386 1463 798 35 35 35 DraI TTT'AAA Cuts at: 3056 3056 Size: 5752 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4759 809 Size: 507 3443 1802 Fragments arranged by size: 3443 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2839 3199 4088 Size: 54 1425 50 275 18 1009 360 889 Cuts at: 4088 5717 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1009 889 360 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3199 1537 Size: 275 1387 4090 Fragments arranged by size: 4090 1387 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2336 3682 1353 Size: 983 1346 3423 Fragments arranged by size: 3423 1346 983 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4339 4713 4859 387 Size: 1064 138 2750 374 146 1280 Fragments arranged by size: 2750 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2360 2455 2683 3815 4412 5199 5467 Size: 393 728 95 228 1132 597 787 268 Cuts at: 5467 1239 Size: 1524 Fragments arranged by size: 1524 1132 787 728 597 393 268 228 95 EcoO109I rG'GnC_Cy Cuts at: 3029 3029 Size: 5752 EcoRI G'AATT_C Cuts at: 3061 3439 3061 Size: 378 5374 EcoRV GAT'ATC Cuts at: 2522 2677 2522 Size: 155 5597 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3050 3050 Size: 5752 FspI TGC'GCA Cuts at: 4258 4258 Size: 5752 HaeII r_GCGC'y Cuts at: 1678 2752 2956 4899 1678 Size: 1074 204 1943 2531 Fragments arranged by size: 2531 1943 1074 204 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3033 3065 4393 4425 4467 Size: 32 135 32 1461 32 1328 32 42 Cuts at: 4467 4499 1373 Size: 32 2626 Fragments arranged by size: 2626 1461 1328 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3096 3342 669 Size: 988 281 1158 246 3079 Fragments arranged by size: 3079 1158 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5752 HpaI GTT'AAC Cuts at: 3342 3342 Size: 5752 KpnI G_GTAC'C Cuts at: 3077 3077 Size: 5752 MfeI C'AATT_G Cuts at: 3351 3351 Size: 5752 MluI A'CGCG_T Cuts at: 5628 5628 Size: 5752 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2805 2949 4866 5050 1305 Size: 1500 144 1917 184 2007 Fragments arranged by size: 2007 1917 1500 184 144 MscI TGG'CCA Cuts at: 10 64 1489 1832 2841 10 Size: 54 1425 343 1009 2921 Fragments arranged by size: 2921 1425 1009 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2147 2529 2591 3710 4069 4228 518 Size: 648 981 382 62 1119 359 159 2042 Fragments arranged by size: 2042 1119 981 648 382 359 159 62 MspA1I CmG'CkG Cuts at: 1606 3168 3845 4993 5238 1606 Size: 1562 677 1148 245 2120 Fragments arranged by size: 2120 1562 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2352 2757 513 Size: 552 1287 405 3508 Fragments arranged by size: 3508 1287 552 405 NdeI CA'TA_TG Cuts at: 387 2221 387 Size: 1834 3918 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5752 NotI GC'GGCC_GC Cuts at: 3199 3199 Size: 5752 NspI r_CATG'y Cuts at: 1132 1229 3110 4655 1132 Size: 97 1881 1545 2229 Fragments arranged by size: 2229 1881 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5752 PciI A'CATG_T Cuts at: 4651 4651 Size: 5752 PflMI CCAn_nnn'nTGG Cuts at: 1108 2215 1108 Size: 1107 4645 PmeI GTTT'AAAC Cuts at: 3056 3056 Size: 5752 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3821 3853 5334 5366 3821 Size: 32 1481 32 4207 Fragments arranged by size: 4207 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2480 1613 Size: 867 4885 PsiI TTA'TAA Cuts at: 3322 3322 Size: 5752 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2319 2351 2319 Size: 32 5720 PstI C_TGCA'G Cuts at: 830 3104 830 Size: 2274 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5752 SalI G'TCGA_C Cuts at: 1936 3094 1936 Size: 1158 4594 SapI GCTCTTCn'nnn_ Cuts at: 2336 2336 Size: 5752 SbfI CC_TGCA'GG Cuts at: 3104 3104 Size: 5752 ScaI AGT'ACT Cuts at: 4000 5712 4000 Size: 1712 4040 SfcI C'TryA_G Cuts at: 826 1045 2994 3100 4235 4916 5107 826 Size: 219 1949 106 1135 681 191 1471 Fragments arranged by size: 1949 1471 1135 681 219 191 106 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5752 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5752 SmaI CCC'GGG Cuts at: 1872 3079 1872 Size: 1207 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3856 4757 5019 820 Size: 197 926 1913 901 262 1553 Fragments arranged by size: 1913 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3543 493 Size: 3050 2702 SpeI A'CTAG_T Cuts at: 152 2777 152 Size: 2625 3127 SphI G_CATG'C Cuts at: 1132 3110 1132 Size: 1978 3774 SspI AAT'ATT Cuts at: 5 52 3676 5 Size: 47 3624 2081 Fragments arranged by size: 3624 2081 47 StuI AGG'CCT Cuts at: 1517 2735 1517 Size: 1218 4534 StyI C'CwwG_G Cuts at: 513 1065 2016 2352 2757 3191 513 Size: 552 951 336 405 434 3074 Fragments arranged by size: 3074 951 552 434 405 336 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3779 3796 3949 4134 4579 325 Size: 1255 2199 17 153 185 445 1498 Fragments arranged by size: 2199 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2972 3998 5710 Size: 274 80 33 51 1443 995 1026 1712 Cuts at: 5710 96 Size: 138 Fragments arranged by size: 1712 1443 1026 995 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2552 2584 5712 5744 2552 Size: 32 3128 32 2560 Fragments arranged by size: 3128 2560 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5752 XbaI T'CTAG_A Cuts at: 3088 3088 Size: 5752 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2359 2359 Size: 5752 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5752 XmaI C'CCGG_G Cuts at: 1870 3077 1870 Size: 1207 4545 XmnI GAAnn'nnTTC Cuts at: 3881 3881 Size: 5752 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3560 276 Size: 53 83 186 936 2026 2468 Fragments arranged by size: 2468 2026 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI