(Circular) (Six-base) MAPSORT of: pFN21AB4387.seq Check: 893 from: 1 to: 7279 pFN21AB4387 7279 bp With 182 enzymes: SgfI * October 20, 2009 10:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2213 2489 3101 2213 Size: 276 612 6391 Fragments arranged by size: 6391 612 276 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5089 278 Size: 53 83 186 936 3553 2468 Fragments arranged by size: 3553 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4622 1937 Size: 2685 4594 Acc65I G'GTAC_C Cuts at: 4600 4600 Size: 7279 AclI AA'CG_TT Cuts at: 1563 3533 5406 5779 1563 Size: 1970 1873 373 3063 Fragments arranged by size: 3063 1970 1873 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2206 5342 6726 6994 1632 Size: 574 3136 1384 268 1917 Fragments arranged by size: 3136 1917 1384 574 268 AfeI AGC'GCT Cuts at: 2152 2552 2152 Size: 400 6879 AflII C'TTAA_G Cuts at: 820 1017 2999 4448 820 Size: 197 1982 1449 3651 Fragments arranged by size: 3651 1982 1449 197 AflIII A'CryG_T Cuts at: 1203 2568 6178 7155 1203 Size: 1365 3610 977 1327 Fragments arranged by size: 3610 1365 1327 977 AgeI A'CCGG_T Cuts at: 6110 6110 Size: 7279 AhdI GACnn_n'nnGTC Cuts at: 6008 6008 Size: 7279 AleI CACnn'nnGTG Cuts at: 1166 3308 4286 1166 Size: 2142 978 4159 Fragments arranged by size: 4159 2142 978 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7279 AlwNI CAG_nnn'CTG Cuts at: 4042 4148 5022 6594 4042 Size: 106 874 1572 4727 Fragments arranged by size: 4727 1572 874 106 ApaI G_GGCC'C Cuts at: 2013 2013 Size: 7279 ApaLI G'TGCA_C Cuts at: 5336 6492 5336 Size: 1156 6123 ApoI r'AATT_y Cuts at: 1493 2723 4588 4814 4966 7075 1493 Size: 1230 1865 226 152 2109 1697 Fragments arranged by size: 2109 1865 1697 1230 226 152 AseI AT'TA_AT Cuts at: 160 1032 5833 160 Size: 872 4801 1606 Fragments arranged by size: 4801 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7279 AvaI C'yCGr_G Cuts at: 1870 1943 4604 1870 Size: 73 2661 4545 Fragments arranged by size: 4545 2661 73 BamHI G'GATC_C Cuts at: 3082 3453 3577 4609 4976 3082 Size: 371 124 1032 367 5385 Fragments arranged by size: 5385 1032 371 367 124 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4600 6055 618 Size: 325 217 627 2813 1455 1842 Fragments arranged by size: 2813 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2013 3194 3382 4281 4372 4598 1391 Size: 622 1181 188 899 91 226 4072 Fragments arranged by size: 4072 1181 899 622 226 188 91 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3797 4396 4414 4499 928 Size: 971 1898 599 18 85 3708 Fragments arranged by size: 3708 1898 971 599 85 18 BbvCI CC'TCA_GC Cuts at: 4149 4149 Size: 7279 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5470 5504 1903 Size: 34 3533 34 3678 Fragments arranged by size: 3678 3533 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3502 5171 6381 3502 Size: 1669 1210 4400 Fragments arranged by size: 4400 1669 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7279 BfrBI ATG'CAT Cuts at: 2121 2871 2121 Size: 750 6529 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2166 2622 3030 Size: 107 122 71 631 605 494 456 408 Cuts at: 3030 3182 4104 5890 136 Size: 152 922 1786 1525 Fragments arranged by size: 1786 1525 922 631 605 494 456 408 152 122 107 71 BglII A'GATC_T Cuts at: 3709 7274 3709 Size: 3565 3714 BlpI GC'TnA_GC Cuts at: 4696 4696 Size: 7279 Bme1580I G_kGCm'C Cuts at: 1790 2013 3026 3494 5340 6496 1790 Size: 223 1013 468 1846 1156 2573 Fragments arranged by size: 2573 1846 1156 1013 468 223 BmgBI CAC'GTC Cuts at: 1318 2190 2328 2986 4573 1318 Size: 872 138 658 1587 4024 Fragments arranged by size: 4024 1587 872 658 138 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3619 3742 4350 Size: 416 53 83 399 24 2202 123 608 Cuts at: 4350 5010 5968 442 Size: 660 958 1753 Fragments arranged by size: 2202 1753 958 660 608 416 399 123 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7279 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3568 3568 Size: 7279 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2284 2623 3137 4583 5939 1239 Size: 1045 339 514 1446 1356 2579 Fragments arranged by size: 2579 1446 1356 1045 514 339 Bpu10I CC'TnA_GC Cuts at: 4019 4149 4019 Size: 130 7149 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3745 5404 6269 6567 3745 Size: 1659 865 298 4457 Fragments arranged by size: 4457 1659 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5942 882 Size: 617 4443 2219 Fragments arranged by size: 4443 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2569 5070 493 Size: 711 1365 2501 2702 Fragments arranged by size: 2702 2501 1365 711 BsaBI GATnn'nnATC Cuts at: 7273 7273 Size: 7279 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3387 5086 5468 6103 Size: 53 83 186 936 1854 1699 382 635 Cuts at: 6103 275 Size: 1451 Fragments arranged by size: 1854 1699 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3249 3786 5712 6110 6384 6531 6942 3249 Size: 537 1926 398 274 147 411 3586 Fragments arranged by size: 3586 1926 537 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2411 2441 3815 3845 1178 Size: 30 165 30 1008 30 1374 30 4612 Fragments arranged by size: 4612 1374 1008 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2039 3704 4005 4144 4388 1186 Size: 853 1665 301 139 244 4077 Fragments arranged by size: 4077 1665 853 301 244 139 BseYI C'CCAG_C Cuts at: 2490 3048 3366 3981 6482 6978 2490 Size: 558 318 615 2501 496 2791 Fragments arranged by size: 2791 2501 615 558 496 318 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6706 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4729 5490 6518 1540 Size: 275 186 2728 761 1028 2301 Fragments arranged by size: 2728 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2333 2442 2735 4398 5340 5425 6496 2333 Size: 109 293 1663 942 85 1071 3116 Fragments arranged by size: 3116 1663 1071 942 293 109 85 BsmI GAATG_Cn' Cuts at: 2250 2873 2960 4790 4883 2250 Size: 623 87 1830 93 4646 Fragments arranged by size: 4646 1830 623 93 87 BsmBI CGTCTCn'nnnn_ Cuts at: 2180 3394 2180 Size: 1214 6065 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2333 2442 2735 3026 3194 3382 Size: 399 223 320 109 293 291 168 188 Cuts at: 3382 3494 4281 4372 4398 4598 5340 5425 6496 Size: 112 787 91 26 200 742 85 1071 Cuts at: 6496 1391 Size: 2174 Fragments arranged by size: 2174 1071 787 742 399 320 293 291 223 200 188 168 112 109 91 85 26 BspEI T'CCGG_A Cuts at: 6942 6942 Size: 7279 BspHI T'CATG_A Cuts at: 5168 6898 5168 Size: 1730 5549 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2213 2489 3101 4097 4634 844 Size: 1369 276 612 996 537 3489 Fragments arranged by size: 3489 1369 996 612 537 276 BsrBI CCG'CTC Cuts at: 1394 1823 5166 1394 Size: 429 3343 3507 Fragments arranged by size: 3507 3343 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2998 4030 5774 5948 65 Size: 1183 1750 1032 1744 174 1396 Fragments arranged by size: 1750 1744 1396 1183 1032 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2760 3219 5923 6110 1700 Size: 110 950 459 2704 187 2869 Fragments arranged by size: 2869 2704 950 459 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7279 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7279 BssSI C'ACGA_G Cuts at: 3224 5339 6351 3224 Size: 2115 1012 4152 Fragments arranged by size: 4152 2115 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7279 BstBI TT'CG_AA Cuts at: 6083 6083 Size: 7279 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7279 BstXI CCAn_nnnn'nTGG Cuts at: 3574 3574 Size: 7279 BstYI r'GATC_y Cuts at: 1972 3082 3453 3577 3709 4609 4976 5361 5378 Size: 1110 371 124 132 900 367 385 17 Cuts at: 5378 6830 6916 6928 7274 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1110 900 385 371 367 346 132 124 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3281 3281 Size: 7279 BtgI C'CryG_G Cuts at: 513 1065 2004 2055 2752 3382 4284 513 Size: 552 939 51 697 630 902 3508 Fragments arranged by size: 3508 939 902 697 630 552 51 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2157 2286 2814 3181 487 Size: 683 56 931 129 528 367 4585 Fragments arranged by size: 4585 931 683 528 367 129 56 BtsI GCAGTG_nn' Cuts at: 2076 2611 2771 4164 4795 5589 5609 2076 Size: 535 160 1393 631 794 20 3746 Fragments arranged by size: 3746 1393 794 631 535 160 20 ClaI AT'CG_AT Cuts at: 1554 3069 3768 1554 Size: 1515 699 5065 Fragments arranged by size: 5065 1515 699 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7244 DraI TTT'AAA Cuts at: 4583 4583 Size: 7279 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2194 4481 6286 809 Size: 507 878 2287 1805 1802 Fragments arranged by size: 2287 1805 1802 878 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2275 2543 2703 Size: 54 1425 50 275 18 445 268 160 Cuts at: 2703 3312 3721 3837 4116 4161 4726 5615 7244 Size: 609 409 116 279 45 565 889 1629 Cuts at: 7244 8 Size: 43 Fragments arranged by size: 1629 1425 889 609 565 445 409 279 275 268 160 116 54 50 45 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4726 1537 Size: 275 2914 4090 Fragments arranged by size: 4090 2914 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3212 3397 3518 3971 5209 1353 Size: 1859 185 121 453 1238 3423 Fragments arranged by size: 3423 1859 1238 453 185 121 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5866 6240 6386 387 Size: 1064 138 4277 374 146 1280 Fragments arranged by size: 4277 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2206 2284 2623 3137 4583 5342 5939 Size: 393 574 78 339 514 1446 759 597 Cuts at: 5939 6726 6994 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1446 787 759 597 574 514 393 339 268 78 EcoNI CCTnn'n_nnAGG Cuts at: 4142 4142 Size: 7279 EcoO109I rG'GnC_Cy Cuts at: 2009 2010 2837 3427 3640 2009 Size: 1 827 590 213 5648 Fragments arranged by size: 5648 827 590 213 1 EcoRI G'AATT_C Cuts at: 2723 4588 4966 2723 Size: 1865 378 5036 Fragments arranged by size: 5036 1865 378 EcoRV GAT'ATC Cuts at: 3067 3871 3067 Size: 804 6475 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3814 3814 Size: 7279 FspI TGC'GCA Cuts at: 5785 5785 Size: 7279 HaeII r_GCGC'y Cuts at: 1678 2154 2554 2650 6426 1678 Size: 476 400 96 3776 2531 Fragments arranged by size: 3776 2531 476 400 96 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2282 2314 2411 2443 3647 Size: 32 135 32 710 32 97 32 1204 Cuts at: 3647 3679 4291 4323 4543 4575 5920 5952 5994 Size: 32 612 32 220 32 1345 32 42 Cuts at: 5994 6026 1373 Size: 32 2626 Fragments arranged by size: 2626 1345 1204 710 612 220 135 97 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2242 3739 3775 3922 4623 4869 Size: 988 281 304 1497 36 147 701 246 Cuts at: 4869 669 Size: 3079 Fragments arranged by size: 3079 1497 988 701 304 281 246 147 36 HindIII A'AGCT_T Cuts at: 748 748 Size: 7279 HpaI GTT'AAC Cuts at: 4869 4869 Size: 7279 KpnI G_GTAC'C Cuts at: 4604 4604 Size: 7279 MfeI C'AATT_G Cuts at: 4878 4878 Size: 7279 MluI A'CGCG_T Cuts at: 7155 7155 Size: 7279 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6393 6577 1305 Size: 5088 184 2007 Fragments arranged by size: 5088 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2277 2705 3314 4118 4163 Size: 54 1425 343 445 428 609 804 45 Cuts at: 4163 10 Size: 3126 Fragments arranged by size: 3126 1425 804 609 445 428 343 54 45 MslI CAynn'nnrTG Cuts at: 518 1166 3308 3572 4286 5237 5596 5755 518 Size: 648 2142 264 714 951 359 159 2042 Fragments arranged by size: 2142 2042 951 714 648 359 264 159 MspA1I CmG'CkG Cuts at: 1606 2057 2226 2247 2494 2643 2862 3040 3157 Size: 451 169 21 247 149 219 178 117 Cuts at: 3157 4695 5372 6520 6765 1606 Size: 1538 677 1148 245 2120 Fragments arranged by size: 2120 1538 1148 677 451 247 245 219 178 169 149 117 21 NaeI GCC'GGC Cuts at: 2762 2762 Size: 7279 NcoI C'CATG_G Cuts at: 513 1065 2004 2752 513 Size: 552 939 748 5040 Fragments arranged by size: 5040 939 748 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7279 NgoMIV G'CCGG_C Cuts at: 2760 2760 Size: 7279 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7279 NotI GC'GGCC_GC Cuts at: 4726 4726 Size: 7279 NsiI A_TGCA'T Cuts at: 2123 2873 2123 Size: 750 6529 NspI r_CATG'y Cuts at: 1132 1229 4111 4637 6182 1132 Size: 97 2882 526 1545 2229 Fragments arranged by size: 2882 2229 1545 526 97 PasI CC'CwG_GG Cuts at: 1348 2013 4274 1348 Size: 665 2261 4353 Fragments arranged by size: 4353 2261 665 PciI A'CATG_T Cuts at: 6178 6178 Size: 7279 PflMI CCAn_nnn'nTGG Cuts at: 1108 3043 4318 1108 Size: 1935 1275 4069 Fragments arranged by size: 4069 1935 1275 PmeI GTTT'AAAC Cuts at: 4583 4583 Size: 7279 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5348 5380 6861 6893 5348 Size: 32 1481 32 5734 Fragments arranged by size: 5734 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2837 2837 Size: 7279 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7279 PsiI TTA'TAA Cuts at: 4849 4849 Size: 7279 PspOMI G'GGCC_C Cuts at: 2009 2009 Size: 7279 PstI C_TGCA'G Cuts at: 830 2071 3098 3362 4159 4631 830 Size: 1241 1027 264 797 472 3478 Fragments arranged by size: 3478 1241 1027 797 472 264 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7279 PvuII CAG'CTG Cuts at: 2226 2494 2643 3040 3157 2226 Size: 268 149 397 117 6348 Fragments arranged by size: 6348 397 268 149 117 SacII CC_GC'GG Cuts at: 2058 2058 Size: 7279 SalI G'TCGA_C Cuts at: 1936 4621 1936 Size: 2685 4594 SanDI GG'GwC_CC Cuts at: 2837 2837 Size: 7279 SbfI CC_TGCA'GG Cuts at: 4631 4631 Size: 7279 ScaI AGT'ACT Cuts at: 5527 7239 5527 Size: 1712 5567 SfcI C'TryA_G Cuts at: 826 1045 2067 2554 3094 3358 4155 4537 4627 Size: 219 1022 487 540 264 797 382 90 Cuts at: 4627 5762 6443 6634 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1022 797 681 540 487 382 264 219 191 90 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7279 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7279 SmaI CCC'GGG Cuts at: 1872 4606 1872 Size: 2734 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2999 3760 4448 5383 6284 6546 Size: 197 926 1056 761 688 935 901 262 Cuts at: 6546 820 Size: 1553 Fragments arranged by size: 1553 1056 935 926 901 761 688 262 197 SnaBI TAC'GTA Cuts at: 493 5070 493 Size: 4577 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7279 SphI G_CATG'C Cuts at: 1132 4111 4637 1132 Size: 2979 526 3774 Fragments arranged by size: 3774 2979 526 SspI AAT'ATT Cuts at: 5 52 5203 5 Size: 47 5151 2081 Fragments arranged by size: 5151 2081 47 StuI AGG'CCT Cuts at: 1517 2426 3176 1517 Size: 909 750 5620 Fragments arranged by size: 5620 909 750 StyI C'CwwG_G Cuts at: 513 1065 2004 2575 2752 3513 3560 3834 4718 Size: 552 939 571 177 761 47 274 884 Cuts at: 4718 513 Size: 3074 Fragments arranged by size: 3074 939 884 761 571 552 274 177 47 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3380 3491 3995 4181 5306 5323 5476 Size: 1255 1800 111 504 186 1125 17 153 Cuts at: 5476 5661 6106 325 Size: 185 445 1498 Fragments arranged by size: 1800 1498 1255 1125 504 445 186 185 153 111 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3258 3597 5525 Size: 274 80 33 51 1443 1281 339 1928 Cuts at: 5525 7237 96 Size: 1712 138 Fragments arranged by size: 1928 1712 1443 1281 339 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2434 2466 3815 3847 7239 7271 2434 Size: 32 1349 32 3392 32 2442 Fragments arranged by size: 3392 2442 1349 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2885 3404 3482 4303 1316 Size: 1569 519 78 821 4292 Fragments arranged by size: 4292 1569 821 519 78 XbaI T'CTAG_A Cuts at: 4615 4615 Size: 7279 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2618 2735 2956 2618 Size: 117 221 6941 Fragments arranged by size: 6941 221 117 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7279 XmaI C'CCGG_G Cuts at: 1870 4604 1870 Size: 2734 4545 XmnI GAAnn'nnTTC Cuts at: 3681 5408 3681 Size: 1727 5552 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5087 276 Size: 53 83 186 936 3553 2468 Fragments arranged by size: 3553 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BsiWI BstZ17I DraIII EcoICRI FseI FspAI KasI NarI NruI PacI PfoI PmlI PspXI PsrI RsrII SacI SapI SexAI SfiI SfoI SrfI SwaI