(Circular) (Six-base) MAPSORT of: pFN21AB4380.seq Check: 7346 from: 1 to: 5878 pFN21AB4380 5878 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3161 3161 Size: 5878 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3688 278 Size: 53 83 186 936 2152 2468 Fragments arranged by size: 2468 2152 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3221 1937 Size: 1284 4594 Acc65I G'GTAC_C Cuts at: 3199 3199 Size: 5878 AclI AA'CG_TT Cuts at: 1563 4005 4378 1563 Size: 2442 373 3063 Fragments arranged by size: 3063 2442 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3941 5325 5593 1632 Size: 2309 1384 268 1917 Fragments arranged by size: 2309 1917 1384 268 AfeI AGC'GCT Cuts at: 2795 2999 2795 Size: 204 5674 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5681 AflIII A'CryG_T Cuts at: 1203 2424 2886 4777 5754 1203 Size: 1221 462 1891 977 1327 Fragments arranged by size: 1891 1327 1221 977 462 AgeI A'CCGG_T Cuts at: 4709 4709 Size: 5878 AhdI GACnn_n'nnGTC Cuts at: 4607 4607 Size: 5878 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5878 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5878 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3110 3142 3110 Size: 32 5846 AlwNI CAG_nnn'CTG Cuts at: 3621 5193 3621 Size: 1572 4306 ApaI G_GGCC'C Cuts at: 2093 2093 Size: 5878 ApaLI G'TGCA_C Cuts at: 3935 5091 3935 Size: 1156 4722 ApoI r'AATT_y Cuts at: 1493 3187 3413 3565 5674 1493 Size: 1694 226 152 2109 1697 Fragments arranged by size: 2109 1697 1694 226 152 AseI AT'TA_AT Cuts at: 160 1032 4432 160 Size: 872 3400 1606 Fragments arranged by size: 3400 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5878 AvaI C'yCGr_G Cuts at: 1870 1943 2056 2093 3203 1870 Size: 73 113 37 1110 4545 Fragments arranged by size: 4545 1110 113 73 37 BamHI G'GATC_C Cuts at: 3208 3575 3208 Size: 367 5511 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2018 3149 3199 4654 618 Size: 325 217 627 231 1131 50 1455 1842 Fragments arranged by size: 1842 1455 1131 627 325 231 217 50 BanII G_rGCy'C Cuts at: 1391 2093 2649 3197 1391 Size: 702 556 548 4072 Fragments arranged by size: 4072 702 556 548 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2279 2578 3108 3130 928 Size: 971 380 299 530 22 3676 Fragments arranged by size: 3676 971 530 380 299 22 BbvCI CC'TCA_GC Cuts at: 3023 3023 Size: 5878 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4069 4103 1903 Size: 34 2132 34 3678 Fragments arranged by size: 3678 2132 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3770 4980 3770 Size: 1210 4668 BclI T'GATC_A Cuts at: 1548 1548 Size: 5878 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2771 4489 136 Size: 107 122 71 631 605 1099 1718 1525 Fragments arranged by size: 1718 1525 1099 631 605 122 107 71 BglII A'GATC_T Cuts at: 5873 5873 Size: 5878 BlpI GC'TnA_GC Cuts at: 2330 2405 2456 2895 3295 2330 Size: 75 51 439 400 4913 Fragments arranged by size: 4913 439 400 75 51 Bme1580I G_kGCm'C Cuts at: 1790 2093 2340 3939 5095 1790 Size: 303 247 1599 1156 2573 Fragments arranged by size: 2573 1599 1156 303 247 BmgBI CAC'GTC Cuts at: 1318 3159 1318 Size: 1841 4037 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3609 4567 442 Size: 416 53 83 399 24 2192 958 1753 Fragments arranged by size: 2192 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5878 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3143 4538 1239 Size: 1904 1395 2579 Fragments arranged by size: 2579 1904 1395 Bpu10I CC'TnA_GC Cuts at: 3023 3023 Size: 5878 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2176 2753 3062 4003 4868 5166 2176 Size: 577 309 941 865 298 2888 Fragments arranged by size: 2888 941 865 577 309 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4541 882 Size: 617 3042 2219 Fragments arranged by size: 3042 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2248 2473 2734 2869 3669 493 Size: 711 1044 225 261 135 800 2702 Fragments arranged by size: 2702 1044 800 711 261 225 135 BsaBI GATnn'nnATC Cuts at: 5872 5872 Size: 5878 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2907 3135 3685 4067 Size: 53 83 186 936 1374 228 550 382 Cuts at: 4067 4702 275 Size: 635 1451 Fragments arranged by size: 1451 1374 936 635 550 382 228 186 83 53 BsaWI w'CCGG_w Cuts at: 4311 4709 4983 5130 5541 4311 Size: 398 274 147 411 4648 Fragments arranged by size: 4648 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5653 Fragments arranged by size: 5653 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2017 2029 2135 3062 1186 Size: 831 12 106 927 4002 Fragments arranged by size: 4002 927 831 106 12 BseYI C'CCAG_C Cuts at: 2051 5081 5577 2051 Size: 3030 496 2352 Fragments arranged by size: 3030 2352 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2894 3139 1163 Size: 573 1158 245 3902 Fragments arranged by size: 3902 1158 573 245 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3328 4089 5117 1540 Size: 275 186 1327 761 1028 2301 Fragments arranged by size: 2301 1327 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2406 2457 2739 3939 4024 5095 2406 Size: 51 282 1200 85 1071 3189 Fragments arranged by size: 3189 1200 1071 282 85 51 BsmI GAATG_Cn' Cuts at: 3389 3482 3389 Size: 93 5785 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2093 2340 2406 2457 2649 2739 3197 Size: 399 303 247 66 51 192 90 458 Cuts at: 3197 3939 4024 5095 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 458 399 303 247 192 90 85 66 51 BspEI T'CCGG_A Cuts at: 5541 5541 Size: 5878 BspHI T'CATG_A Cuts at: 3767 5497 3767 Size: 1730 4148 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3161 3233 844 Size: 2317 72 3489 Fragments arranged by size: 3489 2317 72 BsrBI CCG'CTC Cuts at: 1394 1823 2651 3765 1394 Size: 429 828 1114 3507 Fragments arranged by size: 3507 1114 828 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2307 2742 4373 4547 65 Size: 1183 1059 435 1631 174 1396 Fragments arranged by size: 1631 1396 1183 1059 435 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2099 2511 2774 3052 4522 4709 1700 Size: 110 289 412 263 278 1470 187 2869 Fragments arranged by size: 2869 1470 412 289 278 263 187 110 BsrGI T'GTAC_A Cuts at: 96 2679 96 Size: 2583 3295 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5878 BssSI C'ACGA_G Cuts at: 2399 3938 4950 2399 Size: 1539 1012 3327 Fragments arranged by size: 3327 1539 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3018 794 Size: 2224 3654 BstBI TT'CG_AA Cuts at: 4682 4682 Size: 5878 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5878 BstYI r'GATC_y Cuts at: 1972 3208 3575 3960 3977 5429 5515 5527 5873 Size: 1236 367 385 17 1452 86 12 346 Cuts at: 5873 1972 Size: 1977 Fragments arranged by size: 1977 1452 1236 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2202 2478 2520 2598 2709 2910 3042 Size: 552 1137 276 42 78 111 201 132 Cuts at: 3042 513 Size: 3349 Fragments arranged by size: 3349 1137 552 276 201 132 111 78 42 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5139 Fragments arranged by size: 5139 683 56 BtsI GCAGTG_nn' Cuts at: 3394 4188 4208 3394 Size: 794 20 5064 Fragments arranged by size: 5064 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5878 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5843 DraI TTT'AAA Cuts at: 3182 3182 Size: 5878 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4885 809 Size: 507 3569 1802 Fragments arranged by size: 3569 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2277 2304 2475 Size: 54 1425 50 275 18 447 27 171 Cuts at: 2475 2523 2601 3325 4214 5843 8 Size: 48 78 724 889 1629 43 Fragments arranged by size: 1629 1425 889 724 447 275 171 78 54 50 48 43 27 18 EagI C'GGCC_G Cuts at: 1537 1812 3325 1537 Size: 275 1513 4090 Fragments arranged by size: 4090 1513 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3808 1353 Size: 2455 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4465 4839 4985 387 Size: 1064 138 2876 374 146 1280 Fragments arranged by size: 2876 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3143 3941 4538 5325 5593 1239 Size: 393 1511 798 597 787 268 1524 Fragments arranged by size: 1524 1511 798 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2089 2090 2151 2089 Size: 1 61 5816 Fragments arranged by size: 5816 61 1 EcoRI G'AATT_C Cuts at: 3187 3565 3187 Size: 378 5500 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2830 2830 Size: 5878 FspI TGC'GCA Cuts at: 2756 4384 2756 Size: 1628 4250 FspAI rTGC'GCAy Cuts at: 2756 2756 Size: 5878 HaeII r_GCGC'y Cuts at: 1678 2106 2178 2797 3001 5025 1678 Size: 428 72 619 204 2024 2531 Fragments arranged by size: 2531 2024 619 428 204 72 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4519 4551 4593 4625 1373 Size: 32 135 32 2947 32 42 32 2626 Fragments arranged by size: 2947 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2422 2884 3058 3222 3468 669 Size: 988 281 484 462 174 164 246 3079 Fragments arranged by size: 3079 988 484 462 281 246 174 164 HindIII A'AGCT_T Cuts at: 748 748 Size: 5878 HpaI GTT'AAC Cuts at: 3468 3468 Size: 5878 KpnI G_GTAC'C Cuts at: 3203 3203 Size: 5878 MfeI C'AATT_G Cuts at: 3477 3477 Size: 5878 MluI A'CGCG_T Cuts at: 5754 5754 Size: 5878 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3031 3133 4992 5176 1305 Size: 1726 102 1859 184 2007 Fragments arranged by size: 2007 1859 1726 184 102 MscI TGG'CCA Cuts at: 10 64 1489 1832 2306 2525 10 Size: 54 1425 343 474 219 3363 Fragments arranged by size: 3363 1425 474 343 219 54 MslI CAynn'nnrTG Cuts at: 518 1166 2246 2636 2816 3836 4195 4354 518 Size: 648 1080 390 180 1020 359 159 2042 Fragments arranged by size: 2042 1080 1020 648 390 359 180 159 MspA1I CmG'CkG Cuts at: 1606 2064 2600 2878 2923 2952 3027 3044 3294 Size: 458 536 278 45 29 75 17 250 Cuts at: 3294 3971 5119 5364 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 536 458 278 250 245 75 45 29 17 NaeI GCC'GGC Cuts at: 2101 2513 2776 2101 Size: 412 263 5203 Fragments arranged by size: 5203 412 263 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5326 NdeI CA'TA_TG Cuts at: 387 387 Size: 5878 NgoMIV G'CCGG_C Cuts at: 2099 2511 2774 2099 Size: 412 263 5203 Fragments arranged by size: 5203 412 263 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5878 NotI GC'GGCC_GC Cuts at: 3325 3325 Size: 5878 NspI r_CATG'y Cuts at: 1132 1229 2428 2635 3236 4781 1132 Size: 97 1199 207 601 1545 2229 Fragments arranged by size: 2229 1545 1199 601 207 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5878 PciI A'CATG_T Cuts at: 2424 4777 2424 Size: 2353 3525 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5878 PfoI T'CCnGG_A Cuts at: 3161 3161 Size: 5878 PmeI GTTT'AAAC Cuts at: 3182 3182 Size: 5878 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3947 3979 5460 5492 3947 Size: 32 1481 32 4333 Fragments arranged by size: 4333 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2151 2151 Size: 5878 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5878 PsiI TTA'TAA Cuts at: 3448 3448 Size: 5878 PspOMI G'GGCC_C Cuts at: 2089 2089 Size: 5878 PstI C_TGCA'G Cuts at: 830 3230 830 Size: 2400 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5878 PvuII CAG'CTG Cuts at: 2064 2878 2923 2952 3027 2064 Size: 814 45 29 75 4915 Fragments arranged by size: 4915 814 75 45 29 SacII CC_GC'GG Cuts at: 2601 3045 2601 Size: 444 5434 SalI G'TCGA_C Cuts at: 1936 3220 1936 Size: 1284 4594 SbfI CC_TGCA'GG Cuts at: 3230 3230 Size: 5878 ScaI AGT'ACT Cuts at: 4126 5838 4126 Size: 1712 4166 SfcI C'TryA_G Cuts at: 826 1045 3091 3226 4361 5042 5233 826 Size: 219 2046 135 1135 681 191 1471 Fragments arranged by size: 2046 1471 1135 681 219 191 135 SfiI GGCCn_nnn'nGGCC Cuts at: 2771 2771 Size: 5878 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5878 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5878 SmaI CCC'GGG Cuts at: 1872 2058 2095 3205 1872 Size: 186 37 1110 4545 Fragments arranged by size: 4545 1110 186 37 SmlI C'TyrA_G Cuts at: 820 1017 1943 2155 2768 3077 3982 4883 5145 Size: 197 926 212 613 309 905 901 262 Cuts at: 5145 820 Size: 1553 Fragments arranged by size: 1553 926 905 901 613 309 262 212 197 SnaBI TAC'GTA Cuts at: 493 3669 493 Size: 3176 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5878 SphI G_CATG'C Cuts at: 1132 3236 1132 Size: 2104 3774 SrfI GCCC'GGGC Cuts at: 2058 2058 Size: 5878 SspI AAT'ATT Cuts at: 5 52 3802 5 Size: 47 3750 2081 Fragments arranged by size: 3750 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5878 StyI C'CwwG_G Cuts at: 513 1065 2526 3317 513 Size: 552 1461 791 3074 Fragments arranged by size: 3074 1461 791 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2539 2917 3905 3922 4075 4260 4705 Size: 1255 959 378 988 17 153 185 445 Cuts at: 4705 325 Size: 1498 Fragments arranged by size: 1498 1255 988 959 445 378 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2679 4124 5836 Size: 274 80 33 51 1443 702 1445 1712 Cuts at: 5836 96 Size: 138 Fragments arranged by size: 1712 1445 1443 702 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5838 5870 5838 Size: 32 5846 Tth111I GACn'n_nGTC Cuts at: 1316 2417 1316 Size: 1101 4777 XbaI T'CTAG_A Cuts at: 3214 3214 Size: 5878 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5878 XmaI C'CCGG_G Cuts at: 1870 2056 2093 3203 1870 Size: 186 37 1110 4545 Fragments arranged by size: 4545 1110 186 37 XmnI GAAnn'nnTTC Cuts at: 4007 4007 Size: 5878 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3686 276 Size: 53 83 186 936 2152 2468 Fragments arranged by size: 2468 2152 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SanDI SapI SexAI SfoI SwaI XcmI