Restriction map for
pFN21AB4371
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# NO CUT ENZYMES ( 170 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AlwI AlwNI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AspI AvaI AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbvCI BbvI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmAI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HgaI Hin1I
HincII HindII HindIII HpaI KasI
KspI MaeI MaeII MaeIII MfeI
MflI MluI MluNI MroI MscI
MspA1I MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI TseI
Tsp45I Tsp509I Tth111I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 75 GGTACC G^GTACC
AfaI 76 GTAC GT^AC
AflIII 465 ACATGT A^C[GA][CT]GT
AluI 287 AGCT AG^CT
AocI 409 CCTCAGG CC^T[ACGT]AGG
Aor51HI 305 AGCGCT AGC^GCT
Asp718I 75 GGTACC G^GTACC
AspHI 199 GAGCAC G[AT]GC[AT]^C
AvaII 406 GGTCC G^G[AT]CC
BanI 75 GGTACC G^G[CT][GA]CC
BbsI 109 GAAGAC GAAGAC(2/6)
BcnI 312 CCGGG CC^[GC]GG
BpuAI 109 GAAGAC GAAGAC(2/6)
BsaBI 207 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 301 GAGGAG GAGGAG(10/8)
BsgI 220 GTGCAG GTGCAG(16/14)
BsiHKAI 199 GAGCAC G[AT]GC[AT]^C
BspLU11I 465 ACATGT A^CATGT
BstF5I 213 CATCC GGATG(2/0)
Bsu36I 409 CCTCAGG CC^T[ACGT]AGG
Cac8I 172 GCTGGC GC[ACGT]^[ACGT]GC
CfoI 306 GCGC GCG^C
DdeI 410 CTCAG C^T[ACGT]AG
EarI 34 CTCTTC CTCTTC(1/4)
Eco47III 305 AGCGCT AGC^GCT
Eco81I 409 CCTCAGG CC^T[ACGT]AGG
EcoNI 294 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Fnu4HI 421 GCGGC GC^[ACGT]GC
FokI 213 CATCC GGATG(9/13)
HaeII 305 AGCGCT [GA]GCGC^[CT]
HhaI 306 GCGC GCG^C
HinP1I 306 GCGC G^CGC
HinfI 22 GAGTC G^A[ACGT]TC
ItaI 421 GCGGC GC^[ACGT]GC
KpnI 75 GGTACC GGTAC^C
Ksp632I 34 CTCTTC CTCTTC(1/4)
MamI 207 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 213 CATCCGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 312 CCGGG CC^[GC]GG
NlaIII 466 CATG CATG^
NspI 465 ACATGT [GA]CATG^[CT]
PleI 22 GAGTC GAGTC(4/5)
PpuMI 405 AGGTCCT [GA]G^G[AT]CC[CT]
RsaI 76 GTAC GT^AC
SapI 33 GCTCTTC GCTCTTC(1/4)
SfcI 237 CTACAG C^T[GA][CT]AG
StuI 373 AGGCCT AGG^CCT
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
BmyI 199 GAGCAC G[AGT]GC[ACT]^C
367 GTGCCC
Bsp1286I 199 GAGCAC G[AGT]GC[ACT]^C
367 GTGCCC
BsrI 120 ACTGG ACTGG(1/-1)
357 ACTGG
DpnII 51 GATC ^GATC
207 GATC
DraII 324 GGGGCCT [GA]G^G[ACGT]CC[CT]
405 AGGTCCT
EcoO109I 324 GGGGCCT [GA]G^G[ACGT]CC[CT]
405 AGGTCCT
EcoT14I 71 CCAAGG C^C[AT][AT]GG
194 CCTTGG
FauI 156 CCCGC CCCGC(4/6)
322 GCGGG
HapII 232 CCGG C^CGG
312 CCGG
HpaII 232 CCGG C^CGG
312 CCGG
MboI 51 GATC ^GATC
207 GATC
MseI 59 TTAA T^TAA
134 TTAA
MspI 232 CCGG C^CGG
312 CCGG
NdeII 51 GATC ^GATC
207 GATC
PflMI 114 CCACACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
189 CCACCCCTTGG
Sau3AI 51 GATC ^GATC
207 GATC
StyI 71 CCAAGG C^C[AT][AT]GG
194 CCTTGG
TaqI 20 TCGA T^CGA
38 TCGA
Tru9I 59 TTAA T^TAA
134 TTAA
TspRI 119 CACTG CA[GC]TG[ACGT][ACGT]^
356 CACTG
TthHB8I 20 TCGA T^CGA
38 TCGA
Van91I 114 CCACACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
189 CCACCCCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AciI BpmI BsaJI BsiYI BslI
BsmFI BssKI BstNI Cfr13I EcoRII
HaeIII HphI MboII MnlI MvaI
MwoI NlaIV Sau96I ScrFI