Restriction map for pFN21AB4371
 (Circular) (Six-base) MAPSORT of: pFN21AB4371.seq  Check: 2550  from: 1  to: 5179



pFN21AB4371  5179 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:25  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2989     278 
   Size:         53      83     186     936    1453    2468

  Fragments arranged by size: 

                2468    1453     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2522    1937 
   Size:        585    4594
 
Acc65I G'GTAC_C

Cuts at:   2080    2500    2080 
   Size:        420    4759
 
AclI AA'CG_TT

Cuts at:   1563    3306    3679    1563 
   Size:       1743     373    3063

  Fragments arranged by size: 

                3063    1743     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3242    4626    4894    1632 
   Size:       1610    1384     268    1917

  Fragments arranged by size: 

                1917    1610    1384     268
 
AfeI AGC'GCT

Cuts at:   2312    2312 
   Size:       5179
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    4982
 
AflIII A'CryG_T

Cuts at:   1203    2470    4078    5055    1203 
   Size:       1267    1608     977    1327

  Fragments arranged by size: 

                1608    1327    1267     977
 
AgeI A'CCGG_T

Cuts at:   4010    4010 
   Size:       5179
 
AhdI GACnn_n'nnGTC

Cuts at:   3908    3908 
   Size:       5179
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5179
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5179
 
AlwNI CAG_nnn'CTG

Cuts at:   2922    4494    2922 
   Size:       1572    3607
 
ApaLI G'TGCA_C

Cuts at:   3236    4392    3236 
   Size:       1156    4023
 
ApoI r'AATT_y

Cuts at:   1493    2488    2714    2866    4975    1493 
   Size:        995     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     995     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3733     160 
   Size:        872    2701    1606

  Fragments arranged by size: 

                2701    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5179
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2504    1870 
   Size:         73     561    4545

  Fragments arranged by size: 

                4545     561      73
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   2064    2097    2064 
   Size:         33    5146
 
BamHI G'GATC_C

Cuts at:   2509    2876    2509 
   Size:        367    4812
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2080    2500    3955     618 
   Size:        325     217     627     293     420    1455    1842

  Fragments arranged by size: 

                1842    1455     627     420     325     293     217
 
BanII G_rGCy'C

Cuts at:   1391    2498    1391 
   Size:       1107    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2121     928 
   Size:        971     222    3986

  Fragments arranged by size: 

                3986     971     222
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3370    3404    1903 
   Size:         34    1433      34    3678

  Fragments arranged by size: 

                3678    1433      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3071    4281    3071 
   Size:       1210    3969
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5179
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3790     136 
   Size:        107     122      71     631     605    2118    1525

  Fragments arranged by size: 

                2118    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5174    5174 
   Size:       5179
 
BlpI GC'TnA_GC

Cuts at:   2596    2596 
   Size:       5179
 
Bme1580I G_kGCm'C

Cuts at:   1790    2376    3240    4396    1790 
   Size:        586     864    1156    2573

  Fragments arranged by size: 

                2573    1156     864     586
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5179
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2135    2910    3868 
   Size:        416      53      83     399      24     718     775     958

Cuts at:   3868     442 
   Size:       1753

  Fragments arranged by size: 

                1753     958     775     718     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5179
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2001    2324    2355    2448    3839    1239 
   Size:        762     323      31      93    1391    2579

  Fragments arranged by size: 

                2579    1391     762     323      93      31
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3304    4169    4467    3304 
   Size:        865     298    4016

  Fragments arranged by size: 

                4016     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3842     882 
   Size:        617    2343    2219

  Fragments arranged by size: 

                2343    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2970     493 
   Size:        711    1766    2702

  Fragments arranged by size: 

                2702    1766     711
 
BsaBI GATnn'nnATC

Cuts at:   2216    5173    2216 
   Size:       2957    2222
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2986    3368    4003     275 
   Size:         53      83     186     936    1453     382     635    1451

  Fragments arranged by size: 

                1453    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3612    4010    4284    4431    4842    3612 
   Size:        398     274     147     411    3949

  Fragments arranged by size: 

                3949     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    4954

  Fragments arranged by size: 

                4954     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2321    1186 
   Size:       1135    4044
 
BseYI C'CCAG_C

Cuts at:   4382    4878    4382 
   Size:        496    4683
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2246    1163 
   Size:        573     510    4096

  Fragments arranged by size: 

                4096     573     510
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2629    3390    4418    1540 
   Size:        275     186     628     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     628     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2208    3240    3325    4396    2208 
   Size:       1032      85    1071    2991

  Fragments arranged by size: 

                2991    1071    1032      85
 
BsmI GAATG_Cn'

Cuts at:   2690    2783    2690 
   Size:         93    5086
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2208    2376    2498    3240    3325    4396    1391 
   Size:        399     418     168     122     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     418     399     168     122      85
            
 
BspEI T'CCGG_A

Cuts at:   4842    4842 
   Size:       5179
 
BspHI T'CATG_A

Cuts at:   3068    4798    3068 
   Size:       1730    3449
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2534     844 
   Size:       1690    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3066    1394 
   Size:        429    1243    3507

  Fragments arranged by size: 

                3507    1243     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3674    3848      65 
   Size:       1183    2426     174    1396

  Fragments arranged by size: 

                2426    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3823    4010    1700 
   Size:        110    2013     187    2869

  Fragments arranged by size: 

                2869    2013     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5179
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5179
 
BssSI C'ACGA_G

Cuts at:   3239    4251    3239 
   Size:       1012    4167
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5179
 
BstBI TT'CG_AA

Cuts at:   3983    3983 
   Size:       5179
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5179
 
BstYI r'GATC_y

Cuts at:   1972    2509    2876    3261    3278    4730    4816    4828    5174 
   Size:        537     367     385      17    1452      86      12     346

Cuts at:   5174    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     537     385     367     346      86      17
                  12
 
Bsu36I CC'TnA_GG

Cuts at:   2415    2415 
   Size:       5179
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    4627
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4440

  Fragments arranged by size: 

                4440     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2695    3489    3509    2695 
   Size:        794      20    4365

  Fragments arranged by size: 

                4365     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5179
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5144
 
DraI TTT'AAA

Cuts at:   2483    2483 
   Size:       5179
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4186     809 
   Size:        507    2870    1802

  Fragments arranged by size: 

                2870    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2626    3515    5144 
   Size:         54    1425      50     275      18     796     889    1629

Cuts at:   5144       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425     889     796     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2626    1537 
   Size:        275     814    4090

  Fragments arranged by size: 

                4090     814     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2045    3109    1353 
   Size:        692    1064    3423

  Fragments arranged by size: 

                3423    1064     692
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3766    4140    4286     387 
   Size:       1064     138    2177     374     146    1280

  Fragments arranged by size: 

                2177    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2001    2324    2355    2448    3242    3839    4626 
   Size:        393     369     323      31      93     794     597     787

Cuts at:   4626    4894    1239 
   Size:        268    1524

  Fragments arranged by size: 

                1524     794     787     597     393     369     323     268
                  93      31
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2303    2303 
   Size:       5179
 
EcoO109I rG'GnC_Cy

Cuts at:   2330    2411    2330 
   Size:         81    5098
 
EcoRI G'AATT_C

Cuts at:   2488    2866    2488 
   Size:        378    4801
 
FspI TGC'GCA

Cuts at:   3685    3685 
   Size:       5179
 
HaeII r_GCGC'y

Cuts at:   1678    2314    4326    1678 
   Size:        636    2012    2531

  Fragments arranged by size: 

                2531    2012     636
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3820    3852    3894    3926    1373 
   Size:         32     135      32    2248      32      42      32    2626

  Fragments arranged by size: 

                2626    2248     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2523    2769     669 
   Size:        988     281     585     246    3079

  Fragments arranged by size: 

                3079     988     585     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5179
 
HpaI GTT'AAC

Cuts at:   2769    2769 
   Size:       5179
 
KpnI G_GTAC'C

Cuts at:   2084    2504    2084 
   Size:        420    4759
 
MfeI C'AATT_G

Cuts at:   2778    2778 
   Size:       5179
 
MluI A'CGCG_T

Cuts at:   5055    5055 
   Size:       5179
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4293    4477    1305 
   Size:       2988     184    2007

  Fragments arranged by size: 

                2988    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3357

  Fragments arranged by size: 

                3357    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2222    3137    3496    3655     518 
   Size:        648    1056     915     359     159    2042

  Fragments arranged by size: 

                2042    1056     915     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2595    3272    4420    4665    1606 
   Size:        989     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     989     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    4627
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5179
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5179
 
NotI GC'GGCC_GC

Cuts at:   2626    2626 
   Size:       5179
 
NspI r_CATG'y

Cuts at:   1132    1229    2474    2537    4082    1132 
   Size:         97    1245      63    1545    2229

  Fragments arranged by size: 

                2229    1545    1245      97      63
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5179
 
PciI A'CATG_T

Cuts at:   2470    4078    2470 
   Size:       1608    3571
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2125    2200    1108 
   Size:       1017      75    4087

  Fragments arranged by size: 

                4087    1017      75
 
PfoI T'CCnGG_A

Cuts at:   2464    2464 
   Size:       5179
 
PmeI GTTT'AAAC

Cuts at:   2483    2483 
   Size:       5179
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3248    3280    4761    4793    3248 
   Size:         32    1481      32    3634

  Fragments arranged by size: 

                3634    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2411    2411 
   Size:       5179
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5179
 
PsiI TTA'TAA

Cuts at:   2749    2749 
   Size:       5179
 
PstI C_TGCA'G

Cuts at:    830    2531     830 
   Size:       1701    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5179
 
SalI G'TCGA_C

Cuts at:   1936    2521    1936 
   Size:        585    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2045    2045 
   Size:       5179
 
SbfI CC_TGCA'GG

Cuts at:   2531    2531 
   Size:       5179
 
ScaI AGT'ACT

Cuts at:   3427    5139    3427 
   Size:       1712    3467
 
SfcI C'TryA_G

Cuts at:    826    1045    2242    2527    3662    4343    4534     826 
   Size:        219    1197     285    1135     681     191    1471

  Fragments arranged by size: 

                1471    1197    1135     681     285     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5179
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5179
 
SmaI CCC'GGG

Cuts at:   1872    2506    1872 
   Size:        634    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3283    4184    4446     820 
   Size:        197     926    1340     901     262    1553

  Fragments arranged by size: 

                1553    1340     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    2970     493 
   Size:       2477    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5179
 
SphI G_CATG'C

Cuts at:   1132    2537    1132 
   Size:       1405    3774
 
SspI AAT'ATT

Cuts at:      5      52    3103       5 
   Size:         47    3051    2081

  Fragments arranged by size: 

                3051    2081      47
 
StuI AGG'CCT

Cuts at:   1517    2380    1517 
   Size:        863    4316
 
StyI C'CwwG_G

Cuts at:    513    1065    2076    2199    2618     513 
   Size:        552    1011     123     419    3074

  Fragments arranged by size: 

                3074    1011     552     419     123
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3206    3223    3376    3561    4006     325 
   Size:       1255    1626      17     153     185     445    1498

  Fragments arranged by size: 

                1626    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3425    5137      96 
   Size:        274      80      33      51    1443    1448    1712     138

  Fragments arranged by size: 

                1712    1448    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5139    5171    5139 
   Size:         32    5147
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5179
 
XbaI T'CTAG_A

Cuts at:   2515    2515 
   Size:       5179
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5179
 
XmaI C'CCGG_G

Cuts at:   1870    2504    1870 
   Size:        634    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3308    3308 
   Size:       5179
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2987     276 
   Size:         53      83     186     936    1453    2468

  Fragments arranged by size: 

                2468    1453     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI     BaeI    BamHI     BanI    BanII     BbsI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  Eco57MI    EcoNI EcoO109I    EcoRI     FspI    HaeII    Hin4I
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NspI     PasI
     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI
     PstI     PvuI     SalI     SapI     SbfI     ScaI     SfcI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AloI     ApaI     AscI    AvrII     BbeI    BbvCI    BfrBI
     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I   DraIII  EcoICRI
    EcoRV     FalI     FseI    FspAI     KasI     NaeI     NarI   NgoMIV
     NruI     NsiI     PacI     PmlI   PspOMI    PspXI     PsrI    PvuII
    RsrII     SacI    SacII    SanDI    SexAI     SfiI     SfoI     SrfI
     SwaI     XcmI
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