(Circular) (Six-base) MAPSORT of: pFN21AB4325.seq Check: 5821 from: 1 to: 6013 pFN21AB4325 6013 bp With 182 enzymes: SgfI * October 20, 2009 10:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2615 2615 Size: 6013 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3823 278 Size: 53 83 186 936 2287 2468 Fragments arranged by size: 2468 2287 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2277 3356 1937 Size: 340 1079 4594 Fragments arranged by size: 4594 1079 340 Acc65I G'GTAC_C Cuts at: 3334 3334 Size: 6013 AclI AA'CG_TT Cuts at: 1563 4140 4513 1563 Size: 2577 373 3063 Fragments arranged by size: 3063 2577 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2268 2711 2942 3137 4076 5460 5728 1632 Size: 636 443 231 195 939 1384 268 1917 Fragments arranged by size: 1917 1384 939 636 443 268 231 195 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5816 AflIII A'CryG_T Cuts at: 1203 4912 5889 1203 Size: 3709 977 1327 Fragments arranged by size: 3709 1327 977 AgeI A'CCGG_T Cuts at: 4844 4844 Size: 6013 AhdI GACnn_n'nnGTC Cuts at: 4742 4742 Size: 6013 AleI CACnn'nnGTG Cuts at: 1166 2819 3128 1166 Size: 1653 309 4051 Fragments arranged by size: 4051 1653 309 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6013 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3287 3319 3287 Size: 32 5981 AlwNI CAG_nnn'CTG Cuts at: 2345 3160 3756 5328 2345 Size: 815 596 1572 3030 Fragments arranged by size: 3030 1572 815 596 ApaLI G'TGCA_C Cuts at: 4070 5226 4070 Size: 1156 4857 ApoI r'AATT_y Cuts at: 1493 2287 2902 2952 3322 3548 3700 5809 1493 Size: 794 615 50 370 226 152 2109 1697 Fragments arranged by size: 2109 1697 794 615 370 226 152 50 AseI AT'TA_AT Cuts at: 160 1032 4567 160 Size: 872 3535 1606 Fragments arranged by size: 3535 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6013 AvaI C'yCGr_G Cuts at: 1870 1943 3338 1870 Size: 73 1395 4545 Fragments arranged by size: 4545 1395 73 BamHI G'GATC_C Cuts at: 2709 3343 3710 2709 Size: 634 367 5012 Fragments arranged by size: 5012 634 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2549 2575 3254 3334 4789 Size: 325 217 627 762 26 679 80 1455 Cuts at: 4789 618 Size: 1842 Fragments arranged by size: 1842 1455 762 679 627 325 217 80 26 BanII G_rGCy'C Cuts at: 1391 2129 2246 3090 3332 1391 Size: 738 117 844 242 4072 Fragments arranged by size: 4072 844 738 242 117 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2953 928 Size: 971 1054 3988 Fragments arranged by size: 3988 1054 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2806 2840 4204 4238 1903 Size: 34 869 34 1364 34 3678 Fragments arranged by size: 3678 1364 869 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3905 5115 3905 Size: 1210 4803 BclI T'GATC_A Cuts at: 1548 1548 Size: 6013 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3182 4624 Size: 107 122 71 631 605 337 1173 1442 Cuts at: 4624 136 Size: 1525 Fragments arranged by size: 1525 1442 1173 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2746 6008 2746 Size: 3262 2751 BlpI GC'TnA_GC Cuts at: 3430 3430 Size: 6013 Bme1580I G_kGCm'C Cuts at: 1790 3259 4074 5230 1790 Size: 1469 815 1156 2573 Fragments arranged by size: 2573 1469 1156 815 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6013 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3744 4702 442 Size: 416 53 83 399 24 2327 958 1753 Fragments arranged by size: 2327 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6013 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4673 1239 Size: 3434 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2414 4138 5003 5301 2414 Size: 1724 865 298 3126 Fragments arranged by size: 3126 1724 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2981 4676 882 Size: 617 1482 1695 2219 Fragments arranged by size: 2219 1695 1482 617 BsaAI yAC'GTr Cuts at: 493 1204 3804 493 Size: 711 2600 2702 Fragments arranged by size: 2702 2600 711 BsaBI GATnn'nnATC Cuts at: 6007 6007 Size: 6013 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2582 2796 3820 4202 Size: 53 83 186 936 1049 214 1024 382 Cuts at: 4202 4837 275 Size: 635 1451 Fragments arranged by size: 1451 1049 1024 936 635 382 214 186 83 53 BsaWI w'CCGG_w Cuts at: 4446 4844 5118 5265 5676 4446 Size: 398 274 147 411 4783 Fragments arranged by size: 4783 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3096 3126 3287 3317 1178 Size: 30 165 30 1693 30 161 30 3874 Fragments arranged by size: 3874 1693 165 161 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3257 1186 Size: 2071 3942 BseYI C'CCAG_C Cuts at: 2523 2721 5216 5712 2523 Size: 198 2495 496 2824 Fragments arranged by size: 2824 2495 496 198 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2537 3209 1163 Size: 573 801 672 3967 Fragments arranged by size: 3967 801 672 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2655 3463 4224 5252 1540 Size: 275 186 654 808 761 1028 2301 Fragments arranged by size: 2301 1028 808 761 654 275 186 BsiHKAI G_wGCw'C Cuts at: 3090 4074 4159 5230 3090 Size: 984 85 1071 3873 Fragments arranged by size: 3873 1071 984 85 BsmI GAATG_Cn' Cuts at: 2382 3524 3617 2382 Size: 1142 93 4778 Fragments arranged by size: 4778 1142 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2129 2246 3090 3259 3332 4074 4159 Size: 399 339 117 844 169 73 742 85 Cuts at: 4159 5230 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 844 742 399 339 169 117 85 73 BspEI T'CCGG_A Cuts at: 5676 5676 Size: 6013 BspHI T'CATG_A Cuts at: 3198 3902 5632 3198 Size: 704 1730 3579 Fragments arranged by size: 3579 1730 704 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2615 3184 3301 3368 844 Size: 1771 569 117 67 3489 Fragments arranged by size: 3489 1771 569 117 67 BsrBI CCG'CTC Cuts at: 1394 1823 3900 1394 Size: 429 2077 3507 Fragments arranged by size: 3507 2077 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2442 4508 4682 65 Size: 1183 1194 2066 174 1396 Fragments arranged by size: 2066 1396 1194 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2578 3021 4657 4844 1700 Size: 110 768 443 1636 187 2869 Fragments arranged by size: 2869 1636 768 443 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6013 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6013 BssSI C'ACGA_G Cuts at: 4073 5085 4073 Size: 1012 5001 BstAPI GCAn_nnn'nTGC Cuts at: 794 2056 794 Size: 1262 4751 BstBI TT'CG_AA Cuts at: 4817 4817 Size: 6013 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6013 BstYI r'GATC_y Cuts at: 1972 2089 2335 2709 2746 3343 3710 4095 4112 Size: 117 246 374 37 597 367 385 17 Cuts at: 4112 5564 5650 5662 6008 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 597 385 374 367 346 246 117 86 37 17 12 Bsu36I CC'TnA_GG Cuts at: 2562 2562 Size: 6013 BtgI C'CryG_G Cuts at: 513 1065 2610 513 Size: 552 1545 3916 Fragments arranged by size: 3916 1545 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5274 Fragments arranged by size: 5274 683 56 BtsI GCAGTG_nn' Cuts at: 3529 4323 4343 3529 Size: 794 20 5199 Fragments arranged by size: 5199 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6013 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5978 DraI TTT'AAA Cuts at: 3317 3317 Size: 6013 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5020 809 Size: 507 3704 1802 Fragments arranged by size: 3704 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3023 3460 4349 Size: 54 1425 50 275 18 1193 437 889 Cuts at: 4349 5978 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1193 889 437 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3460 1537 Size: 275 1648 4090 Fragments arranged by size: 4090 1648 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2843 3943 1353 Size: 1490 1100 3423 Fragments arranged by size: 3423 1490 1100 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4600 4974 5120 387 Size: 1064 138 3011 374 146 1280 Fragments arranged by size: 3011 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3088 3088 Size: 6013 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2268 2711 2942 3137 4076 4673 5460 Size: 393 636 443 231 195 939 597 787 Cuts at: 5460 5728 1239 Size: 268 1524 Fragments arranged by size: 1524 939 787 636 597 443 393 268 231 195 EcoRI G'AATT_C Cuts at: 2952 3322 3700 2952 Size: 370 378 5265 Fragments arranged by size: 5265 378 370 FspI TGC'GCA Cuts at: 2381 4519 2381 Size: 2138 3875 HaeII r_GCGC'y Cuts at: 1678 5160 1678 Size: 3482 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2762 2794 2878 2910 3096 Size: 32 135 32 1190 32 84 32 186 Cuts at: 3096 3128 3209 3241 4654 4686 4728 4760 1373 Size: 32 81 32 1413 32 42 32 2626 Fragments arranged by size: 2626 1413 1190 186 135 84 81 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2410 2923 3357 3603 669 Size: 988 281 472 513 434 246 3079 Fragments arranged by size: 3079 988 513 472 434 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6013 HpaI GTT'AAC Cuts at: 2410 3603 2410 Size: 1193 4820 KpnI G_GTAC'C Cuts at: 3338 3338 Size: 6013 MfeI C'AATT_G Cuts at: 3612 3612 Size: 6013 MluI A'CGCG_T Cuts at: 5889 5889 Size: 6013 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2949 3078 5127 5311 1305 Size: 1644 129 2049 184 2007 Fragments arranged by size: 2049 2007 1644 184 129 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4191 Fragments arranged by size: 4191 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2766 2819 3128 3140 3971 4330 4489 Size: 648 1600 53 309 12 831 359 159 Cuts at: 4489 518 Size: 2042 Fragments arranged by size: 2042 1600 831 648 359 309 159 53 12 MspA1I CmG'CkG Cuts at: 1606 3279 3429 4106 5254 5499 1606 Size: 1673 150 677 1148 245 2120 Fragments arranged by size: 2120 1673 1148 677 245 150 NaeI GCC'GGC Cuts at: 2580 2580 Size: 6013 NcoI C'CATG_G Cuts at: 513 1065 2610 513 Size: 552 1545 3916 Fragments arranged by size: 3916 1545 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6013 NgoMIV G'CCGG_C Cuts at: 2578 2578 Size: 6013 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6013 NotI GC'GGCC_GC Cuts at: 3460 3460 Size: 6013 NspI r_CATG'y Cuts at: 1132 1229 2813 3371 4916 1132 Size: 97 1584 558 1545 2229 Fragments arranged by size: 2229 1584 1545 558 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6013 PciI A'CATG_T Cuts at: 4912 4912 Size: 6013 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6013 PmeI GTTT'AAAC Cuts at: 3317 3317 Size: 6013 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3287 3319 4082 4114 5595 5627 3287 Size: 32 763 32 1481 32 3673 Fragments arranged by size: 3673 1481 763 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6013 PsiI TTA'TAA Cuts at: 3583 3583 Size: 6013 PstI C_TGCA'G Cuts at: 830 2435 2595 3312 3365 830 Size: 1605 160 717 53 3478 Fragments arranged by size: 3478 1605 717 160 53 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6013 SacI G_AGCT'C Cuts at: 3090 3090 Size: 6013 SalI G'TCGA_C Cuts at: 1936 3355 1936 Size: 1419 4594 SapI GCTCTTCn'nnn_ Cuts at: 2843 2843 Size: 6013 SbfI CC_TGCA'GG Cuts at: 3312 3365 3312 Size: 53 5960 ScaI AGT'ACT Cuts at: 4261 5973 4261 Size: 1712 4301 SfcI C'TryA_G Cuts at: 826 1045 2431 2591 3308 3361 4496 5177 5368 Size: 219 1386 160 717 53 1135 681 191 Cuts at: 5368 826 Size: 1471 Fragments arranged by size: 1471 1386 1135 717 681 219 191 160 53 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6013 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6013 SmaI CCC'GGG Cuts at: 1872 3340 1872 Size: 1468 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2393 4117 5018 5280 820 Size: 197 926 450 1724 901 262 1553 Fragments arranged by size: 1724 1553 926 901 450 262 197 SnaBI TAC'GTA Cuts at: 493 3804 493 Size: 3311 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6013 SphI G_CATG'C Cuts at: 1132 2813 3371 1132 Size: 1681 558 3774 Fragments arranged by size: 3774 1681 558 SspI AAT'ATT Cuts at: 5 52 3937 5 Size: 47 3885 2081 Fragments arranged by size: 3885 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6013 StyI C'CwwG_G Cuts at: 513 1065 2610 2665 3452 513 Size: 552 1545 55 787 3074 Fragments arranged by size: 3074 1545 787 552 55 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4040 4057 4210 4395 4840 325 Size: 1255 2460 17 153 185 445 1498 Fragments arranged by size: 2460 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4259 5971 96 Size: 274 80 33 51 1443 2282 1712 138 Fragments arranged by size: 2282 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2559 2591 5973 6005 2559 Size: 32 3382 32 2567 Fragments arranged by size: 3382 2567 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2689 1316 Size: 1373 4640 XbaI T'CTAG_A Cuts at: 3349 3349 Size: 6013 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2617 2617 Size: 6013 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6013 XmaI C'CCGG_G Cuts at: 1870 3338 1870 Size: 1468 4545 XmnI GAAnn'nnTTC Cuts at: 2321 4142 2321 Size: 1821 4192 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3821 276 Size: 53 83 186 936 2287 2468 Fragments arranged by size: 2468 2287 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI