Restriction map for
pFN21AB4321
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# NO CUT ENZYMES ( 97 enzymes) #
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AatII AccII AclI AflII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaLI AscI AseI AsnI AspEI
AspI AviII BbeI BbrPI BbvCI
BcgI BfrI BglI BlpI Bpu1102I
BsiEI BsiWI BsmBI Bsp1407I BspDI
BsrBI BsrGI BssHII BssSI BstBI
BstUI Bsu36I CelII ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI EcoRV FseI FspI HpaI
KasI KspI MluI MspA1I MvnI
NarI NdeI NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI Sse8387I
SspBI SspI StuI SwaI Tth111I
XbaI XhoI
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# ONE CUT ENZYMES ( 40 enzymes) #
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Acc65I 1238 GGTACC G^GTACC
AccIII 149 TCCGGA T^CCGGA
ApaI 13 GGGCCC GGGCC^C
Asp700I 1126 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1238 GGTACC G^GTACC
AspHI 1622 GAGCAC G[AT]GC[AT]^C
AvrII 2166 CCTAGG C^CTAGG
BalI 161 TGGCCA TGG^CCA
BamHI 1974 GGATCC G^GATCC
BciVI 2971 GGATAC GTATCC(6/5)
BglII 2400 AGATCT A^GATCT
BlnI 2166 CCTAGG C^CTAGG
BsaBI 1975 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 149 TCCGGA [AT]^CCGG[AT]
BseAI 149 TCCGGA T^CCGGA
BsiHKAI 1622 GAGCAC G[AT]GC[AT]^C
BsmI 1086 GAATGC GAATGC(1/-1)
BspEI 149 TCCGGA T^CCGGA
BspHI 1277 TCATGA T^CATGA
BspLU11I 2792 ACATGT A^CATGT
BstAPI 1184 GCAAGAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2524 CCAATCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI 2702 CCATGG C^C[GA][CT]GG
HaeII 829 AGCGCC [GA]GCGC^[CT]
KpnI 1238 GGTACC GGTAC^C
MamI 1975 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 760 CAATTG C^AATTG
MluNI 161 TGGCCA TGG^CCA
MroI 149 TCCGGA T^CCGGA
MscI 161 TGGCCA TGG^CCA
MunI 760 CAATTG C^AATTG
NcoI 2702 CCATGG C^CATGG
PflMI 235 CCAAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1277 TCATGA T^CATGA
SgrAI 31 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 1347 CTTGAG C^T[CT][GA]AG
SnaBI 2810 TACGTA TAC^GTA
SpeI 1111 ACTAGT A^CTAGT
Van91I 235 CCAAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1126 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 682 GTATAC GT^[AC][GT]AC
2946 GTATAC
AcyI 29 GACGCC G[GA]^CG[CT]C
1774 GGCGTC
AflIII 1163 ACGTGT A^C[GA][CT]GT
2792 ACATGT
BclI 477 TGATCA T^GATCA
2196 TGATCA
BsaAI 1625 CACGTA [CT]AC^GT[GA]
2810 TACGTA
BsaHI 29 GACGCC G[GA]^CG[CT]C
1774 GGCGTC
BsaI 1534 GAGACC GGTCTC(1/5)
2631 GAGACC
BsgI 98 CTGCAC GTGCAG(16/14)
912 GTGCAG
BspMI 1204 GCAGGT ACCTGC(4/8)
2050 GCAGGT
Bst1107I 682 GTATAC GTA^TAC
2946 GTATAC
BstZ17I 682 GTATAC GTA^TAC
2946 GTATAC
EaeI 83 TGGCCG [CT]^GGCC[GA]
161 TGGCCA
FbaI 477 TGATCA T^GATCA
2196 TGATCA
Hin1I 29 GACGCC G[GA]^CG[CT]C
1774 GGCGTC
HincII 787 GTCAAC GT[CT]^[GA]AC
2788 GTCAAC
HindII 787 GTCAAC GT[CT]^[GA]AC
2788 GTCAAC
HindIII 543 AAGCTT A^AGCTT
992 AAGCTT
NaeI 32 GCCGGC GCC^GGC
85 GCCGGC
NgoMIV 32 GCCGGC G^CCGGC
85 GCCGGC
SmaI 17 CCCGGG CCC^GGG
2468 CCCGGG
XcmI 235 CCAAAAAATGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1846 CCAAGCCTAATATGG
XmaCI 17 CCCGGG C^CCGGG
2468 CCCGGG
XmaI 17 CCCGGG C^CCGGG
2468 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaI AvaII BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrDI BsrFI BsrI BssKI BstEII
BstF5I BstNI BstPI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoO65I EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NsiI NspI PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SphI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII