(Circular) (Six-base) MAPSORT of: pFN21AB4321.seq Check: 1193 from: 1 to: 7717 pFN21AB4321 7717 bp With 182 enzymes: SgfI * October 20, 2009 10:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3200 4046 3200 Size: 846 6871 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5527 278 Size: 53 83 186 936 3991 2468 Fragments arranged by size: 3991 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2688 4952 5060 1937 Size: 751 2264 108 4594 Fragments arranged by size: 4594 2264 751 108 Acc65I G'GTAC_C Cuts at: 3243 5038 3243 Size: 1795 5922 AclI AA'CG_TT Cuts at: 1563 5844 6217 1563 Size: 4281 373 3063 Fragments arranged by size: 4281 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3588 3640 4853 5780 7164 7432 1632 Size: 1956 52 1213 927 1384 268 1917 Fragments arranged by size: 1956 1917 1384 1213 927 268 52 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7520 AflIII A'CryG_T Cuts at: 1203 3168 4797 6616 7593 1203 Size: 1965 1629 1819 977 1327 Fragments arranged by size: 1965 1819 1629 1327 977 AgeI A'CCGG_T Cuts at: 6548 6548 Size: 7717 AhdI GACnn_n'nnGTC Cuts at: 6446 6446 Size: 7717 AleI CACnn'nnGTG Cuts at: 1166 3425 3686 1166 Size: 2259 261 5197 Fragments arranged by size: 5197 2259 261 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7717 AlwNI CAG_nnn'CTG Cuts at: 5460 7032 5460 Size: 1572 6145 ApaI G_GGCC'C Cuts at: 2022 2022 Size: 7717 ApaLI G'TGCA_C Cuts at: 5774 6930 5774 Size: 1156 6561 ApoI r'AATT_y Cuts at: 1493 2188 2396 3621 4001 4061 4524 5026 5252 Size: 695 208 1225 380 60 463 502 226 Cuts at: 5252 5404 7513 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1225 695 502 463 380 226 208 152 60 AseI AT'TA_AT Cuts at: 160 1032 6271 160 Size: 872 5239 1606 Fragments arranged by size: 5239 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7717 AvaI C'yCGr_G Cuts at: 1870 1943 2014 2022 4473 4493 5042 1870 Size: 73 71 8 2451 20 549 4545 Fragments arranged by size: 4545 2451 549 73 71 20 8 AvrII C'CTAG_G Cuts at: 4171 4171 Size: 7717 BamHI G'GATC_C Cuts at: 3979 5047 5414 3979 Size: 1068 367 6282 Fragments arranged by size: 6282 1068 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2854 2903 3243 5038 6493 Size: 325 217 627 1067 49 340 1795 1455 Cuts at: 6493 618 Size: 1842 Fragments arranged by size: 1842 1795 1455 1067 627 340 325 217 49 BanII G_rGCy'C Cuts at: 1391 2015 2022 2119 3359 3367 5036 1391 Size: 624 7 97 1240 8 1669 4072 Fragments arranged by size: 4072 1669 1240 624 97 8 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2722 3607 3646 4841 928 Size: 971 823 885 39 1195 3804 Fragments arranged by size: 3804 1195 971 885 823 39 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5908 5942 1903 Size: 34 50 34 3887 34 3678 Fragments arranged by size: 3887 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4970 5609 6819 4970 Size: 639 1210 5868 Fragments arranged by size: 5868 1210 639 BclI T'GATC_A Cuts at: 1548 2482 4201 1548 Size: 934 1719 5064 Fragments arranged by size: 5064 1719 934 BfrBI ATG'CAT Cuts at: 2871 3913 4850 2871 Size: 1042 937 5738 Fragments arranged by size: 5738 1042 937 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6328 136 Size: 107 122 71 631 605 4656 1525 Fragments arranged by size: 4656 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4405 7712 4405 Size: 3307 4410 BlpI GC'TnA_GC Cuts at: 5134 5134 Size: 7717 Bme1580I G_kGCm'C Cuts at: 1790 2022 2859 3930 5778 6934 1790 Size: 232 837 1071 1848 1156 2573 Fragments arranged by size: 2573 1848 1156 1071 837 232 BmgBI CAC'GTC Cuts at: 1318 2130 3169 1318 Size: 812 1039 5866 Fragments arranged by size: 5866 1039 812 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2444 3680 4654 Size: 416 53 83 399 24 1027 1236 974 Cuts at: 4654 5448 6406 442 Size: 794 958 1753 Fragments arranged by size: 1753 1236 1027 974 958 794 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7717 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4300 4300 Size: 7717 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2448 2769 3399 4612 4920 6377 1239 Size: 1209 321 630 1213 308 1457 2579 Fragments arranged by size: 2579 1457 1213 1209 630 321 308 Bpu10I CC'TnA_GC Cuts at: 4008 5001 4008 Size: 993 6724 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3373 5842 6707 7005 3373 Size: 2469 865 298 4085 Fragments arranged by size: 4085 2469 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3533 4630 6380 882 Size: 617 2034 1097 1750 2219 Fragments arranged by size: 2219 2034 1750 1097 617 BsaAI yAC'GTr Cuts at: 493 1204 3632 4817 5508 493 Size: 711 2428 1185 691 2702 Fragments arranged by size: 2702 2428 1185 711 691 BsaBI GATnn'nnATC Cuts at: 3984 7711 3984 Size: 3727 3990 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2035 3780 5524 5906 Size: 53 83 186 936 502 1745 1744 382 Cuts at: 5906 6541 275 Size: 635 1451 Fragments arranged by size: 1745 1744 1451 936 635 502 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2154 6150 6548 6822 6969 7380 2154 Size: 3996 398 274 147 411 2491 Fragments arranged by size: 3996 2491 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7492 Fragments arranged by size: 7492 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3075 3236 3776 1186 Size: 1889 161 540 5127 Fragments arranged by size: 5127 1889 540 161 BseYI C'CCAG_C Cuts at: 2077 2174 6920 7416 2077 Size: 97 4746 496 2378 Fragments arranged by size: 4746 2378 496 97 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2088 2938 1163 Size: 573 352 850 5942 Fragments arranged by size: 5942 850 573 352 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5167 5928 6956 1540 Size: 275 186 3166 761 1028 2301 Fragments arranged by size: 3166 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3631 5778 5863 6934 3631 Size: 2147 85 1071 4414 Fragments arranged by size: 4414 2147 1071 85 BsmI GAATG_Cn' Cuts at: 3097 5228 5321 3097 Size: 2131 93 5493 Fragments arranged by size: 5493 2131 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2015 2022 2119 2859 3359 3367 3631 Size: 399 225 7 97 740 500 8 264 Cuts at: 3631 3930 5036 5778 5863 6934 1391 Size: 299 1106 742 85 1071 2174 Fragments arranged by size: 2174 1106 1071 742 740 500 399 299 264 225 97 85 8 7 BspEI T'CCGG_A Cuts at: 2154 7380 2154 Size: 5226 2491 BspHI T'CATG_A Cuts at: 3282 5606 7336 3282 Size: 2324 1730 3663 Fragments arranged by size: 3663 2324 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3200 4046 5072 844 Size: 2356 846 1026 3489 Fragments arranged by size: 3489 2356 1026 846 BsrBI CCG'CTC Cuts at: 1394 1823 5604 1394 Size: 429 3781 3507 Fragments arranged by size: 3781 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2185 2532 2664 6212 6386 65 Size: 1183 937 347 132 3548 174 1396 Fragments arranged by size: 3548 1396 1183 937 347 174 132 BsrFI r'CCGG_y Cuts at: 1700 1810 2037 2090 4947 6361 6548 1700 Size: 110 227 53 2857 1414 187 2869 Fragments arranged by size: 2869 2857 1414 227 187 110 53 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7717 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7717 BssSI C'ACGA_G Cuts at: 5777 6789 5777 Size: 1012 6705 BstAPI GCAn_nnn'nTGC Cuts at: 794 3195 794 Size: 2401 5316 BstBI TT'CG_AA Cuts at: 6521 6521 Size: 7717 BstEII G'GTnAC_C Cuts at: 1184 2570 4234 5005 1184 Size: 1386 1664 771 3896 Fragments arranged by size: 3896 1664 1386 771 BstXI CCAn_nnnn'nTGG Cuts at: 4536 4536 Size: 7717 BstYI r'GATC_y Cuts at: 1972 2355 3292 3892 3979 4405 4895 5047 5414 Size: 383 937 600 87 426 490 152 367 Cuts at: 5414 5799 5816 7268 7354 7366 7712 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 937 600 490 426 385 383 367 346 152 87 86 17 12 BstZ17I GTA'TAC Cuts at: 2689 4953 2689 Size: 2264 5453 BtgI C'CryG_G Cuts at: 513 1065 4707 513 Size: 552 3642 3523 Fragments arranged by size: 3642 3523 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6978 Fragments arranged by size: 6978 683 56 BtsI GCAGTG_nn' Cuts at: 2827 3339 3774 5233 6027 6047 2827 Size: 512 435 1459 794 20 4497 Fragments arranged by size: 4497 1459 794 512 435 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7717 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7682 DraI TTT'AAA Cuts at: 5021 5021 Size: 7717 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6724 809 Size: 507 5408 1802 Fragments arranged by size: 5408 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2088 2166 5164 Size: 54 1425 50 275 18 258 78 2998 Cuts at: 5164 6053 7682 8 Size: 889 1629 43 Fragments arranged by size: 2998 1629 1425 889 275 258 78 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5164 1537 Size: 275 3352 4090 Fragments arranged by size: 4090 3352 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2250 2886 3894 5647 1353 Size: 897 636 1008 1753 3423 Fragments arranged by size: 3423 1753 1008 897 636 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3129 6304 6678 6824 387 Size: 1064 138 1540 3175 374 146 1280 Fragments arranged by size: 3175 1540 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2448 2769 3399 3588 3640 4612 4853 Size: 393 816 321 630 189 52 972 241 Cuts at: 4853 4920 5780 6377 7164 7432 1239 Size: 67 860 597 787 268 1524 Fragments arranged by size: 1524 972 860 816 787 630 597 393 321 268 241 189 67 52 EcoO109I rG'GnC_Cy Cuts at: 2018 2019 2218 2439 2612 2018 Size: 1 199 221 173 7123 Fragments arranged by size: 7123 221 199 173 1 EcoRI G'AATT_C Cuts at: 5026 5404 5026 Size: 378 7339 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2470 2470 Size: 7717 FspI TGC'GCA Cuts at: 6223 6223 Size: 7717 HaeII r_GCGC'y Cuts at: 1678 2838 6864 1678 Size: 1160 4026 2531 Fragments arranged by size: 4026 2531 1160 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3277 3309 4642 4674 6358 Size: 32 135 32 1705 32 1333 32 1684 Cuts at: 6358 6390 6432 6464 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1705 1684 1333 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2794 4795 5061 5307 669 Size: 988 281 856 2001 266 246 3079 Fragments arranged by size: 3079 2001 988 856 281 266 246 HindIII A'AGCT_T Cuts at: 748 2548 2997 748 Size: 1800 449 5468 Fragments arranged by size: 5468 1800 449 HpaI GTT'AAC Cuts at: 5307 5307 Size: 7717 KpnI G_GTAC'C Cuts at: 3247 5042 3247 Size: 1795 5922 MfeI C'AATT_G Cuts at: 2765 5316 2765 Size: 2551 5166 MluI A'CGCG_T Cuts at: 7593 7593 Size: 7717 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2333 3372 4782 6831 7015 1305 Size: 1028 1039 1410 2049 184 2007 Fragments arranged by size: 2049 2007 1410 1039 1028 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2168 10 Size: 54 1425 343 336 5559 Fragments arranged by size: 5559 1425 343 336 54 MslI CAynn'nnrTG Cuts at: 518 1166 2210 3210 3287 3425 3686 3972 5675 Size: 648 1044 1000 77 138 261 286 1703 Cuts at: 5675 6034 6193 518 Size: 359 159 2042 Fragments arranged by size: 2042 1703 1044 1000 648 359 286 261 159 138 77 MspA1I CmG'CkG Cuts at: 1606 5133 5810 6958 7203 1606 Size: 3527 677 1148 245 2120 Fragments arranged by size: 3527 2120 1148 677 245 NaeI GCC'GGC Cuts at: 2039 2092 2039 Size: 53 7664 NcoI C'CATG_G Cuts at: 513 1065 4707 513 Size: 552 3642 3523 Fragments arranged by size: 3642 3523 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7717 NgoMIV G'CCGG_C Cuts at: 2037 2090 2037 Size: 53 7664 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7717 NotI GC'GGCC_GC Cuts at: 5164 5164 Size: 7717 NsiI A_TGCA'T Cuts at: 2873 3915 4852 2873 Size: 1042 937 5738 Fragments arranged by size: 5738 1042 937 NspI r_CATG'y Cuts at: 1132 1229 2875 3066 3189 4348 4801 5075 6620 Size: 97 1646 191 123 1159 453 274 1545 Cuts at: 6620 1132 Size: 2229 Fragments arranged by size: 2229 1646 1545 1159 453 274 191 123 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7717 PciI A'CATG_T Cuts at: 4797 6616 4797 Size: 1819 5898 PflMI CCAn_nnn'nTGG Cuts at: 1108 2246 1108 Size: 1138 6579 PfoI T'CCnGG_A Cuts at: 2425 2425 Size: 7717 PmeI GTTT'AAAC Cuts at: 5021 5021 Size: 7717 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5786 5818 7299 7331 5786 Size: 32 1481 32 6172 Fragments arranged by size: 6172 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2218 2439 2612 2218 Size: 221 173 7323 Fragments arranged by size: 7323 221 173 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7717 PsiI TTA'TAA Cuts at: 5287 5287 Size: 7717 PspOMI G'GGCC_C Cuts at: 2018 2018 Size: 7717 PstI C_TGCA'G Cuts at: 830 2834 3586 4366 4859 5069 830 Size: 2004 752 780 493 210 3478 Fragments arranged by size: 3478 2004 780 752 493 210 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7717 SalI G'TCGA_C Cuts at: 1936 5059 1936 Size: 3123 4594 SbfI CC_TGCA'GG Cuts at: 5069 5069 Size: 7717 ScaI AGT'ACT Cuts at: 5965 7677 5965 Size: 1712 6005 SfcI C'TryA_G Cuts at: 826 1045 2830 3582 3874 4362 4855 4906 5065 Size: 219 1785 752 292 488 493 51 159 Cuts at: 5065 6200 6881 7072 826 Size: 1135 681 191 1471 Fragments arranged by size: 1785 1471 1135 752 681 493 488 292 219 191 159 51 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7717 SgrAI Cr'CCGG_yG Cuts at: 1700 2037 1700 Size: 337 7380 SmaI CCC'GGG Cuts at: 1872 2024 4475 5044 1872 Size: 152 2451 569 4545 Fragments arranged by size: 4545 2451 569 152 SmlI C'TyrA_G Cuts at: 820 1017 1943 3352 5821 6722 6984 820 Size: 197 926 1409 2469 901 262 1553 Fragments arranged by size: 2469 1553 1409 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4817 5508 493 Size: 4324 691 2702 Fragments arranged by size: 4324 2702 691 SpeI A'CTAG_T Cuts at: 152 3116 152 Size: 2964 4753 SphI G_CATG'C Cuts at: 1132 3066 3189 4348 5075 1132 Size: 1934 123 1159 727 3774 Fragments arranged by size: 3774 1934 1159 727 123 SspI AAT'ATT Cuts at: 5 52 5641 5 Size: 47 5589 2081 Fragments arranged by size: 5589 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7717 StyI C'CwwG_G Cuts at: 513 1065 4171 4707 5010 5156 513 Size: 552 3106 536 303 146 3074 Fragments arranged by size: 3106 3074 552 536 303 146 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2238 2822 3067 5744 5761 5914 6099 Size: 1255 658 584 245 2677 17 153 185 Cuts at: 6099 6544 325 Size: 445 1498 Fragments arranged by size: 2677 1498 1255 658 584 445 245 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5963 7675 96 Size: 274 80 33 51 1443 3986 1712 138 Fragments arranged by size: 3986 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2934 2966 7677 7709 2934 Size: 32 4711 32 2942 Fragments arranged by size: 4711 2942 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7717 XbaI T'CTAG_A Cuts at: 5053 5053 Size: 7717 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2247 3858 2247 Size: 1611 6106 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7717 XmaI C'CCGG_G Cuts at: 1870 2022 4473 5042 1870 Size: 152 2451 569 4545 Fragments arranged by size: 4545 2451 569 152 XmnI GAAnn'nnTTC Cuts at: 3135 5846 3135 Size: 2711 5006 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5525 276 Size: 53 83 186 936 3991 2468 Fragments arranged by size: 3991 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbeI BbvCI BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI