Restriction map for
pFN21AB4321
(Linear) (Six-base) MAPSORT of: KIBB4321.seq Check: 5257 from: 1 to: 3012
KIBB4321.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1195 2041 3012
Size: 1195 846 971
Fragments arranged by size:
1195 971 846
AccI GT'mk_AC
Cuts at: 0 683 2947 3012
Size: 683 2264 65
Fragments arranged by size:
2264 683 65
Acc65I G'GTAC_C
Cuts at: 0 1238 3012
Size: 1238 1774
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1583 1635 2848 3012
Size: 1583 52 1213 164
Fragments arranged by size:
1583 1213 164 52
AflIII A'CryG_T
Cuts at: 0 1163 2792 3012
Size: 1163 1629 220
Fragments arranged by size:
1629 1163 220
AleI CACnn'nnGTG
Cuts at: 0 1420 1681 3012
Size: 1420 261 1331
Fragments arranged by size:
1420 1331 261
ApaI G_GGCC'C
Cuts at: 0 17 3012
Size: 17 2995
ApoI r'AATT_y
Cuts at: 0 183 391 1616 1996 2056 2519 3012
Size: 183 208 1225 380 60 463 493
Fragments arranged by size:
1225 493 463 380 208 183 60
AvaI C'yCGr_G
Cuts at: 0 9 17 2468 2488 3012
Size: 9 8 2451 20 524
Fragments arranged by size:
2451 524 20 9 8
AvrII C'CTAG_G
Cuts at: 0 2166 3012
Size: 2166 846
BamHI G'GATC_C
Cuts at: 0 1974 3012
Size: 1974 1038
BanI G'GyrC_C
Cuts at: 0 849 898 1238 3012
Size: 849 49 340 1774
Fragments arranged by size:
1774 849 340 49
BanII G_rGCy'C
Cuts at: 0 10 17 114 1354 1362 3012
Size: 10 7 97 1240 8 1650
Fragments arranged by size:
1650 1240 97 10 8 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 717 1602 1641 2836 3012
Size: 717 885 39 1195 176
Fragments arranged by size:
1195 885 717 176 39
BciVI GTATCCnnnnn_n'
Cuts at: 0 2965 3012
Size: 2965 47
BclI T'GATC_A
Cuts at: 0 477 2196 3012
Size: 477 1719 816
Fragments arranged by size:
1719 816 477
BfrBI ATG'CAT
Cuts at: 0 866 1908 2845 3012
Size: 866 1042 937 167
Fragments arranged by size:
1042 937 866 167
BglII A'GATC_T
Cuts at: 0 2400 3012
Size: 2400 612
Bme1580I G_kGCm'C
Cuts at: 0 17 854 1925 3012
Size: 17 837 1071 1087
Fragments arranged by size:
1087 1071 837 17
BmgBI CAC'GTC
Cuts at: 0 125 1164 3012
Size: 125 1039 1848
Fragments arranged by size:
1848 1039 125
BmrI ACTGGGnnnn_n'
Cuts at: 0 439 1675 2649 3012
Size: 439 1236 974 363
Fragments arranged by size:
1236 974 439 363
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2295 3012
Size: 2295 717
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 443 764 1394 2607 2915 3012
Size: 443 321 630 1213 308 97
Fragments arranged by size:
1213 630 443 321 308 97
Bpu10I CC'TnA_GC
Cuts at: 0 2003 2996 3012
Size: 2003 993 16
Fragments arranged by size:
2003 993 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1368 3012
Size: 1368 1644
BsaI GGTCTCn'nnnn_
Cuts at: 0 1528 2625 3012
Size: 1528 1097 387
Fragments arranged by size:
1528 1097 387
BsaAI yAC'GTr
Cuts at: 0 1627 2812 3012
Size: 1627 1185 200
Fragments arranged by size:
1627 1185 200
BsaBI GATnn'nnATC
Cuts at: 0 1979 3012
Size: 1979 1033
BsaHI Gr'CG_yC
Cuts at: 0 30 1775 3012
Size: 30 1745 1237
Fragments arranged by size:
1745 1237 30
BsaWI w'CCGG_w
Cuts at: 0 149 3012
Size: 149 2863
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1070 1231 1771 3012
Size: 1070 161 540 1241
Fragments arranged by size:
1241 1070 540 161
BseYI C'CCAG_C
Cuts at: 0 72 169 3012
Size: 72 97 2843
Fragments arranged by size:
2843 97 72
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 933 3012
Size: 83 850 2079
Fragments arranged by size:
2079 850 83
BsiHKAI G_wGCw'C
Cuts at: 0 1626 3012
Size: 1626 1386
BsmI GAATG_Cn'
Cuts at: 0 1092 3012
Size: 1092 1920
Bsp1286I G_dGCh'C
Cuts at: 0 10 17 114 854 1354 1362 1626 1925
Size: 10 7 97 740 500 8 264 299
Cuts at: 1925 3012
Size: 1087
Fragments arranged by size:
1087 740 500 299 264 97 10 8
7
BspEI T'CCGG_A
Cuts at: 0 149 3012
Size: 149 2863
BspHI T'CATG_A
Cuts at: 0 1277 3012
Size: 1277 1735
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1195 2041 3012
Size: 1195 846 971
Fragments arranged by size:
1195 971 846
BsrDI GCAATG_nn'
Cuts at: 0 180 527 659 3012
Size: 180 347 132 2353
Fragments arranged by size:
2353 347 180 132
BsrFI r'CCGG_y
Cuts at: 0 32 85 2942 3012
Size: 32 53 2857 70
Fragments arranged by size:
2857 70 53 32
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1190 3012
Size: 1190 1822
BstEII G'GTnAC_C
Cuts at: 0 565 2229 3000 3012
Size: 565 1664 771 12
Fragments arranged by size:
1664 771 565 12
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2531 3012
Size: 2531 481
BstYI r'GATC_y
Cuts at: 0 350 1287 1887 1974 2400 2890 3012
Size: 350 937 600 87 426 490 122
Fragments arranged by size:
937 600 490 426 350 122 87
BstZ17I GTA'TAC
Cuts at: 0 684 2948 3012
Size: 684 2264 64
Fragments arranged by size:
2264 684 64
BtgI C'CryG_G
Cuts at: 0 2702 3012
Size: 2702 310
BtsI GCAGTG_nn'
Cuts at: 0 822 1334 1769 3012
Size: 822 512 435 1243
Fragments arranged by size:
1243 822 512 435
EaeI y'GGCC_r
Cuts at: 0 83 161 3012
Size: 83 78 2851
Fragments arranged by size:
2851 83 78
EarI CTCTTCn'nnn_
Cuts at: 0 245 881 1889 3012
Size: 245 636 1008 1123
Fragments arranged by size:
1123 1008 636 245
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1124 3012
Size: 1124 1888
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 443 764 1394 1583 1635 2607 2848 2915
Size: 443 321 630 189 52 972 241 67
Cuts at: 2915 3012
Size: 97
Fragments arranged by size:
972 630 443 321 241 189 97 67
52
EcoO109I rG'GnC_Cy
Cuts at: 0 13 14 213 434 607 3012
Size: 13 1 199 221 173 2405
Fragments arranged by size:
2405 221 199 173 13 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 465 3012
Size: 465 2547
HaeII r_GCGC'y
Cuts at: 0 833 3012
Size: 833 2179
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1272 1304 2637 2669 3012
Size: 1272 32 1333 32 343
Fragments arranged by size:
1333 1272 343 32 32
HincII GTy'rAC
Cuts at: 0 789 2790 3012
Size: 789 2001 222
Fragments arranged by size:
2001 789 222
HindIII A'AGCT_T
Cuts at: 0 543 992 3012
Size: 543 449 2020
Fragments arranged by size:
2020 543 449
KpnI G_GTAC'C
Cuts at: 0 1242 3012
Size: 1242 1770
MfeI C'AATT_G
Cuts at: 0 760 3012
Size: 760 2252
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 1367 2777 3012
Size: 328 1039 1410 235
Fragments arranged by size:
1410 1039 328 235
MscI TGG'CCA
Cuts at: 0 163 3012
Size: 163 2849
MslI CAynn'nnrTG
Cuts at: 0 205 1205 1282 1420 1681 1967 3012
Size: 205 1000 77 138 261 286 1045
Fragments arranged by size:
1045 1000 286 261 205 138 77
NaeI GCC'GGC
Cuts at: 0 34 87 3012
Size: 34 53 2925
Fragments arranged by size:
2925 53 34
NcoI C'CATG_G
Cuts at: 0 2702 3012
Size: 2702 310
NgoMIV G'CCGG_C
Cuts at: 0 32 85 3012
Size: 32 53 2927
Fragments arranged by size:
2927 53 32
NsiI A_TGCA'T
Cuts at: 0 868 1910 2847 3012
Size: 868 1042 937 165
Fragments arranged by size:
1042 937 868 165
NspI r_CATG'y
Cuts at: 0 870 1061 1184 2343 2796 3012
Size: 870 191 123 1159 453 216
Fragments arranged by size:
1159 870 453 216 191 123
PciI A'CATG_T
Cuts at: 0 2792 3012
Size: 2792 220
PflMI CCAn_nnn'nTGG
Cuts at: 0 241 3012
Size: 241 2771
PfoI T'CCnGG_A
Cuts at: 0 420 3012
Size: 420 2592
PpuMI rG'GwC_Cy
Cuts at: 0 213 434 607 3012
Size: 213 221 173 2405
Fragments arranged by size:
2405 221 213 173
PspOMI G'GGCC_C
Cuts at: 0 13 3012
Size: 13 2999
PstI C_TGCA'G
Cuts at: 0 829 1581 2361 2854 3012
Size: 829 752 780 493 158
Fragments arranged by size:
829 780 752 493 158
SfcI C'TryA_G
Cuts at: 0 825 1577 1869 2357 2850 2901 3012
Size: 825 752 292 488 493 51 111
Fragments arranged by size:
825 752 493 488 292 111 51
SgrAI Cr'CCGG_yG
Cuts at: 0 32 3012
Size: 32 2980
SmaI CCC'GGG
Cuts at: 0 19 2470 3012
Size: 19 2451 542
Fragments arranged by size:
2451 542 19
SmlI C'TyrA_G
Cuts at: 0 1347 3012
Size: 1347 1665
SnaBI TAC'GTA
Cuts at: 0 2812 3012
Size: 2812 200
SpeI A'CTAG_T
Cuts at: 0 1111 3012
Size: 1111 1901
SphI G_CATG'C
Cuts at: 0 1061 1184 2343 3012
Size: 1061 123 1159 669
Fragments arranged by size:
1159 1061 669 123
StyI C'CwwG_G
Cuts at: 0 2166 2702 3005 3012
Size: 2166 536 303 7
Fragments arranged by size:
2166 536 303 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 233 817 1062 3012
Size: 233 584 245 1950
Fragments arranged by size:
1950 584 245 233
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 929 961 3012
Size: 929 32 2051
Fragments arranged by size:
2051 929 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 242 1853 3012
Size: 242 1611 1159
Fragments arranged by size:
1611 1159 242
XmaI C'CCGG_G
Cuts at: 0 17 2468 3012
Size: 17 2451 544
Fragments arranged by size:
2451 544 17
XmnI GAAnn'nnTTC
Cuts at: 0 1130 3012
Size: 1130 1882
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AleI ApaI ApoI
AvaI AvrII BamHI BanI BanII BbsI BciVI BclI
BfrBI BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrDI
BsrFI BstAPI BstEII BstXI BstYI BstZ17I BtgI BtsI
EaeI EarI EciI Eco57MI EcoO109I FalI HaeII Hin4I
HincII HindIII KpnI MfeI MmeI MscI MslI NaeI
NcoI NgoMIV NsiI NspI PciI PflMI PfoI PpuMI
PspOMI PstI SfcI SgrAI SmaI SmlI SnaBI SpeI
SphI StyI TaqII TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AhdI AlfI AloI
AlwNI ApaLI AscI AseI AsiSI BaeI BbeI BbvCI
BcgI BglI BlpI BmtI BsaXI BsiEI BsiWI BsmBI
BsrBI BsrGI BssHII BssSI BstBI Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EagI EcoICRI EcoNI EcoRI
EcoRV FseI FspI FspAI HpaI KasI MluI MspA1I
NarI NdeI NheI NotI NruI PacI PasI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SrfI SspI StuI SwaI
TatI Tth111I XbaI XhoI ZraI