(Circular) (Six-base) MAPSORT of: pFN21AB4218.seq Check: 4906 from: 1 to: 7099 pFN21AB4218 7099 bp With 182 enzymes: SgfI * October 20, 2009 10:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3504 3504 Size: 7099 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3336 4909 278 Size: 53 83 186 936 1800 1573 2468 Fragments arranged by size: 2468 1800 1573 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4068 4442 1937 Size: 2131 374 4594 Fragments arranged by size: 4594 2131 374 Acc65I G'GTAC_C Cuts at: 2438 4420 2438 Size: 1982 5117 AclI AA'CG_TT Cuts at: 1563 5226 5599 1563 Size: 3663 373 3063 Fragments arranged by size: 3663 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4325 5162 6546 6814 1632 Size: 2693 837 1384 268 1917 Fragments arranged by size: 2693 1917 1384 837 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6902 AflIII A'CryG_T Cuts at: 1203 5998 6975 1203 Size: 4795 977 1327 Fragments arranged by size: 4795 1327 977 AgeI A'CCGG_T Cuts at: 5930 5930 Size: 7099 AhdI GACnn_n'nnGTC Cuts at: 3613 5828 3613 Size: 2215 4884 AleI CACnn'nnGTG Cuts at: 1166 2645 1166 Size: 1479 5620 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7099 AlwNI CAG_nnn'CTG Cuts at: 2087 4842 6414 2087 Size: 2755 1572 2772 Fragments arranged by size: 2772 2755 1572 ApaLI G'TGCA_C Cuts at: 5156 6312 5156 Size: 1156 5943 ApoI r'AATT_y Cuts at: 1493 2251 2547 3069 4298 4408 4634 4786 6895 Size: 758 296 522 1229 110 226 152 2109 Cuts at: 6895 1493 Size: 1697 Fragments arranged by size: 2109 1697 1229 758 522 296 226 152 110 AseI AT'TA_AT Cuts at: 160 1032 2886 5653 160 Size: 872 1854 2767 1606 Fragments arranged by size: 2767 1854 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7099 AvaI C'yCGr_G Cuts at: 1870 1943 2076 2196 2759 3735 3790 3839 4187 Size: 73 133 120 563 976 55 49 348 Cuts at: 4187 4424 1870 Size: 237 4545 Fragments arranged by size: 4545 976 563 348 237 133 120 73 55 49 AvrII C'CTAG_G Cuts at: 2158 2158 Size: 7099 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2430 2463 2430 Size: 33 7066 BamHI G'GATC_C Cuts at: 2657 4429 4796 2657 Size: 1772 367 4960 Fragments arranged by size: 4960 1772 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2438 3446 3492 3599 3730 Size: 325 217 627 651 1008 46 107 131 Cuts at: 3730 4183 4226 4420 5875 618 Size: 453 43 194 1455 1842 Fragments arranged by size: 1842 1455 1008 651 627 453 325 217 194 131 107 46 43 BanII G_rGCy'C Cuts at: 1391 2307 3840 4418 1391 Size: 916 1533 578 4072 Fragments arranged by size: 4072 1533 916 578 BbeI G_GCGC'C Cuts at: 3603 3603 Size: 7099 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2175 928 Size: 971 276 5852 Fragments arranged by size: 5852 971 276 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4071 4105 5290 5324 1903 Size: 34 2134 34 1185 34 3678 Fragments arranged by size: 3678 2134 1185 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3122 4991 6201 3122 Size: 1869 1210 4020 Fragments arranged by size: 4020 1869 1210 BclI T'GATC_A Cuts at: 1548 2386 1548 Size: 838 6261 BfrBI ATG'CAT Cuts at: 2855 2855 Size: 7099 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5710 136 Size: 107 122 71 631 605 4038 1525 Fragments arranged by size: 4038 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7094 7094 Size: 7099 BlpI GC'TnA_GC Cuts at: 3893 4516 3893 Size: 623 6476 Bme1580I G_kGCm'C Cuts at: 1790 2023 3735 4188 5160 6316 1790 Size: 233 1712 453 972 1156 2573 Fragments arranged by size: 2573 1712 1156 972 453 233 BmgBI CAC'GTC Cuts at: 1318 4219 1318 Size: 2901 4198 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2256 4830 5788 Size: 416 53 83 399 24 839 2574 958 Cuts at: 5788 442 Size: 1753 Fragments arranged by size: 2574 1753 958 839 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7099 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2039 3100 2039 Size: 1061 6038 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3150 5759 1239 Size: 1911 2609 2579 Fragments arranged by size: 2609 2579 1911 Bpu10I CC'TnA_GC Cuts at: 2802 2802 Size: 7099 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2460 3809 5224 6089 6387 2460 Size: 1349 1415 865 298 3172 Fragments arranged by size: 3172 1415 1349 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3083 3520 5762 882 Size: 617 1584 437 2242 2219 Fragments arranged by size: 2242 2219 1584 617 437 BsaAI yAC'GTr Cuts at: 493 1204 2189 3982 4890 493 Size: 711 985 1793 908 2702 Fragments arranged by size: 2702 1793 985 908 711 BsaBI GATnn'nnATC Cuts at: 7093 7093 Size: 7099 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3333 3600 3663 4906 Size: 53 83 186 936 1800 267 63 1243 Cuts at: 4906 5288 5923 275 Size: 382 635 1451 Fragments arranged by size: 1800 1451 1243 936 635 382 267 186 83 63 53 BsaWI w'CCGG_w Cuts at: 5532 5930 6204 6351 6762 5532 Size: 398 274 147 411 5869 Fragments arranged by size: 5869 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2165 2195 3572 3602 3986 Size: 30 165 30 762 30 1377 30 384 Cuts at: 3986 4016 1178 Size: 30 4261 Fragments arranged by size: 4261 1377 762 384 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2189 3945 4165 1186 Size: 1003 1756 220 4120 Fragments arranged by size: 4120 1756 1003 220 BseYI C'CCAG_C Cuts at: 2065 2164 4005 6302 6798 2065 Size: 99 1841 2297 496 2366 Fragments arranged by size: 2366 2297 1841 496 99 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2079 3392 3703 3962 4165 1163 Size: 573 343 1313 311 259 203 4097 Fragments arranged by size: 4097 1313 573 343 311 259 203 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3744 4549 5310 6338 1540 Size: 275 186 1743 805 761 1028 2301 Fragments arranged by size: 2301 1743 1028 805 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2476 3987 4122 5160 5245 6316 2476 Size: 1511 135 1038 85 1071 3259 Fragments arranged by size: 3259 1511 1071 1038 135 85 BsmI GAATG_Cn' Cuts at: 3085 4610 4703 3085 Size: 1525 93 5481 Fragments arranged by size: 5481 1525 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2274 2274 Size: 7099 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2023 2307 2476 3735 3840 3987 4122 Size: 399 233 284 169 1259 105 147 135 Cuts at: 4122 4188 4418 5160 5245 6316 1391 Size: 66 230 742 85 1071 2174 Fragments arranged by size: 2174 1259 1071 742 399 284 233 230 169 147 135 105 85 66 BspEI T'CCGG_A Cuts at: 6762 6762 Size: 7099 BspHI T'CATG_A Cuts at: 4333 4988 6718 4333 Size: 655 1730 4714 Fragments arranged by size: 4714 1730 655 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2072 3504 4023 4454 844 Size: 1228 1432 519 431 3489 Fragments arranged by size: 3489 1432 1228 519 431 BsrBI CCG'CTC Cuts at: 1394 1823 4021 4224 4986 1394 Size: 429 2198 203 762 3507 Fragments arranged by size: 3507 2198 762 429 203 BsrDI GCAATG_nn' Cuts at: 65 1248 5594 5768 65 Size: 1183 4346 174 1396 Fragments arranged by size: 4346 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3489 5743 5930 1700 Size: 110 1679 2254 187 2869 Fragments arranged by size: 2869 2254 1679 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7099 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7099 BssSI C'ACGA_G Cuts at: 5159 6171 5159 Size: 1012 6087 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7099 BstBI TT'CG_AA Cuts at: 4372 5903 4372 Size: 1531 5568 BstEII G'GTnAC_C Cuts at: 1184 3760 1184 Size: 2576 4523 BstXI CCAn_nnnn'nTGG Cuts at: 3128 3772 3128 Size: 644 6455 BstYI r'GATC_y Cuts at: 1972 2657 2806 4429 4796 5181 5198 6650 6736 Size: 685 149 1623 367 385 17 1452 86 Cuts at: 6736 6748 7094 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1623 1452 685 385 367 346 149 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3551 4026 3551 Size: 475 6624 BtgI C'CryG_G Cuts at: 513 1065 2004 3610 3831 513 Size: 552 939 1606 221 3781 Fragments arranged by size: 3781 1606 939 552 221 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6360 Fragments arranged by size: 6360 683 56 BtsI GCAGTG_nn' Cuts at: 2652 4615 5409 5429 2652 Size: 1963 794 20 4322 Fragments arranged by size: 4322 1963 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7099 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7064 DraI TTT'AAA Cuts at: 4403 4403 Size: 7099 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3052 6106 809 Size: 507 1736 3054 1802 Fragments arranged by size: 3054 1802 1736 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3535 3741 3909 Size: 54 1425 50 275 18 1705 206 168 Cuts at: 3909 3963 4546 5435 7064 8 Size: 54 583 889 1629 43 Fragments arranged by size: 1705 1629 1425 889 583 275 206 168 54 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3741 4546 1537 Size: 275 1929 805 4090 Fragments arranged by size: 4090 1929 805 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4109 4115 5029 1353 Size: 2756 6 914 3423 Fragments arranged by size: 3423 2756 914 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5686 6060 6206 387 Size: 1064 138 4097 374 146 1280 Fragments arranged by size: 4097 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3150 4325 5162 5759 6546 6814 1239 Size: 393 1518 1175 837 597 787 268 1524 Fragments arranged by size: 1524 1518 1175 837 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2155 3571 3615 4193 4233 2155 Size: 1416 44 578 40 5021 Fragments arranged by size: 5021 1416 578 44 40 EcoRI G'AATT_C Cuts at: 3069 4408 4786 3069 Size: 1339 378 5382 Fragments arranged by size: 5382 1339 378 FspI TGC'GCA Cuts at: 3878 5605 3878 Size: 1727 5372 FspAI rTGC'GCAy Cuts at: 3878 3878 Size: 7099 HaeII r_GCGC'y Cuts at: 1678 3429 3603 3641 6246 1678 Size: 1751 174 38 2605 2531 Fragments arranged by size: 2605 2531 1751 174 38 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2165 2197 2622 2642 2654 Size: 32 135 32 593 32 425 20 12 Cuts at: 2654 2674 3110 3142 3425 3457 3599 3631 3773 Size: 20 436 32 283 32 142 32 142 Cuts at: 3773 3805 3986 4018 5740 5772 5814 5846 1373 Size: 32 181 32 1722 32 42 32 2626 Fragments arranged by size: 2626 1722 593 436 425 283 181 142 142 135 42 32 32 32 32 32 32 32 32 32 32 20 20 12 HincII GTy'rAC Cuts at: 669 1657 1938 3463 4443 4689 669 Size: 988 281 1525 980 246 3079 Fragments arranged by size: 3079 1525 988 980 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7099 HpaI GTT'AAC Cuts at: 4689 4689 Size: 7099 KasI G'GCGC_C Cuts at: 3599 3599 Size: 7099 KpnI G_GTAC'C Cuts at: 2442 4424 2442 Size: 1982 5117 MfeI C'AATT_G Cuts at: 4698 4698 Size: 7099 MluI A'CGCG_T Cuts at: 6975 6975 Size: 7099 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2428 2470 2682 6213 6397 1305 Size: 1123 42 212 3531 184 2007 Fragments arranged by size: 3531 2007 1123 212 184 42 MscI TGG'CCA Cuts at: 10 64 1489 1832 3537 10 Size: 54 1425 343 1705 3572 Fragments arranged by size: 3572 1705 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2645 2696 3317 5057 5416 5575 518 Size: 648 1479 51 621 1740 359 159 2042 Fragments arranged by size: 2042 1740 1479 648 621 359 159 51 MspA1I CmG'CkG Cuts at: 1606 3612 3697 3820 4515 5192 6340 6585 1606 Size: 2006 85 123 695 677 1148 245 2120 Fragments arranged by size: 2120 2006 1148 695 677 245 123 85 NarI GG'CG_CC Cuts at: 3600 3600 Size: 7099 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5608 Fragments arranged by size: 5608 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7099 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7099 NotI GC'GGCC_GC Cuts at: 4546 4546 Size: 7099 NsiI A_TGCA'T Cuts at: 2857 2857 Size: 7099 NspI r_CATG'y Cuts at: 1132 1229 4457 6002 1132 Size: 97 3228 1545 2229 Fragments arranged by size: 3228 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7099 PciI A'CATG_T Cuts at: 5998 5998 Size: 7099 PflMI CCAn_nnn'nTGG Cuts at: 1108 2897 1108 Size: 1789 5310 PfoI T'CCnGG_A Cuts at: 2780 4280 2780 Size: 1500 5599 PmeI GTTT'AAAC Cuts at: 4403 4403 Size: 7099 PmlI CAC'GTG Cuts at: 2189 3982 2189 Size: 1793 5306 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5168 5200 6681 6713 5168 Size: 32 1481 32 5554 Fragments arranged by size: 5554 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2155 3615 4193 4233 2155 Size: 1460 578 40 5021 Fragments arranged by size: 5021 1460 578 40 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7099 PsiI TTA'TAA Cuts at: 4669 4669 Size: 7099 PstI C_TGCA'G Cuts at: 830 3702 4451 830 Size: 2872 749 3478 Fragments arranged by size: 3478 2872 749 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7099 PvuII CAG'CTG Cuts at: 3820 3820 Size: 7099 SacII CC_GC'GG Cuts at: 3613 3613 Size: 7099 SalI G'TCGA_C Cuts at: 1936 4441 1936 Size: 2505 4594 SanDI GG'GwC_CC Cuts at: 3615 4193 4233 3615 Size: 578 40 6481 Fragments arranged by size: 6481 578 40 SapI GCTCTTCn'nnn_ Cuts at: 4109 4109 Size: 7099 SbfI CC_TGCA'GG Cuts at: 4451 4451 Size: 7099 ScaI AGT'ACT Cuts at: 5347 7059 5347 Size: 1712 5387 SexAI A'CCwGG_T Cuts at: 3756 4071 3756 Size: 315 6784 SfcI C'TryA_G Cuts at: 826 1045 3575 3698 4447 5582 6263 6454 826 Size: 219 2530 123 749 1135 681 191 1471 Fragments arranged by size: 2530 1471 1135 749 681 219 191 123 SfoI GGC'GCC Cuts at: 3601 3601 Size: 7099 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7099 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7099 SmaI CCC'GGG Cuts at: 1872 2198 3737 3841 4426 1872 Size: 326 1539 104 585 4545 Fragments arranged by size: 4545 1539 585 326 104 SmlI C'TyrA_G Cuts at: 820 1017 1943 2475 3824 5203 6104 6366 820 Size: 197 926 532 1349 1379 901 262 1553 Fragments arranged by size: 1553 1379 1349 926 901 532 262 197 SnaBI TAC'GTA Cuts at: 493 4890 493 Size: 4397 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7099 SphI G_CATG'C Cuts at: 1132 4457 1132 Size: 3325 3774 SrfI GCCC'GGGC Cuts at: 3841 3841 Size: 7099 SspI AAT'ATT Cuts at: 5 52 2052 2959 5023 5 Size: 47 2000 907 2064 2081 Fragments arranged by size: 2081 2064 2000 907 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7099 StyI C'CwwG_G Cuts at: 513 1065 2004 2158 2773 3510 3765 4538 513 Size: 552 939 154 615 737 255 773 3074 Fragments arranged by size: 3074 939 773 737 615 552 255 154 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2292 3304 3374 5126 5143 5296 5481 Size: 1255 712 1012 70 1752 17 153 185 Cuts at: 5481 5926 325 Size: 445 1498 Fragments arranged by size: 1752 1498 1255 1012 712 445 185 153 70 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3252 3306 5345 Size: 274 80 33 51 1443 1275 54 2039 Cuts at: 5345 7057 96 Size: 1712 138 Fragments arranged by size: 2039 1712 1443 1275 274 138 80 54 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7059 7091 7059 Size: 32 7067 Tth111I GACn'n_nGTC Cuts at: 1316 4237 1316 Size: 2921 4178 XbaI T'CTAG_A Cuts at: 2404 4435 2404 Size: 2031 5068 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3919 3919 Size: 7099 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7099 XmaI C'CCGG_G Cuts at: 1870 2196 3735 3839 4424 1870 Size: 326 1539 104 585 4545 Fragments arranged by size: 4545 1539 585 326 104 XmnI GAAnn'nnTTC Cuts at: 2865 3203 5228 2865 Size: 338 2025 4736 Fragments arranged by size: 4736 2025 338 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3334 4907 276 Size: 53 83 186 936 1800 1573 2468 Fragments arranged by size: 2468 1800 1573 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BbvCI BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI NaeI NgoMIV NruI PacI PspOMI PspXI PsrI RsrII SacI SfiI SwaI