(Circular) (Six-base) MAPSORT of: pFN21AB4188.seq Check: 7181 from: 1 to: 5776 pFN21AB4188 5776 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3586 278 Size: 53 83 186 936 2050 2468 Fragments arranged by size: 2468 2050 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3119 1937 Size: 1182 4594 Acc65I G'GTAC_C Cuts at: 3097 3097 Size: 5776 AclI AA'CG_TT Cuts at: 1563 3903 4276 1563 Size: 2340 373 3063 Fragments arranged by size: 3063 2340 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2124 2348 3081 3839 5223 5491 1632 Size: 492 224 733 758 1384 268 1917 Fragments arranged by size: 1917 1384 758 733 492 268 224 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5579 AflIII A'CryG_T Cuts at: 1203 4675 5652 1203 Size: 3472 977 1327 Fragments arranged by size: 3472 1327 977 AgeI A'CCGG_T Cuts at: 4607 4607 Size: 5776 AhdI GACnn_n'nnGTC Cuts at: 4505 4505 Size: 5776 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5776 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3009 1184 Size: 1825 3951 AlwNI CAG_nnn'CTG Cuts at: 3519 5091 3519 Size: 1572 4204 ApaI G_GGCC'C Cuts at: 3050 3050 Size: 5776 ApaLI G'TGCA_C Cuts at: 3833 4989 3833 Size: 1156 4620 ApoI r'AATT_y Cuts at: 1493 2336 3027 3085 3311 3463 5572 1493 Size: 843 691 58 226 152 2109 1697 Fragments arranged by size: 2109 1697 843 691 226 152 58 AseI AT'TA_AT Cuts at: 160 1032 4330 160 Size: 872 3298 1606 Fragments arranged by size: 3298 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5776 AvaI C'yCGr_G Cuts at: 1870 1943 2574 3101 1870 Size: 73 631 527 4545 Fragments arranged by size: 4545 631 527 73 BamHI G'GATC_C Cuts at: 3106 3473 3106 Size: 367 5409 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3097 4552 618 Size: 325 217 627 1310 1455 1842 Fragments arranged by size: 1842 1455 1310 627 325 217 BanII G_rGCy'C Cuts at: 1391 2492 3050 3095 1391 Size: 1101 558 45 4072 Fragments arranged by size: 4072 1101 558 45 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3040 928 Size: 971 1141 3664 Fragments arranged by size: 3664 1141 971 BbvCI CC'TCA_GC Cuts at: 2454 2454 Size: 5776 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3967 4001 1903 Size: 34 2030 34 3678 Fragments arranged by size: 3678 2030 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2801 3668 4878 2801 Size: 867 1210 3699 Fragments arranged by size: 3699 1210 867 BclI T'GATC_A Cuts at: 1548 1548 Size: 5776 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2588 4387 136 Size: 107 122 71 631 605 916 1799 1525 Fragments arranged by size: 1799 1525 916 631 605 122 107 71 BglII A'GATC_T Cuts at: 2049 5771 2049 Size: 3722 2054 BlpI GC'TnA_GC Cuts at: 3193 3193 Size: 5776 Bme1580I G_kGCm'C Cuts at: 1790 3050 3837 4993 1790 Size: 1260 787 1156 2573 Fragments arranged by size: 2573 1260 1156 787 BmgBI CAC'GTC Cuts at: 1318 2521 1318 Size: 1203 4573 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3507 4465 442 Size: 416 53 83 399 24 2090 958 1753 Fragments arranged by size: 2090 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5776 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2256 4436 1239 Size: 1017 2180 2579 Fragments arranged by size: 2579 2180 1017 Bpu10I CC'TnA_GC Cuts at: 2454 2454 Size: 5776 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3901 4766 5064 3901 Size: 865 298 4613 Fragments arranged by size: 4613 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2274 4439 882 Size: 617 775 2165 2219 Fragments arranged by size: 2219 2165 775 617 BsaAI yAC'GTr Cuts at: 493 1204 3567 493 Size: 711 2363 2702 Fragments arranged by size: 2702 2363 711 BsaBI GATnn'nnATC Cuts at: 2477 5770 2477 Size: 3293 2483 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3583 3965 4600 275 Size: 53 83 186 936 2050 382 635 1451 Fragments arranged by size: 2050 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4209 4607 4881 5028 5439 4209 Size: 398 274 147 411 4546 Fragments arranged by size: 4546 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5551 Fragments arranged by size: 5551 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5776 BseYI C'CCAG_C Cuts at: 3049 4979 5475 3049 Size: 1930 496 3350 Fragments arranged by size: 3350 1930 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2648 1163 Size: 573 912 4291 Fragments arranged by size: 4291 912 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3226 3987 5015 1540 Size: 275 186 1225 761 1028 2301 Fragments arranged by size: 2301 1225 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3837 3922 4993 3837 Size: 85 1071 4620 Fragments arranged by size: 4620 1071 85 BsmI GAATG_Cn' Cuts at: 2239 3287 3380 2239 Size: 1048 93 4635 Fragments arranged by size: 4635 1048 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2492 3050 3095 3837 3922 4993 1391 Size: 399 702 558 45 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 702 558 399 85 45 BspEI T'CCGG_A Cuts at: 5439 5439 Size: 5776 BspHI T'CATG_A Cuts at: 3665 5395 3665 Size: 1730 4046 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3131 844 Size: 2287 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2830 3663 1394 Size: 429 1007 833 3507 Fragments arranged by size: 3507 1007 833 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2259 4271 4445 65 Size: 1183 1011 2012 174 1396 Fragments arranged by size: 2012 1396 1183 1011 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2018 4420 4607 1700 Size: 110 208 2402 187 2869 Fragments arranged by size: 2869 2402 208 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5776 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5776 BssSI C'ACGA_G Cuts at: 3836 4848 3836 Size: 1012 4764 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5776 BstBI TT'CG_AA Cuts at: 3031 4580 3031 Size: 1549 4227 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5776 BstYI r'GATC_y Cuts at: 1972 2049 3106 3473 3858 3875 5327 5413 5425 Size: 77 1057 367 385 17 1452 86 12 Cuts at: 5425 5771 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1057 385 367 346 86 77 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5224 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5037 Fragments arranged by size: 5037 683 56 BtsI GCAGTG_nn' Cuts at: 3292 4086 4106 3292 Size: 794 20 4962 Fragments arranged by size: 4962 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5776 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5741 DraI TTT'AAA Cuts at: 3080 3080 Size: 5776 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4783 809 Size: 507 3467 1802 Fragments arranged by size: 3467 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2347 2667 3223 Size: 54 1425 50 275 18 517 320 556 Cuts at: 3223 4112 5741 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 556 517 320 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3223 1537 Size: 275 1411 4090 Fragments arranged by size: 4090 1411 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3706 1353 Size: 2353 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4363 4737 4883 387 Size: 1064 138 2774 374 146 1280 Fragments arranged by size: 2774 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2124 2256 2348 3081 3839 4436 5223 Size: 393 492 132 92 733 758 597 787 Cuts at: 5223 5491 1239 Size: 268 1524 Fragments arranged by size: 1524 787 758 733 597 492 393 268 132 92 EcoNI CCTnn'n_nnAGG Cuts at: 2186 2186 Size: 5776 EcoO109I rG'GnC_Cy Cuts at: 3008 3046 3008 Size: 38 5738 EcoRI G'AATT_C Cuts at: 3027 3085 3463 3027 Size: 58 378 5340 Fragments arranged by size: 5340 378 58 FspI TGC'GCA Cuts at: 4282 4282 Size: 5776 HaeII r_GCGC'y Cuts at: 1678 4923 1678 Size: 3245 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4417 4449 4491 4523 1373 Size: 32 135 32 2845 32 42 32 2626 Fragments arranged by size: 2845 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2934 3120 3366 669 Size: 988 281 996 186 246 3079 Fragments arranged by size: 3079 996 988 281 246 186 HindIII A'AGCT_T Cuts at: 748 748 Size: 5776 HpaI GTT'AAC Cuts at: 3366 3366 Size: 5776 KpnI G_GTAC'C Cuts at: 3101 3101 Size: 5776 MfeI C'AATT_G Cuts at: 3375 3375 Size: 5776 MluI A'CGCG_T Cuts at: 5652 5652 Size: 5776 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2166 2613 4890 5074 1305 Size: 861 447 2277 184 2007 Fragments arranged by size: 2277 2007 861 447 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2349 2669 10 Size: 54 1425 343 517 320 3117 Fragments arranged by size: 3117 1425 517 343 320 54 MslI CAynn'nnrTG Cuts at: 518 1166 2283 2757 3734 4093 4252 518 Size: 648 1117 474 977 359 159 2042 Fragments arranged by size: 2042 1117 977 648 474 359 159 MspA1I CmG'CkG Cuts at: 1606 2453 3053 3192 3869 5017 5262 1606 Size: 847 600 139 677 1148 245 2120 Fragments arranged by size: 2120 1148 847 677 600 245 139 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5224 NdeI CA'TA_TG Cuts at: 387 387 Size: 5776 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5776 NotI GC'GGCC_GC Cuts at: 3223 3223 Size: 5776 NspI r_CATG'y Cuts at: 1132 1229 2817 2978 3134 4679 1132 Size: 97 1588 161 156 1545 2229 Fragments arranged by size: 2229 1588 1545 161 156 97 PasI CC'CwG_GG Cuts at: 1348 2239 1348 Size: 891 4885 PciI A'CATG_T Cuts at: 4675 4675 Size: 5776 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5776 PmeI GTTT'AAAC Cuts at: 3080 3080 Size: 5776 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3845 3877 5358 5390 3845 Size: 32 1481 32 4231 Fragments arranged by size: 4231 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3008 3008 Size: 5776 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5776 PsiI TTA'TAA Cuts at: 2660 3346 2660 Size: 686 5090 PspOMI G'GGCC_C Cuts at: 3046 3046 Size: 5776 PstI C_TGCA'G Cuts at: 830 2452 2941 3016 3128 830 Size: 1622 489 75 112 3478 Fragments arranged by size: 3478 1622 489 112 75 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5776 PvuII CAG'CTG Cuts at: 2453 2453 Size: 5776 SalI G'TCGA_C Cuts at: 1936 3118 1936 Size: 1182 4594 SbfI CC_TGCA'GG Cuts at: 3016 3128 3016 Size: 112 5664 ScaI AGT'ACT Cuts at: 4024 5736 4024 Size: 1712 4064 SfcI C'TryA_G Cuts at: 826 1045 2448 2937 3012 3124 4259 4940 5131 Size: 219 1403 489 75 112 1135 681 191 Cuts at: 5131 826 Size: 1471 Fragments arranged by size: 1471 1403 1135 681 489 219 191 112 75 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5776 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5776 SmaI CCC'GGG Cuts at: 1872 3103 1872 Size: 1231 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2574 3880 4781 5043 820 Size: 197 926 631 1306 901 262 1553 Fragments arranged by size: 1553 1306 926 901 631 262 197 SnaBI TAC'GTA Cuts at: 493 3567 493 Size: 3074 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5776 SphI G_CATG'C Cuts at: 1132 2978 3134 1132 Size: 1846 156 3774 Fragments arranged by size: 3774 1846 156 SspI AAT'ATT Cuts at: 5 52 3700 5 Size: 47 3648 2081 Fragments arranged by size: 3648 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5776 StyI C'CwwG_G Cuts at: 513 1065 2715 3215 513 Size: 552 1650 500 3074 Fragments arranged by size: 3074 1650 552 500 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2513 3803 3820 3973 4158 4603 325 Size: 1255 933 1290 17 153 185 445 1498 Fragments arranged by size: 1498 1290 1255 933 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2076 2959 4022 Size: 274 80 33 51 1443 99 883 1063 Cuts at: 4022 5734 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1063 883 274 138 99 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5736 5768 5736 Size: 32 5744 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5776 XbaI T'CTAG_A Cuts at: 3112 3112 Size: 5776 XhoI C'TCGA_G Cuts at: 1943 2574 1943 Size: 631 5145 XmaI C'CCGG_G Cuts at: 1870 3101 1870 Size: 1231 4545 XmnI GAAnn'nnTTC Cuts at: 2583 3905 2583 Size: 1322 4454 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3584 276 Size: 53 83 186 936 2050 2468 Fragments arranged by size: 2468 2050 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI