(Circular) (Six-base) MAPSORT of: pFN21AB4176.seq Check: 3724 from: 1 to: 6058 pFN21AB4176 6058 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3868 278 Size: 53 83 186 936 2332 2468 Fragments arranged by size: 2468 2332 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2471 3401 1937 Size: 534 930 4594 Fragments arranged by size: 4594 930 534 Acc65I G'GTAC_C Cuts at: 3293 3379 3293 Size: 86 5972 AclI AA'CG_TT Cuts at: 1563 2305 4185 4558 1563 Size: 742 1880 373 3063 Fragments arranged by size: 3063 1880 742 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2223 2562 3240 4121 5505 5773 1632 Size: 591 339 678 881 1384 268 1917 Fragments arranged by size: 1917 1384 881 678 591 339 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5861 AflIII A'CryG_T Cuts at: 1203 4957 5934 1203 Size: 3754 977 1327 Fragments arranged by size: 3754 1327 977 AgeI A'CCGG_T Cuts at: 4889 4889 Size: 6058 AhdI GACnn_n'nnGTC Cuts at: 3262 4787 3262 Size: 1525 4533 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6058 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6058 AlwNI CAG_nnn'CTG Cuts at: 2041 3113 3801 5373 2041 Size: 1072 688 1572 2726 Fragments arranged by size: 2726 1572 1072 688 ApaLI G'TGCA_C Cuts at: 4115 5271 4115 Size: 1156 4902 ApoI r'AATT_y Cuts at: 1493 2136 2312 2445 2552 2908 2993 3367 3593 Size: 643 176 133 107 356 85 374 226 Cuts at: 3593 3745 5854 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 643 374 356 226 176 152 133 107 85 AseI AT'TA_AT Cuts at: 160 1032 4612 160 Size: 872 3580 1606 Fragments arranged by size: 3580 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6058 AvaI C'yCGr_G Cuts at: 1870 1943 3383 1870 Size: 73 1440 4545 Fragments arranged by size: 4545 1440 73 BamHI G'GATC_C Cuts at: 3388 3755 3388 Size: 367 5691 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3293 3379 4834 618 Size: 325 217 627 1506 86 1455 1842 Fragments arranged by size: 1842 1506 1455 627 325 217 86 BanII G_rGCy'C Cuts at: 1391 3377 1391 Size: 1986 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2015 2200 928 Size: 971 116 185 4786 Fragments arranged by size: 4786 971 185 116 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4249 4283 1903 Size: 34 2312 34 3678 Fragments arranged by size: 3678 2312 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3950 5160 3950 Size: 1210 4848 BclI T'GATC_A Cuts at: 1548 1548 Size: 6058 BfrBI ATG'CAT Cuts at: 2567 2609 2969 2567 Size: 42 360 5656 Fragments arranged by size: 5656 360 42 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4669 136 Size: 107 122 71 631 605 2997 1525 Fragments arranged by size: 2997 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6053 6053 Size: 6058 BlpI GC'TnA_GC Cuts at: 3475 3475 Size: 6058 Bme1580I G_kGCm'C Cuts at: 1790 4119 5275 1790 Size: 2329 1156 2573 Fragments arranged by size: 2573 2329 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6058 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2195 3322 3789 Size: 416 53 83 399 24 778 1127 467 Cuts at: 3789 4747 442 Size: 958 1753 Fragments arranged by size: 1753 1127 958 778 467 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2361 1056 Size: 1305 4753 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2358 4718 1239 Size: 1119 2360 2579 Fragments arranged by size: 2579 2360 1119 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4183 5048 5346 4183 Size: 865 298 4895 Fragments arranged by size: 4895 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4721 882 Size: 617 3222 2219 Fragments arranged by size: 3222 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2224 3849 493 Size: 711 1020 1625 2702 Fragments arranged by size: 2702 1625 1020 711 BsaBI GATnn'nnATC Cuts at: 6052 6052 Size: 6058 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3865 4247 4882 275 Size: 53 83 186 936 2332 382 635 1451 Fragments arranged by size: 2332 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4491 4889 5163 5310 5721 4491 Size: 398 274 147 411 4828 Fragments arranged by size: 4828 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5833 Fragments arranged by size: 5833 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6058 BseYI C'CCAG_C Cuts at: 2665 5261 5757 2665 Size: 2596 496 2966 Fragments arranged by size: 2966 2596 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5485 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3508 4269 5297 1540 Size: 275 186 1507 761 1028 2301 Fragments arranged by size: 2301 1507 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2905 4119 4204 5275 2905 Size: 1214 85 1071 3688 Fragments arranged by size: 3688 1214 1071 85 BsmI GAATG_Cn' Cuts at: 2504 3569 3662 2504 Size: 1065 93 4900 Fragments arranged by size: 4900 1065 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2905 3377 4119 4204 5275 1391 Size: 399 1115 472 742 85 1071 2174 Fragments arranged by size: 2174 1115 1071 742 472 399 85 BspEI T'CCGG_A Cuts at: 5721 5721 Size: 6058 BspHI T'CATG_A Cuts at: 2790 3947 5677 2790 Size: 1157 1730 3171 Fragments arranged by size: 3171 1730 1157 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2670 3413 844 Size: 1826 743 3489 Fragments arranged by size: 3489 1826 743 BsrBI CCG'CTC Cuts at: 1394 1823 3945 1394 Size: 429 2122 3507 Fragments arranged by size: 3507 2122 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4553 4727 65 Size: 1183 3305 174 1396 Fragments arranged by size: 3305 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4702 4889 1700 Size: 110 2892 187 2869 Fragments arranged by size: 2892 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6058 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6058 BssSI C'ACGA_G Cuts at: 4118 5130 4118 Size: 1012 5046 BstAPI GCAn_nnn'nTGC Cuts at: 794 2041 794 Size: 1247 4811 BstBI TT'CG_AA Cuts at: 2443 4862 2443 Size: 2419 3639 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6058 BstYI r'GATC_y Cuts at: 1972 3145 3388 3755 4140 4157 5609 5695 5707 Size: 1173 243 367 385 17 1452 86 12 Cuts at: 5707 6053 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1173 385 367 346 243 86 17 12 BstZ17I GTA'TAC Cuts at: 2472 2472 Size: 6058 BtgI C'CryG_G Cuts at: 513 1065 2940 513 Size: 552 1875 3631 Fragments arranged by size: 3631 1875 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5319 Fragments arranged by size: 5319 683 56 BtsI GCAGTG_nn' Cuts at: 3114 3574 4368 4388 3114 Size: 460 794 20 4784 Fragments arranged by size: 4784 794 460 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6058 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6023 DraI TTT'AAA Cuts at: 2310 2317 3327 3362 2310 Size: 7 1010 35 5006 Fragments arranged by size: 5006 1010 35 7 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5065 809 Size: 507 3749 1802 Fragments arranged by size: 3749 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3505 4394 6023 Size: 54 1425 50 275 18 1675 889 1629 Cuts at: 6023 8 Size: 43 Fragments arranged by size: 1675 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3505 1537 Size: 275 1693 4090 Fragments arranged by size: 4090 1693 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3038 3988 1353 Size: 1685 950 3423 Fragments arranged by size: 3423 1685 950 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4645 5019 5165 387 Size: 1064 138 3056 374 146 1280 Fragments arranged by size: 3056 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2223 2358 2562 3240 4121 4718 5505 Size: 393 591 135 204 678 881 597 787 Cuts at: 5505 5773 1239 Size: 268 1524 Fragments arranged by size: 1524 881 787 678 597 591 393 268 204 135 EcoNI CCTnn'n_nnAGG Cuts at: 3342 3342 Size: 6058 EcoO109I rG'GnC_Cy Cuts at: 2065 2321 2065 Size: 256 5802 EcoRI G'AATT_C Cuts at: 2136 2445 2908 3367 3745 2136 Size: 309 463 459 378 4449 Fragments arranged by size: 4449 463 459 378 309 FspI TGC'GCA Cuts at: 4564 4564 Size: 6058 HaeII r_GCGC'y Cuts at: 1678 5205 1678 Size: 3527 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3248 3280 4699 4731 4773 Size: 32 135 32 1676 32 1419 32 42 Cuts at: 4773 4805 1373 Size: 32 2626 Fragments arranged by size: 2626 1676 1419 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3402 3648 669 Size: 988 281 1464 246 3079 Fragments arranged by size: 3079 1464 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6058 HpaI GTT'AAC Cuts at: 3648 3648 Size: 6058 KpnI G_GTAC'C Cuts at: 3297 3383 3297 Size: 86 5972 MfeI C'AATT_G Cuts at: 2268 3657 2268 Size: 1389 4669 MluI A'CGCG_T Cuts at: 5934 5934 Size: 6058 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5172 5356 1305 Size: 3867 184 2007 Fragments arranged by size: 3867 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4236 Fragments arranged by size: 4236 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2222 4016 4375 4534 518 Size: 648 1056 1794 359 159 2042 Fragments arranged by size: 2042 1794 1056 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3218 3474 4151 5299 5544 1606 Size: 1612 256 677 1148 245 2120 Fragments arranged by size: 2120 1612 1148 677 256 245 NcoI C'CATG_G Cuts at: 513 1065 2940 513 Size: 552 1875 3631 Fragments arranged by size: 3631 1875 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6058 NheI G'CTAG_C Cuts at: 1052 2357 1052 Size: 1305 4753 NotI GC'GGCC_GC Cuts at: 3505 3505 Size: 6058 NsiI A_TGCA'T Cuts at: 2569 2611 2971 2569 Size: 42 360 5656 Fragments arranged by size: 5656 360 42 NspI r_CATG'y Cuts at: 1132 1229 2035 3416 4961 1132 Size: 97 806 1381 1545 2229 Fragments arranged by size: 2229 1545 1381 806 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6058 PciI A'CATG_T Cuts at: 4957 4957 Size: 6058 PflMI CCAn_nnn'nTGG Cuts at: 1108 2946 1108 Size: 1838 4220 PmeI GTTT'AAAC Cuts at: 3362 3362 Size: 6058 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4127 4159 5640 5672 4127 Size: 32 1481 32 4513 Fragments arranged by size: 4513 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2065 2321 2065 Size: 256 5802 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6058 PsiI TTA'TAA Cuts at: 3628 3628 Size: 6058 PstI C_TGCA'G Cuts at: 830 3410 830 Size: 2580 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6058 PvuII CAG'CTG Cuts at: 3218 3218 Size: 6058 SalI G'TCGA_C Cuts at: 1936 3400 1936 Size: 1464 4594 SanDI GG'GwC_CC Cuts at: 2065 2065 Size: 6058 SbfI CC_TGCA'GG Cuts at: 3410 3410 Size: 6058 ScaI AGT'ACT Cuts at: 4306 6018 4306 Size: 1712 4346 SfcI C'TryA_G Cuts at: 826 1045 3406 4541 5222 5413 826 Size: 219 2361 1135 681 191 1471 Fragments arranged by size: 2361 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6058 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6058 SmaI CCC'GGG Cuts at: 1872 3385 1872 Size: 1513 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4162 5063 5325 820 Size: 197 926 2219 901 262 1553 Fragments arranged by size: 2219 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3849 493 Size: 3356 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6058 SphI G_CATG'C Cuts at: 1132 3416 1132 Size: 2284 3774 SspI AAT'ATT Cuts at: 5 52 3982 5 Size: 47 3930 2081 Fragments arranged by size: 3930 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6058 StyI C'CwwG_G Cuts at: 513 1065 2940 3497 513 Size: 552 1875 557 3074 Fragments arranged by size: 3074 1875 557 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4085 4102 4255 4440 4885 325 Size: 1255 2505 17 153 185 445 1498 Fragments arranged by size: 2505 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2620 2702 4304 Size: 274 80 33 51 1443 643 82 1602 Cuts at: 4304 6016 96 Size: 1712 138 Fragments arranged by size: 1712 1602 1443 643 274 138 82 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6018 6050 6018 Size: 32 6026 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6058 XbaI T'CTAG_A Cuts at: 3394 3394 Size: 6058 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2080 2080 Size: 6058 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6058 XmaI C'CCGG_G Cuts at: 1870 3383 1870 Size: 1513 4545 XmnI GAAnn'nnTTC Cuts at: 3233 4187 3233 Size: 954 5104 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3866 276 Size: 53 83 186 936 2332 2468 Fragments arranged by size: 2468 2332 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI