(Circular) (Six-base) MAPSORT of: pFN21AB4151.seq Check: 4724 from: 1 to: 7879 pFN21AB4151 7879 bp With 182 enzymes: SgfI * October 20, 2009 10:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3817 3817 Size: 7879 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5689 278 Size: 53 83 186 936 4153 2468 Fragments arranged by size: 4153 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3771 5222 1937 Size: 1834 1451 4594 Fragments arranged by size: 4594 1834 1451 Acc65I G'GTAC_C Cuts at: 5200 5200 Size: 7879 AclI AA'CG_TT Cuts at: 1563 6006 6379 1563 Size: 4443 373 3063 Fragments arranged by size: 4443 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3926 5942 7326 7594 1632 Size: 2294 2016 1384 268 1917 Fragments arranged by size: 2294 2016 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7682 AflIII A'CryG_T Cuts at: 1203 6778 7755 1203 Size: 5575 977 1327 Fragments arranged by size: 5575 1327 977 AgeI A'CCGG_T Cuts at: 6710 6710 Size: 7879 AhdI GACnn_n'nnGTC Cuts at: 6608 6608 Size: 7879 AleI CACnn'nnGTG Cuts at: 1166 2547 1166 Size: 1381 6498 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4106 1184 Size: 2922 4957 AlwNI CAG_nnn'CTG Cuts at: 2942 3010 4180 4444 5622 7194 2942 Size: 68 1170 264 1178 1572 3627 Fragments arranged by size: 3627 1572 1178 1170 264 68 ApaI G_GGCC'C Cuts at: 4239 4239 Size: 7879 ApaLI G'TGCA_C Cuts at: 5936 7092 5936 Size: 1156 6723 ApoI r'AATT_y Cuts at: 1493 4397 5188 5414 5566 7675 1493 Size: 2904 791 226 152 2109 1697 Fragments arranged by size: 2904 2109 1697 791 226 152 AseI AT'TA_AT Cuts at: 160 1032 6433 160 Size: 872 5401 1606 Fragments arranged by size: 5401 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7879 AvaI C'yCGr_G Cuts at: 1870 1943 3105 3948 5204 1870 Size: 73 1162 843 1256 4545 Fragments arranged by size: 4545 1256 1162 843 73 BamHI G'GATC_C Cuts at: 5209 5576 5209 Size: 367 7512 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2110 3187 3809 4749 5200 Size: 325 217 627 323 1077 622 940 451 Cuts at: 5200 6655 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1077 940 627 622 451 325 323 217 BanII G_rGCy'C Cuts at: 1391 2418 3112 3949 4239 4491 5198 1391 Size: 1027 694 837 290 252 707 4072 Fragments arranged by size: 4072 1027 837 707 694 290 252 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6908 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3929 3963 6070 6104 1903 Size: 34 1992 34 2107 34 3678 Fragments arranged by size: 3678 2107 1992 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5771 6981 5771 Size: 1210 6669 BclI T'GATC_A Cuts at: 1548 1548 Size: 7879 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2247 2330 2933 Size: 107 122 71 631 605 575 83 603 Cuts at: 2933 3046 6490 136 Size: 113 3444 1525 Fragments arranged by size: 3444 1525 631 605 603 575 122 113 107 83 71 BglII A'GATC_T Cuts at: 7874 7874 Size: 7879 BlpI GC'TnA_GC Cuts at: 2412 3027 5296 2412 Size: 615 2269 4995 Fragments arranged by size: 4995 2269 615 Bme1580I G_kGCm'C Cuts at: 1790 2106 2321 3812 4239 4752 5940 7096 1790 Size: 316 215 1491 427 513 1188 1156 2573 Fragments arranged by size: 2573 1491 1188 1156 513 427 316 215 BmgBI CAC'GTC Cuts at: 1318 4835 1318 Size: 3517 4362 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2077 2320 2341 Size: 416 53 83 399 24 660 243 21 Cuts at: 2341 3815 4147 5610 6568 442 Size: 1474 332 1463 958 1753 Fragments arranged by size: 1753 1474 1463 958 660 416 399 332 243 83 53 24 21 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7879 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2198 2531 3323 3812 4088 4745 6539 1239 Size: 959 333 792 489 276 657 1794 2579 Fragments arranged by size: 2579 1794 959 792 657 489 333 276 Bpu10I CC'TnA_GC Cuts at: 3085 4213 3085 Size: 1128 6751 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3311 3440 5009 5132 6004 6869 7167 3311 Size: 129 1569 123 872 865 298 4023 Fragments arranged by size: 4023 1569 872 865 298 129 123 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6542 882 Size: 617 5043 2219 Fragments arranged by size: 5043 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5095 5670 493 Size: 711 3891 575 2702 Fragments arranged by size: 3891 2702 711 575 BsaBI GATnn'nnATC Cuts at: 7873 7873 Size: 7879 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4416 5686 6068 6703 Size: 53 83 186 936 2883 1270 382 635 Cuts at: 6703 275 Size: 1451 Fragments arranged by size: 2883 1451 1270 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3153 3777 6312 6710 6984 7131 7542 3153 Size: 624 2535 398 274 147 411 3490 Fragments arranged by size: 3490 2535 624 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2732 2762 2862 2892 1178 Size: 30 165 30 1329 30 100 30 6165 Fragments arranged by size: 6165 1329 165 100 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2378 3074 4499 4922 1186 Size: 1192 696 1425 423 4143 Fragments arranged by size: 4143 1425 1192 696 423 BseYI C'CCAG_C Cuts at: 2985 3176 3400 3478 4505 4678 5083 7082 7578 Size: 191 224 78 1027 173 405 1999 496 Cuts at: 7578 2985 Size: 3286 Fragments arranged by size: 3286 1999 1027 496 405 224 191 173 78 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2975 2996 3143 3845 4097 4166 4406 Size: 573 1239 21 147 702 252 69 240 Cuts at: 4406 4763 1163 Size: 357 4279 Fragments arranged by size: 4279 1239 702 573 357 252 240 147 69 21 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5329 6090 7118 1540 Size: 275 186 3328 761 1028 2301 Fragments arranged by size: 3328 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2418 2496 3028 3933 4491 4953 5940 6025 7096 Size: 78 532 905 558 462 987 85 1071 Cuts at: 7096 2418 Size: 3201 Fragments arranged by size: 3201 1071 987 905 558 532 462 85 78 BsmI GAATG_Cn' Cuts at: 3106 5390 5483 3106 Size: 2284 93 5502 Fragments arranged by size: 5502 2284 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2106 2321 2418 2496 3028 3112 3812 Size: 399 316 215 97 78 532 84 700 Cuts at: 3812 3933 3949 4239 4491 4752 4953 5198 5940 Size: 121 16 290 252 261 201 245 742 Cuts at: 5940 6025 7096 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 700 532 399 316 290 261 252 245 215 201 121 97 85 84 78 16 BspEI T'CCGG_A Cuts at: 3153 7542 3153 Size: 4389 3490 BspHI T'CATG_A Cuts at: 3231 5768 7498 3231 Size: 2537 1730 3612 Fragments arranged by size: 3612 2537 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2364 2711 3396 3817 4378 5234 844 Size: 1520 347 685 421 561 856 3489 Fragments arranged by size: 3489 1520 856 685 561 421 347 BsrBI CCG'CTC Cuts at: 1394 1823 2736 4258 5136 5766 1394 Size: 429 913 1522 878 630 3507 Fragments arranged by size: 3507 1522 913 878 630 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2208 3061 3826 4683 6374 6548 65 Size: 1183 960 853 765 857 1691 174 1396 Fragments arranged by size: 1691 1396 1183 960 857 853 765 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3036 4641 6523 6710 1700 Size: 110 1226 1605 1882 187 2869 Fragments arranged by size: 2869 1882 1605 1226 187 110 BsrGI T'GTAC_A Cuts at: 96 3750 4731 96 Size: 3654 981 3244 Fragments arranged by size: 3654 3244 981 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7879 BssSI C'ACGA_G Cuts at: 5939 6951 5939 Size: 1012 6867 BstAPI GCAn_nnn'nTGC Cuts at: 794 3937 4180 4384 794 Size: 3143 243 204 4289 Fragments arranged by size: 4289 3143 243 204 BstBI TT'CG_AA Cuts at: 6683 6683 Size: 7879 BstEII G'GTnAC_C Cuts at: 1184 2674 2698 1184 Size: 1490 24 6365 Fragments arranged by size: 6365 1490 24 BstXI CCAn_nnnn'nTGG Cuts at: 2125 2230 3727 4021 4110 2125 Size: 105 1497 294 89 5894 Fragments arranged by size: 5894 1497 294 105 89 BstYI r'GATC_y Cuts at: 1972 3181 4422 5209 5576 5961 5978 7430 7516 Size: 1209 1241 787 367 385 17 1452 86 Cuts at: 7516 7528 7874 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1241 1209 787 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3303 3303 Size: 7879 BtgI C'CryG_G Cuts at: 513 1065 2548 2796 2866 4704 513 Size: 552 1483 248 70 1838 3688 Fragments arranged by size: 3688 1838 1483 552 248 70 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7140 Fragments arranged by size: 7140 683 56 BtsI GCAGTG_nn' Cuts at: 2146 2319 2436 3126 3275 3785 3947 4031 5395 Size: 173 117 690 149 510 162 84 1364 Cuts at: 5395 6189 6209 2146 Size: 794 20 3816 Fragments arranged by size: 3816 1364 794 690 510 173 162 149 117 84 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7879 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7844 DraI TTT'AAA Cuts at: 2914 5183 2914 Size: 2269 5610 DraIII CAC_nnn'GTG Cuts at: 2657 2657 Size: 7879 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3314 6886 809 Size: 507 1998 3572 1802 Fragments arranged by size: 3572 1998 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2127 3034 3038 Size: 54 1425 50 275 18 297 907 4 Cuts at: 3038 3717 4365 5326 6215 7844 8 Size: 679 648 961 889 1629 43 Fragments arranged by size: 1629 1425 961 907 889 679 648 297 275 54 50 43 18 4 EagI C'GGCC_G Cuts at: 1537 1812 5326 1537 Size: 275 3514 4090 Fragments arranged by size: 4090 3514 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2603 3974 5809 1353 Size: 1250 1371 1835 3423 Fragments arranged by size: 3423 1835 1371 1250 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6466 6840 6986 387 Size: 1064 138 4877 374 146 1280 Fragments arranged by size: 4877 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2416 4489 2416 Size: 2073 5806 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2198 2531 3323 3812 3926 4088 4745 Size: 393 566 333 792 489 114 162 657 Cuts at: 4745 5942 6539 7326 7594 1239 Size: 1197 597 787 268 1524 Fragments arranged by size: 1524 1197 792 787 657 597 566 489 393 333 268 162 114 EcoNI CCTnn'n_nnAGG Cuts at: 2360 2459 2711 3140 3323 2360 Size: 99 252 429 183 6916 Fragments arranged by size: 6916 429 252 183 99 EcoO109I rG'GnC_Cy Cuts at: 2161 2248 2581 2816 3007 3145 3211 3316 3662 Size: 87 333 235 191 138 66 105 346 Cuts at: 3662 3871 4390 2161 Size: 209 519 5650 Fragments arranged by size: 5650 519 346 333 235 209 191 138 105 87 66 EcoRI G'AATT_C Cuts at: 4397 5188 5566 4397 Size: 791 378 6710 Fragments arranged by size: 6710 791 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2926 2926 Size: 7879 FseI GG_CCGG'CC Cuts at: 3040 3040 Size: 7879 FspI TGC'GCA Cuts at: 6385 6385 Size: 7879 HaeII r_GCGC'y Cuts at: 1678 3346 4223 4455 5105 7026 1678 Size: 1668 877 232 650 1921 2531 Fragments arranged by size: 2531 1921 1668 877 650 232 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2327 2359 2732 2764 2862 Size: 32 135 32 755 32 373 32 98 Cuts at: 2862 2894 3489 3521 4330 4362 5157 5189 6520 Size: 32 595 32 809 32 795 32 1331 Cuts at: 6520 6552 6594 6626 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1331 809 795 755 595 373 135 98 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3994 4273 5223 5469 669 Size: 988 281 2056 279 950 246 3079 Fragments arranged by size: 3079 2056 988 950 281 279 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7879 HpaI GTT'AAC Cuts at: 5469 5469 Size: 7879 KpnI G_GTAC'C Cuts at: 5204 5204 Size: 7879 MfeI C'AATT_G Cuts at: 5478 5478 Size: 7879 MluI A'CGCG_T Cuts at: 7755 7755 Size: 7879 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2871 2880 4626 5016 6993 7177 1305 Size: 1566 9 1746 390 1977 184 2007 Fragments arranged by size: 2007 1977 1746 1566 390 184 9 MscI TGG'CCA Cuts at: 10 64 1489 1832 2129 3719 4367 10 Size: 54 1425 343 297 1590 648 3522 Fragments arranged by size: 3522 1590 1425 648 343 297 54 MslI CAynn'nnrTG Cuts at: 518 1166 2547 3752 5837 6196 6355 518 Size: 648 1381 1205 2085 359 159 2042 Fragments arranged by size: 2085 2042 1381 1205 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2020 2179 2521 2989 3608 3765 4177 5015 Size: 414 159 342 468 619 157 412 838 Cuts at: 5015 5295 5972 7120 7365 1606 Size: 280 677 1148 245 2120 Fragments arranged by size: 2120 1148 838 677 619 468 414 412 342 280 245 159 157 NaeI GCC'GGC Cuts at: 3038 3038 Size: 7879 NcoI C'CATG_G Cuts at: 513 1065 2796 513 Size: 552 1731 5596 Fragments arranged by size: 5596 1731 552 NdeI CA'TA_TG Cuts at: 387 3166 4578 387 Size: 2779 1412 3688 Fragments arranged by size: 3688 2779 1412 NgoMIV G'CCGG_C Cuts at: 3036 3036 Size: 7879 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7879 NotI GC'GGCC_GC Cuts at: 5326 5326 Size: 7879 NspI r_CATG'y Cuts at: 1132 1229 3751 5237 6782 1132 Size: 97 2522 1486 1545 2229 Fragments arranged by size: 2522 2229 1545 1486 97 PasI CC'CwG_GG Cuts at: 1348 3567 4978 1348 Size: 2219 1411 4249 Fragments arranged by size: 4249 2219 1411 PciI A'CATG_T Cuts at: 6778 6778 Size: 7879 PflMI CCAn_nnn'nTGG Cuts at: 1108 2488 1108 Size: 1380 6499 PfoI T'CCnGG_A Cuts at: 3245 3245 Size: 7879 PmeI GTTT'AAAC Cuts at: 5183 5183 Size: 7879 PmlI CAC'GTG Cuts at: 5095 5095 Size: 7879 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5948 5980 7461 7493 5948 Size: 32 1481 32 6334 Fragments arranged by size: 6334 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2816 3145 3316 3662 3871 4390 2816 Size: 329 171 346 209 519 6305 Fragments arranged by size: 6305 519 346 329 209 171 PshAI GACnn'nnGTC Cuts at: 1613 4476 1613 Size: 2863 5016 PsiI TTA'TAA Cuts at: 5449 5449 Size: 7879 PspOMI G'GGCC_C Cuts at: 4235 4235 Size: 7879 PspXI vC'TCGA_Gb Cuts at: 3105 3105 Size: 7879 PstI C_TGCA'G Cuts at: 830 3607 3942 4098 4380 4449 4639 4896 5231 Size: 2777 335 156 282 69 190 257 335 Cuts at: 5231 830 Size: 3478 Fragments arranged by size: 3478 2777 335 335 282 257 190 156 69 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7879 PvuII CAG'CTG Cuts at: 2179 2521 2989 3608 4177 5015 2179 Size: 342 468 619 569 838 5043 Fragments arranged by size: 5043 838 619 569 468 342 SacI G_AGCT'C Cuts at: 2418 4491 2418 Size: 2073 5806 SalI G'TCGA_C Cuts at: 1936 5221 1936 Size: 3285 4594 SapI GCTCTTCn'nnn_ Cuts at: 2603 2603 Size: 7879 SbfI CC_TGCA'GG Cuts at: 5231 5231 Size: 7879 ScaI AGT'ACT Cuts at: 4157 4844 6127 7839 4157 Size: 687 1283 1712 4197 Fragments arranged by size: 4197 1712 1283 687 SfcI C'TryA_G Cuts at: 826 1045 3603 3938 4094 4376 4445 4635 4892 Size: 219 2558 335 156 282 69 190 257 Cuts at: 4892 5227 6362 7043 7234 826 Size: 335 1135 681 191 1471 Fragments arranged by size: 2558 1471 1135 681 335 335 282 257 219 191 190 156 69 SfiI GGCCn_nnn'nGGCC Cuts at: 2247 3046 2247 Size: 799 7080 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7879 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7879 SmaI CCC'GGG Cuts at: 1872 5206 1872 Size: 3334 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3105 3290 3455 5024 5111 5983 Size: 197 926 1162 185 165 1569 87 872 Cuts at: 5983 6884 7146 820 Size: 901 262 1553 Fragments arranged by size: 1569 1553 1162 926 901 872 262 197 185 165 87 SnaBI TAC'GTA Cuts at: 493 5670 493 Size: 5177 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7879 SphI G_CATG'C Cuts at: 1132 5237 1132 Size: 4105 3774 SspI AAT'ATT Cuts at: 5 52 5803 5 Size: 47 5751 2081 Fragments arranged by size: 5751 2081 47 StuI AGG'CCT Cuts at: 1517 2241 3239 3966 1517 Size: 724 998 727 5430 Fragments arranged by size: 5430 998 727 724 StyI C'CwwG_G Cuts at: 513 1065 2281 2679 2796 2919 4056 5318 513 Size: 552 1216 398 117 123 1137 1262 3074 Fragments arranged by size: 3074 1262 1216 1137 552 398 123 117 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2519 3205 3416 4166 4368 5906 5923 Size: 1255 939 686 211 750 202 1538 17 Cuts at: 5923 6076 6261 6706 325 Size: 153 185 445 1498 Fragments arranged by size: 1538 1498 1255 939 750 686 445 211 202 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2761 3750 4155 Size: 274 80 33 51 1443 784 989 405 Cuts at: 4155 4731 4842 6125 7837 96 Size: 576 111 1283 1712 138 Fragments arranged by size: 1712 1443 1283 989 784 576 405 274 138 111 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7839 7871 7839 Size: 32 7847 Tth111I GACn'n_nGTC Cuts at: 1316 2744 3850 1316 Size: 1428 1106 5345 Fragments arranged by size: 5345 1428 1106 XbaI T'CTAG_A Cuts at: 4480 5215 4480 Size: 735 7144 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2506 2993 3475 3707 4702 2506 Size: 487 482 232 995 5683 Fragments arranged by size: 5683 995 487 482 232 XhoI C'TCGA_G Cuts at: 1943 3105 1943 Size: 1162 6717 XmaI C'CCGG_G Cuts at: 1870 5204 1870 Size: 3334 4545 XmnI GAAnn'nnTTC Cuts at: 3416 3911 4778 6008 3416 Size: 495 867 1230 5287 Fragments arranged by size: 5287 1230 867 495 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5687 276 Size: 53 83 186 936 4153 2468 Fragments arranged by size: 4153 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I EcoRV FspAI KasI NarI NruI NsiI PacI PsrI RsrII SacII SanDI SexAI SfoI SrfI SwaI