(Circular) (Six-base) MAPSORT of: pFN21AB4115.seq Check: 9903 from: 1 to: 7519 pFN21AB4115 7519 bp With 182 enzymes: SgfI * October 20, 2009 10:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5329 278 Size: 53 83 186 936 3793 2468 Fragments arranged by size: 3793 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3160 4862 1937 Size: 1223 1702 4594 Fragments arranged by size: 4594 1702 1223 Acc65I G'GTAC_C Cuts at: 4840 4840 Size: 7519 AclI AA'CG_TT Cuts at: 1563 2259 5646 6019 1563 Size: 696 3387 373 3063 Fragments arranged by size: 3387 3063 696 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2388 3107 3480 3558 4362 5582 6966 7234 Size: 756 719 373 78 804 1220 1384 268 Cuts at: 7234 1632 Size: 1917 Fragments arranged by size: 1917 1384 1220 804 756 719 373 268 78 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7322 AflIII A'CryG_T Cuts at: 1203 3871 6418 7395 1203 Size: 2668 2547 977 1327 Fragments arranged by size: 2668 2547 1327 977 AgeI A'CCGG_T Cuts at: 6350 6350 Size: 7519 AhdI GACnn_n'nnGTC Cuts at: 6248 6248 Size: 7519 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7519 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3048 1184 Size: 1864 5655 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2079 2111 2079 Size: 32 7487 AlwNI CAG_nnn'CTG Cuts at: 5262 6834 5262 Size: 1572 5947 ApaLI G'TGCA_C Cuts at: 5576 6732 5576 Size: 1156 6363 ApoI r'AATT_y Cuts at: 1493 3485 4216 4828 5054 5206 7315 1493 Size: 1992 731 612 226 152 2109 1697 Fragments arranged by size: 2109 1992 1697 731 612 226 152 AseI AT'TA_AT Cuts at: 160 1032 6073 160 Size: 872 5041 1606 Fragments arranged by size: 5041 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7519 AvaI C'yCGr_G Cuts at: 1870 1943 4844 1870 Size: 73 2901 4545 Fragments arranged by size: 4545 2901 73 AvrII C'CTAG_G Cuts at: 3976 3976 Size: 7519 BamHI G'GATC_C Cuts at: 4849 5216 4849 Size: 367 7152 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4325 4840 6295 618 Size: 325 217 627 2538 515 1455 1842 Fragments arranged by size: 2538 1842 1455 627 515 325 217 BanII G_rGCy'C Cuts at: 1391 4007 4357 4698 4838 1391 Size: 2616 350 341 140 4072 Fragments arranged by size: 4072 2616 350 341 140 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2544 928 Size: 971 645 5903 Fragments arranged by size: 5903 971 645 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2789 2823 5710 5744 1903 Size: 34 852 34 2887 34 3678 Fragments arranged by size: 3678 2887 852 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4526 5411 6621 4526 Size: 885 1210 5424 Fragments arranged by size: 5424 1210 885 BclI T'GATC_A Cuts at: 1548 2614 1548 Size: 1066 6453 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2123 6130 136 Size: 107 122 71 631 605 451 4007 1525 Fragments arranged by size: 4007 1525 631 605 451 122 107 71 BglII A'GATC_T Cuts at: 2704 3892 7514 2704 Size: 1188 3622 2709 Fragments arranged by size: 3622 2709 1188 BlpI GC'TnA_GC Cuts at: 4936 4936 Size: 7519 Bme1580I G_kGCm'C Cuts at: 1790 4330 5580 6736 1790 Size: 2540 1250 1156 2573 Fragments arranged by size: 2573 2540 1250 1156 BmgBI CAC'GTC Cuts at: 1318 4502 1318 Size: 3184 4335 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3647 4413 5250 Size: 416 53 83 399 24 2230 766 837 Cuts at: 5250 6208 442 Size: 958 1753 Fragments arranged by size: 2230 1753 958 837 766 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3548 1056 Size: 2492 5027 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 6179 1239 Size: 4940 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5644 6509 6807 5644 Size: 865 298 6356 Fragments arranged by size: 6356 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2737 6182 882 Size: 617 1238 3445 2219 Fragments arranged by size: 3445 2219 1238 617 BsaAI yAC'GTr Cuts at: 493 1204 5310 493 Size: 711 4106 2702 Fragments arranged by size: 4106 2702 711 BsaBI GATnn'nnATC Cuts at: 2343 3600 4572 7513 2343 Size: 1257 972 2941 2349 Fragments arranged by size: 2941 2349 1257 972 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3475 5326 5708 6343 Size: 53 83 186 936 1942 1851 382 635 Cuts at: 6343 275 Size: 1451 Fragments arranged by size: 1942 1851 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3846 5952 6350 6624 6771 7182 3846 Size: 2106 398 274 147 411 4183 Fragments arranged by size: 4183 2106 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2079 2109 1178 Size: 30 165 30 676 30 6588 Fragments arranged by size: 6588 676 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3105 3500 3984 1186 Size: 1919 395 484 4721 Fragments arranged by size: 4721 1919 484 395 BseYI C'CCAG_C Cuts at: 2072 4181 6722 7218 2072 Size: 2109 2541 496 2373 Fragments arranged by size: 2541 2373 2109 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2727 3404 4224 1163 Size: 573 991 677 820 4458 Fragments arranged by size: 4458 991 820 677 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4969 5730 6758 1540 Size: 275 186 2968 761 1028 2301 Fragments arranged by size: 2968 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5580 5665 6736 5580 Size: 85 1071 6363 Fragments arranged by size: 6363 1071 85 BsmI GAATG_Cn' Cuts at: 2357 3913 5030 5123 2357 Size: 1556 1117 93 4753 Fragments arranged by size: 4753 1556 1117 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4007 4330 4357 4698 4838 5580 5665 Size: 399 2217 323 27 341 140 742 85 Cuts at: 5665 6736 1391 Size: 1071 2174 Fragments arranged by size: 2217 2174 1071 742 399 341 323 140 85 27 BspEI T'CCGG_A Cuts at: 3846 7182 3846 Size: 3336 4183 BspHI T'CATG_A Cuts at: 5408 7138 5408 Size: 1730 5789 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4874 844 Size: 4030 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3779 3991 5406 1394 Size: 429 1956 212 1415 3507 Fragments arranged by size: 3507 1956 1415 429 212 BsrDI GCAATG_nn' Cuts at: 65 1248 3906 4104 6014 6188 65 Size: 1183 2658 198 1910 174 1396 Fragments arranged by size: 2658 1910 1396 1183 198 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4611 6163 6350 1700 Size: 110 2801 1552 187 2869 Fragments arranged by size: 2869 2801 1552 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7519 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7519 BssSI C'ACGA_G Cuts at: 3732 5579 6591 3732 Size: 1847 1012 4660 Fragments arranged by size: 4660 1847 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7519 BstBI TT'CG_AA Cuts at: 6323 6323 Size: 7519 BstEII G'GTnAC_C Cuts at: 1184 3836 1184 Size: 2652 4867 BstXI CCAn_nnnn'nTGG Cuts at: 4529 4529 Size: 7519 BstYI r'GATC_y Cuts at: 1972 2192 2704 3027 3892 4849 5216 5601 5618 Size: 220 512 323 865 957 367 385 17 Cuts at: 5618 7070 7156 7168 7514 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 957 865 512 385 367 346 323 220 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3589 3682 4522 513 Size: 552 2524 93 840 3510 Fragments arranged by size: 3510 2524 840 552 93 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2352 487 Size: 683 56 1126 5654 Fragments arranged by size: 5654 1126 683 56 BtsI GCAGTG_nn' Cuts at: 2997 5035 5829 5849 2997 Size: 2038 794 20 4667 Fragments arranged by size: 4667 2038 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7519 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3655 3690 4303 4338 529 Size: 35 3091 35 613 35 3710 Fragments arranged by size: 3710 3091 613 35 35 35 DraI TTT'AAA Cuts at: 4823 4823 Size: 7519 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3653 4268 4342 6526 809 Size: 507 2337 615 74 2184 1802 Fragments arranged by size: 2337 2184 1802 615 507 74 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3078 3348 3663 Size: 54 1425 50 275 18 1248 270 315 Cuts at: 3663 3763 4570 4966 5855 7484 8 Size: 100 807 396 889 1629 43 Fragments arranged by size: 1629 1425 1248 889 807 396 315 275 270 100 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4966 1537 Size: 275 3154 4090 Fragments arranged by size: 4090 3154 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2140 2393 2454 2526 2607 2664 3499 3534 Size: 787 253 61 72 81 57 835 35 Cuts at: 3534 3769 5449 1353 Size: 235 1680 3423 Fragments arranged by size: 3423 1680 835 787 253 235 81 72 61 57 35 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6106 6480 6626 387 Size: 1064 138 4517 374 146 1280 Fragments arranged by size: 4517 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2388 3107 3480 3558 4362 5582 6179 Size: 393 756 719 373 78 804 1220 597 Cuts at: 6179 6966 7234 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1220 804 787 756 719 597 393 373 268 78 EcoNI CCTnn'n_nnAGG Cuts at: 2754 3125 3560 4068 2754 Size: 371 435 508 6205 Fragments arranged by size: 6205 508 435 371 EcoO109I rG'GnC_Cy Cuts at: 3118 3118 Size: 7519 EcoRI G'AATT_C Cuts at: 4216 4828 5206 4216 Size: 612 378 6529 Fragments arranged by size: 6529 612 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3225 4150 3225 Size: 925 6594 FspI TGC'GCA Cuts at: 6025 6025 Size: 7519 HaeII r_GCGC'y Cuts at: 1678 6666 1678 Size: 4988 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2617 2649 2986 3018 4465 Size: 32 135 32 1045 32 337 32 1447 Cuts at: 4465 4497 6160 6192 6234 6266 1373 Size: 32 1663 32 42 32 2626 Fragments arranged by size: 2626 1663 1447 1045 337 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3161 4366 4863 5109 669 Size: 988 281 1223 1205 497 246 3079 Fragments arranged by size: 3079 1223 1205 988 497 281 246 HindIII A'AGCT_T Cuts at: 748 2478 4668 748 Size: 1730 2190 3599 Fragments arranged by size: 3599 2190 1730 HpaI GTT'AAC Cuts at: 5109 5109 Size: 7519 KpnI G_GTAC'C Cuts at: 4844 4844 Size: 7519 MfeI C'AATT_G Cuts at: 2571 2985 5118 2571 Size: 414 2133 4972 Fragments arranged by size: 4972 2133 414 MluI A'CGCG_T Cuts at: 7395 7395 Size: 7519 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2946 4250 4625 6633 6817 1305 Size: 1641 1304 375 2008 184 2007 Fragments arranged by size: 2008 2007 1641 1304 375 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3350 3665 4572 10 Size: 54 1425 343 1518 315 907 2957 Fragments arranged by size: 2957 1518 1425 907 343 315 54 MslI CAynn'nnrTG Cuts at: 518 1166 4509 4527 4566 5477 5836 5995 518 Size: 648 3343 18 39 911 359 159 2042 Fragments arranged by size: 3343 2042 911 648 359 159 39 18 MspA1I CmG'CkG Cuts at: 1606 4763 4935 5612 6760 7005 1606 Size: 3157 172 677 1148 245 2120 Fragments arranged by size: 3157 2120 1148 677 245 172 NaeI GCC'GGC Cuts at: 4613 4613 Size: 7519 NcoI C'CATG_G Cuts at: 513 1065 3589 4522 513 Size: 552 2524 933 3510 Fragments arranged by size: 3510 2524 933 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7519 NgoMIV G'CCGG_C Cuts at: 4611 4611 Size: 7519 NheI G'CTAG_C Cuts at: 1052 3544 1052 Size: 2492 5027 NotI GC'GGCC_GC Cuts at: 4966 4966 Size: 7519 NspI r_CATG'y Cuts at: 1132 1229 4877 6422 1132 Size: 97 3648 1545 2229 Fragments arranged by size: 3648 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7519 PciI A'CATG_T Cuts at: 6418 6418 Size: 7519 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 2513 2978 4301 1108 Size: 902 503 465 1323 4326 Fragments arranged by size: 4326 1323 902 503 465 PmeI GTTT'AAAC Cuts at: 4823 4823 Size: 7519 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2079 2111 5588 5620 7101 7133 2079 Size: 32 3477 32 1481 32 2465 Fragments arranged by size: 3477 2465 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7519 PsiI TTA'TAA Cuts at: 5089 5089 Size: 7519 PstI C_TGCA'G Cuts at: 830 2920 4871 830 Size: 2090 1951 3478 Fragments arranged by size: 3478 2090 1951 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7519 PvuII CAG'CTG Cuts at: 4763 4763 Size: 7519 SalI G'TCGA_C Cuts at: 1936 3159 4861 1936 Size: 1223 1702 4594 Fragments arranged by size: 4594 1702 1223 SapI GCTCTTCn'nnn_ Cuts at: 2140 3534 3769 2140 Size: 1394 235 5890 Fragments arranged by size: 5890 1394 235 SbfI CC_TGCA'GG Cuts at: 4871 4871 Size: 7519 ScaI AGT'ACT Cuts at: 5767 7479 5767 Size: 1712 5807 SexAI A'CCwGG_T Cuts at: 2971 3840 2971 Size: 869 6650 SfcI C'TryA_G Cuts at: 826 1045 2916 3982 4867 6002 6683 6874 826 Size: 219 1871 1066 885 1135 681 191 1471 Fragments arranged by size: 1871 1471 1135 1066 885 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7519 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7519 SmaI CCC'GGG Cuts at: 1872 4846 1872 Size: 2974 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5623 6524 6786 820 Size: 197 926 3680 901 262 1553 Fragments arranged by size: 3680 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5310 493 Size: 4817 2702 SpeI A'CTAG_T Cuts at: 152 4199 152 Size: 4047 3472 SphI G_CATG'C Cuts at: 1132 4877 1132 Size: 3745 3774 SspI AAT'ATT Cuts at: 5 52 5443 5 Size: 47 5391 2081 Fragments arranged by size: 5391 2081 47 StuI AGG'CCT Cuts at: 1517 3975 4055 1517 Size: 2458 80 4981 Fragments arranged by size: 4981 2458 80 StyI C'CwwG_G Cuts at: 513 1065 2349 3114 3589 3816 3976 4284 4357 Size: 552 1284 765 475 227 160 308 73 Cuts at: 4357 4522 4958 513 Size: 165 436 3074 Fragments arranged by size: 3074 1284 765 552 475 436 308 227 165 160 73 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4182 5546 5563 5716 5901 6346 325 Size: 1255 2602 1364 17 153 185 445 1498 Fragments arranged by size: 2602 1498 1364 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4122 5765 7477 Size: 274 80 33 51 1443 2145 1643 1712 Cuts at: 7477 96 Size: 138 Fragments arranged by size: 2145 1712 1643 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2005 2037 7479 7511 2005 Size: 32 5442 32 2013 Fragments arranged by size: 5442 2013 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3842 1316 Size: 2526 4993 XbaI T'CTAG_A Cuts at: 2545 4855 2545 Size: 2310 5209 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2117 4075 2117 Size: 1958 5561 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7519 XmaI C'CCGG_G Cuts at: 1870 4844 1870 Size: 2974 4545 XmnI GAAnn'nnTTC Cuts at: 5648 5648 Size: 7519 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5327 276 Size: 53 83 186 936 3793 2468 Fragments arranged by size: 3793 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI