(Circular) (Six-base) MAPSORT of: pFN21AB4107.seq Check: 1905 from: 1 to: 6628 pFN21AB4107 6628 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3262 3262 Size: 6628 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4438 278 Size: 53 83 186 936 2902 2468 Fragments arranged by size: 2902 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3971 1937 Size: 2034 4594 Acc65I G'GTAC_C Cuts at: 3949 3949 Size: 6628 AclI AA'CG_TT Cuts at: 1563 4755 5128 1563 Size: 3192 373 3063 Fragments arranged by size: 3192 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3117 4691 6075 6343 1632 Size: 1485 1574 1384 268 1917 Fragments arranged by size: 1917 1574 1485 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6431 AflIII A'CryG_T Cuts at: 1203 2703 5527 6504 1203 Size: 1500 2824 977 1327 Fragments arranged by size: 2824 1500 1327 977 AgeI A'CCGG_T Cuts at: 5459 5459 Size: 6628 AhdI GACnn_n'nnGTC Cuts at: 5357 5357 Size: 6628 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6628 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6628 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2556 2588 2556 Size: 32 6596 AlwNI CAG_nnn'CTG Cuts at: 4371 5943 4371 Size: 1572 5056 ApaI G_GGCC'C Cuts at: 3363 3363 Size: 6628 ApaLI G'TGCA_C Cuts at: 2870 4685 5841 2870 Size: 1815 1156 3657 Fragments arranged by size: 3657 1815 1156 ApoI r'AATT_y Cuts at: 1493 2042 2864 3056 3937 4163 4315 6424 1493 Size: 549 822 192 881 226 152 2109 1697 Fragments arranged by size: 2109 1697 881 822 549 226 192 152 AseI AT'TA_AT Cuts at: 160 1032 5182 160 Size: 872 4150 1606 Fragments arranged by size: 4150 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6628 AvaI C'yCGr_G Cuts at: 1870 1943 2535 3953 1870 Size: 73 592 1418 4545 Fragments arranged by size: 4545 1418 592 73 BamHI G'GATC_C Cuts at: 2149 3958 4325 2149 Size: 1809 367 4452 Fragments arranged by size: 4452 1809 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2483 3538 3949 5404 618 Size: 325 217 627 696 1055 411 1455 1842 Fragments arranged by size: 1842 1455 1055 696 627 411 325 217 BanII G_rGCy'C Cuts at: 1391 2362 3319 3363 3947 1391 Size: 971 957 44 584 4072 Fragments arranged by size: 4072 971 957 584 44 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2706 928 Size: 971 807 4850 Fragments arranged by size: 4850 971 807 BbvCI CC'TCA_GC Cuts at: 2515 2515 Size: 6628 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4819 4853 1903 Size: 34 2882 34 3678 Fragments arranged by size: 3678 2882 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4520 5730 4520 Size: 1210 5418 BclI T'GATC_A Cuts at: 1548 1548 Size: 6628 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3277 5239 136 Size: 107 122 71 631 605 1605 1962 1525 Fragments arranged by size: 1962 1605 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2015 2674 6623 2015 Size: 659 3949 2020 Fragments arranged by size: 3949 2020 659 BlpI GC'TnA_GC Cuts at: 2397 4045 2397 Size: 1648 4980 Bme1580I G_kGCm'C Cuts at: 1790 2488 2874 3363 3613 3744 4689 5845 1790 Size: 698 386 489 250 131 945 1156 2573 Fragments arranged by size: 2573 1156 945 698 489 386 250 131 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6628 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2198 4359 5317 Size: 416 53 83 399 24 781 2161 958 Cuts at: 5317 442 Size: 1753 Fragments arranged by size: 2161 1753 958 781 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6628 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3294 3366 3414 3477 5288 1239 Size: 2055 72 48 63 1811 2579 Fragments arranged by size: 2579 2055 1811 72 63 48 Bpu10I CC'TnA_GC Cuts at: 2515 2515 Size: 6628 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2616 4753 5618 5916 2616 Size: 2137 865 298 3328 Fragments arranged by size: 3328 2137 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3325 5291 882 Size: 617 1826 1966 2219 Fragments arranged by size: 2219 1966 1826 617 BsaAI yAC'GTr Cuts at: 493 1204 4419 493 Size: 711 3215 2702 Fragments arranged by size: 3215 2702 711 BsaBI GATnn'nnATC Cuts at: 2020 2775 6622 2020 Size: 755 3847 2026 Fragments arranged by size: 3847 2026 755 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4435 4817 5452 275 Size: 53 83 186 936 2902 382 635 1451 Fragments arranged by size: 2902 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5061 5459 5733 5880 6291 5061 Size: 398 274 147 411 5398 Fragments arranged by size: 5398 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3772 3802 1178 Size: 30 165 30 2369 30 4004 Fragments arranged by size: 4004 2369 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2616 2619 2979 3050 3309 3444 1186 Size: 1430 3 360 71 259 135 4370 Fragments arranged by size: 4370 1430 360 259 135 71 3 BseYI C'CCAG_C Cuts at: 2487 2727 5831 6327 2487 Size: 240 3104 496 2788 Fragments arranged by size: 3104 2788 496 240 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2660 3471 3675 3732 1163 Size: 573 924 811 204 57 4059 Fragments arranged by size: 4059 924 811 573 204 57 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4078 4839 5867 1540 Size: 275 186 2077 761 1028 2301 Fragments arranged by size: 2301 2077 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2874 3319 4689 4774 5845 2874 Size: 445 1370 85 1071 3657 Fragments arranged by size: 3657 1370 1071 445 85 BsmI GAATG_Cn' Cuts at: 2398 3348 4139 4232 2398 Size: 950 791 93 4794 Fragments arranged by size: 4794 950 791 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2362 2488 2874 3319 3363 3613 3744 Size: 399 572 126 386 445 44 250 131 Cuts at: 3744 3947 4689 4774 5845 1391 Size: 203 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 572 445 399 386 250 203 131 126 85 44 BspEI T'CCGG_A Cuts at: 6291 6291 Size: 6628 BspHI T'CATG_A Cuts at: 2737 3903 4517 6247 2737 Size: 1166 614 1730 3118 Fragments arranged by size: 3118 1730 1166 614 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3008 3262 3562 3983 844 Size: 2164 254 300 421 3489 Fragments arranged by size: 3489 2164 421 300 254 BsrBI CCG'CTC Cuts at: 1394 1823 4515 1394 Size: 429 2692 3507 Fragments arranged by size: 3507 2692 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5123 5297 65 Size: 1183 3875 174 1396 Fragments arranged by size: 3875 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5272 5459 1700 Size: 110 3462 187 2869 Fragments arranged by size: 3462 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6628 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6628 BssSI C'ACGA_G Cuts at: 3456 4688 5700 3456 Size: 1232 1012 4384 Fragments arranged by size: 4384 1232 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6628 BstBI TT'CG_AA Cuts at: 5432 5432 Size: 6628 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6628 BstXI CCAn_nnnn'nTGG Cuts at: 3146 3146 Size: 6628 BstYI r'GATC_y Cuts at: 1972 2015 2149 2674 3958 4325 4710 4727 6179 Size: 43 134 525 1284 367 385 17 1452 Cuts at: 6179 6265 6277 6623 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1284 525 385 367 346 134 86 43 17 12 Bsu36I CC'TnA_GG Cuts at: 2781 3033 2781 Size: 252 6376 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5137 Fragments arranged by size: 5137 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5889 Fragments arranged by size: 5889 683 56 BtsI GCAGTG_nn' Cuts at: 3156 3744 4144 4938 4958 3156 Size: 588 400 794 20 4826 Fragments arranged by size: 4826 794 588 400 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6628 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3603 3638 529 Size: 35 3039 35 3519 Fragments arranged by size: 3519 3039 35 35 DraI TTT'AAA Cuts at: 2139 3932 2139 Size: 1793 4835 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5635 809 Size: 507 4319 1802 Fragments arranged by size: 4319 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2650 4075 4964 Size: 54 1425 50 275 18 820 1425 889 Cuts at: 4964 6593 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1425 889 820 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4075 1537 Size: 275 2263 4090 Fragments arranged by size: 4090 2263 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2078 2348 2562 2997 4558 1353 Size: 725 270 214 435 1561 3423 Fragments arranged by size: 3423 1561 725 435 270 214 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3171 3656 5215 5589 5735 387 Size: 1064 138 1582 485 1559 374 146 1280 Fragments arranged by size: 1582 1559 1280 1064 485 374 146 138 EcoICRI GAG'CTC Cuts at: 3317 3317 Size: 6628 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3117 3294 3366 3414 3477 4691 5288 Size: 393 1485 177 72 48 63 1214 597 Cuts at: 5288 6075 6343 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1485 1214 787 597 393 268 177 72 63 48 EcoNI CCTnn'n_nnAGG Cuts at: 3417 3417 Size: 6628 EcoO109I rG'GnC_Cy Cuts at: 3359 3360 3359 Size: 1 6627 EcoRI G'AATT_C Cuts at: 3937 4315 3937 Size: 378 6250 FspI TGC'GCA Cuts at: 5134 5134 Size: 6628 HaeII r_GCGC'y Cuts at: 1678 5775 1678 Size: 4097 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2134 2166 3137 3169 3697 Size: 32 135 32 562 32 971 32 528 Cuts at: 3697 3729 3745 3777 5269 5301 5343 5375 1373 Size: 32 16 32 1492 32 42 32 2626 Fragments arranged by size: 2626 1492 971 562 528 135 42 32 32 32 32 32 32 32 32 16 HincII GTy'rAC Cuts at: 669 1657 1938 3972 4218 669 Size: 988 281 2034 246 3079 Fragments arranged by size: 3079 2034 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6628 HpaI GTT'AAC Cuts at: 4218 4218 Size: 6628 KpnI G_GTAC'C Cuts at: 3953 3953 Size: 6628 MfeI C'AATT_G Cuts at: 4227 4227 Size: 6628 MluI A'CGCG_T Cuts at: 6504 6504 Size: 6628 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2475 2555 5742 5926 1305 Size: 1170 80 3187 184 2007 Fragments arranged by size: 3187 2007 1170 184 80 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4806 Fragments arranged by size: 4806 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2742 3144 4586 4945 5104 518 Size: 648 1576 402 1442 359 159 2042 Fragments arranged by size: 2042 1576 1442 648 402 359 159 MspA1I CmG'CkG Cuts at: 1606 4044 4721 5869 6114 1606 Size: 2438 677 1148 245 2120 Fragments arranged by size: 2438 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5137 Fragments arranged by size: 5137 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6628 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6628 NotI GC'GGCC_GC Cuts at: 4075 4075 Size: 6628 NspI r_CATG'y Cuts at: 1132 1229 2405 2444 2707 3986 5531 1132 Size: 97 1176 39 263 1279 1545 2229 Fragments arranged by size: 2229 1545 1279 1176 263 97 39 PasI CC'CwG_GG Cuts at: 1348 3563 3596 1348 Size: 2215 33 4380 Fragments arranged by size: 4380 2215 33 PciI A'CATG_T Cuts at: 2703 5527 2703 Size: 2824 3804 PflMI CCAn_nnn'nTGG Cuts at: 1108 2480 3242 3596 1108 Size: 1372 762 354 4140 Fragments arranged by size: 4140 1372 762 354 PfoI T'CCnGG_A Cuts at: 2463 3310 3382 2463 Size: 847 72 5709 Fragments arranged by size: 5709 847 72 PmeI GTTT'AAAC Cuts at: 3932 3932 Size: 6628 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4697 4729 6210 6242 4697 Size: 32 1481 32 5083 Fragments arranged by size: 5083 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6628 PsiI TTA'TAA Cuts at: 4198 4198 Size: 6628 PspOMI G'GGCC_C Cuts at: 3359 3359 Size: 6628 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2265 2297 2265 Size: 32 6596 PstI C_TGCA'G Cuts at: 830 2134 3980 830 Size: 1304 1846 3478 Fragments arranged by size: 3478 1846 1304 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6628 SacI G_AGCT'C Cuts at: 3319 3319 Size: 6628 SalI G'TCGA_C Cuts at: 1936 3970 1936 Size: 2034 4594 SbfI CC_TGCA'GG Cuts at: 3980 3980 Size: 6628 ScaI AGT'ACT Cuts at: 2235 2815 4876 6588 2235 Size: 580 2061 1712 2275 Fragments arranged by size: 2275 2061 1712 580 SfcI C'TryA_G Cuts at: 826 1045 2130 3285 3976 5111 5792 5983 826 Size: 219 1085 1155 691 1135 681 191 1471 Fragments arranged by size: 1471 1155 1135 1085 691 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3277 3277 Size: 6628 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6628 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6628 SmaI CCC'GGG Cuts at: 1872 3955 1872 Size: 2083 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2631 4732 5633 5895 820 Size: 197 926 688 2101 901 262 1553 Fragments arranged by size: 2101 1553 926 901 688 262 197 SnaBI TAC'GTA Cuts at: 493 4419 493 Size: 3926 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6628 SphI G_CATG'C Cuts at: 1132 3986 1132 Size: 2854 3774 SspI AAT'ATT Cuts at: 5 52 2375 4552 5 Size: 47 2323 2177 2081 Fragments arranged by size: 2323 2177 2081 47 StuI AGG'CCT Cuts at: 1517 3291 1517 Size: 1774 4854 StyI C'CwwG_G Cuts at: 513 1065 2004 3470 3547 3636 3717 4067 513 Size: 552 939 1466 77 89 81 350 3074 Fragments arranged by size: 3074 1466 939 552 350 89 81 77 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3631 3856 4655 4672 4825 5010 5455 Size: 1255 2051 225 799 17 153 185 445 Cuts at: 5455 325 Size: 1498 Fragments arranged by size: 2051 1498 1255 799 445 225 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2233 2813 3871 Size: 274 80 33 51 1443 256 580 1058 Cuts at: 3871 4874 6586 96 Size: 1003 1712 138 Fragments arranged by size: 1712 1443 1058 1003 580 274 256 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2484 2516 6588 6620 2484 Size: 32 4072 32 2492 Fragments arranged by size: 4072 2492 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6628 XbaI T'CTAG_A Cuts at: 3964 3964 Size: 6628 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6628 XmaI C'CCGG_G Cuts at: 1870 3953 1870 Size: 2083 4545 XmnI GAAnn'nnTTC Cuts at: 2277 4757 2277 Size: 2480 4148 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4436 276 Size: 53 83 186 936 2902 2468 Fragments arranged by size: 2902 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI SacI SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PvuII RsrII SacII SanDI SapI SexAI SfoI SrfI SwaI XcmI