(Circular) (Six-base) MAPSORT of: pFN21AB4041.seq Check: 2329 from: 1 to: 6094 pFN21AB4041 6094 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3904 278 Size: 53 83 186 936 2368 2468 Fragments arranged by size: 2468 2368 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3437 1937 Size: 1500 4594 Acc65I G'GTAC_C Cuts at: 3415 3415 Size: 6094 AclI AA'CG_TT Cuts at: 1563 4221 4594 1563 Size: 2658 373 3063 Fragments arranged by size: 3063 2658 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4157 5541 5809 1632 Size: 2525 1384 268 1917 Fragments arranged by size: 2525 1917 1384 268 AfeI AGC'GCT Cuts at: 2285 2285 Size: 6094 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5897 AflIII A'CryG_T Cuts at: 1203 4993 5970 1203 Size: 3790 977 1327 Fragments arranged by size: 3790 1327 977 AgeI A'CCGG_T Cuts at: 2969 4925 2969 Size: 1956 4138 AhdI GACnn_n'nnGTC Cuts at: 4823 4823 Size: 6094 AleI CACnn'nnGTG Cuts at: 1166 2886 2970 3390 1166 Size: 1720 84 420 3870 Fragments arranged by size: 3870 1720 420 84 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6094 AlwNI CAG_nnn'CTG Cuts at: 3837 5409 3837 Size: 1572 4522 ApaLI G'TGCA_C Cuts at: 3073 4151 5307 3073 Size: 1078 1156 3860 Fragments arranged by size: 3860 1156 1078 ApoI r'AATT_y Cuts at: 1493 2215 2389 2541 2793 2877 3213 3403 3629 Size: 722 174 152 252 84 336 190 226 Cuts at: 3629 3781 5890 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 722 336 252 226 190 174 152 152 84 AseI AT'TA_AT Cuts at: 160 1032 4648 160 Size: 872 3616 1606 Fragments arranged by size: 3616 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6094 AvaI C'yCGr_G Cuts at: 1870 1943 2931 3419 1870 Size: 73 988 488 4545 Fragments arranged by size: 4545 988 488 73 BamHI G'GATC_C Cuts at: 3381 3424 3791 3381 Size: 43 367 5684 Fragments arranged by size: 5684 367 43 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2351 3415 4870 618 Size: 325 217 627 564 1064 1455 1842 Fragments arranged by size: 1842 1455 1064 627 564 325 217 BanII G_rGCy'C Cuts at: 1391 2603 2694 3413 1391 Size: 1212 91 719 4072 Fragments arranged by size: 4072 1212 719 91 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2759 928 Size: 971 860 4263 Fragments arranged by size: 4263 971 860 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4285 4319 1903 Size: 34 2348 34 3678 Fragments arranged by size: 3678 2348 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3039 3986 5196 3039 Size: 947 1210 3937 Fragments arranged by size: 3937 1210 947 BclI T'GATC_A Cuts at: 1548 2313 1548 Size: 765 5329 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4705 136 Size: 107 122 71 631 605 3033 1525 Fragments arranged by size: 3033 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6089 6089 Size: 6094 BlpI GC'TnA_GC Cuts at: 3511 3511 Size: 6094 Bme1580I G_kGCm'C Cuts at: 1790 3077 4155 5311 1790 Size: 1287 1078 1156 2573 Fragments arranged by size: 2573 1287 1156 1078 BmgBI CAC'GTC Cuts at: 1318 2104 1318 Size: 786 5308 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2107 2559 2727 Size: 416 53 83 399 24 690 452 168 Cuts at: 2727 3825 4783 442 Size: 1098 958 1753 Fragments arranged by size: 1753 1098 958 690 452 416 399 168 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6094 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2132 2272 4754 1239 Size: 893 140 2482 2579 Fragments arranged by size: 2579 2482 893 140 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2587 4219 5084 5382 2587 Size: 1632 865 298 3299 Fragments arranged by size: 3299 1632 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2137 4757 882 Size: 617 638 2620 2219 Fragments arranged by size: 2620 2219 638 617 BsaAI yAC'GTr Cuts at: 493 1204 3885 493 Size: 711 2681 2702 Fragments arranged by size: 2702 2681 711 BsaBI GATnn'nnATC Cuts at: 2315 6088 2315 Size: 3773 2321 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3901 4283 4918 275 Size: 53 83 186 936 2368 382 635 1451 Fragments arranged by size: 2368 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2969 4527 4925 5199 5346 5757 2969 Size: 1558 398 274 147 411 3306 Fragments arranged by size: 3306 1558 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5869 Fragments arranged by size: 5869 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2072 1186 Size: 886 5208 BseYI C'CCAG_C Cuts at: 3015 5297 5793 3015 Size: 2282 496 3316 Fragments arranged by size: 3316 2282 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5521 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3544 4305 5333 1540 Size: 275 186 1543 761 1028 2301 Fragments arranged by size: 2301 1543 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2694 3077 4155 4240 5311 2694 Size: 383 1078 85 1071 3477 Fragments arranged by size: 3477 1078 1071 383 85 BsmI GAATG_Cn' Cuts at: 2741 3605 3698 2741 Size: 864 93 5137 Fragments arranged by size: 5137 864 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2603 2694 3077 3413 4155 4240 5311 Size: 399 813 91 383 336 742 85 1071 Cuts at: 5311 1391 Size: 2174 Fragments arranged by size: 2174 1071 813 742 399 383 336 91 85 BspEI T'CCGG_A Cuts at: 5757 5757 Size: 6094 BspHI T'CATG_A Cuts at: 2191 3983 5713 2191 Size: 1792 1730 2572 Fragments arranged by size: 2572 1792 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2344 3449 844 Size: 1500 1105 3489 Fragments arranged by size: 3489 1500 1105 BsrBI CCG'CTC Cuts at: 1394 1823 3981 1394 Size: 429 2158 3507 Fragments arranged by size: 3507 2158 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4589 4763 65 Size: 1183 3341 174 1396 Fragments arranged by size: 3341 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2969 4738 4925 1700 Size: 110 1159 1769 187 2869 Fragments arranged by size: 2869 1769 1159 187 110 BsrGI T'GTAC_A Cuts at: 96 3242 96 Size: 3146 2948 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6094 BssSI C'ACGA_G Cuts at: 3038 3122 3197 4154 5166 3038 Size: 84 75 957 1012 3966 Fragments arranged by size: 3966 1012 957 84 75 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6094 BstBI TT'CG_AA Cuts at: 4898 4898 Size: 6094 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6094 BstYI r'GATC_y Cuts at: 1972 2961 3381 3424 3791 4176 4193 5645 5731 Size: 989 420 43 367 385 17 1452 86 Cuts at: 5731 5743 6089 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 989 420 385 367 346 86 43 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4603 Fragments arranged by size: 4603 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5355 Fragments arranged by size: 5355 683 56 BtsI GCAGTG_nn' Cuts at: 3610 4404 4424 3610 Size: 794 20 5280 Fragments arranged by size: 5280 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6094 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2728 2763 529 Size: 35 2164 35 3860 Fragments arranged by size: 3860 2164 35 35 DraI TTT'AAA Cuts at: 3398 3398 Size: 6094 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5101 809 Size: 507 3785 1802 Fragments arranged by size: 3785 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2242 3271 3541 Size: 54 1425 50 275 18 412 1029 270 Cuts at: 3541 4430 6059 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1029 889 412 275 270 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3541 1537 Size: 275 1729 4090 Fragments arranged by size: 4090 1729 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3251 4024 1353 Size: 1898 773 3423 Fragments arranged by size: 3423 1898 773 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4681 5055 5201 387 Size: 1064 138 3092 374 146 1280 Fragments arranged by size: 3092 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2692 2692 Size: 6094 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2132 2272 4157 4754 5541 5809 1239 Size: 393 500 140 1885 597 787 268 1524 Fragments arranged by size: 1885 1524 787 597 500 393 268 140 EcoO109I rG'GnC_Cy Cuts at: 2089 2089 Size: 6094 EcoRI G'AATT_C Cuts at: 2215 2389 2793 2877 3213 3403 3781 2215 Size: 174 404 84 336 190 378 4528 Fragments arranged by size: 4528 404 378 336 190 174 84 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2285 2521 2285 Size: 236 5858 FspI TGC'GCA Cuts at: 4600 4600 Size: 6094 HaeII r_GCGC'y Cuts at: 1678 2287 5241 1678 Size: 609 2954 2531 Fragments arranged by size: 2954 2531 609 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2132 2164 3180 3212 3250 Size: 32 135 32 560 32 1016 32 38 Cuts at: 3250 3282 4735 4767 4809 4841 1373 Size: 32 1453 32 42 32 2626 Fragments arranged by size: 2626 1453 1016 560 135 42 38 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2447 3438 3684 669 Size: 988 281 509 991 246 3079 Fragments arranged by size: 3079 991 988 509 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6094 HpaI GTT'AAC Cuts at: 3684 3684 Size: 6094 KpnI G_GTAC'C Cuts at: 3419 3419 Size: 6094 MfeI C'AATT_G Cuts at: 3097 3693 3097 Size: 596 5498 MluI A'CGCG_T Cuts at: 5970 5970 Size: 6094 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5208 5392 1305 Size: 3903 184 2007 Fragments arranged by size: 3903 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4272 Fragments arranged by size: 4272 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2466 2634 2886 2970 3390 4052 4411 Size: 648 1300 168 252 84 420 662 359 Cuts at: 4411 4570 518 Size: 159 2042 Fragments arranged by size: 2042 1300 662 648 420 359 252 168 159 84 MspA1I CmG'CkG Cuts at: 1606 3510 4187 5335 5580 1606 Size: 1904 677 1148 245 2120 Fragments arranged by size: 2120 1904 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4603 Fragments arranged by size: 4603 939 552 NdeI CA'TA_TG Cuts at: 387 2155 387 Size: 1768 4326 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6094 NotI GC'GGCC_GC Cuts at: 3541 3541 Size: 6094 NspI r_CATG'y Cuts at: 1132 1229 3348 3452 4997 1132 Size: 97 2119 104 1545 2229 Fragments arranged by size: 2229 2119 1545 104 97 PasI CC'CwG_GG Cuts at: 1348 2011 1348 Size: 663 5431 PciI A'CATG_T Cuts at: 4993 4993 Size: 6094 PflMI CCAn_nnn'nTGG Cuts at: 1108 2017 1108 Size: 909 5185 PmeI GTTT'AAAC Cuts at: 3398 3398 Size: 6094 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4163 4195 5676 5708 4163 Size: 32 1481 32 4549 Fragments arranged by size: 4549 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2089 2089 Size: 6094 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6094 PsiI TTA'TAA Cuts at: 3664 3664 Size: 6094 PspXI vC'TCGA_Gb Cuts at: 2931 2931 Size: 6094 PstI C_TGCA'G Cuts at: 830 3446 830 Size: 2616 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6094 SacI G_AGCT'C Cuts at: 2694 2694 Size: 6094 SalI G'TCGA_C Cuts at: 1936 3436 1936 Size: 1500 4594 SapI GCTCTTCn'nnn_ Cuts at: 3251 3251 Size: 6094 SbfI CC_TGCA'GG Cuts at: 3446 3446 Size: 6094 ScaI AGT'ACT Cuts at: 4342 6054 4342 Size: 1712 4382 SfcI C'TryA_G Cuts at: 826 1045 2119 2175 3442 4577 5258 5449 826 Size: 219 1074 56 1267 1135 681 191 1471 Fragments arranged by size: 1471 1267 1135 1074 681 219 191 56 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6094 SgrAI Cr'CCGG_yG Cuts at: 1700 2969 1700 Size: 1269 4825 SmaI CCC'GGG Cuts at: 1872 3421 1872 Size: 1549 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2602 2931 4198 5099 5361 820 Size: 197 926 659 329 1267 901 262 1553 Fragments arranged by size: 1553 1267 926 901 659 329 262 197 SnaBI TAC'GTA Cuts at: 493 3885 493 Size: 3392 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6094 SphI G_CATG'C Cuts at: 1132 3452 1132 Size: 2320 3774 SspI AAT'ATT Cuts at: 5 52 4018 5 Size: 47 3966 2081 Fragments arranged by size: 3966 2081 47 StuI AGG'CCT Cuts at: 1517 2420 2504 2672 2924 3008 3092 1517 Size: 903 84 168 252 84 84 4519 Fragments arranged by size: 4519 903 252 168 84 84 84 StyI C'CwwG_G Cuts at: 513 1065 2004 2239 3533 513 Size: 552 939 235 1294 3074 Fragments arranged by size: 3074 1294 939 552 235 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3228 4121 4138 4291 4476 4921 325 Size: 1255 1648 893 17 153 185 445 1498 Fragments arranged by size: 1648 1498 1255 893 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2094 3242 4340 Size: 274 80 33 51 1443 117 1148 1098 Cuts at: 4340 6052 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1148 1098 274 138 117 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6054 6086 6054 Size: 32 6062 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6094 XbaI T'CTAG_A Cuts at: 3430 3430 Size: 6094 XhoI C'TCGA_G Cuts at: 1943 2931 1943 Size: 988 5106 XmaI C'CCGG_G Cuts at: 1870 3419 1870 Size: 1549 4545 XmnI GAAnn'nnTTC Cuts at: 2504 3092 3176 4223 2504 Size: 588 84 1047 4375 Fragments arranged by size: 4375 1047 588 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3902 276 Size: 53 83 186 936 2368 2468 Fragments arranged by size: 2468 2368 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI