(Circular) (Six-base) MAPSORT of: pFN21AB4035.seq Check: 9829 from: 1 to: 6124 pFN21AB4035 6124 bp With 182 enzymes: SgfI * October 20, 2009 10:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3934 278 Size: 53 83 186 936 2398 2468 Fragments arranged by size: 2468 2398 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2826 3467 1937 Size: 889 641 4594 Fragments arranged by size: 4594 889 641 Acc65I G'GTAC_C Cuts at: 2963 3445 2963 Size: 482 5642 AclI AA'CG_TT Cuts at: 1563 4251 4624 1563 Size: 2688 373 3063 Fragments arranged by size: 3063 2688 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3429 4187 5571 5839 1632 Size: 1797 758 1384 268 1917 Fragments arranged by size: 1917 1797 1384 758 268 AfeI AGC'GCT Cuts at: 3025 3025 Size: 6124 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5927 AflIII A'CryG_T Cuts at: 1203 5023 6000 1203 Size: 3820 977 1327 Fragments arranged by size: 3820 1327 977 AgeI A'CCGG_T Cuts at: 4955 4955 Size: 6124 AhdI GACnn_n'nnGTC Cuts at: 2617 4853 2617 Size: 2236 3888 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6124 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6124 AlwNI CAG_nnn'CTG Cuts at: 2816 3047 3185 3867 5439 2816 Size: 231 138 682 1572 3501 Fragments arranged by size: 3501 1572 682 231 138 ApaLI G'TGCA_C Cuts at: 4181 5337 4181 Size: 1156 4968 ApoI r'AATT_y Cuts at: 1493 2078 2737 3329 3433 3659 3811 5920 1493 Size: 585 659 592 104 226 152 2109 1697 Fragments arranged by size: 2109 1697 659 592 585 226 152 104 AseI AT'TA_AT Cuts at: 160 1032 4678 160 Size: 872 3646 1606 Fragments arranged by size: 3646 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6124 AvaI C'yCGr_G Cuts at: 1870 1943 3449 1870 Size: 73 1506 4545 Fragments arranged by size: 4545 1506 73 BamHI G'GATC_C Cuts at: 3454 3821 3454 Size: 367 5757 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2630 2963 3445 4900 618 Size: 325 217 627 843 333 482 1455 1842 Fragments arranged by size: 1842 1455 843 627 482 333 325 217 BanII G_rGCy'C Cuts at: 1391 3443 1391 Size: 2052 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5153 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4315 4349 1903 Size: 34 2378 34 3678 Fragments arranged by size: 3678 2378 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4016 5226 4016 Size: 1210 4914 BclI T'GATC_A Cuts at: 1548 2139 2385 1548 Size: 591 246 5287 Fragments arranged by size: 5287 591 246 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2286 4735 136 Size: 107 122 71 631 605 614 2449 1525 Fragments arranged by size: 2449 1525 631 614 605 122 107 71 BglII A'GATC_T Cuts at: 2764 3257 6119 2764 Size: 493 2862 2769 Fragments arranged by size: 2862 2769 493 BlpI GC'TnA_GC Cuts at: 2108 3541 2108 Size: 1433 4691 Bme1580I G_kGCm'C Cuts at: 1790 4185 5341 1790 Size: 2395 1156 2573 Fragments arranged by size: 2573 2395 1156 BmgBI CAC'GTC Cuts at: 1318 2619 1318 Size: 1301 4823 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2441 2504 2854 Size: 416 53 83 399 24 1024 63 350 Cuts at: 2854 2879 3855 4813 442 Size: 25 976 958 1753 Fragments arranged by size: 1753 1024 976 958 416 399 350 83 63 53 25 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6124 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4784 1239 Size: 3545 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2189 3295 4249 5114 5412 2189 Size: 1106 954 865 298 2901 Fragments arranged by size: 2901 1106 954 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2604 2689 3195 4787 882 Size: 617 1105 85 506 1592 2219 Fragments arranged by size: 2219 1592 1105 617 506 85 BsaAI yAC'GTr Cuts at: 493 1204 3915 493 Size: 711 2711 2702 Fragments arranged by size: 2711 2702 711 BsaBI GATnn'nnATC Cuts at: 2376 2938 6118 2376 Size: 562 3180 2382 Fragments arranged by size: 3180 2382 562 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3931 4313 4948 275 Size: 53 83 186 936 2398 382 635 1451 Fragments arranged by size: 2398 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3321 4557 4955 5229 5376 5787 3321 Size: 1236 398 274 147 411 3658 Fragments arranged by size: 3658 1236 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2751 2781 1178 Size: 30 165 30 1348 30 4521 Fragments arranged by size: 4521 1348 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2715 3183 1186 Size: 1529 468 4127 Fragments arranged by size: 4127 1529 468 BseYI C'CCAG_C Cuts at: 5327 5823 5327 Size: 496 5628 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2601 2646 2658 3006 1163 Size: 573 865 45 12 348 4281 Fragments arranged by size: 4281 865 573 348 45 12 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3310 3574 4335 5363 1540 Size: 275 186 1309 264 761 1028 2301 Fragments arranged by size: 2301 1309 1028 761 275 264 186 BsiHKAI G_wGCw'C Cuts at: 4185 4270 5341 4185 Size: 85 1071 4968 Fragments arranged by size: 4968 1071 85 BsiWI C'GTAC_G Cuts at: 3294 3294 Size: 6124 BsmI GAATG_Cn' Cuts at: 3635 3728 3635 Size: 93 6031 BsmBI CGTCTCn'nnnn_ Cuts at: 2255 2255 Size: 6124 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3443 4185 4270 5341 1391 Size: 399 1653 742 85 1071 2174 Fragments arranged by size: 2174 1653 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5787 5787 Size: 6124 BspHI T'CATG_A Cuts at: 4013 5743 4013 Size: 1730 4394 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2573 2838 3479 844 Size: 1729 265 641 3489 Fragments arranged by size: 3489 1729 641 265 BsrBI CCG'CTC Cuts at: 1394 1823 4011 1394 Size: 429 2188 3507 Fragments arranged by size: 3507 2188 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4619 4793 65 Size: 1183 3371 174 1396 Fragments arranged by size: 3371 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4768 4955 1700 Size: 110 2958 187 2869 Fragments arranged by size: 2958 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2472 96 Size: 2376 3748 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6124 BssSI C'ACGA_G Cuts at: 3245 4184 5196 3245 Size: 939 1012 4173 Fragments arranged by size: 4173 1012 939 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6124 BstBI TT'CG_AA Cuts at: 4928 4928 Size: 6124 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6124 BstYI r'GATC_y Cuts at: 1972 2764 3257 3454 3821 4206 4223 5675 5761 Size: 792 493 197 367 385 17 1452 86 Cuts at: 5761 5773 6119 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 792 493 385 367 346 197 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4633 Fragments arranged by size: 4633 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5385 Fragments arranged by size: 5385 683 56 BtsI GCAGTG_nn' Cuts at: 3640 4434 4454 3640 Size: 794 20 5310 Fragments arranged by size: 5310 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6124 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6089 DraI TTT'AAA Cuts at: 3428 3428 Size: 6124 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5131 809 Size: 507 3815 1802 Fragments arranged by size: 3815 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3571 4460 6089 Size: 54 1425 50 275 18 1741 889 1629 Cuts at: 6089 8 Size: 43 Fragments arranged by size: 1741 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3571 1537 Size: 275 1759 4090 Fragments arranged by size: 4090 1759 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3033 4054 1353 Size: 1680 1021 3423 Fragments arranged by size: 3423 1680 1021 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4711 5085 5231 387 Size: 1064 138 3122 374 146 1280 Fragments arranged by size: 3122 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3429 4187 4784 5571 5839 1239 Size: 393 1797 758 597 787 268 1524 Fragments arranged by size: 1797 1524 787 758 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2278 2278 Size: 6124 EcoRI G'AATT_C Cuts at: 3329 3433 3811 3329 Size: 104 378 5642 Fragments arranged by size: 5642 378 104 EcoRV GAT'ATC Cuts at: 2936 2936 Size: 6124 FspI TGC'GCA Cuts at: 4630 4630 Size: 6124 HaeII r_GCGC'y Cuts at: 1678 3027 5271 1678 Size: 1349 2244 2531 Fragments arranged by size: 2531 2244 1349 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2603 2635 2981 3013 3124 Size: 32 135 32 1031 32 346 32 111 Cuts at: 3124 3156 4765 4797 4839 4871 1373 Size: 32 1609 32 42 32 2626 Fragments arranged by size: 2626 1609 1031 346 135 111 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2827 3268 3468 3714 669 Size: 988 281 889 441 200 246 3079 Fragments arranged by size: 3079 988 889 441 281 246 200 HindIII A'AGCT_T Cuts at: 748 748 Size: 6124 HpaI GTT'AAC Cuts at: 3714 3714 Size: 6124 KpnI G_GTAC'C Cuts at: 2967 3449 2967 Size: 482 5642 MfeI C'AATT_G Cuts at: 3723 3723 Size: 6124 MluI A'CGCG_T Cuts at: 6000 6000 Size: 6124 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5238 5422 1305 Size: 3933 184 2007 Fragments arranged by size: 3933 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4302 Fragments arranged by size: 4302 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2186 2447 4082 4441 4600 518 Size: 648 1020 261 1635 359 159 2042 Fragments arranged by size: 2042 1635 1020 648 359 261 159 MspA1I CmG'CkG Cuts at: 1606 3282 3540 4217 5365 5610 1606 Size: 1676 258 677 1148 245 2120 Fragments arranged by size: 2120 1676 1148 677 258 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4633 Fragments arranged by size: 4633 939 552 NdeI CA'TA_TG Cuts at: 387 2959 387 Size: 2572 3552 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6124 NotI GC'GGCC_GC Cuts at: 3571 3571 Size: 6124 NspI r_CATG'y Cuts at: 1132 1229 3079 3400 3482 5027 1132 Size: 97 1850 321 82 1545 2229 Fragments arranged by size: 2229 1850 1545 321 97 82 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6124 PciI A'CATG_T Cuts at: 5023 5023 Size: 6124 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6124 PmeI GTTT'AAAC Cuts at: 3428 3428 Size: 6124 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4193 4225 5706 5738 4193 Size: 32 1481 32 4579 Fragments arranged by size: 4579 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6124 PsiI TTA'TAA Cuts at: 3694 3694 Size: 6124 PstI C_TGCA'G Cuts at: 830 2538 3476 830 Size: 1708 938 3478 Fragments arranged by size: 3478 1708 938 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6124 PvuII CAG'CTG Cuts at: 3282 3282 Size: 6124 SalI G'TCGA_C Cuts at: 1936 2825 3466 1936 Size: 889 641 4594 Fragments arranged by size: 4594 889 641 SapI GCTCTTCn'nnn_ Cuts at: 3033 3033 Size: 6124 SbfI CC_TGCA'GG Cuts at: 3476 3476 Size: 6124 ScaI AGT'ACT Cuts at: 2972 4372 6084 2972 Size: 1400 1712 3012 Fragments arranged by size: 3012 1712 1400 SexAI A'CCwGG_T Cuts at: 2671 2689 2671 Size: 18 6106 SfcI C'TryA_G Cuts at: 826 1045 2534 3283 3472 4607 5288 5479 826 Size: 219 1489 749 189 1135 681 191 1471 Fragments arranged by size: 1489 1471 1135 749 681 219 191 189 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6124 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6124 SmaI CCC'GGG Cuts at: 1872 3451 1872 Size: 1579 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2204 3274 4228 5129 5391 820 Size: 197 926 261 1070 954 901 262 1553 Fragments arranged by size: 1553 1070 954 926 901 262 261 197 SnaBI TAC'GTA Cuts at: 493 3915 493 Size: 3422 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6124 SphI G_CATG'C Cuts at: 1132 3400 3482 1132 Size: 2268 82 3774 Fragments arranged by size: 3774 2268 82 SspI AAT'ATT Cuts at: 5 52 4048 5 Size: 47 3996 2081 Fragments arranged by size: 3996 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6124 StyI C'CwwG_G Cuts at: 513 1065 2004 3034 3563 513 Size: 552 939 1030 529 3074 Fragments arranged by size: 3074 1030 939 552 529 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2594 3324 4151 4168 4321 4506 4951 Size: 1255 1014 730 827 17 153 185 445 Cuts at: 4951 325 Size: 1498 Fragments arranged by size: 1498 1255 1014 827 730 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2208 2217 2352 Size: 274 80 33 51 1443 231 9 135 Cuts at: 2352 2472 2970 4370 6082 96 Size: 120 498 1400 1712 138 Fragments arranged by size: 1712 1443 1400 498 274 231 138 135 120 80 51 33 9 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6084 6116 6084 Size: 32 6092 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6124 XbaI T'CTAG_A Cuts at: 3460 3460 Size: 6124 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2185 3341 2185 Size: 1156 4968 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6124 XmaI C'CCGG_G Cuts at: 1870 3449 1870 Size: 1579 4545 XmnI GAAnn'nnTTC Cuts at: 3329 4253 3329 Size: 924 5200 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3932 276 Size: 53 83 186 936 2398 2468 Fragments arranged by size: 2468 2398 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI