Restriction map for
pFN21AB4020
(Circular) (Six-base) MAPSORT of: pFN21AB4020.seq Check: 8663 from: 1 to: 6697
pFN21AB4020 6697 bp
With 182 enzymes: SgfI *
October 20, 2009 10:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3472 3472
Size: 6697
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4507 278
Size: 53 83 186 936 2971 2468
Fragments arranged by size:
2971 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2136 4040 1937
Size: 199 1904 4594
Fragments arranged by size:
4594 1904 199
Acc65I G'GTAC_C
Cuts at: 4018 4018
Size: 6697
AclI AA'CG_TT
Cuts at: 1563 4824 5197 1563
Size: 3261 373 3063
Fragments arranged by size:
3261 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2223 2426 2537 3491 4760 6144 6412 1632
Size: 591 203 111 954 1269 1384 268 1917
Fragments arranged by size:
1917 1384 1269 954 591 268 203 111
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6500
AflIII A'CryG_T
Cuts at: 1203 5596 6573 1203
Size: 4393 977 1327
Fragments arranged by size:
4393 1327 977
AgeI A'CCGG_T
Cuts at: 3447 5528 3447
Size: 2081 4616
AhdI GACnn_n'nnGTC
Cuts at: 5426 5426
Size: 6697
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6697
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3650 1184
Size: 2466 4231
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2604 2636 2604
Size: 32 6665
AlwNI CAG_nnn'CTG
Cuts at: 2746 3070 3509 3647 4440 6012 2746
Size: 324 439 138 793 1572 3431
Fragments arranged by size:
3431 1572 793 439 324 138
ApaI G_GGCC'C
Cuts at: 2755 2755
Size: 6697
ApaLI G'TGCA_C
Cuts at: 4754 5910 4754
Size: 1156 5541
ApoI r'AATT_y
Cuts at: 1493 4006 4232 4384 6493 1493
Size: 2513 226 152 2109 1697
Fragments arranged by size:
2513 2109 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2026 2026
Size: 6697
AseI AT'TA_AT
Cuts at: 160 1032 5251 160
Size: 872 4219 1606
Fragments arranged by size:
4219 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6697
AvaI C'yCGr_G
Cuts at: 1870 1943 2258 3721 4022 1870
Size: 73 315 1463 301 4545
Fragments arranged by size:
4545 1463 315 301 73
AvrII C'CTAG_G
Cuts at: 2530 2530
Size: 6697
BamHI G'GATC_C
Cuts at: 4027 4394 4027
Size: 367 6330
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4018 5473 618
Size: 325 217 627 2231 1455 1842
Fragments arranged by size:
2231 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2118 2259 2755 3820 3977 4016 1391
Size: 727 141 496 1065 157 39 4072
Fragments arranged by size:
4072 1065 727 496 157 141 39
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2804 928
Size: 971 905 4821
Fragments arranged by size:
4821 971 905
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2881 2915 4888 4922 1903
Size: 34 944 34 1973 34 3678
Fragments arranged by size:
3678 1973 944 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4589 5799 4589
Size: 1210 5487
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6697
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2249 2954 3794
Size: 107 122 71 631 605 577 705 840
Cuts at: 3794 5308 136
Size: 1514 1525
Fragments arranged by size:
1525 1514 840 705 631 605 577 122
107 71
BglII A'GATC_T
Cuts at: 6692 6692
Size: 6697
BlpI GC'TnA_GC
Cuts at: 2321 2921 4114 2321
Size: 600 1193 4904
Fragments arranged by size:
4904 1193 600
Bme1580I G_kGCm'C
Cuts at: 1790 2755 4758 5914 1790
Size: 965 2003 1156 2573
Fragments arranged by size:
2573 2003 1156 965
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6697
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3615 4428 5386
Size: 416 53 83 399 24 2198 813 958
Cuts at: 5386 442
Size: 1753
Fragments arranged by size:
2198 1753 958 813 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6697
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2472 2472
Size: 6697
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2336 2435 2498 2813 2915 2993 3287 3841
Size: 1097 99 63 315 102 78 294 554
Cuts at: 3841 3940 5357 1239
Size: 99 1417 2579
Fragments arranged by size:
2579 1417 1097 554 315 294 102 99
99 78 63
Bpu10I CC'TnA_GC
Cuts at: 2394 3811 2394
Size: 1417 5280
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2540 4822 5687 5985 2540
Size: 2282 865 298 3252
Fragments arranged by size:
3252 2282 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2003 2648 2666 2961 5360 882
Size: 617 504 645 18 295 2399 2219
Fragments arranged by size:
2399 2219 645 617 504 295 18
BsaAI yAC'GTr
Cuts at: 493 1204 4488 493
Size: 711 3284 2702
Fragments arranged by size:
3284 2702 711
BsaBI GATnn'nnATC
Cuts at: 6691 6691
Size: 6697
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4504 4886 5521 275
Size: 53 83 186 936 2971 382 635 1451
Fragments arranged by size:
2971 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3447 5130 5528 5802 5949 6360 3447
Size: 1683 398 274 147 411 3784
Fragments arranged by size:
3784 1683 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2000 2030 3338 3368 3689
Size: 30 165 30 597 30 1308 30 321
Cuts at: 3689 3719 3807 3837 1178
Size: 30 88 30 4038
Fragments arranged by size:
4038 1308 597 321 165 88 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2351 2657 2888 3167 3170 3347 3359 3829
Size: 1165 306 231 279 3 177 12 470
Cuts at: 3829 1186
Size: 4054
Fragments arranged by size:
4054 1165 470 306 279 231 177 12
3
BseYI C'CCAG_C
Cuts at: 2019 2754 2916 3176 3476 3509 5900 6396 2019
Size: 735 162 260 300 33 2391 496 2320
Fragments arranged by size:
2391 2320 735 496 300 260 162 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6124
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2146 4147 4908 5936 1540
Size: 275 186 145 2001 761 1028 2301
Fragments arranged by size:
2301 2001 1028 761 275 186 145
BsiHKAI G_wGCw'C
Cuts at: 2118 2259 2453 4758 4843 5914 2118
Size: 141 194 2305 85 1071 2901
Fragments arranged by size:
2901 2305 1071 194 141 85
BsiWI C'GTAC_G
Cuts at: 3584 3584
Size: 6697
BsmI GAATG_Cn'
Cuts at: 4208 4301 4208
Size: 93 6604
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2118 2259 2453 2755 3820 3977 4016
Size: 399 328 141 194 302 1065 157 39
Cuts at: 4016 4758 4843 5914 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 1065 742 399 328 302 194
157 141 85 39
BspEI T'CCGG_A
Cuts at: 6360 6360
Size: 6697
BspHI T'CATG_A
Cuts at: 2668 4586 6316 2668
Size: 1918 1730 3049
Fragments arranged by size:
3049 1918 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2115 3472 3693 3753 3782 4052 844
Size: 1271 1357 221 60 29 270 3489
Fragments arranged by size:
3489 1357 1271 270 221 60 29
BsrBI CCG'CTC
Cuts at: 1394 1823 2995 3031 3936 4584 1394
Size: 429 1172 36 905 648 3507
Fragments arranged by size:
3507 1172 905 648 429 36
BsrDI GCAATG_nn'
Cuts at: 65 1248 5192 5366 65
Size: 1183 3944 174 1396
Fragments arranged by size:
3944 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2721 3006 3447 5341 5528 1700
Size: 110 911 285 441 1894 187 2869
Fragments arranged by size:
2869 1894 911 441 285 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6697
BssHII G'CGCG_C
Cuts at: 1925 2026 2299 2357 1925
Size: 101 273 58 6265
Fragments arranged by size:
6265 273 101 58
BssSI C'ACGA_G
Cuts at: 2870 4757 5769 2870
Size: 1887 1012 3798
Fragments arranged by size:
3798 1887 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2497 2746 794
Size: 1703 249 4745
Fragments arranged by size:
4745 1703 249
BstBI TT'CG_AA
Cuts at: 5501 5501
Size: 6697
BstEII G'GTnAC_C
Cuts at: 1184 2378 3928 1184
Size: 1194 1550 3953
Fragments arranged by size:
3953 1550 1194
BstYI r'GATC_y
Cuts at: 1972 3120 4027 4394 4779 4796 6248 6334 6346
Size: 1148 907 367 385 17 1452 86 12
Cuts at: 6346 6692 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1148 907 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3199 3327 3393 3700 513
Size: 552 939 1195 128 66 307 3510
Fragments arranged by size:
3510 1195 939 552 307 128 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5958
Fragments arranged by size:
5958 683 56
BtsI GCAGTG_nn'
Cuts at: 2451 3709 3892 3904 4213 5007 5027 2451
Size: 1258 183 12 309 794 20 4121
Fragments arranged by size:
4121 1258 794 309 183 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6697
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6662
DraI TTT'AAA
Cuts at: 4001 4001
Size: 6697
DraIII CAC_nnn'GTG
Cuts at: 3236 3236
Size: 6697
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3926 5704 809
Size: 507 2610 1778 1802
Fragments arranged by size:
2610 1802 1778 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2511 3039 3202
Size: 54 1425 50 275 18 681 528 163
Cuts at: 3202 3324 3618 4144 5033 6662 8
Size: 122 294 526 889 1629 43
Fragments arranged by size:
1629 1425 889 681 528 526 294 275
163 122 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4144 1537
Size: 275 2332 4090
Fragments arranged by size:
4090 2332 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2199 3853 4627 1353
Size: 846 1654 774 3423
Fragments arranged by size:
3423 1654 846 774
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2744 3035 3843 5284 5658 5804
Size: 1064 138 1155 291 808 1441 374 146
Cuts at: 5804 387
Size: 1280
Fragments arranged by size:
1441 1280 1155 1064 808 374 291 146
138
EcoICRI GAG'CTC
Cuts at: 2116 2257 2116
Size: 141 6556
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2223 2336 2426 2435 2498 2537 2813
Size: 393 591 113 90 9 63 39 276
Cuts at: 2813 2915 2993 3287 3491 3841 3940 4760 5357
Size: 102 78 294 204 350 99 820 597
Cuts at: 5357 6144 6412 1239
Size: 787 268 1524
Fragments arranged by size:
1524 820 787 597 591 393 350 294
276 268 204 113 102 99 90 78
63 39 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2534 2534
Size: 6697
EcoO109I rG'GnC_Cy
Cuts at: 2104 2410 3727 3771 2104
Size: 306 1317 44 5030
Fragments arranged by size:
5030 1317 306 44
EcoRI G'AATT_C
Cuts at: 4006 4384 4006
Size: 378 6319
FspI TGC'GCA
Cuts at: 3368 3626 5203 3368
Size: 258 1577 4862
Fragments arranged by size:
4862 1577 258
HaeII r_GCGC'y
Cuts at: 1678 2665 3418 5844 1678
Size: 987 753 2426 2531
Fragments arranged by size:
2531 2426 987 753
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2000 2032 2377 2409 2894
Size: 32 135 32 428 32 345 32 485
Cuts at: 2894 2926 3374 3406 3782 3814 5338 5370 5412
Size: 32 448 32 376 32 1524 32 42
Cuts at: 5412 5444 1373
Size: 32 2626
Fragments arranged by size:
2626 1524 485 448 428 376 345 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4041 4287 669
Size: 988 281 2103 246 3079
Fragments arranged by size:
3079 2103 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2516 2960 748
Size: 1768 444 4485
Fragments arranged by size:
4485 1768 444
HpaI GTT'AAC
Cuts at: 4287 4287
Size: 6697
KpnI G_GTAC'C
Cuts at: 4022 4022
Size: 6697
MfeI C'AATT_G
Cuts at: 3400 4296 3400
Size: 896 5801
MluI A'CGCG_T
Cuts at: 6573 6573
Size: 6697
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5811 5995 1305
Size: 4506 184 2007
Fragments arranged by size:
4506 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2513 3620 10
Size: 54 1425 343 681 1107 3087
Fragments arranged by size:
3087 1425 1107 681 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4655 5014 5173 518
Size: 648 3489 359 159 2042
Fragments arranged by size:
3489 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2023 2040 2065 2314 2941 3070 3734 3807
Size: 417 17 25 249 627 129 664 73
Cuts at: 3807 4113 4790 5938 6183 1606
Size: 306 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 664 627 417 306 249
245 129 73 25 17
NaeI GCC'GGC
Cuts at: 2723 3008 2723
Size: 285 6412
NcoI C'CATG_G
Cuts at: 513 1065 2004 3393 513
Size: 552 939 1389 3817
Fragments arranged by size:
3817 1389 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6697
NgoMIV G'CCGG_C
Cuts at: 2721 3006 2721
Size: 285 6412
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6697
NotI GC'GGCC_GC
Cuts at: 4144 4144
Size: 6697
NspI r_CATG'y
Cuts at: 1132 1229 4055 5600 1132
Size: 97 2826 1545 2229
Fragments arranged by size:
2826 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6697
PciI A'CATG_T
Cuts at: 5596 5596
Size: 6697
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3394 1108
Size: 2286 4411
PmeI GTTT'AAAC
Cuts at: 4001 4001
Size: 6697
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4766 4798 6279 6311 4766
Size: 32 1481 32 5152
Fragments arranged by size:
5152 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2104 2410 3727 3771 2104
Size: 306 1317 44 5030
Fragments arranged by size:
5030 1317 306 44
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6697
PsiI TTA'TAA
Cuts at: 4267 4267
Size: 6697
PspOMI G'GGCC_C
Cuts at: 2751 2751
Size: 6697
PstI C_TGCA'G
Cuts at: 830 2094 2112 2313 2598 2742 2883 3505 3716
Size: 1264 18 201 285 144 141 622 211
Cuts at: 3716 3989 4049 830
Size: 273 60 3478
Fragments arranged by size:
3478 1264 622 285 273 211 201 144
141 60 18
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6697
PvuII CAG'CTG
Cuts at: 2314 2941 3070 2314
Size: 627 129 5941
Fragments arranged by size:
5941 627 129
SacI G_AGCT'C
Cuts at: 2118 2259 2118
Size: 141 6556
SalI G'TCGA_C
Cuts at: 1936 4039 1936
Size: 2103 4594
SanDI GG'GwC_CC
Cuts at: 3727 3727
Size: 6697
SapI GCTCTTCn'nnn_
Cuts at: 3853 3853
Size: 6697
SbfI CC_TGCA'GG
Cuts at: 2112 4049 2112
Size: 1937 4760
ScaI AGT'ACT
Cuts at: 4945 6657 4945
Size: 1712 4985
SexAI A'CCwGG_T
Cuts at: 2766 2853 3537 2766
Size: 87 684 5926
Fragments arranged by size:
5926 684 87
SfcI C'TryA_G
Cuts at: 826 1045 2090 2108 2309 2594 2635 2738 2879
Size: 219 1045 18 201 285 41 103 141
Cuts at: 2879 3501 3712 3985 4045 5180 5861 6052 826
Size: 622 211 273 60 1135 681 191 1471
Fragments arranged by size:
1471 1135 1045 681 622 285 273 219
211 201 191 141 103 60 41 18
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6697
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6697
SmaI CCC'GGG
Cuts at: 1872 4024 1872
Size: 2152 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2519 4801 5702 5964 820
Size: 197 926 576 2282 901 262 1553
Fragments arranged by size:
2282 1553 926 901 576 262 197
SnaBI TAC'GTA
Cuts at: 493 4488 493
Size: 3995 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6697
SphI G_CATG'C
Cuts at: 1132 4055 1132
Size: 2923 3774
SspI AAT'ATT
Cuts at: 5 52 4621 5
Size: 47 4569 2081
Fragments arranged by size:
4569 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6697
StyI C'CwwG_G
Cuts at: 513 1065 2004 2292 2433 2530 2949 3393 4136
Size: 552 939 288 141 97 419 444 743
Cuts at: 4136 513
Size: 3074
Fragments arranged by size:
3074 939 743 552 444 419 288 141
97
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3533 4724 4741 4894 5079 5524 325
Size: 1255 1953 1191 17 153 185 445 1498
Fragments arranged by size:
1953 1498 1255 1191 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3738 4943 6655
Size: 274 80 33 51 1443 1761 1205 1712
Cuts at: 6655 96
Size: 138
Fragments arranged by size:
1761 1712 1443 1205 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2692 2724 3689 3721 3807 3839 6657 6689 2692
Size: 32 965 32 86 32 2818 32 2700
Fragments arranged by size:
2818 2700 965 86 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2014 1316
Size: 698 5999
XbaI T'CTAG_A
Cuts at: 4033 4033
Size: 6697
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3233 3628 3233
Size: 395 6302
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6697
XmaI C'CCGG_G
Cuts at: 1870 4022 1870
Size: 2152 4545
XmnI GAAnn'nnTTC
Cuts at: 4826 4826
Size: 6697
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4505 276
Size: 53 83 186 936 2971 2468
Fragments arranged by size:
2971 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI BaeI BbeI BbvCI BfrBI BsmBI BstXI BstZ17I
Bsu36I EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII
SfiI SfoI SrfI SwaI