Restriction map for
pFN21AB3734
(Circular) (Six-base) MAPSORT of: pfn21a_k1.seq Check: 6820 from: 1 to: 5064
pFN21A_K1
With 182 enzymes: SgfI *
June 23, 2008 09:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2164 2874 278
Size: 53 83 186 936 628 710 2468
Fragments arranged by size:
2468 936 710 628 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2407 1937
Size: 470 4594
Acc65I G'GTAC_C
Cuts at: 2385 2385
Size: 5064
AclI AA'CG_TT
Cuts at: 1563 3191 3564 1563
Size: 1628 373 3063
Fragments arranged by size:
3063 1628 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2055 2258 3127 4511 4779 1632
Size: 423 203 869 1384 268 1917
Fragments arranged by size:
1917 1384 869 423 268 203
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4867
AflIII A'CryG_T
Cuts at: 1203 2044 3963 4940 1203
Size: 841 1919 977 1327
Fragments arranged by size:
1919 1327 977 841
AgeI A'CCGG_T
Cuts at: 3895 3895
Size: 5064
AhdI GACnn_n'nnGTC
Cuts at: 3793 3793
Size: 5064
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5064
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5064
AlwNI CAG_nnn'CTG
Cuts at: 2807 4379 2807
Size: 1572 3492
ApaLI G'TGCA_C
Cuts at: 3121 4277 3121
Size: 1156 3908
ApoI r'AATT_y
Cuts at: 1493 2279 2373 2599 2751 4860 1493
Size: 786 94 226 152 2109 1697
Fragments arranged by size:
2109 1697 786 226 152 94
AseI AT'TA_AT
Cuts at: 160 1032 3618 160
Size: 872 2586 1606
Fragments arranged by size:
2586 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5064
AvaI C'yCGr_G
Cuts at: 1870 1943 2219 2389 1870
Size: 73 276 170 4545
Fragments arranged by size:
4545 276 170 73
BamHI G'GATC_C
Cuts at: 2394 2761 2394
Size: 367 4697
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2385 3840 618
Size: 325 217 627 598 1455 1842
Fragments arranged by size:
1842 1455 627 598 325 217
BanII G_rGCy'C
Cuts at: 1391 2383 1391
Size: 992 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4093
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3255 3289 1903
Size: 34 1318 34 3678
Fragments arranged by size:
3678 1318 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2956 4166 2956
Size: 1210 3854
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5064
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3675 136
Size: 107 122 71 631 605 2003 1525
Fragments arranged by size:
2003 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5059 5059
Size: 5064
BlpI GC'TnA_GC
Cuts at: 2481 2481
Size: 5064
Bme1580I G_kGCm'C
Cuts at: 1790 3125 4281 1790
Size: 1335 1156 2573
Fragments arranged by size:
2573 1335 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5064
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2795 3753 442
Size: 416 53 83 399 24 1378 958 1753
Fragments arranged by size:
1753 1378 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5064
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3724 1239
Size: 2485 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2285 3189 4054 4352 2285
Size: 904 865 298 2997
Fragments arranged by size:
2997 904 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3727 882
Size: 617 2228 2219
Fragments arranged by size:
2228 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2855 493
Size: 711 1651 2702
Fragments arranged by size:
2702 1651 711
BsaBI GATnn'nnATC
Cuts at: 5058 5058
Size: 5064
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2161 2871 3253 3888
Size: 53 83 186 936 628 710 382 635
Cuts at: 3888 275
Size: 1451
Fragments arranged by size:
1451 936 710 635 628 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2287 3497 3895 4169 4316 4727 2287
Size: 1210 398 274 147 411 2624
Fragments arranged by size:
2624 1210 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4839
Fragments arranged by size:
4839 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5064
BseYI C'CCAG_C
Cuts at: 2131 4267 4763 2131
Size: 2136 496 2432
Fragments arranged by size:
2432 2136 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4491
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2514 3275 4303 1540
Size: 275 186 513 761 1028 2301
Fragments arranged by size:
2301 1028 761 513 275 186
BsiHKAI G_wGCw'C
Cuts at: 3125 3210 4281 3125
Size: 85 1071 3908
Fragments arranged by size:
3908 1071 85
BsmI GAATG_Cn'
Cuts at: 2575 2668 2575
Size: 93 4971
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2383 3125 3210 4281 1391
Size: 399 593 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 593 399 85
BspEI T'CCGG_A
Cuts at: 4727 4727
Size: 5064
BspHI T'CATG_A
Cuts at: 2953 4683 2953
Size: 1730 3334
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2419 844
Size: 1575 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2951 1394
Size: 429 1128 3507
Fragments arranged by size:
3507 1128 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3559 3733 65
Size: 1183 2311 174 1396
Fragments arranged by size:
2311 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3708 3895 1700
Size: 110 1898 187 2869
Fragments arranged by size:
2869 1898 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5064
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5064
BssSI C'ACGA_G
Cuts at: 3124 4136 3124
Size: 1012 4052
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5064
BstBI TT'CG_AA
Cuts at: 3868 3868
Size: 5064
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5064
BstYI r'GATC_y
Cuts at: 1972 2394 2761 3146 3163 4615 4701 4713 5059
Size: 422 367 385 17 1452 86 12 346
Cuts at: 5059 1972
Size: 1977
Fragments arranged by size:
1977 1452 422 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3573
Fragments arranged by size:
3573 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4325
Fragments arranged by size:
4325 683 56
BtsI GCAGTG_nn'
Cuts at: 2580 3374 3394 2580
Size: 794 20 4250
Fragments arranged by size:
4250 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5064
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5029
DraI TTT'AAA
Cuts at: 2368 2368
Size: 5064
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4071 809
Size: 507 2755 1802
Fragments arranged by size:
2755 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2511 3400 5029
Size: 54 1425 50 275 18 681 889 1629
Cuts at: 5029 8
Size: 43
Fragments arranged by size:
1629 1425 889 681 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2511 1537
Size: 275 699 4090
Fragments arranged by size:
4090 699 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2994 1353
Size: 1641 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2200 3651 4025 4171 387
Size: 1064 138 611 1451 374 146 1280
Fragments arranged by size:
1451 1280 1064 611 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2055 2258 3127 3724 4511 4779 1239
Size: 393 423 203 869 597 787 268 1524
Fragments arranged by size:
1524 869 787 597 423 393 268 203
EcoRI G'AATT_C
Cuts at: 2373 2751 2373
Size: 378 4686
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2169 2169
Size: 5064
FspI TGC'GCA
Cuts at: 3570 3570
Size: 5064
HaeII r_GCGC'y
Cuts at: 1678 4211 1678
Size: 2533 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2181 2213 3705 3737 3779
Size: 32 135 32 609 32 1492 32 42
Cuts at: 3779 3811 1373
Size: 32 2626
Fragments arranged by size:
2626 1492 609 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2408 2654 669
Size: 988 281 470 246 3079
Fragments arranged by size:
3079 988 470 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5064
HpaI GTT'AAC
Cuts at: 2654 2654
Size: 5064
KpnI G_GTAC'C
Cuts at: 2389 2389
Size: 5064
MfeI C'AATT_G
Cuts at: 2663 2663
Size: 5064
MluI A'CGCG_T
Cuts at: 4940 4940
Size: 5064
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4178 4362 1305
Size: 2873 184 2007
Fragments arranged by size:
2873 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3242
Fragments arranged by size:
3242 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3022 3381 3540 518
Size: 648 1856 359 159 2042
Fragments arranged by size:
2042 1856 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2480 3157 4305 4550 1606
Size: 874 677 1148 245 2120
Fragments arranged by size:
2120 1148 874 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3573
Fragments arranged by size:
3573 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5064
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5064
NotI GC'GGCC_GC
Cuts at: 2511 2511
Size: 5064
NspI r_CATG'y
Cuts at: 1132 1229 2422 3967 1132
Size: 97 1193 1545 2229
Fragments arranged by size:
2229 1545 1193 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5064
PciI A'CATG_T
Cuts at: 3963 3963
Size: 5064
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5064
PmeI GTTT'AAAC
Cuts at: 2368 2368
Size: 5064
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3133 3165 4646 4678 3133
Size: 32 1481 32 3519
Fragments arranged by size:
3519 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5064
PsiI TTA'TAA
Cuts at: 2634 2634
Size: 5064
PstI C_TGCA'G
Cuts at: 830 2416 830
Size: 1586 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5064
SalI G'TCGA_C
Cuts at: 1936 2406 1936
Size: 470 4594
SbfI CC_TGCA'GG
Cuts at: 2416 2416
Size: 5064
ScaI AGT'ACT
Cuts at: 3312 5024 3312
Size: 1712 3352
SfcI C'TryA_G
Cuts at: 826 1045 2412 3547 4228 4419 826
Size: 219 1367 1135 681 191 1471
Fragments arranged by size:
1471 1367 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5064
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5064
SmaI CCC'GGG
Cuts at: 1872 2221 2391 1872
Size: 349 170 4545
Fragments arranged by size:
4545 349 170
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2300 3168 4069 4331 820
Size: 197 926 357 868 901 262 1553
Fragments arranged by size:
1553 926 901 868 357 262 197
SnaBI TAC'GTA
Cuts at: 493 2855 493
Size: 2362 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5064
SphI G_CATG'C
Cuts at: 1132 2422 1132
Size: 1290 3774
SspI AAT'ATT
Cuts at: 5 52 2988 5
Size: 47 2936 2081
Fragments arranged by size:
2936 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5064
StyI C'CwwG_G
Cuts at: 513 1065 2004 2503 513
Size: 552 939 499 3074
Fragments arranged by size:
3074 939 552 499
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2123 3091 3108 3261 3446 3891 325
Size: 1255 543 968 17 153 185 445 1498
Fragments arranged by size:
1498 1255 968 543 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3310 5022 96
Size: 274 80 33 51 1443 1333 1712 138
Fragments arranged by size:
1712 1443 1333 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5024 5056 5024
Size: 32 5032
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5064
XbaI T'CTAG_A
Cuts at: 2400 2400
Size: 5064
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5064
XmaI C'CCGG_G
Cuts at: 1870 2219 2389 1870
Size: 349 170 4545
Fragments arranged by size:
4545 349 170
XmnI GAAnn'nnTTC
Cuts at: 3193 3193
Size: 5064
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2162 2872 276
Size: 53 83 186 936 628 710 2468
Fragments arranged by size:
2468 936 710 628 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI