(Circular) (Six-base) MAPSORT of: pFN21AB3732.seq Check: 6883 from: 1 to: 6088 pFN21AB3732 6088 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3898 278 Size: 53 83 186 936 2362 2468 Fragments arranged by size: 2468 2362 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3431 1937 Size: 1494 4594 Acc65I G'GTAC_C Cuts at: 3409 3409 Size: 6088 AclI AA'CG_TT Cuts at: 1563 4215 4588 1563 Size: 2652 373 3063 Fragments arranged by size: 3063 2652 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2124 2794 3107 3129 4151 5535 5803 1632 Size: 492 670 313 22 1022 1384 268 1917 Fragments arranged by size: 1917 1384 1022 670 492 313 268 22 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5891 AflIII A'CryG_T Cuts at: 1203 2372 4987 5964 1203 Size: 1169 2615 977 1327 Fragments arranged by size: 2615 1327 1169 977 AgeI A'CCGG_T Cuts at: 4919 4919 Size: 6088 AhdI GACnn_n'nnGTC Cuts at: 4817 4817 Size: 6088 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6088 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6088 AlwNI CAG_nnn'CTG Cuts at: 2718 3831 5403 2718 Size: 1113 1572 3403 Fragments arranged by size: 3403 1572 1113 ApaLI G'TGCA_C Cuts at: 4145 5301 4145 Size: 1156 4932 ApoI r'AATT_y Cuts at: 1493 2735 3397 3623 3775 5884 1493 Size: 1242 662 226 152 2109 1697 Fragments arranged by size: 2109 1697 1242 662 226 152 AseI AT'TA_AT Cuts at: 160 1032 4642 160 Size: 872 3610 1606 Fragments arranged by size: 3610 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6088 AvaI C'yCGr_G Cuts at: 1870 1943 3154 3413 1870 Size: 73 1211 259 4545 Fragments arranged by size: 4545 1211 259 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2350 2383 2350 Size: 33 6055 BamHI G'GATC_C Cuts at: 3418 3785 3418 Size: 367 5721 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3409 4864 618 Size: 325 217 627 1622 1455 1842 Fragments arranged by size: 1842 1622 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2322 2646 2667 3407 1391 Size: 931 324 21 740 4072 Fragments arranged by size: 4072 931 740 324 21 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2754 2907 3078 3135 928 Size: 971 855 153 171 57 3881 Fragments arranged by size: 3881 971 855 171 153 57 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2466 2500 4279 4313 1903 Size: 34 529 34 1779 34 3678 Fragments arranged by size: 3678 1779 529 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3980 5190 3980 Size: 1210 4878 BclI T'GATC_A Cuts at: 1548 1548 Size: 6088 BfrBI ATG'CAT Cuts at: 2511 2511 Size: 6088 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2693 4699 136 Size: 107 122 71 631 605 1021 2006 1525 Fragments arranged by size: 2006 1525 1021 631 605 122 107 71 BglII A'GATC_T Cuts at: 3283 6083 3283 Size: 2800 3288 BlpI GC'TnA_GC Cuts at: 3505 3505 Size: 6088 Bme1580I G_kGCm'C Cuts at: 1790 2254 2634 4149 5305 1790 Size: 464 380 1515 1156 2573 Fragments arranged by size: 2573 1515 1156 464 380 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6088 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2292 3819 4777 Size: 416 53 83 399 24 875 1527 958 Cuts at: 4777 442 Size: 1753 Fragments arranged by size: 1753 1527 958 875 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6088 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4748 1239 Size: 3509 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2230 4213 5078 5376 2230 Size: 1983 865 298 2942 Fragments arranged by size: 2942 1983 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4751 882 Size: 617 3252 2219 Fragments arranged by size: 3252 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2373 3244 3879 493 Size: 711 1169 871 635 2702 Fragments arranged by size: 2702 1169 871 711 635 BsaBI GATnn'nnATC Cuts at: 6082 6082 Size: 6088 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3057 3895 4277 4912 Size: 53 83 186 936 1524 838 382 635 Cuts at: 4912 275 Size: 1451 Fragments arranged by size: 1524 1451 936 838 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4521 4919 5193 5340 5751 4521 Size: 398 274 147 411 4858 Fragments arranged by size: 4858 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2927 2957 1178 Size: 30 165 30 1524 30 4309 Fragments arranged by size: 4309 1524 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2285 2597 2906 1186 Size: 1099 312 309 4368 Fragments arranged by size: 4368 1099 312 309 BseYI C'CCAG_C Cuts at: 5291 5787 5291 Size: 496 5592 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3308 1163 Size: 573 1572 3943 Fragments arranged by size: 3943 1572 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3538 4299 5327 1540 Size: 275 186 1537 761 1028 2301 Fragments arranged by size: 2301 1537 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2322 2667 4149 4234 5305 2322 Size: 345 1482 85 1071 3105 Fragments arranged by size: 3105 1482 1071 345 85 BsmI GAATG_Cn' Cuts at: 3344 3599 3692 3344 Size: 255 93 5740 Fragments arranged by size: 5740 255 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2254 2322 2634 2646 2667 3407 4149 Size: 399 464 68 312 12 21 740 742 Cuts at: 4149 4234 5305 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 740 464 399 312 85 68 21 12 BspEI T'CCGG_A Cuts at: 5751 5751 Size: 6088 BspHI T'CATG_A Cuts at: 3977 5707 3977 Size: 1730 4358 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3443 844 Size: 2599 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3975 1394 Size: 429 2152 3507 Fragments arranged by size: 3507 2152 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4583 4757 65 Size: 1183 3335 174 1396 Fragments arranged by size: 3335 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4732 4919 1700 Size: 110 2922 187 2869 Fragments arranged by size: 2922 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6088 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6088 BssSI C'ACGA_G Cuts at: 4148 5160 4148 Size: 1012 5076 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6088 BstBI TT'CG_AA Cuts at: 4892 4892 Size: 6088 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6088 BstXI CCAn_nnnn'nTGG Cuts at: 2854 2854 Size: 6088 BstYI r'GATC_y Cuts at: 1972 3283 3418 3785 4170 4187 5639 5725 5737 Size: 1311 135 367 385 17 1452 86 12 Cuts at: 5737 6083 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1311 385 367 346 135 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5536 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5349 Fragments arranged by size: 5349 683 56 BtsI GCAGTG_nn' Cuts at: 3015 3604 4398 4418 3015 Size: 589 794 20 4685 Fragments arranged by size: 4685 794 589 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6088 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6053 DraI TTT'AAA Cuts at: 3392 3392 Size: 6088 DraIII CAC_nnn'GTG Cuts at: 3244 3244 Size: 6088 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2240 5095 809 Size: 507 924 2855 1802 Fragments arranged by size: 2855 1802 924 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2562 3257 3535 Size: 54 1425 50 275 18 732 695 278 Cuts at: 3535 4424 6053 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 732 695 278 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3535 1537 Size: 275 1723 4090 Fragments arranged by size: 4090 1723 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2117 2604 3359 4018 1353 Size: 764 487 755 659 3423 Fragments arranged by size: 3423 764 755 659 487 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4675 5049 5195 387 Size: 1064 138 3086 374 146 1280 Fragments arranged by size: 3086 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2320 2665 2320 Size: 345 5743 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2124 2794 3107 3129 4151 4748 5535 Size: 393 492 670 313 22 1022 597 787 Cuts at: 5535 5803 1239 Size: 268 1524 Fragments arranged by size: 1524 1022 787 670 597 492 393 313 268 22 EcoNI CCTnn'n_nnAGG Cuts at: 3122 3272 3122 Size: 150 5938 EcoRI G'AATT_C Cuts at: 3397 3775 3397 Size: 378 5710 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2410 2815 3000 2410 Size: 405 185 5498 Fragments arranged by size: 5498 405 185 FspI TGC'GCA Cuts at: 4594 4594 Size: 6088 HaeII r_GCGC'y Cuts at: 1678 5235 1678 Size: 3557 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2927 2959 4729 4761 4803 Size: 32 135 32 1355 32 1770 32 42 Cuts at: 4803 4835 1373 Size: 32 2626 Fragments arranged by size: 2626 1770 1355 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2797 3432 3678 669 Size: 988 281 859 635 246 3079 Fragments arranged by size: 3079 988 859 635 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6088 HpaI GTT'AAC Cuts at: 3678 3678 Size: 6088 KpnI G_GTAC'C Cuts at: 3413 3413 Size: 6088 MfeI C'AATT_G Cuts at: 3687 3687 Size: 6088 MluI A'CGCG_T Cuts at: 5964 5964 Size: 6088 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5202 5386 1305 Size: 3897 184 2007 Fragments arranged by size: 3897 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2564 3259 10 Size: 54 1425 343 732 695 2839 Fragments arranged by size: 2839 1425 732 695 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2223 3332 4046 4405 4564 518 Size: 648 1057 1109 714 359 159 2042 Fragments arranged by size: 2042 1109 1057 714 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2364 3025 3504 4181 5329 5574 1606 Size: 758 661 479 677 1148 245 2120 Fragments arranged by size: 2120 1148 758 677 661 479 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5536 NdeI CA'TA_TG Cuts at: 387 387 Size: 6088 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6088 NotI GC'GGCC_GC Cuts at: 3535 3535 Size: 6088 NsiI A_TGCA'T Cuts at: 2513 2513 Size: 6088 NspI r_CATG'y Cuts at: 1132 1229 3079 3446 4991 1132 Size: 97 1850 367 1545 2229 Fragments arranged by size: 2229 1850 1545 367 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6088 PciI A'CATG_T Cuts at: 4987 4987 Size: 6088 PflMI CCAn_nnn'nTGG Cuts at: 1108 2786 1108 Size: 1678 4410 PmeI GTTT'AAAC Cuts at: 3392 3392 Size: 6088 PmlI CAC'GTG Cuts at: 2373 3244 2373 Size: 871 5217 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4157 4189 5670 5702 4157 Size: 32 1481 32 4543 Fragments arranged by size: 4543 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6088 PsiI TTA'TAA Cuts at: 3658 3658 Size: 6088 PstI C_TGCA'G Cuts at: 830 2619 3440 830 Size: 1789 821 3478 Fragments arranged by size: 3478 1789 821 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6088 PvuII CAG'CTG Cuts at: 3025 3025 Size: 6088 SacI G_AGCT'C Cuts at: 2322 2667 2322 Size: 345 5743 SalI G'TCGA_C Cuts at: 1936 3430 1936 Size: 1494 4594 SapI GCTCTTCn'nnn_ Cuts at: 2117 2604 2117 Size: 487 5601 SbfI CC_TGCA'GG Cuts at: 3440 3440 Size: 6088 ScaI AGT'ACT Cuts at: 4336 6048 4336 Size: 1712 4376 SfcI C'TryA_G Cuts at: 826 1045 2615 3436 4571 5252 5443 826 Size: 219 1570 821 1135 681 191 1471 Fragments arranged by size: 1570 1471 1135 821 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6088 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6088 SmaI CCC'GGG Cuts at: 1872 3156 3415 1872 Size: 1284 259 4545 Fragments arranged by size: 4545 1284 259 SmlI C'TyrA_G Cuts at: 820 1017 1943 2245 4192 5093 5355 820 Size: 197 926 302 1947 901 262 1553 Fragments arranged by size: 1947 1553 926 901 302 262 197 SnaBI TAC'GTA Cuts at: 493 3879 493 Size: 3386 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6088 SphI G_CATG'C Cuts at: 1132 3446 1132 Size: 2314 3774 SspI AAT'ATT Cuts at: 5 52 2205 4012 5 Size: 47 2153 1807 2081 Fragments arranged by size: 2153 2081 1807 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6088 StyI C'CwwG_G Cuts at: 513 1065 2707 3527 513 Size: 552 1642 820 3074 Fragments arranged by size: 3074 1642 820 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2085 4115 4132 4285 4470 4915 325 Size: 1255 505 2030 17 153 185 445 1498 Fragments arranged by size: 2030 1498 1255 505 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4334 6046 96 Size: 274 80 33 51 1443 2357 1712 138 Fragments arranged by size: 2357 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2163 2195 6048 6080 2163 Size: 32 3853 32 2171 Fragments arranged by size: 3853 2171 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2468 1316 Size: 1152 4936 XbaI T'CTAG_A Cuts at: 3424 3424 Size: 6088 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3029 3067 3029 Size: 38 6050 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6088 XmaI C'CCGG_G Cuts at: 1870 3154 3413 1870 Size: 1284 259 4545 Fragments arranged by size: 4545 1284 259 XmnI GAAnn'nnTTC Cuts at: 4217 4217 Size: 6088 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3896 276 Size: 53 83 186 936 2362 2468 Fragments arranged by size: 2468 2362 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI