Restriction map for pFN21AB3725
 (Circular) (Six-base) MAPSORT of: pFN21AB3725.seq  Check: 9663  from: 1  to: 8830



pFN21AB3725  8830 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:24  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    6640     278 
   Size:         53      83     186     936    5104    2468

  Fragments arranged by size: 

                5104    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2673    3799    4086    6173    1937 
   Size:        736    1126     287    2087    4594

  Fragments arranged by size: 

                4594    2087    1126     736     287
 
Acc65I G'GTAC_C

Cuts at:   3531    4526    4909    6151    3531 
   Size:        995     383    1242    6210

  Fragments arranged by size: 

                6210    1242     995     383
 
AclI AA'CG_TT

Cuts at:   1563    6957    7330    1563 
   Size:       5394     373    3063

  Fragments arranged by size: 

                5394    3063     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2609    3147    3536    4220    5130    5259    5879    5952 
   Size:        977     538     389     684     910     129     620      73

Cuts at:   5952    6024    6893    8277    8545    1632 
   Size:         72     869    1384     268    1917

  Fragments arranged by size: 

                1917    1384     977     910     869     684     620     538
                 389     268     129      73      72
 
AflII C'TTAA_G

Cuts at:    820    1017    5864     820 
   Size:        197    4847    3786

  Fragments arranged by size: 

                4847    3786     197
 
AflIII A'CryG_T

Cuts at:   1203    6041    7729    8706    1203 
   Size:       4838    1688     977    1327

  Fragments arranged by size: 

                4838    1688    1327     977
 
AgeI A'CCGG_T

Cuts at:   5721    7661    5721 
   Size:       1940    6890
 
AhdI GACnn_n'nnGTC

Cuts at:   4597    7559    4597 
   Size:       2962    5868
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       8830
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       8830
 
AlwNI CAG_nnn'CTG

Cuts at:   2166    2587    3926    4168    4225    4772    6573    8145    2166 
   Size:        421    1339     242      57     547    1801    1572    2851

  Fragments arranged by size: 

                2851    1801    1572    1339     547     421     242      57
            
 
ApaLI G'TGCA_C

Cuts at:   2620    6085    6887    8043    2620 
   Size:       3465     802    1156    3407

  Fragments arranged by size: 

                3465    3407    1156     802
 
ApoI r'AATT_y

Cuts at:   1493    2898    3811    3851    5043    5077    5294    5883    5911 
   Size:       1405     913      40    1192      34     217     589      28

Cuts at:   5911    6139    6365    6517    8626    1493 
   Size:        228     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1405    1192     913     589     228     226
                 217     152      40      34      28
 
AseI AT'TA_AT

Cuts at:    160    1032    3303    3689    4437    7384     160 
   Size:        872    2271     386     748    2947    1606

  Fragments arranged by size: 

                2947    2271    1606     872     748     386
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       8830
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2059    2078    2221    2235    6155    1870 
   Size:         73     116      19     143      14    3920    4545

  Fragments arranged by size: 

                4545    3920     143     116      73      19      14
 
BamHI G'GATC_C

Cuts at:   5175    6160    6527    5175 
   Size:        985     367    7478

  Fragments arranged by size: 

                7478     985     367
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2375    3531    4526    4543    4909 
   Size:        325     217     627     588    1156     995      17     366

Cuts at:   4909    6151    7606     618 
   Size:       1242    1455    1842

  Fragments arranged by size: 

                1842    1455    1242    1156     995     627     588     366
                 325     217      17
 
BanII G_rGCy'C

Cuts at:   1391    2715    3360    4232    6149    1391 
   Size:       1324     645     872    1917    4072

  Fragments arranged by size: 

                4072    1917    1324     872     645
 
BbeI G_GCGC'C

Cuts at:   2379    2379 
   Size:       8830
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    3380    3524    5193    5583    5638    5985     928 
   Size:        971    1481     144    1669     390      55     347    3773

  Fragments arranged by size: 

                3773    1669    1481     971     390     347     144      55
            
 
BbvCI CC'TCA_GC

Cuts at:   5138    5138 
   Size:       8830
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    7021    7055    1903 
   Size:         34    5084      34    3678

  Fragments arranged by size: 

                5084    3678      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   6722    7932    6722 
   Size:       1210    7620
 
BclI T'GATC_A

Cuts at:   1548    2292    5569    1548 
   Size:        744    3277    4809

  Fragments arranged by size: 

                4809    3277     744
 
BfrBI ATG'CAT

Cuts at:   3071    3071 
   Size:       8830
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    7441     136 
   Size:        107     122      71     631     605    5769    1525

  Fragments arranged by size: 

                5769    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   4461    8825    4461 
   Size:       4364    4466
 
BlpI GC'TnA_GC

Cuts at:   6247    6247 
   Size:       8830
 
Bme1580I G_kGCm'C

Cuts at:   1790    2624    6089    6891    8047    1790 
   Size:        834    3465     802    1156    2573

  Fragments arranged by size: 

                3465    2573    1156     834     802
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       8830
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2634    3439    4664 
   Size:        416      53      83     399      24    1217     805    1225

Cuts at:   4664    6561    7519     442 
   Size:       1897     958    1753

  Fragments arranged by size: 

                1897    1753    1225    1217     958     805     416     399
                  83      53      24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       8830
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4969    4969 
   Size:       8830
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    3404    4037    4299    4517    5372    7490    1239 
   Size:       2165     633     262     218     855    2118    2579

  Fragments arranged by size: 

                2579    2165    2118     855     633     262     218
 
Bpu10I CC'TnA_GC

Cuts at:   4851    5138    5229    4851 
   Size:        287      91    8452

  Fragments arranged by size: 

                8452     287      91
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3521    4123    4442    6082    6955    7820    8118    3521 
   Size:        602     319    1640     873     865     298    4233

  Fragments arranged by size: 

                4233    1640     873     865     602     319     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3234    4605    7493     882 
   Size:        617    1735    1371    2888    2219

  Fragments arranged by size: 

                2888    2219    1735    1371     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3775    6044    6621     493 
   Size:        711    2571    2269     577    2702

  Fragments arranged by size: 

                2702    2571    2269     711     577
 
BsaBI GATnn'nnATC

Cuts at:   8824    8824 
   Size:       8830
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2376    6637    7019    7654 
   Size:         53      83     186     936     843    4261     382     635

Cuts at:   7654     275 
   Size:       1451

  Fragments arranged by size: 

                4261    1451     936     843     635     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   3360    5721    7263    7661    7935    8082    8493    3360 
   Size:       2361    1542     398     274     147     411    3697

  Fragments arranged by size: 

                3697    2361    1542     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    5796    5826    1178 
   Size:         30     165      30    4393      30    4182

  Fragments arranged by size: 

                4393    4182     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2118    2144    2209    3308    5088    1186 
   Size:        932      26      65    1099    1780    4928

  Fragments arranged by size: 

                4928    1780    1099     932      65      26
 
BseYI C'CCAG_C

Cuts at:   8033    8529    8033 
   Size:        496    8334
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2053    1163 
   Size:        573     317    7940

  Fragments arranged by size: 

                7940     573     317
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    6280    7041    8069    1540 
   Size:        275     186    4279     761    1028    2301

  Fragments arranged by size: 

                4279    2301    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2012    2136    2624    2715    3360    5098    5681    5719    6089 
   Size:        124     488      91     645    1738     583      38     370

Cuts at:   6089    6891    6976    8047    2012 
   Size:        802      85    1071    2795

  Fragments arranged by size: 

                2795    1738    1071     802     645     583     488     370
                 124      91      85      38
 
BsmI GAATG_Cn'

Cuts at:   4742    5064    6341    6434    4742 
   Size:        322    1277      93    7138

  Fragments arranged by size: 

                7138    1277     322      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2012    2136    2624    2715    3360    4232    5098 
   Size:        399     222     124     488      91     645     872     866

Cuts at:   5098    5681    5719    6089    6149    6891    6976    8047    1391 
   Size:        583      38     370      60     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     872     866     742     645     583     488
                 399     370     222     124      91      85      60      38
            
 
BspEI T'CCGG_A

Cuts at:   3360    8493    3360 
   Size:       5133    3697
 
BspHI T'CATG_A

Cuts at:   2694    5701    6719    8449    2694 
   Size:       3007    1018    1730    3075

  Fragments arranged by size: 

                3075    3007    1730    1018
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    4082    4261    6185     844 
   Size:       3238     179    1924    3489

  Fragments arranged by size: 

                3489    3238    1924     179
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2108    6717    1394 
   Size:        429     285    4609    3507

  Fragments arranged by size: 

                4609    3507     429     285
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4184    5277    7325    7499      65 
   Size:       1183    2936    1093    2048     174    1396

  Fragments arranged by size: 

                2936    2048    1396    1183    1093     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4906    5721    5991    7474    7661    1700 
   Size:        110    3096     815     270    1483     187    2869

  Fragments arranged by size: 

                3096    2869    1483     815     270     187     110
 
BsrGI T'GTAC_A

Cuts at:     96    3917    4610    5734      96 
   Size:       3821     693    1124    3192

  Fragments arranged by size: 

                3821    3192    1124     693
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       8830
 
BssSI C'ACGA_G

Cuts at:   6890    7902    6890 
   Size:       1012    7818
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2140    2350    2456     794 
   Size:       1346     210     106    7168

  Fragments arranged by size: 

                7168    1346     210     106
 
BstBI TT'CG_AA

Cuts at:   6115    7634    6115 
   Size:       1519    7311
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       8830
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2431    2431 
   Size:       8830
 
BstYI r'GATC_y

Cuts at:   1972    4461    4710    4768    5175    5303    6160    6527    6912 
   Size:       2489     249      58     407     128     857     367     385

Cuts at:   6912    6929    8381    8467    8479    8825    1972 
   Size:         17    1452      86      12     346    1977

  Fragments arranged by size: 

                2489    1977    1452     857     407     385     367     346
                 249     128      86      58      17      12
 
BstZ17I GTA'TAC

Cuts at:   3800    3800 
   Size:       8830
 
Bsu36I CC'TnA_GG

Cuts at:   5637    5668    5637 
   Size:         31    8799
 
BtgI C'CryG_G

Cuts at:    513    1065    2277    3135    3244    5371     513 
   Size:        552    1212     858     109    2127    3972

  Fragments arranged by size: 

                3972    2127    1212     858     552     109
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2490     487 
   Size:        683      56    1264    6827

  Fragments arranged by size: 

                6827    1264     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2233    4948    6346    7140    7160    2233 
   Size:       2715    1398     794      20    3903

  Fragments arranged by size: 

                3903    2715    1398     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       8830
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    8795
 
DraI TTT'AAA

Cuts at:   3511    4696    4818    6134    3511 
   Size:       1185     122    1316    6207

  Fragments arranged by size: 

                6207    1316    1185     122
 
DraIII CAC_nnn'GTG

Cuts at:   3055    6093    3055 
   Size:       3038    5792
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    7837     809 
   Size:        507    6521    1802

  Fragments arranged by size: 

                6521    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2211    2325    2535 
   Size:         54    1425      50     275      18     381     114     210

Cuts at:   2535    3603    4626    6277    7166    8795       8 
   Size:       1068    1023    1651     889    1629      43

  Fragments arranged by size: 

                1651    1629    1425    1068    1023     889     381     275
                 210     114      54      50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    6277    1537 
   Size:        275    4465    4090

  Fragments arranged by size: 

                4465    4090     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3829    3963    6760    1353 
   Size:       2476     134    2797    3423

  Fragments arranged by size: 

                3423    2797    2476     134
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2033    7417    7791    7937     387 
   Size:       1064     138     444    5384     374     146    1280

  Fragments arranged by size: 

                5384    1280    1064     444     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2713    3358    2713 
   Size:        645    8185
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2609    3147    3404    3536    4037    4220    4299 
   Size:        393     977     538     257     132     501     183      79

Cuts at:   4299    4517    5130    5259    5372    5879    5952    6024    6893 
   Size:        218     613     129     113     507      73      72     869

Cuts at:   6893    7490    8277    8545    1239 
   Size:        597     787     268    1524

  Fragments arranged by size: 

                1524     977     869     787     613     597     538     507
                 501     393     268     257     218     183     132     129
                 113      79      73      72
 
EcoNI CCTnn'n_nnAGG

Cuts at:   3644    3644 
   Size:       8830
 
EcoO109I rG'GnC_Cy

Cuts at:   2338    4273    2338 
   Size:       1935    6895
 
EcoRI G'AATT_C

Cuts at:   2898    5043    6139    6517    2898 
   Size:       2145    1096     378    5211

  Fragments arranged by size: 

                5211    2145    1096     378
 
EcoRV GAT'ATC

Cuts at:   2974    2974 
   Size:       8830
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3424    5831    3424 
   Size:       2407    6423
 
FspI TGC'GCA

Cuts at:   7336    7336 
   Size:       8830
 
HaeII r_GCGC'y

Cuts at:   1678    2184    2379    7977    1678 
   Size:        506     195    5598    2531

  Fragments arranged by size: 

                5598    2531     506     195
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2552    2584    3026    3058    4583 
   Size:         32     135      32     980      32     442      32    1525

Cuts at:   4583    4615    4709    4741    4979    5011    7471    7503    7545 
   Size:         32      94      32     238      32    2460      32      42

Cuts at:   7545    7577    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    2460    1525     980     442     238     135      94
                  42      32      32      32      32      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2706    6174    6420     669 
   Size:        988     281     768    3468     246    3079

  Fragments arranged by size: 

                3468    3079     988     768     281     246
 
HindIII A'AGCT_T

Cuts at:    748    3503    4853    5861     748 
   Size:       2755    1350    1008    3717

  Fragments arranged by size: 

                3717    2755    1350    1008
 
HpaI GTT'AAC

Cuts at:   6420    6420 
   Size:       8830
 
KasI G'GCGC_C

Cuts at:   2375    2375 
   Size:       8830
 
KpnI G_GTAC'C

Cuts at:   3535    4530    4913    6155    3535 
   Size:        995     383    1242    6210

  Fragments arranged by size: 

                6210    1242     995     383
 
MfeI C'AATT_G

Cuts at:   4341    6429    4341 
   Size:       2088    6742
 
MluI A'CGCG_T

Cuts at:   8706    8706 
   Size:       8830
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    3418    7944    8128    1305 
   Size:       2113    4526     184    2007

  Fragments arranged by size: 

                4526    2113    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2537    3605    4628      10 
   Size:         54    1425     343     705    1068    1023    4212

  Fragments arranged by size: 

                4212    1425    1068    1023     705     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3051    3526    4622    6788    7147    7306     518 
   Size:        648    1885     475    1096    2166     359     159    2042

  Fragments arranged by size: 

                2166    2042    1885    1096     648     475     359     159
            
 
MspA1I CmG'CkG

Cuts at:   1606    2140    2186    2885    3329    4871    6246    6923    8071 
   Size:        534      46     699     444    1542    1375     677    1148

Cuts at:   8071    8316    1606 
   Size:        245    2120

  Fragments arranged by size: 

                2120    1542    1375    1148     699     677     534     444
                 245      46
 
NarI GG'CG_CC

Cuts at:   2376    2376 
   Size:       8830
 
NcoI C'CATG_G

Cuts at:    513    1065    3135    3244    5371     513 
   Size:        552    2070     109    2127    3972

  Fragments arranged by size: 

                3972    2127    2070     552     109
 
NdeI CA'TA_TG

Cuts at:    387    2287     387 
   Size:       1900    6930
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       8830
 
NotI GC'GGCC_GC

Cuts at:   6277    6277 
   Size:       8830
 
NsiI A_TGCA'T

Cuts at:   3073    3073 
   Size:       8830
 
NspI r_CATG'y

Cuts at:   1132    1229    6188    7733    1132 
   Size:         97    4959    1545    2229

  Fragments arranged by size: 

                4959    2229    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       8830
 
PciI A'CATG_T

Cuts at:   7729    7729 
   Size:       8830
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    4225    4540    1108 
   Size:       3117     315    5398

  Fragments arranged by size: 

                5398    3117     315
 
PfoI T'CCnGG_A

Cuts at:   2820    2976    2820 
   Size:        156    8674
 
PmeI GTTT'AAAC

Cuts at:   6134    6134 
   Size:       8830
 
PmlI CAC'GTG

Cuts at:   3775    6044    3775 
   Size:       2269    6561
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   6899    6931    8412    8444    6899 
   Size:         32    1481      32    7285

  Fragments arranged by size: 

                7285    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   4273    4273 
   Size:       8830
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       8830
 
PsiI TTA'TAA

Cuts at:   6400    6400 
   Size:       8830
 
PspXI vC'TCGA_Gb

Cuts at:   2221    2221 
   Size:       8830
 
PstI C_TGCA'G

Cuts at:    830    2096    2346    2583    4732    6182     830 
   Size:       1266     250     237    2149    1450    3478

  Fragments arranged by size: 

                3478    2149    1450    1266     250     237
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       8830
 
PvuII CAG'CTG

Cuts at:   2140    2885    3329    4871    2140 
   Size:        745     444    1542    6099

  Fragments arranged by size: 

                6099    1542     745     444
 
SacI G_AGCT'C

Cuts at:   2715    3360    2715 
   Size:        645    8185
 
SalI G'TCGA_C

Cuts at:   1936    6172    1936 
   Size:       4236    4594
 
SbfI CC_TGCA'GG

Cuts at:   6182    6182 
   Size:       8830
 
ScaI AGT'ACT

Cuts at:   2816    7078    8790    2816 
   Size:       4262    1712    2856

  Fragments arranged by size: 

                4262    2856    1712
 
SfcI C'TryA_G

Cuts at:    826    1045    2092    2342    2579    2965    3290    4444    4728 
   Size:        219    1047     250     237     386     325    1154     284

Cuts at:   4728    6178    7313    7994    8185     826 
   Size:       1450    1135     681     191    1471

  Fragments arranged by size: 

                1471    1450    1154    1135    1047     681     386     325
                 284     250     237     219     191
 
SfoI GGC'GCC

Cuts at:   2377    2377 
   Size:       8830
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       8830
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       8830
 
SmaI CCC'GGG

Cuts at:   1872    6157    1872 
   Size:       4285    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2221    3536    4102    4457    5864    6061 
   Size:        197     926     278    1315     566     355    1407     197

Cuts at:   6061    6934    7835    8097     820 
   Size:        873     901     262    1553

  Fragments arranged by size: 

                1553    1407    1315     926     901     873     566     355
                 278     262     197     197
 
SnaBI TAC'GTA

Cuts at:    493    6621     493 
   Size:       6128    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       8830
 
SphI G_CATG'C

Cuts at:   1132    6188    1132 
   Size:       5056    3774
 
SspI AAT'ATT

Cuts at:      5      52    3693    4213    6754       5 
   Size:         47    3641     520    2541    2081

  Fragments arranged by size: 

                3641    2541    2081     520      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       8830
 
StyI C'CwwG_G

Cuts at:    513    1065    2308    2509    3135    3244    4737    5371    6269 
   Size:        552    1243     201     626     109    1493     634     898

Cuts at:   6269     513 
   Size:       3074

  Fragments arranged by size: 

                3074    1493    1243     898     634     626     552     201
                 109
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    5328    6857    6874    7027    7212    7657     325 
   Size:       1255    3748    1529      17     153     185     445    1498

  Fragments arranged by size: 

                3748    1529    1498    1255     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2814    3917    4508 
   Size:        274      80      33      51    1443     837    1103     591

Cuts at:   4508    4610    5734    7076    8788      96 
   Size:        102    1124    1342    1712     138

  Fragments arranged by size: 

                1712    1443    1342    1124    1103     837     591     274
                 138     102      80      51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2626    2658    3802    3834    5134    5166    8790    8822    2626 
   Size:         32    1144      32    1300      32    3624      32    2634

  Fragments arranged by size: 

                3624    2634    1300    1144      32      32      32      32
            
 
Tth111I GACn'n_nGTC

Cuts at:   1316    4052    1316 
   Size:       2736    6094
 
XbaI T'CTAG_A

Cuts at:   6166    6166 
   Size:       8830
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   5512    5770    5512 
   Size:        258    8572
 
XhoI C'TCGA_G

Cuts at:   1943    2221    1943 
   Size:        278    8552
 
XmaI C'CCGG_G

Cuts at:   1870    6155    1870 
   Size:       4285    4545
 
XmnI GAAnn'nnTTC

Cuts at:   4247    4288    5784    6959    4247 
   Size:         41    1496    1175    6118

  Fragments arranged by size: 

                6118    1496    1175      41
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    6638     276 
   Size:         53      83     186     936    5104    2468

  Fragments arranged by size: 

                5104    2468     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FspI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NarI     NcoI     NdeI     NheI
     NotI     NsiI     NspI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI     PstI     PvuI
    PvuII     SacI     SalI     SbfI     ScaI     SfcI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI    BsiWI
    BsmBI     FseI    FspAI     NaeI   NgoMIV     NruI     PacI   PspOMI
     PsrI    RsrII    SacII    SanDI     SapI    SexAI     SfiI     SrfI
     SwaI
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