(Circular) (Six-base) MAPSORT of: pFN21AB3725.seq Check: 9663 from: 1 to: 8830 pFN21AB3725 8830 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6640 278 Size: 53 83 186 936 5104 2468 Fragments arranged by size: 5104 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2673 3799 4086 6173 1937 Size: 736 1126 287 2087 4594 Fragments arranged by size: 4594 2087 1126 736 287 Acc65I G'GTAC_C Cuts at: 3531 4526 4909 6151 3531 Size: 995 383 1242 6210 Fragments arranged by size: 6210 1242 995 383 AclI AA'CG_TT Cuts at: 1563 6957 7330 1563 Size: 5394 373 3063 Fragments arranged by size: 5394 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2609 3147 3536 4220 5130 5259 5879 5952 Size: 977 538 389 684 910 129 620 73 Cuts at: 5952 6024 6893 8277 8545 1632 Size: 72 869 1384 268 1917 Fragments arranged by size: 1917 1384 977 910 869 684 620 538 389 268 129 73 72 AflII C'TTAA_G Cuts at: 820 1017 5864 820 Size: 197 4847 3786 Fragments arranged by size: 4847 3786 197 AflIII A'CryG_T Cuts at: 1203 6041 7729 8706 1203 Size: 4838 1688 977 1327 Fragments arranged by size: 4838 1688 1327 977 AgeI A'CCGG_T Cuts at: 5721 7661 5721 Size: 1940 6890 AhdI GACnn_n'nnGTC Cuts at: 4597 7559 4597 Size: 2962 5868 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8830 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8830 AlwNI CAG_nnn'CTG Cuts at: 2166 2587 3926 4168 4225 4772 6573 8145 2166 Size: 421 1339 242 57 547 1801 1572 2851 Fragments arranged by size: 2851 1801 1572 1339 547 421 242 57 ApaLI G'TGCA_C Cuts at: 2620 6085 6887 8043 2620 Size: 3465 802 1156 3407 Fragments arranged by size: 3465 3407 1156 802 ApoI r'AATT_y Cuts at: 1493 2898 3811 3851 5043 5077 5294 5883 5911 Size: 1405 913 40 1192 34 217 589 28 Cuts at: 5911 6139 6365 6517 8626 1493 Size: 228 226 152 2109 1697 Fragments arranged by size: 2109 1697 1405 1192 913 589 228 226 217 152 40 34 28 AseI AT'TA_AT Cuts at: 160 1032 3303 3689 4437 7384 160 Size: 872 2271 386 748 2947 1606 Fragments arranged by size: 2947 2271 1606 872 748 386 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8830 AvaI C'yCGr_G Cuts at: 1870 1943 2059 2078 2221 2235 6155 1870 Size: 73 116 19 143 14 3920 4545 Fragments arranged by size: 4545 3920 143 116 73 19 14 BamHI G'GATC_C Cuts at: 5175 6160 6527 5175 Size: 985 367 7478 Fragments arranged by size: 7478 985 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2375 3531 4526 4543 4909 Size: 325 217 627 588 1156 995 17 366 Cuts at: 4909 6151 7606 618 Size: 1242 1455 1842 Fragments arranged by size: 1842 1455 1242 1156 995 627 588 366 325 217 17 BanII G_rGCy'C Cuts at: 1391 2715 3360 4232 6149 1391 Size: 1324 645 872 1917 4072 Fragments arranged by size: 4072 1917 1324 872 645 BbeI G_GCGC'C Cuts at: 2379 2379 Size: 8830 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3380 3524 5193 5583 5638 5985 928 Size: 971 1481 144 1669 390 55 347 3773 Fragments arranged by size: 3773 1669 1481 971 390 347 144 55 BbvCI CC'TCA_GC Cuts at: 5138 5138 Size: 8830 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7021 7055 1903 Size: 34 5084 34 3678 Fragments arranged by size: 5084 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6722 7932 6722 Size: 1210 7620 BclI T'GATC_A Cuts at: 1548 2292 5569 1548 Size: 744 3277 4809 Fragments arranged by size: 4809 3277 744 BfrBI ATG'CAT Cuts at: 3071 3071 Size: 8830 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7441 136 Size: 107 122 71 631 605 5769 1525 Fragments arranged by size: 5769 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4461 8825 4461 Size: 4364 4466 BlpI GC'TnA_GC Cuts at: 6247 6247 Size: 8830 Bme1580I G_kGCm'C Cuts at: 1790 2624 6089 6891 8047 1790 Size: 834 3465 802 1156 2573 Fragments arranged by size: 3465 2573 1156 834 802 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8830 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2634 3439 4664 Size: 416 53 83 399 24 1217 805 1225 Cuts at: 4664 6561 7519 442 Size: 1897 958 1753 Fragments arranged by size: 1897 1753 1225 1217 958 805 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8830 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4969 4969 Size: 8830 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3404 4037 4299 4517 5372 7490 1239 Size: 2165 633 262 218 855 2118 2579 Fragments arranged by size: 2579 2165 2118 855 633 262 218 Bpu10I CC'TnA_GC Cuts at: 4851 5138 5229 4851 Size: 287 91 8452 Fragments arranged by size: 8452 287 91 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3521 4123 4442 6082 6955 7820 8118 3521 Size: 602 319 1640 873 865 298 4233 Fragments arranged by size: 4233 1640 873 865 602 319 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3234 4605 7493 882 Size: 617 1735 1371 2888 2219 Fragments arranged by size: 2888 2219 1735 1371 617 BsaAI yAC'GTr Cuts at: 493 1204 3775 6044 6621 493 Size: 711 2571 2269 577 2702 Fragments arranged by size: 2702 2571 2269 711 577 BsaBI GATnn'nnATC Cuts at: 8824 8824 Size: 8830 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2376 6637 7019 7654 Size: 53 83 186 936 843 4261 382 635 Cuts at: 7654 275 Size: 1451 Fragments arranged by size: 4261 1451 936 843 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3360 5721 7263 7661 7935 8082 8493 3360 Size: 2361 1542 398 274 147 411 3697 Fragments arranged by size: 3697 2361 1542 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 5796 5826 1178 Size: 30 165 30 4393 30 4182 Fragments arranged by size: 4393 4182 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2118 2144 2209 3308 5088 1186 Size: 932 26 65 1099 1780 4928 Fragments arranged by size: 4928 1780 1099 932 65 26 BseYI C'CCAG_C Cuts at: 8033 8529 8033 Size: 496 8334 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2053 1163 Size: 573 317 7940 Fragments arranged by size: 7940 573 317 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6280 7041 8069 1540 Size: 275 186 4279 761 1028 2301 Fragments arranged by size: 4279 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2012 2136 2624 2715 3360 5098 5681 5719 6089 Size: 124 488 91 645 1738 583 38 370 Cuts at: 6089 6891 6976 8047 2012 Size: 802 85 1071 2795 Fragments arranged by size: 2795 1738 1071 802 645 583 488 370 124 91 85 38 BsmI GAATG_Cn' Cuts at: 4742 5064 6341 6434 4742 Size: 322 1277 93 7138 Fragments arranged by size: 7138 1277 322 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2136 2624 2715 3360 4232 5098 Size: 399 222 124 488 91 645 872 866 Cuts at: 5098 5681 5719 6089 6149 6891 6976 8047 1391 Size: 583 38 370 60 742 85 1071 2174 Fragments arranged by size: 2174 1071 872 866 742 645 583 488 399 370 222 124 91 85 60 38 BspEI T'CCGG_A Cuts at: 3360 8493 3360 Size: 5133 3697 BspHI T'CATG_A Cuts at: 2694 5701 6719 8449 2694 Size: 3007 1018 1730 3075 Fragments arranged by size: 3075 3007 1730 1018 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4082 4261 6185 844 Size: 3238 179 1924 3489 Fragments arranged by size: 3489 3238 1924 179 BsrBI CCG'CTC Cuts at: 1394 1823 2108 6717 1394 Size: 429 285 4609 3507 Fragments arranged by size: 4609 3507 429 285 BsrDI GCAATG_nn' Cuts at: 65 1248 4184 5277 7325 7499 65 Size: 1183 2936 1093 2048 174 1396 Fragments arranged by size: 2936 2048 1396 1183 1093 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4906 5721 5991 7474 7661 1700 Size: 110 3096 815 270 1483 187 2869 Fragments arranged by size: 3096 2869 1483 815 270 187 110 BsrGI T'GTAC_A Cuts at: 96 3917 4610 5734 96 Size: 3821 693 1124 3192 Fragments arranged by size: 3821 3192 1124 693 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8830 BssSI C'ACGA_G Cuts at: 6890 7902 6890 Size: 1012 7818 BstAPI GCAn_nnn'nTGC Cuts at: 794 2140 2350 2456 794 Size: 1346 210 106 7168 Fragments arranged by size: 7168 1346 210 106 BstBI TT'CG_AA Cuts at: 6115 7634 6115 Size: 1519 7311 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8830 BstXI CCAn_nnnn'nTGG Cuts at: 2431 2431 Size: 8830 BstYI r'GATC_y Cuts at: 1972 4461 4710 4768 5175 5303 6160 6527 6912 Size: 2489 249 58 407 128 857 367 385 Cuts at: 6912 6929 8381 8467 8479 8825 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 2489 1977 1452 857 407 385 367 346 249 128 86 58 17 12 BstZ17I GTA'TAC Cuts at: 3800 3800 Size: 8830 Bsu36I CC'TnA_GG Cuts at: 5637 5668 5637 Size: 31 8799 BtgI C'CryG_G Cuts at: 513 1065 2277 3135 3244 5371 513 Size: 552 1212 858 109 2127 3972 Fragments arranged by size: 3972 2127 1212 858 552 109 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2490 487 Size: 683 56 1264 6827 Fragments arranged by size: 6827 1264 683 56 BtsI GCAGTG_nn' Cuts at: 2233 4948 6346 7140 7160 2233 Size: 2715 1398 794 20 3903 Fragments arranged by size: 3903 2715 1398 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8830 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8795 DraI TTT'AAA Cuts at: 3511 4696 4818 6134 3511 Size: 1185 122 1316 6207 Fragments arranged by size: 6207 1316 1185 122 DraIII CAC_nnn'GTG Cuts at: 3055 6093 3055 Size: 3038 5792 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7837 809 Size: 507 6521 1802 Fragments arranged by size: 6521 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2211 2325 2535 Size: 54 1425 50 275 18 381 114 210 Cuts at: 2535 3603 4626 6277 7166 8795 8 Size: 1068 1023 1651 889 1629 43 Fragments arranged by size: 1651 1629 1425 1068 1023 889 381 275 210 114 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6277 1537 Size: 275 4465 4090 Fragments arranged by size: 4465 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3829 3963 6760 1353 Size: 2476 134 2797 3423 Fragments arranged by size: 3423 2797 2476 134 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2033 7417 7791 7937 387 Size: 1064 138 444 5384 374 146 1280 Fragments arranged by size: 5384 1280 1064 444 374 146 138 EcoICRI GAG'CTC Cuts at: 2713 3358 2713 Size: 645 8185 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2609 3147 3404 3536 4037 4220 4299 Size: 393 977 538 257 132 501 183 79 Cuts at: 4299 4517 5130 5259 5372 5879 5952 6024 6893 Size: 218 613 129 113 507 73 72 869 Cuts at: 6893 7490 8277 8545 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 977 869 787 613 597 538 507 501 393 268 257 218 183 132 129 113 79 73 72 EcoNI CCTnn'n_nnAGG Cuts at: 3644 3644 Size: 8830 EcoO109I rG'GnC_Cy Cuts at: 2338 4273 2338 Size: 1935 6895 EcoRI G'AATT_C Cuts at: 2898 5043 6139 6517 2898 Size: 2145 1096 378 5211 Fragments arranged by size: 5211 2145 1096 378 EcoRV GAT'ATC Cuts at: 2974 2974 Size: 8830 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3424 5831 3424 Size: 2407 6423 FspI TGC'GCA Cuts at: 7336 7336 Size: 8830 HaeII r_GCGC'y Cuts at: 1678 2184 2379 7977 1678 Size: 506 195 5598 2531 Fragments arranged by size: 5598 2531 506 195 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2552 2584 3026 3058 4583 Size: 32 135 32 980 32 442 32 1525 Cuts at: 4583 4615 4709 4741 4979 5011 7471 7503 7545 Size: 32 94 32 238 32 2460 32 42 Cuts at: 7545 7577 1373 Size: 32 2626 Fragments arranged by size: 2626 2460 1525 980 442 238 135 94 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2706 6174 6420 669 Size: 988 281 768 3468 246 3079 Fragments arranged by size: 3468 3079 988 768 281 246 HindIII A'AGCT_T Cuts at: 748 3503 4853 5861 748 Size: 2755 1350 1008 3717 Fragments arranged by size: 3717 2755 1350 1008 HpaI GTT'AAC Cuts at: 6420 6420 Size: 8830 KasI G'GCGC_C Cuts at: 2375 2375 Size: 8830 KpnI G_GTAC'C Cuts at: 3535 4530 4913 6155 3535 Size: 995 383 1242 6210 Fragments arranged by size: 6210 1242 995 383 MfeI C'AATT_G Cuts at: 4341 6429 4341 Size: 2088 6742 MluI A'CGCG_T Cuts at: 8706 8706 Size: 8830 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3418 7944 8128 1305 Size: 2113 4526 184 2007 Fragments arranged by size: 4526 2113 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2537 3605 4628 10 Size: 54 1425 343 705 1068 1023 4212 Fragments arranged by size: 4212 1425 1068 1023 705 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3051 3526 4622 6788 7147 7306 518 Size: 648 1885 475 1096 2166 359 159 2042 Fragments arranged by size: 2166 2042 1885 1096 648 475 359 159 MspA1I CmG'CkG Cuts at: 1606 2140 2186 2885 3329 4871 6246 6923 8071 Size: 534 46 699 444 1542 1375 677 1148 Cuts at: 8071 8316 1606 Size: 245 2120 Fragments arranged by size: 2120 1542 1375 1148 699 677 534 444 245 46 NarI GG'CG_CC Cuts at: 2376 2376 Size: 8830 NcoI C'CATG_G Cuts at: 513 1065 3135 3244 5371 513 Size: 552 2070 109 2127 3972 Fragments arranged by size: 3972 2127 2070 552 109 NdeI CA'TA_TG Cuts at: 387 2287 387 Size: 1900 6930 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8830 NotI GC'GGCC_GC Cuts at: 6277 6277 Size: 8830 NsiI A_TGCA'T Cuts at: 3073 3073 Size: 8830 NspI r_CATG'y Cuts at: 1132 1229 6188 7733 1132 Size: 97 4959 1545 2229 Fragments arranged by size: 4959 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8830 PciI A'CATG_T Cuts at: 7729 7729 Size: 8830 PflMI CCAn_nnn'nTGG Cuts at: 1108 4225 4540 1108 Size: 3117 315 5398 Fragments arranged by size: 5398 3117 315 PfoI T'CCnGG_A Cuts at: 2820 2976 2820 Size: 156 8674 PmeI GTTT'AAAC Cuts at: 6134 6134 Size: 8830 PmlI CAC'GTG Cuts at: 3775 6044 3775 Size: 2269 6561 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6899 6931 8412 8444 6899 Size: 32 1481 32 7285 Fragments arranged by size: 7285 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4273 4273 Size: 8830 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8830 PsiI TTA'TAA Cuts at: 6400 6400 Size: 8830 PspXI vC'TCGA_Gb Cuts at: 2221 2221 Size: 8830 PstI C_TGCA'G Cuts at: 830 2096 2346 2583 4732 6182 830 Size: 1266 250 237 2149 1450 3478 Fragments arranged by size: 3478 2149 1450 1266 250 237 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8830 PvuII CAG'CTG Cuts at: 2140 2885 3329 4871 2140 Size: 745 444 1542 6099 Fragments arranged by size: 6099 1542 745 444 SacI G_AGCT'C Cuts at: 2715 3360 2715 Size: 645 8185 SalI G'TCGA_C Cuts at: 1936 6172 1936 Size: 4236 4594 SbfI CC_TGCA'GG Cuts at: 6182 6182 Size: 8830 ScaI AGT'ACT Cuts at: 2816 7078 8790 2816 Size: 4262 1712 2856 Fragments arranged by size: 4262 2856 1712 SfcI C'TryA_G Cuts at: 826 1045 2092 2342 2579 2965 3290 4444 4728 Size: 219 1047 250 237 386 325 1154 284 Cuts at: 4728 6178 7313 7994 8185 826 Size: 1450 1135 681 191 1471 Fragments arranged by size: 1471 1450 1154 1135 1047 681 386 325 284 250 237 219 191 SfoI GGC'GCC Cuts at: 2377 2377 Size: 8830 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8830 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8830 SmaI CCC'GGG Cuts at: 1872 6157 1872 Size: 4285 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2221 3536 4102 4457 5864 6061 Size: 197 926 278 1315 566 355 1407 197 Cuts at: 6061 6934 7835 8097 820 Size: 873 901 262 1553 Fragments arranged by size: 1553 1407 1315 926 901 873 566 355 278 262 197 197 SnaBI TAC'GTA Cuts at: 493 6621 493 Size: 6128 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8830 SphI G_CATG'C Cuts at: 1132 6188 1132 Size: 5056 3774 SspI AAT'ATT Cuts at: 5 52 3693 4213 6754 5 Size: 47 3641 520 2541 2081 Fragments arranged by size: 3641 2541 2081 520 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8830 StyI C'CwwG_G Cuts at: 513 1065 2308 2509 3135 3244 4737 5371 6269 Size: 552 1243 201 626 109 1493 634 898 Cuts at: 6269 513 Size: 3074 Fragments arranged by size: 3074 1493 1243 898 634 626 552 201 109 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5328 6857 6874 7027 7212 7657 325 Size: 1255 3748 1529 17 153 185 445 1498 Fragments arranged by size: 3748 1529 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2814 3917 4508 Size: 274 80 33 51 1443 837 1103 591 Cuts at: 4508 4610 5734 7076 8788 96 Size: 102 1124 1342 1712 138 Fragments arranged by size: 1712 1443 1342 1124 1103 837 591 274 138 102 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2626 2658 3802 3834 5134 5166 8790 8822 2626 Size: 32 1144 32 1300 32 3624 32 2634 Fragments arranged by size: 3624 2634 1300 1144 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4052 1316 Size: 2736 6094 XbaI T'CTAG_A Cuts at: 6166 6166 Size: 8830 XcmI CCAnnnn_n'nnnnTGG Cuts at: 5512 5770 5512 Size: 258 8572 XhoI C'TCGA_G Cuts at: 1943 2221 1943 Size: 278 8552 XmaI C'CCGG_G Cuts at: 1870 6155 1870 Size: 4285 4545 XmnI GAAnn'nnTTC Cuts at: 4247 4288 5784 6959 4247 Size: 41 1496 1175 6118 Fragments arranged by size: 6118 1496 1175 41 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6638 276 Size: 53 83 186 936 5104 2468 Fragments arranged by size: 5104 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BsiWI BsmBI FseI FspAI NaeI NgoMIV NruI PacI PspOMI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI