(Circular) (Six-base) MAPSORT of: pFN21AB3702.seq Check: 2227 from: 1 to: 7819 pFN21AB3702 7819 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2207 2207 Size: 7819 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5629 278 Size: 53 83 186 936 4093 2468 Fragments arranged by size: 4093 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3216 3569 4864 5162 1937 Size: 1279 353 1295 298 4594 Fragments arranged by size: 4594 1295 1279 353 298 Acc65I G'GTAC_C Cuts at: 5140 5140 Size: 7819 AclI AA'CG_TT Cuts at: 1563 5946 6319 1563 Size: 4383 373 3063 Fragments arranged by size: 4383 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 5882 7266 7534 1632 Size: 4250 1384 268 1917 Fragments arranged by size: 4250 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 4946 820 Size: 197 3929 3693 Fragments arranged by size: 3929 3693 197 AflIII A'CryG_T Cuts at: 1203 3219 4924 6718 7695 1203 Size: 2016 1705 1794 977 1327 Fragments arranged by size: 2016 1794 1705 1327 977 AgeI A'CCGG_T Cuts at: 6650 6650 Size: 7819 AhdI GACnn_n'nnGTC Cuts at: 6548 6548 Size: 7819 AleI CACnn'nnGTG Cuts at: 1166 2889 1166 Size: 1723 6096 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7819 AlwNI CAG_nnn'CTG Cuts at: 2480 2687 4784 5562 7134 2480 Size: 207 2097 778 1572 3165 Fragments arranged by size: 3165 2097 1572 778 207 ApaI G_GGCC'C Cuts at: 4534 4534 Size: 7819 ApaLI G'TGCA_C Cuts at: 5876 7032 5876 Size: 1156 6663 ApoI r'AATT_y Cuts at: 1493 2392 2719 3757 4294 4319 5128 5354 5506 Size: 899 327 1038 537 25 809 226 152 Cuts at: 5506 7615 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1038 899 809 537 327 226 152 25 AseI AT'TA_AT Cuts at: 160 1032 4584 6373 160 Size: 872 3552 1789 1606 Fragments arranged by size: 3552 1789 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7819 AvaI C'yCGr_G Cuts at: 1870 1943 5144 1870 Size: 73 3201 4545 Fragments arranged by size: 4545 3201 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3281 3314 3281 Size: 33 7786 BamHI G'GATC_C Cuts at: 5149 5516 5149 Size: 367 7452 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2035 3154 4956 5140 6595 Size: 325 217 627 248 1119 1802 184 1455 Cuts at: 6595 618 Size: 1842 Fragments arranged by size: 1842 1802 1455 1119 627 325 248 217 184 BanII G_rGCy'C Cuts at: 1391 3551 4311 4534 5138 1391 Size: 2160 760 223 604 4072 Fragments arranged by size: 4072 2160 760 604 223 BbeI G_GCGC'C Cuts at: 4960 4960 Size: 7819 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2543 3453 3669 3993 928 Size: 971 644 910 216 324 4754 Fragments arranged by size: 4754 971 910 644 324 216 BbvCI CC'TCA_GC Cuts at: 3495 3495 Size: 7819 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6010 6044 1903 Size: 34 4073 34 3678 Fragments arranged by size: 4073 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2510 5711 6921 2510 Size: 3201 1210 3408 Fragments arranged by size: 3408 3201 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7819 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6430 136 Size: 107 122 71 631 605 4758 1525 Fragments arranged by size: 4758 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4227 7814 4227 Size: 3587 4232 BlpI GC'TnA_GC Cuts at: 5236 5236 Size: 7819 Bme1580I G_kGCm'C Cuts at: 1790 2040 4534 5880 7036 1790 Size: 250 2494 1346 1156 2573 Fragments arranged by size: 2573 2494 1346 1156 250 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7819 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2905 5550 6508 Size: 416 53 83 399 24 1488 2645 958 Cuts at: 6508 442 Size: 1753 Fragments arranged by size: 2645 1753 1488 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7819 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4383 6479 1239 Size: 3144 2096 2579 Fragments arranged by size: 3144 2579 2096 Bpu10I CC'TnA_GC Cuts at: 3495 3495 Size: 7819 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3205 3416 4019 5944 6809 7107 3205 Size: 211 603 1925 865 298 3917 Fragments arranged by size: 3917 1925 865 603 298 211 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2265 2529 3373 4258 6482 882 Size: 617 766 264 844 885 2224 2219 Fragments arranged by size: 2224 2219 885 844 766 617 264 BsaAI yAC'GTr Cuts at: 493 1204 5610 493 Size: 711 4406 2702 Fragments arranged by size: 4406 2702 711 BsaBI GATnn'nnATC Cuts at: 2121 4053 7813 2121 Size: 1932 3760 2127 Fragments arranged by size: 3760 2127 1932 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4957 5626 6008 6643 Size: 53 83 186 936 3424 669 382 635 Cuts at: 6643 275 Size: 1451 Fragments arranged by size: 3424 1451 936 669 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6252 6650 6924 7071 7482 6252 Size: 398 274 147 411 6589 Fragments arranged by size: 6589 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2299 2302 2329 2332 1178 Size: 30 165 30 896 3 27 3 6665 Fragments arranged by size: 6665 896 165 30 30 27 3 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2307 2982 3640 1186 Size: 1121 675 658 5365 Fragments arranged by size: 5365 1121 675 658 BseYI C'CCAG_C Cuts at: 3800 7022 7518 3800 Size: 3222 496 4101 Fragments arranged by size: 4101 3222 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3468 1163 Size: 573 1732 5514 Fragments arranged by size: 5514 1732 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5269 6030 7058 1540 Size: 275 186 3268 761 1028 2301 Fragments arranged by size: 3268 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4068 4810 5880 5965 7036 4068 Size: 742 1070 85 1071 4851 Fragments arranged by size: 4851 1071 1070 742 85 BsmI GAATG_Cn' Cuts at: 3712 5330 5423 3712 Size: 1618 93 6108 Fragments arranged by size: 6108 1618 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4612 4612 Size: 7819 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2040 3551 4068 4311 4534 4810 5138 Size: 399 250 1511 517 243 223 276 328 Cuts at: 5138 5880 5965 7036 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1511 1071 742 517 399 328 276 250 243 223 85 BspEI T'CCGG_A Cuts at: 7482 7482 Size: 7819 BspHI T'CATG_A Cuts at: 2161 5708 7438 2161 Size: 3547 1730 2542 Fragments arranged by size: 3547 2542 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2207 2992 3343 4424 5174 844 Size: 1363 785 351 1081 750 3489 Fragments arranged by size: 3489 1363 1081 785 750 351 BsrBI CCG'CTC Cuts at: 1394 1823 5706 1394 Size: 429 3883 3507 Fragments arranged by size: 3883 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2460 2772 6314 6488 65 Size: 1183 1212 312 3542 174 1396 Fragments arranged by size: 3542 1396 1212 1183 312 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6463 6650 1700 Size: 110 4653 187 2869 Fragments arranged by size: 4653 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3263 96 Size: 3167 4652 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7819 BssSI C'ACGA_G Cuts at: 2964 3359 5879 6891 2964 Size: 395 2520 1012 3892 Fragments arranged by size: 3892 2520 1012 395 BstAPI GCAn_nnn'nTGC Cuts at: 794 3491 794 Size: 2697 5122 BstBI TT'CG_AA Cuts at: 6623 6623 Size: 7819 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7819 BstXI CCAn_nnnn'nTGG Cuts at: 2062 3399 2062 Size: 1337 6482 BstYI r'GATC_y Cuts at: 1972 2116 2272 2858 4227 4780 4828 5149 5516 Size: 144 156 586 1369 553 48 321 367 Cuts at: 5516 5901 5918 7370 7456 7468 7814 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1369 586 553 385 367 346 321 156 144 86 48 17 12 BstZ17I GTA'TAC Cuts at: 4865 4865 Size: 7819 BtgI C'CryG_G Cuts at: 513 1065 2048 5085 513 Size: 552 983 3037 3247 Fragments arranged by size: 3247 3037 983 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7080 Fragments arranged by size: 7080 683 56 BtsI GCAGTG_nn' Cuts at: 2070 2805 3426 5046 5073 5335 6129 6149 2070 Size: 735 621 1620 27 262 794 20 3740 Fragments arranged by size: 3740 1620 794 735 621 262 27 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7819 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3919 3954 529 Size: 35 3355 35 4394 Fragments arranged by size: 4394 3355 35 35 DraI TTT'AAA Cuts at: 2113 3006 5123 2113 Size: 893 2117 4809 Fragments arranged by size: 4809 2117 893 DraIII CAC_nnn'GTG Cuts at: 3491 4760 3491 Size: 1269 6550 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5000 6826 809 Size: 507 3684 1826 1802 Fragments arranged by size: 3684 1826 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2632 4189 4501 Size: 54 1425 50 275 18 802 1557 312 Cuts at: 4501 4636 4682 4696 5266 6155 7784 8 Size: 135 46 14 570 889 1629 43 Fragments arranged by size: 1629 1557 1425 889 802 570 312 275 135 54 50 46 43 18 14 EagI C'GGCC_G Cuts at: 1537 1812 5266 1537 Size: 275 3454 4090 Fragments arranged by size: 4090 3454 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3278 4798 5749 1353 Size: 1925 1520 951 3423 Fragments arranged by size: 3423 1925 1520 951 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6406 6780 6926 387 Size: 1064 138 4817 374 146 1280 Fragments arranged by size: 4817 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 4383 5882 6479 7266 7534 1239 Size: 393 2751 1499 597 787 268 1524 Fragments arranged by size: 2751 1524 1499 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 4530 4530 Size: 7819 EcoRI G'AATT_C Cuts at: 5128 5506 5128 Size: 378 7441 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4766 4766 Size: 7819 FspI TGC'GCA Cuts at: 6325 6325 Size: 7819 HaeII r_GCGC'y Cuts at: 1678 4960 6966 1678 Size: 3282 2006 2531 Fragments arranged by size: 3282 2531 2006 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3182 3214 4219 4251 4880 Size: 32 135 32 1610 32 1005 32 629 Cuts at: 4880 4912 6460 6492 6534 6566 1373 Size: 32 1548 32 42 32 2626 Fragments arranged by size: 2626 1610 1548 1005 629 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2380 2529 4453 4881 5005 5163 Size: 988 281 442 149 1924 428 124 158 Cuts at: 5163 5409 669 Size: 246 3079 Fragments arranged by size: 3079 1924 988 442 428 281 246 158 149 124 HindIII A'AGCT_T Cuts at: 748 748 Size: 7819 HpaI GTT'AAC Cuts at: 4453 5409 4453 Size: 956 6863 KasI G'GCGC_C Cuts at: 4956 4956 Size: 7819 KpnI G_GTAC'C Cuts at: 5144 5144 Size: 7819 MfeI C'AATT_G Cuts at: 3948 5418 3948 Size: 1470 6349 MluI A'CGCG_T Cuts at: 7695 7695 Size: 7819 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2601 2770 3304 6933 7117 1305 Size: 1296 169 534 3629 184 2007 Fragments arranged by size: 3629 2007 1296 534 184 169 MscI TGG'CCA Cuts at: 10 64 1489 1832 4191 4503 4638 10 Size: 54 1425 343 2359 312 135 3191 Fragments arranged by size: 3191 2359 1425 343 312 135 54 MslI CAynn'nnrTG Cuts at: 518 1166 2166 2889 5777 6136 6295 518 Size: 648 1000 723 2888 359 159 2042 Fragments arranged by size: 2888 2042 1000 723 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2023 3976 5235 5912 7060 7305 1606 Size: 417 1953 1259 677 1148 245 2120 Fragments arranged by size: 2120 1953 1259 1148 677 417 245 NarI GG'CG_CC Cuts at: 4957 4957 Size: 7819 NcoI C'CATG_G Cuts at: 513 1065 2048 5085 513 Size: 552 983 3037 3247 Fragments arranged by size: 3247 3037 983 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7819 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7819 NotI GC'GGCC_GC Cuts at: 5266 5266 Size: 7819 NspI r_CATG'y Cuts at: 1132 1229 3223 4200 5177 6722 1132 Size: 97 1994 977 977 1545 2229 Fragments arranged by size: 2229 1994 1545 977 977 97 PasI CC'CwG_GG Cuts at: 1348 3101 3278 1348 Size: 1753 177 5889 Fragments arranged by size: 5889 1753 177 PciI A'CATG_T Cuts at: 3219 6718 3219 Size: 3499 4320 PflMI CCAn_nnn'nTGG Cuts at: 1108 3623 3875 1108 Size: 2515 252 5052 Fragments arranged by size: 5052 2515 252 PfoI T'CCnGG_A Cuts at: 4848 4848 Size: 7819 PmeI GTTT'AAAC Cuts at: 5123 5123 Size: 7819 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5888 5920 7401 7433 5888 Size: 32 1481 32 6274 Fragments arranged by size: 6274 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7819 PsiI TTA'TAA Cuts at: 2979 5389 2979 Size: 2410 5409 PspOMI G'GGCC_C Cuts at: 4530 4530 Size: 7819 PstI C_TGCA'G Cuts at: 830 2022 2812 5171 830 Size: 1192 790 2359 3478 Fragments arranged by size: 3478 2359 1192 790 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7819 PvuII CAG'CTG Cuts at: 3976 3976 Size: 7819 SalI G'TCGA_C Cuts at: 1936 5161 1936 Size: 3225 4594 SapI GCTCTTCn'nnn_ Cuts at: 4798 4798 Size: 7819 SbfI CC_TGCA'GG Cuts at: 5171 5171 Size: 7819 ScaI AGT'ACT Cuts at: 2846 3874 4108 4494 6067 7779 2846 Size: 1028 234 386 1573 1712 2886 Fragments arranged by size: 2886 1712 1573 1028 386 234 SfcI C'TryA_G Cuts at: 826 1045 2018 2808 3738 5167 6302 6983 7174 Size: 219 973 790 930 1429 1135 681 191 Cuts at: 7174 826 Size: 1471 Fragments arranged by size: 1471 1429 1135 973 930 790 681 219 191 SfoI GGC'GCC Cuts at: 4958 4958 Size: 7819 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7819 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7819 SmaI CCC'GGG Cuts at: 1872 5146 1872 Size: 3274 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3184 3431 4034 4946 5923 6824 Size: 197 926 1241 247 603 912 977 901 Cuts at: 6824 7086 820 Size: 262 1553 Fragments arranged by size: 1553 1241 977 926 912 901 603 262 247 197 SnaBI TAC'GTA Cuts at: 493 5610 493 Size: 5117 2702 SpeI A'CTAG_T Cuts at: 152 4109 4271 152 Size: 3957 162 3700 Fragments arranged by size: 3957 3700 162 SphI G_CATG'C Cuts at: 1132 5177 1132 Size: 4045 3774 SspI AAT'ATT Cuts at: 5 52 2178 2828 3700 4582 5743 5 Size: 47 2126 650 872 882 1161 2081 Fragments arranged by size: 2126 2081 1161 882 872 650 47 StuI AGG'CCT Cuts at: 1517 4816 1517 Size: 3299 4520 StyI C'CwwG_G Cuts at: 513 1065 2048 2134 2667 5085 5258 513 Size: 552 983 86 533 2418 173 3074 Fragments arranged by size: 3074 2418 983 552 533 173 86 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5846 5863 6016 6201 6646 325 Size: 1255 4266 17 153 185 445 1498 Fragments arranged by size: 4266 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2844 3263 3872 Size: 274 80 33 51 1443 867 419 609 Cuts at: 3872 4106 4492 6065 7777 96 Size: 234 386 1573 1712 138 Fragments arranged by size: 1712 1573 1443 867 609 419 386 274 234 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7779 7811 7779 Size: 32 7787 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7819 XbaI T'CTAG_A Cuts at: 2602 5155 2602 Size: 2553 5266 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4426 4481 4846 4987 4426 Size: 55 365 141 7258 Fragments arranged by size: 7258 365 141 55 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7819 XmaI C'CCGG_G Cuts at: 1870 5144 1870 Size: 3274 4545 XmnI GAAnn'nnTTC Cuts at: 4650 4705 5948 4650 Size: 55 1243 6521 Fragments arranged by size: 6521 1243 55 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5627 276 Size: 53 83 186 936 4093 2468 Fragments arranged by size: 4093 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BfrBI BplI BsiWI Bsu36I EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI