(Circular) (Six-base) MAPSORT of: pFN21AB3700.seq Check: 7076 from: 1 to: 6625 pFN21AB3700 6625 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3530 3530 Size: 6625 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4435 278 Size: 53 83 186 936 2899 2468 Fragments arranged by size: 2899 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3239 3968 1937 Size: 1302 729 4594 Fragments arranged by size: 4594 1302 729 Acc65I G'GTAC_C Cuts at: 3946 3946 Size: 6625 AclI AA'CG_TT Cuts at: 1563 4752 5125 1563 Size: 3189 373 3063 Fragments arranged by size: 3189 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3785 4688 6072 6340 1632 Size: 2153 903 1384 268 1917 Fragments arranged by size: 2153 1917 1384 903 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6428 AflIII A'CryG_T Cuts at: 1203 2991 3568 5524 6501 1203 Size: 1788 577 1956 977 1327 Fragments arranged by size: 1956 1788 1327 977 577 AgeI A'CCGG_T Cuts at: 5456 5456 Size: 6625 AhdI GACnn_n'nnGTC Cuts at: 5354 5354 Size: 6625 AleI CACnn'nnGTG Cuts at: 1166 2777 3473 1166 Size: 1611 696 4318 Fragments arranged by size: 4318 1611 696 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2093 1184 Size: 909 5716 AlwNI CAG_nnn'CTG Cuts at: 2533 2717 3823 3881 4368 5940 2533 Size: 184 1106 58 487 1572 3218 Fragments arranged by size: 3218 1572 1106 487 184 58 ApaI G_GGCC'C Cuts at: 2145 2886 2145 Size: 741 5884 ApaLI G'TGCA_C Cuts at: 2666 3788 3890 4682 5838 2666 Size: 1122 102 792 1156 3453 Fragments arranged by size: 3453 1156 1122 792 102 ApoI r'AATT_y Cuts at: 1493 3773 3934 4160 4312 6421 1493 Size: 2280 161 226 152 2109 1697 Fragments arranged by size: 2280 2109 1697 226 161 152 AseI AT'TA_AT Cuts at: 160 1032 5179 160 Size: 872 4147 1606 Fragments arranged by size: 4147 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6625 AvaI C'yCGr_G Cuts at: 1870 1943 2280 3395 3950 1870 Size: 73 337 1115 555 4545 Fragments arranged by size: 4545 1115 555 337 73 BamHI G'GATC_C Cuts at: 3955 4322 3955 Size: 367 6258 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3086 3671 3946 5401 618 Size: 325 217 627 1299 585 275 1455 1842 Fragments arranged by size: 1842 1455 1299 627 585 325 275 217 BanII G_rGCy'C Cuts at: 1391 2145 2592 2886 3513 3533 3944 1391 Size: 754 447 294 627 20 411 4072 Fragments arranged by size: 4072 754 627 447 411 294 20 BbeI G_GCGC'C Cuts at: 3090 3090 Size: 6625 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3367 3457 928 Size: 971 1468 90 4096 Fragments arranged by size: 4096 1468 971 90 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2247 2281 4816 4850 1903 Size: 34 310 34 2535 34 3678 Fragments arranged by size: 3678 2535 310 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4517 5727 4517 Size: 1210 5415 BclI T'GATC_A Cuts at: 1548 3030 3624 1548 Size: 1482 594 4549 Fragments arranged by size: 4549 1482 594 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2238 2513 2679 Size: 107 122 71 631 605 566 275 166 Cuts at: 2679 2735 3252 3444 5236 136 Size: 56 517 192 1792 1525 Fragments arranged by size: 1792 1525 631 605 566 517 275 192 166 122 107 71 56 BglII A'GATC_T Cuts at: 6620 6620 Size: 6625 BlpI GC'TnA_GC Cuts at: 3722 4042 3722 Size: 320 6305 Bme1580I G_kGCm'C Cuts at: 1790 2135 2145 2670 2886 3480 3674 3792 3894 Size: 345 10 525 216 594 194 118 102 Cuts at: 3894 4686 5842 1790 Size: 792 1156 2573 Fragments arranged by size: 2573 1156 792 594 525 345 216 194 118 102 10 BmgBI CAC'GTC Cuts at: 1318 2404 2992 3063 1318 Size: 1086 588 71 4880 Fragments arranged by size: 4880 1086 588 71 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4356 5314 442 Size: 416 53 83 399 24 2939 958 1753 Fragments arranged by size: 2939 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6625 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2908 3547 3800 5285 1239 Size: 1669 639 253 1485 2579 Fragments arranged by size: 2579 1669 1485 639 253 Bpu10I CC'TnA_GC Cuts at: 3047 3047 Size: 6625 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3575 4750 5615 5913 3575 Size: 1175 865 298 4287 Fragments arranged by size: 4287 1175 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5288 882 Size: 617 3789 2219 Fragments arranged by size: 3789 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4416 493 Size: 711 3212 2702 Fragments arranged by size: 3212 2702 711 BsaBI GATnn'nnATC Cuts at: 3215 6619 3215 Size: 3404 3221 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3087 4432 4814 5449 Size: 53 83 186 936 1554 1345 382 635 Cuts at: 5449 275 Size: 1451 Fragments arranged by size: 1554 1451 1345 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5058 5456 5730 5877 6288 5058 Size: 398 274 147 411 5395 Fragments arranged by size: 5395 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2276 2306 2441 2447 2471 Size: 30 165 30 873 30 135 6 24 Cuts at: 2471 2477 3633 3663 1178 Size: 6 1156 30 4140 Fragments arranged by size: 4140 1156 873 165 135 30 30 30 30 24 6 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2918 3083 3314 1186 Size: 1732 165 231 4497 Fragments arranged by size: 4497 1732 231 165 BseYI C'CCAG_C Cuts at: 3549 3863 5828 6324 3549 Size: 314 1965 496 3850 Fragments arranged by size: 3850 1965 496 314 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2207 2975 3683 1163 Size: 573 471 768 708 4105 Fragments arranged by size: 4105 768 708 573 471 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4075 4836 5864 1540 Size: 275 186 2074 761 1028 2301 Fragments arranged by size: 2301 2074 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2082 2670 3533 3792 3894 4686 4771 5842 2082 Size: 588 863 259 102 792 85 1071 2865 Fragments arranged by size: 2865 1071 863 792 588 259 102 85 BsmI GAATG_Cn' Cuts at: 4136 4229 4136 Size: 93 6532 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2082 2135 2145 2592 2670 2886 3480 Size: 399 292 53 10 447 78 216 594 Cuts at: 3480 3513 3533 3674 3792 3894 3944 4686 4771 Size: 33 20 141 118 102 50 742 85 Cuts at: 4771 5842 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 594 447 399 292 216 141 118 102 85 78 53 50 33 20 10 BspEI T'CCGG_A Cuts at: 6288 6288 Size: 6625 BspHI T'CATG_A Cuts at: 4514 6244 4514 Size: 1730 4895 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2229 3530 3879 3980 844 Size: 1385 1301 349 101 3489 Fragments arranged by size: 3489 1385 1301 349 101 BsrBI CCG'CTC Cuts at: 1394 1823 2869 2932 3654 4512 1394 Size: 429 1046 63 722 858 3507 Fragments arranged by size: 3507 1046 858 722 429 63 BsrDI GCAATG_nn' Cuts at: 65 1248 2476 5120 5294 65 Size: 1183 1228 2644 174 1396 Fragments arranged by size: 2644 1396 1228 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2064 2418 2997 3344 3855 5269 5456 Size: 110 254 354 579 347 511 1414 187 Cuts at: 5456 1700 Size: 2869 Fragments arranged by size: 2869 1414 579 511 354 347 254 187 110 BsrGI T'GTAC_A Cuts at: 96 3739 96 Size: 3643 2982 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6625 BssSI C'ACGA_G Cuts at: 2011 4685 5697 2011 Size: 2674 1012 2939 Fragments arranged by size: 2939 2674 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3328 794 Size: 2534 4091 BstBI TT'CG_AA Cuts at: 3180 5429 3180 Size: 2249 4376 BstEII G'GTnAC_C Cuts at: 1184 3556 1184 Size: 2372 4253 BstYI r'GATC_y Cuts at: 1972 3955 4322 4707 4724 6176 6262 6274 6620 Size: 1983 367 385 17 1452 86 12 346 Cuts at: 6620 1972 Size: 1977 Fragments arranged by size: 1983 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2051 2051 Size: 6625 BtgI C'CryG_G Cuts at: 513 1065 2800 3121 513 Size: 552 1735 321 4017 Fragments arranged by size: 4017 1735 552 321 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5886 Fragments arranged by size: 5886 683 56 BtsI GCAGTG_nn' Cuts at: 2712 4141 4935 4955 2712 Size: 1429 794 20 4382 Fragments arranged by size: 4382 1429 794 20 ClaI AT'CG_AT Cuts at: 1554 2412 1554 Size: 858 5767 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6590 DraI TTT'AAA Cuts at: 3929 3929 Size: 6625 DraIII CAC_nnn'GTG Cuts at: 3760 3760 Size: 6625 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5632 809 Size: 507 4316 1802 Fragments arranged by size: 4316 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3227 3270 3885 Size: 54 1425 50 275 18 1397 43 615 Cuts at: 3885 4072 4961 6590 8 Size: 187 889 1629 43 Fragments arranged by size: 1629 1425 1397 889 615 275 187 54 50 43 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4072 1537 Size: 275 2260 4090 Fragments arranged by size: 4090 2260 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2087 2102 4555 1353 Size: 734 15 2453 3423 Fragments arranged by size: 3423 2453 734 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2834 5212 5586 5732 387 Size: 1064 138 1245 2378 374 146 1280 Fragments arranged by size: 2378 1280 1245 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3531 3531 Size: 6625 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2908 3547 3785 3800 4688 5285 6072 Size: 393 1276 639 238 15 888 597 787 Cuts at: 6072 6340 1239 Size: 268 1524 Fragments arranged by size: 1524 1276 888 787 639 597 393 268 238 15 EcoNI CCTnn'n_nnAGG Cuts at: 2225 2603 2750 2225 Size: 378 147 6100 Fragments arranged by size: 6100 378 147 EcoO109I rG'GnC_Cy Cuts at: 2089 2141 2182 2332 2530 2883 3106 3820 2089 Size: 52 41 150 198 353 223 714 4894 Fragments arranged by size: 4894 714 353 223 198 150 52 41 EcoRI G'AATT_C Cuts at: 3934 4312 3934 Size: 378 6247 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2098 3169 2098 Size: 1071 5554 FspI TGC'GCA Cuts at: 2783 2852 3383 5131 2783 Size: 69 531 1748 4277 Fragments arranged by size: 4277 1748 531 69 HaeII r_GCGC'y Cuts at: 1678 2386 3090 5772 1678 Size: 708 704 2682 2531 Fragments arranged by size: 2682 2531 708 704 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3464 3496 3633 3665 5266 Size: 32 135 32 1892 32 137 32 1601 Cuts at: 5266 5298 5340 5372 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1892 1601 137 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2434 3969 4215 669 Size: 988 281 496 1535 246 3079 Fragments arranged by size: 3079 1535 988 496 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6625 HpaI GTT'AAC Cuts at: 4215 4215 Size: 6625 KasI G'GCGC_C Cuts at: 3086 3086 Size: 6625 KpnI G_GTAC'C Cuts at: 3950 3950 Size: 6625 MfeI C'AATT_G Cuts at: 4224 4224 Size: 6625 MluI A'CGCG_T Cuts at: 6501 6501 Size: 6625 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2484 2814 3507 5739 5923 1305 Size: 1179 330 693 2232 184 2007 Fragments arranged by size: 2232 2007 1179 693 330 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3272 10 Size: 54 1425 343 1440 3363 Fragments arranged by size: 3363 1440 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2777 3473 4583 4942 5101 518 Size: 648 1611 696 1110 359 159 2042 Fragments arranged by size: 2042 1611 1110 696 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2717 3123 3226 3553 4041 4718 5866 6111 Size: 1111 406 103 327 488 677 1148 245 Cuts at: 6111 1606 Size: 2120 Fragments arranged by size: 2120 1148 1111 677 488 406 327 245 103 NaeI GCC'GGC Cuts at: 2066 3346 3857 2066 Size: 1280 511 4834 Fragments arranged by size: 4834 1280 511 NarI GG'CG_CC Cuts at: 3087 3087 Size: 6625 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6073 NdeI CA'TA_TG Cuts at: 387 387 Size: 6625 NgoMIV G'CCGG_C Cuts at: 2064 3344 3855 2064 Size: 1280 511 4834 Fragments arranged by size: 4834 1280 511 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6625 NotI GC'GGCC_GC Cuts at: 4072 4072 Size: 6625 NspI r_CATG'y Cuts at: 1132 1229 2659 3322 3572 3983 5528 1132 Size: 97 1430 663 250 411 1545 2229 Fragments arranged by size: 2229 1545 1430 663 411 250 97 PasI CC'CwG_GG Cuts at: 1348 2859 1348 Size: 1511 5114 PciI A'CATG_T Cuts at: 3568 5524 3568 Size: 1956 4669 PflMI CCAn_nnn'nTGG Cuts at: 1108 2533 1108 Size: 1425 5200 PfoI T'CCnGG_A Cuts at: 3840 3840 Size: 6625 PmeI GTTT'AAAC Cuts at: 3929 3929 Size: 6625 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4694 4726 6207 6239 4694 Size: 32 1481 32 5080 Fragments arranged by size: 5080 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2089 2182 2332 3106 2089 Size: 93 150 774 5608 Fragments arranged by size: 5608 774 150 93 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6625 PsiI TTA'TAA Cuts at: 4195 4195 Size: 6625 PspOMI G'GGCC_C Cuts at: 2141 2882 2141 Size: 741 5884 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3155 3187 3296 3328 3155 Size: 32 109 32 6452 Fragments arranged by size: 6452 109 32 32 PstI C_TGCA'G Cuts at: 830 3333 3977 830 Size: 2503 644 3478 Fragments arranged by size: 3478 2503 644 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6625 PvuII CAG'CTG Cuts at: 2717 3553 2717 Size: 836 5789 SacI G_AGCT'C Cuts at: 3533 3533 Size: 6625 SacII CC_GC'GG Cuts at: 3124 3124 Size: 6625 SalI G'TCGA_C Cuts at: 1936 3967 1936 Size: 2031 4594 SanDI GG'GwC_CC Cuts at: 2332 2332 Size: 6625 SapI GCTCTTCn'nnn_ Cuts at: 2087 2087 Size: 6625 SbfI CC_TGCA'GG Cuts at: 3977 3977 Size: 6625 ScaI AGT'ACT Cuts at: 4873 6585 4873 Size: 1712 4913 SfcI C'TryA_G Cuts at: 826 1045 3329 3973 5108 5789 5980 826 Size: 219 2284 644 1135 681 191 1471 Fragments arranged by size: 2284 1471 1135 681 644 219 191 SfoI GGC'GCC Cuts at: 3088 3088 Size: 6625 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6625 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6625 SmaI CCC'GGG Cuts at: 1872 2282 3952 1872 Size: 410 1670 4545 Fragments arranged by size: 4545 1670 410 SmlI C'TyrA_G Cuts at: 820 1017 1943 3590 4729 5630 5892 820 Size: 197 926 1647 1139 901 262 1553 Fragments arranged by size: 1647 1553 1139 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4416 493 Size: 3923 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6625 SphI G_CATG'C Cuts at: 1132 3322 3983 1132 Size: 2190 661 3774 Fragments arranged by size: 3774 2190 661 SspI AAT'ATT Cuts at: 5 52 4549 5 Size: 47 4497 2081 Fragments arranged by size: 4497 2081 47 StuI AGG'CCT Cuts at: 1517 2501 1517 Size: 984 5641 StyI C'CwwG_G Cuts at: 513 1065 2166 4064 513 Size: 552 1101 1898 3074 Fragments arranged by size: 3074 1898 1101 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2386 2618 2933 3861 4652 4669 4822 Size: 1255 806 232 315 928 791 17 153 Cuts at: 4822 5007 5452 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 928 806 791 445 315 232 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3171 3687 3739 Size: 274 80 33 51 1443 1194 516 52 Cuts at: 3739 4871 6583 96 Size: 1132 1712 138 Fragments arranged by size: 1712 1443 1194 1132 516 274 138 80 52 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2276 2308 2447 2479 6585 6617 2276 Size: 32 139 32 4106 32 2284 Fragments arranged by size: 4106 2284 139 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2405 3647 1316 Size: 1089 1242 4294 Fragments arranged by size: 4294 1242 1089 XbaI T'CTAG_A Cuts at: 3961 3961 Size: 6625 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2534 3522 2534 Size: 988 5637 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6625 XmaI C'CCGG_G Cuts at: 1870 2280 3950 1870 Size: 410 1670 4545 Fragments arranged by size: 4545 1670 410 XmnI GAAnn'nnTTC Cuts at: 4754 4754 Size: 6625 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4433 276 Size: 53 83 186 936 2899 2468 Fragments arranged by size: 2899 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI RsrII SexAI SfiI SrfI SwaI