(Circular) (Six-base) MAPSORT of: pFN21AB3690.seq Check: 1641 from: 1 to: 5977 pFN21AB3690 5977 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3787 278 Size: 53 83 186 936 2251 2468 Fragments arranged by size: 2468 2251 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2940 3320 1937 Size: 1003 380 4594 Fragments arranged by size: 4594 1003 380 Acc65I G'GTAC_C Cuts at: 3298 3298 Size: 5977 AclI AA'CG_TT Cuts at: 1563 2077 4104 4477 1563 Size: 514 2027 373 3063 Fragments arranged by size: 3063 2027 514 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2519 2744 3117 4040 5424 5692 1632 Size: 887 225 373 923 1384 268 1917 Fragments arranged by size: 1917 1384 923 887 373 268 225 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5780 AflIII A'CryG_T Cuts at: 1203 4876 5853 1203 Size: 3673 977 1327 Fragments arranged by size: 3673 1327 977 AgeI A'CCGG_T Cuts at: 4808 4808 Size: 5977 AhdI GACnn_n'nnGTC Cuts at: 2629 4706 2629 Size: 2077 3900 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5977 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5977 AlwNI CAG_nnn'CTG Cuts at: 2089 2855 3217 3720 5292 2089 Size: 766 362 503 1572 2774 Fragments arranged by size: 2774 1572 766 503 362 ApaI G_GGCC'C Cuts at: 2113 2113 Size: 5977 ApaLI G'TGCA_C Cuts at: 2533 4034 5190 2533 Size: 1501 1156 3320 Fragments arranged by size: 3320 1501 1156 ApoI r'AATT_y Cuts at: 1493 2303 2813 3012 3286 3512 3664 5773 1493 Size: 810 510 199 274 226 152 2109 1697 Fragments arranged by size: 2109 1697 810 510 274 226 199 152 AseI AT'TA_AT Cuts at: 160 1032 2333 4531 160 Size: 872 1301 2198 1606 Fragments arranged by size: 2198 1606 1301 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5977 AvaI C'yCGr_G Cuts at: 1870 1943 2680 3302 1870 Size: 73 737 622 4545 Fragments arranged by size: 4545 737 622 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2783 2816 2783 Size: 33 5944 BamHI G'GATC_C Cuts at: 3307 3674 3307 Size: 367 5610 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2581 3298 4753 618 Size: 325 217 627 794 717 1455 1842 Fragments arranged by size: 1842 1455 794 717 627 325 217 BanII G_rGCy'C Cuts at: 1391 2113 3296 1391 Size: 722 1183 4072 Fragments arranged by size: 4072 1183 722 BbeI G_GCGC'C Cuts at: 2585 2585 Size: 5977 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3165 928 Size: 971 1266 3740 Fragments arranged by size: 3740 1266 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4168 4202 1903 Size: 34 2231 34 3678 Fragments arranged by size: 3678 2231 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2811 3869 5079 2811 Size: 1058 1210 3709 Fragments arranged by size: 3709 1210 1058 BclI T'GATC_A Cuts at: 1548 2698 1548 Size: 1150 4827 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4588 136 Size: 107 122 71 631 605 2916 1525 Fragments arranged by size: 2916 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2890 5972 2890 Size: 3082 2895 BlpI GC'TnA_GC Cuts at: 3394 3394 Size: 5977 Bme1580I G_kGCm'C Cuts at: 1790 2113 2537 4038 5194 1790 Size: 323 424 1501 1156 2573 Fragments arranged by size: 2573 1501 1156 424 323 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5977 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3019 3708 4666 Size: 416 53 83 399 24 1602 689 958 Cuts at: 4666 442 Size: 1753 Fragments arranged by size: 1753 1602 958 689 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5977 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4637 1239 Size: 3398 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4102 4967 5265 4102 Size: 865 298 4814 Fragments arranged by size: 4814 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4640 882 Size: 617 3141 2219 Fragments arranged by size: 3141 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2560 3768 493 Size: 711 1356 1208 2702 Fragments arranged by size: 2702 1356 1208 711 BsaBI GATnn'nnATC Cuts at: 5971 5971 Size: 5977 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2582 3784 4166 4801 Size: 53 83 186 936 1049 1202 382 635 Cuts at: 4801 275 Size: 1451 Fragments arranged by size: 1451 1202 1049 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4410 4808 5082 5229 5640 4410 Size: 398 274 147 411 4747 Fragments arranged by size: 4747 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5752 Fragments arranged by size: 5752 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5977 BseYI C'CCAG_C Cuts at: 2725 5180 5676 2725 Size: 2455 496 3026 Fragments arranged by size: 3026 2455 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2476 1163 Size: 573 740 4664 Fragments arranged by size: 4664 740 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3427 4188 5216 1540 Size: 275 186 1426 761 1028 2301 Fragments arranged by size: 2301 1426 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2537 4038 4123 5194 2537 Size: 1501 85 1071 3320 Fragments arranged by size: 3320 1501 1071 85 BsmI GAATG_Cn' Cuts at: 2431 3488 3581 2431 Size: 1057 93 4827 Fragments arranged by size: 4827 1057 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2113 2537 3296 4038 4123 5194 1391 Size: 399 323 424 759 742 85 1071 2174 Fragments arranged by size: 2174 1071 759 742 424 399 323 85 BspEI T'CCGG_A Cuts at: 5640 5640 Size: 5977 BspHI T'CATG_A Cuts at: 2377 2544 2748 3866 5596 2377 Size: 167 204 1118 1730 2758 Fragments arranged by size: 2758 1730 1118 204 167 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3332 844 Size: 2488 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3864 1394 Size: 429 2041 3507 Fragments arranged by size: 3507 2041 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4472 4646 65 Size: 1183 3224 174 1396 Fragments arranged by size: 3224 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4621 4808 1700 Size: 110 2811 187 2869 Fragments arranged by size: 2869 2811 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5977 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5977 BssSI C'ACGA_G Cuts at: 4037 5049 4037 Size: 1012 4965 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5977 BstBI TT'CG_AA Cuts at: 4781 4781 Size: 5977 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5977 BstYI r'GATC_y Cuts at: 1972 2502 2890 2931 3307 3674 4059 4076 5528 Size: 530 388 41 376 367 385 17 1452 Cuts at: 5528 5614 5626 5972 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 530 388 385 376 367 346 86 41 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5425 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5238 Fragments arranged by size: 5238 683 56 BtsI GCAGTG_nn' Cuts at: 3493 4287 4307 3493 Size: 794 20 5163 Fragments arranged by size: 5163 794 20 ClaI AT'CG_AT Cuts at: 1554 3179 1554 Size: 1625 4352 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5942 DraI TTT'AAA Cuts at: 3281 3281 Size: 5977 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4984 809 Size: 507 3668 1802 Fragments arranged by size: 3668 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3424 4313 5942 Size: 54 1425 50 275 18 1594 889 1629 Cuts at: 5942 8 Size: 43 Fragments arranged by size: 1629 1594 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3424 1537 Size: 275 1612 4090 Fragments arranged by size: 4090 1612 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2680 3907 1353 Size: 1327 1227 3423 Fragments arranged by size: 3423 1327 1227 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4564 4938 5084 387 Size: 1064 138 2975 374 146 1280 Fragments arranged by size: 2975 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2519 2744 3117 4040 4637 5424 5692 Size: 393 887 225 373 923 597 787 268 Cuts at: 5692 1239 Size: 1524 Fragments arranged by size: 1524 923 887 787 597 393 373 268 225 EcoO109I rG'GnC_Cy Cuts at: 2109 3257 2109 Size: 1148 4829 EcoRI G'AATT_C Cuts at: 3286 3664 3286 Size: 378 5599 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2199 2199 Size: 5977 FspI TGC'GCA Cuts at: 4483 4483 Size: 5977 HaeII r_GCGC'y Cuts at: 1678 2585 5124 1678 Size: 907 2539 2531 Fragments arranged by size: 2539 2531 907 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2200 2232 2615 2647 2882 Size: 32 135 32 628 32 383 32 235 Cuts at: 2882 2914 3179 3211 4618 4650 4692 4724 1373 Size: 32 265 32 1407 32 42 32 2626 Fragments arranged by size: 2626 1407 628 383 265 235 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3321 3567 669 Size: 988 281 1383 246 3079 Fragments arranged by size: 3079 1383 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5977 HpaI GTT'AAC Cuts at: 3567 3567 Size: 5977 KasI G'GCGC_C Cuts at: 2581 2581 Size: 5977 KpnI G_GTAC'C Cuts at: 3302 3302 Size: 5977 MfeI C'AATT_G Cuts at: 2097 3048 3576 2097 Size: 951 528 4498 Fragments arranged by size: 4498 951 528 MluI A'CGCG_T Cuts at: 5853 5853 Size: 5977 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3009 5091 5275 1305 Size: 1704 2082 184 2007 Fragments arranged by size: 2082 2007 1704 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4155 Fragments arranged by size: 4155 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2477 3935 4294 4453 518 Size: 648 1311 1458 359 159 2042 Fragments arranged by size: 2042 1458 1311 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2089 2438 2725 3393 4070 5218 5463 1606 Size: 483 349 287 668 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 668 483 349 287 245 NarI GG'CG_CC Cuts at: 2582 2582 Size: 5977 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5425 NdeI CA'TA_TG Cuts at: 387 387 Size: 5977 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5977 NotI GC'GGCC_GC Cuts at: 3424 3424 Size: 5977 NspI r_CATG'y Cuts at: 1132 1229 3047 3335 4880 1132 Size: 97 1818 288 1545 2229 Fragments arranged by size: 2229 1818 1545 288 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5977 PciI A'CATG_T Cuts at: 4876 4876 Size: 5977 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5977 PfoI T'CCnGG_A Cuts at: 2803 2803 Size: 5977 PmeI GTTT'AAAC Cuts at: 3281 3281 Size: 5977 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2214 2246 4046 4078 5559 5591 2214 Size: 32 1800 32 1481 32 2600 Fragments arranged by size: 2600 1800 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3257 3257 Size: 5977 PshAI GACnn'nnGTC Cuts at: 1613 3237 1613 Size: 1624 4353 PsiI TTA'TAA Cuts at: 3547 3547 Size: 5977 PspOMI G'GGCC_C Cuts at: 2109 2109 Size: 5977 PstI C_TGCA'G Cuts at: 830 3329 830 Size: 2499 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5977 PvuII CAG'CTG Cuts at: 2089 2438 2725 2089 Size: 349 287 5341 Fragments arranged by size: 5341 349 287 SalI G'TCGA_C Cuts at: 1936 3319 1936 Size: 1383 4594 SanDI GG'GwC_CC Cuts at: 3257 3257 Size: 5977 SbfI CC_TGCA'GG Cuts at: 3329 3329 Size: 5977 ScaI AGT'ACT Cuts at: 4225 5937 4225 Size: 1712 4265 SfcI C'TryA_G Cuts at: 826 1045 2937 3325 4460 5141 5332 826 Size: 219 1892 388 1135 681 191 1471 Fragments arranged by size: 1892 1471 1135 681 388 219 191 SfoI GGC'GCC Cuts at: 2583 2583 Size: 5977 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5977 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5977 SmaI CCC'GGG Cuts at: 1872 2682 3304 1872 Size: 810 622 4545 Fragments arranged by size: 4545 810 622 SmlI C'TyrA_G Cuts at: 820 1017 1943 4081 4982 5244 820 Size: 197 926 2138 901 262 1553 Fragments arranged by size: 2138 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3768 493 Size: 3275 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5977 SphI G_CATG'C Cuts at: 1132 3335 1132 Size: 2203 3774 SspI AAT'ATT Cuts at: 5 52 3901 5 Size: 47 3849 2081 Fragments arranged by size: 3849 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5977 StyI C'CwwG_G Cuts at: 513 1065 2113 3416 513 Size: 552 1048 1303 3074 Fragments arranged by size: 3074 1303 1048 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2973 4004 4021 4174 4359 4804 325 Size: 1255 1393 1031 17 153 185 445 1498 Fragments arranged by size: 1498 1393 1255 1031 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4223 5935 96 Size: 274 80 33 51 1443 2246 1712 138 Fragments arranged by size: 2246 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2110 2142 5937 5969 2110 Size: 32 3795 32 2118 Fragments arranged by size: 3795 2118 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5977 XbaI T'CTAG_A Cuts at: 2296 3313 2296 Size: 1017 4960 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2411 2411 Size: 5977 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5977 XmaI C'CCGG_G Cuts at: 1870 2680 3302 1870 Size: 810 622 4545 Fragments arranged by size: 4545 810 622 XmnI GAAnn'nnTTC Cuts at: 2304 2826 4106 2304 Size: 522 1280 4175 Fragments arranged by size: 4175 1280 522 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3785 276 Size: 53 83 186 936 2251 2468 Fragments arranged by size: 2468 2251 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SrfI SwaI