Restriction map for
pFN21AB3683
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# NO CUT ENZYMES ( 87 enzymes) #
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Acc65I AccIII AclI AflII AgeI
Alw44I Aor51HI ApaI ApaLI AscI
Asp718I AvaI AviII AvrII BanII
BbeI BbvCI BcgI BfrI BlnI
BsaWI BseAI BsiEI BsiWI BsoBI
BspDI BspEI BspLU11I BsrBI BsrFI
BssHII Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Cfr10I ClaI CpoI
EagI EclXI Eco47III Eco52I EcoNI
EcoO65I EcoT22I FauI FseI FspI
HaeII KasI KpnI MluI MroI
NaeI NarI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshAI Psp1406I PvuI RsrII
SacI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
Sse8387I StuI SwaI XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AatII 1772 GACGTC GACGT^C
AcyI 1772 GACGTC G[GA]^CG[CT]C
AflIII 2497 ACACGT A^C[GA][CT]GT
AhdI 2928 GACTATTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 3411 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1758 CCTAAGG CC^T[ACGT]AGG
AseI 559 ATTAAT AT^TAAT
AsnI 559 ATTAAT AT^TAAT
AspEI 2928 GACTATTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2813 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 537 GGATCC G^GATCC
BanI 736 GGTGCC G^G[CT][GA]CC
BbrPI 2498 CACGTG CAC^GTG
BciVI 1096 GGATAC GTATCC(6/5)
BcnI 54 CCCGG CC^[GC]GG
BglI 1579 GCCTTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3232 AGATCT A^GATCT
BlpI 211 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 211 GCTCAGC GC^T[ACGT]AGC
BsaHI 1772 GACGTC G[GA]^CG[CT]C
BsgI 3022 GTGCAG GTGCAG(16/14)
Bsp1407I 3083 TGTACA T^GTACA
BspHI 2384 TCATGA T^CATGA
BspMI 1442 ACCTGC ACCTGC(4/8)
BsrGI 3083 TGTACA T^GTACA
Bsu36I 1758 CCTAAGG CC^T[ACGT]AGG
CelII 211 GCTCAGC GC^T[ACGT]AGC
CfoI 762 GCGC GCG^C
DraI 1452 TTTAAA TTT^AAA
DraIII 2081 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2928 GACTATTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1758 CCTAAGG CC^T[ACGT]AGG
EcoRI 678 GAATTC G^AATTC
EcoRV 2890 GATATC GAT^ATC
HapII 55 CCGG C^CGG
HhaI 762 GCGC GCG^C
Hin1I 1772 GACGTC G[GA]^CG[CT]C
HinP1I 762 GCGC G^CGC
HindIII 920 AAGCTT A^AGCTT
HpaI 3016 GTTAAC GTT^AAC
HpaII 55 CCGG C^CGG
KspI 2406 CCGCGG CCGC^GG
MspI 55 CCGG C^CGG
NciI 54 CCCGG CC^[GC]GG
NheI 1482 GCTAGC G^CTAGC
PflFI 2813 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2785 CCACAGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2498 CACGTG CAC^GTG
PmlI 2498 CACGTG CAC^GTG
PshBI 559 ATTAAT AT^TAAT
PstI 1673 CTGCAG CTGCA^G
RcaI 2384 TCATGA T^CATGA
SacII 2406 CCGCGG CCGC^GG
SalI 2810 GTCGAC G^TCGAC
ScaI 697 AGTACT AGT^ACT
SmlI 673 CTTGAG C^T[CT][GA]AG
SpeI 2692 ACTAGT A^CTAGT
SspBI 3083 TGTACA T^GTACA
SspI 3120 AATATT AAT^ATT
Tth111I 2813 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2785 CCACAGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1973 CCAGTGGGGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AspHI 308 GTGCTC G[AT]GC[AT]^C
2643 GAGCAC
BalI 1970 TGGCCA TGG^CCA
2339 TGGCCA
BbsI 178 GAAGAC GAAGAC(2/6)
2371 GAAGAC
BmyI 308 GTGCTC G[AGT]GC[ACT]^C
2643 GAGCAC
BpuAI 178 GAAGAC GAAGAC(2/6)
2371 GAAGAC
BsaAI 354 CACGTA [CT]AC^GT[GA]
2498 CACGTG
BsaI 1072 GAGACC GGTCTC(1/5)
1537 GAGACC
BsiHKAI 308 GTGCTC G[AT]GC[AT]^C
2643 GAGCAC
BsmFI 3192 GGGAC GGGAC(10/14)
2154 GTCCC
Bsp1286I 308 GTGCTC G[AGT]GC[ACT]^C
2643 GAGCAC
BsrDI 2343 CATTGC GCAATG(2/0)
2026 GCAATG
BssSI 3182 CTCGTG CACGAG(-5/-1)
3236 CTCGTG
BstAPI 1675 GCAGCTATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3410 GCAGGGGCTGC
DrdI 1419 GACTTTTGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2277 GACTACACGGTC
EaeI 1970 TGGCCA [CT]^GGCC[GA]
2339 TGGCCA
MfeI 1924 CAATTG C^AATTG
2333 CAATTG
MluNI 1970 TGGCCA TGG^CCA
2339 TGGCCA
MscI 1970 TGGCCA TGG^CCA
2339 TGGCCA
MunI 1924 CAATTG C^AATTG
2333 CAATTG
NcoI 3221 CCATGG C^CATGG
3288 CCATGG
NdeI 3157 CATATG CA^TATG
3401 CATATG
PvuII 127 CAGCTG CAG^CTG
2396 CAGCTG
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# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI ApoI Asp700I AvaII
BbvI BclI BfaI BpmI BsaBI
BsaJI BseRI BsiYI BslI BsmAI
BsmBI BsmI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I Cfr13I
DdeI DpnII DraII DsaI EarI
EcoO109I EcoRII EcoT14I FbaI Fnu4HI
FokI HaeIII HgaI HincII HindII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MnlI MseI MslI MspA1I
MvaI MvnI MwoI NdeII NlaIII
NlaIV NspI PleI PpuMI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SphI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI