(Circular) (Six-base) MAPSORT of: pFN21AB3680.seq Check: 6104 from: 1 to: 7558 pFN21AB3680 7558 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5368 278 Size: 53 83 186 936 3832 2468 Fragments arranged by size: 3832 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4901 1937 Size: 2964 4594 Acc65I G'GTAC_C Cuts at: 4879 4879 Size: 7558 AclI AA'CG_TT Cuts at: 1563 5685 6058 1563 Size: 4122 373 3063 Fragments arranged by size: 4122 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2493 3537 4219 4731 5621 7005 7273 1632 Size: 861 1044 682 512 890 1384 268 1917 Fragments arranged by size: 1917 1384 1044 890 861 682 512 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7361 AflIII A'CryG_T Cuts at: 1203 6457 7434 1203 Size: 5254 977 1327 Fragments arranged by size: 5254 1327 977 AgeI A'CCGG_T Cuts at: 6389 6389 Size: 7558 AhdI GACnn_n'nnGTC Cuts at: 6287 6287 Size: 7558 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7558 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2861 1184 Size: 1677 5881 AlwNI CAG_nnn'CTG Cuts at: 2159 2498 2720 3586 5301 6873 2159 Size: 339 222 866 1715 1572 2844 Fragments arranged by size: 2844 1715 1572 866 339 222 ApaLI G'TGCA_C Cuts at: 5615 6771 5615 Size: 1156 6402 ApoI r'AATT_y Cuts at: 1493 4353 4535 4867 5093 5245 7354 1493 Size: 2860 182 332 226 152 2109 1697 Fragments arranged by size: 2860 2109 1697 332 226 182 152 AseI AT'TA_AT Cuts at: 160 1032 6112 160 Size: 872 5080 1606 Fragments arranged by size: 5080 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7558 AvaI C'yCGr_G Cuts at: 1870 1943 2654 4883 1870 Size: 73 711 2229 4545 Fragments arranged by size: 4545 2229 711 73 BamHI G'GATC_C Cuts at: 3262 4888 5255 3262 Size: 1626 367 5565 Fragments arranged by size: 5565 1626 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4879 6334 618 Size: 325 217 627 3092 1455 1842 Fragments arranged by size: 3092 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2826 3735 4775 4877 1391 Size: 1435 909 1040 102 4072 Fragments arranged by size: 4072 1435 1040 909 102 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3953 4408 4703 928 Size: 971 2054 455 295 3783 Fragments arranged by size: 3783 2054 971 455 295 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5749 5783 1903 Size: 34 3812 34 3678 Fragments arranged by size: 3812 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5450 6660 5450 Size: 1210 6348 BclI T'GATC_A Cuts at: 1548 3309 3508 1548 Size: 1761 199 5598 Fragments arranged by size: 5598 1761 199 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6169 136 Size: 107 122 71 631 605 4497 1525 Fragments arranged by size: 4497 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3406 7553 3406 Size: 4147 3411 BlpI GC'TnA_GC Cuts at: 2036 2310 4975 2036 Size: 274 2665 4619 Fragments arranged by size: 4619 2665 274 Bme1580I G_kGCm'C Cuts at: 1790 5619 6775 1790 Size: 3829 1156 2573 Fragments arranged by size: 3829 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7558 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2197 2669 2741 Size: 416 53 83 399 24 780 472 72 Cuts at: 2741 3729 3875 5289 6247 442 Size: 988 146 1414 958 1753 Fragments arranged by size: 1753 1414 988 958 780 472 416 399 146 83 72 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7558 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2656 2656 Size: 7558 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2154 2697 2945 3084 3201 3822 3903 6218 Size: 915 543 248 139 117 621 81 2315 Cuts at: 6218 1239 Size: 2579 Fragments arranged by size: 2579 2315 915 621 543 248 139 117 81 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5683 6548 6846 5683 Size: 865 298 6395 Fragments arranged by size: 6395 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2216 6221 882 Size: 617 717 4005 2219 Fragments arranged by size: 4005 2219 717 617 BsaAI yAC'GTr Cuts at: 493 1204 5349 493 Size: 711 4145 2702 Fragments arranged by size: 4145 2702 711 BsaBI GATnn'nnATC Cuts at: 3510 4602 7552 3510 Size: 1092 2950 3516 Fragments arranged by size: 3516 2950 1092 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4819 5365 5747 6382 Size: 53 83 186 936 3286 546 382 635 Cuts at: 6382 275 Size: 1451 Fragments arranged by size: 3286 1451 936 635 546 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5991 6389 6663 6810 7221 5991 Size: 398 274 147 411 6328 Fragments arranged by size: 6328 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2253 2283 2695 2725 3876 Size: 30 165 30 850 30 412 30 1151 Cuts at: 3876 3906 1178 Size: 30 4830 Fragments arranged by size: 4830 1151 850 412 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2480 4130 4166 1186 Size: 1294 1650 36 4578 Fragments arranged by size: 4578 1650 1294 36 BseYI C'CCAG_C Cuts at: 3380 4286 6761 7257 3380 Size: 906 2475 496 3681 Fragments arranged by size: 3681 2475 906 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2706 3630 3759 4487 1163 Size: 573 970 924 129 728 4234 Fragments arranged by size: 4234 970 924 728 573 129 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5008 5769 6797 1540 Size: 275 186 3007 761 1028 2301 Fragments arranged by size: 3007 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2826 3016 5619 5704 6775 2826 Size: 190 2603 85 1071 3609 Fragments arranged by size: 3609 2603 1071 190 85 BsmI GAATG_Cn' Cuts at: 4573 5069 5162 4573 Size: 496 93 6969 Fragments arranged by size: 6969 496 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2826 3016 3735 4775 4877 5619 5704 Size: 399 1036 190 719 1040 102 742 85 Cuts at: 5704 6775 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 1040 1036 742 719 399 190 102 85 BspEI T'CCGG_A Cuts at: 7221 7221 Size: 7558 BspHI T'CATG_A Cuts at: 2097 3153 5447 7177 2097 Size: 1056 2294 1730 2478 Fragments arranged by size: 2478 2294 1730 1056 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3766 4913 844 Size: 2922 1147 3489 Fragments arranged by size: 3489 2922 1147 BsrBI CCG'CTC Cuts at: 1394 1823 3622 5445 1394 Size: 429 1799 1823 3507 Fragments arranged by size: 3507 1823 1799 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2436 2437 3480 6053 6227 65 Size: 1183 1188 1 1043 2573 174 1396 Fragments arranged by size: 2573 1396 1188 1183 1043 174 1 BsrFI r'CCGG_y Cuts at: 1700 1810 6202 6389 1700 Size: 110 4392 187 2869 Fragments arranged by size: 4392 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4578 96 Size: 4482 3076 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7558 BssSI C'ACGA_G Cuts at: 5618 6630 5618 Size: 1012 6546 BstAPI GCAn_nnn'nTGC Cuts at: 794 3011 3905 794 Size: 2217 894 4447 Fragments arranged by size: 4447 2217 894 BstBI TT'CG_AA Cuts at: 6362 6362 Size: 7558 BstEII G'GTnAC_C Cuts at: 1184 2541 2647 1184 Size: 1357 106 6095 Fragments arranged by size: 6095 1357 106 BstXI CCAn_nnnn'nTGG Cuts at: 2554 3823 2554 Size: 1269 6289 BstYI r'GATC_y Cuts at: 1972 3262 3406 4006 4644 4888 5255 5640 5657 Size: 1290 144 600 638 244 367 385 17 Cuts at: 5657 7109 7195 7207 7553 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1290 638 600 385 367 346 244 144 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4158 4158 Size: 7558 BtgI C'CryG_G Cuts at: 513 1065 2004 2057 3246 3285 513 Size: 552 939 53 1189 39 4786 Fragments arranged by size: 4786 1189 939 552 53 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3936 487 Size: 683 56 2710 4109 Fragments arranged by size: 4109 2710 683 56 BtsI GCAGTG_nn' Cuts at: 3301 3543 4253 4620 5074 5868 5888 3301 Size: 242 710 367 454 794 20 4971 Fragments arranged by size: 4971 794 710 454 367 242 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7558 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3049 3084 529 Size: 35 2485 35 5003 Fragments arranged by size: 5003 2485 35 35 DraI TTT'AAA Cuts at: 4862 4862 Size: 7558 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4598 6565 809 Size: 507 3282 1967 1802 Fragments arranged by size: 3282 1967 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2161 2896 4140 Size: 54 1425 50 275 18 331 735 1244 Cuts at: 4140 4659 5005 5894 7523 8 Size: 519 346 889 1629 43 Fragments arranged by size: 1629 1425 1244 889 735 519 346 331 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5005 1537 Size: 275 3193 4090 Fragments arranged by size: 4090 3193 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2457 2625 3324 3444 4158 5488 1353 Size: 1104 168 699 120 714 1330 3423 Fragments arranged by size: 3423 1330 1104 714 699 168 120 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6145 6519 6665 387 Size: 1064 138 4556 374 146 1280 Fragments arranged by size: 4556 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2824 2824 Size: 7558 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2154 2493 2697 2945 3084 3201 3537 Size: 393 522 339 204 248 139 117 336 Cuts at: 3537 3822 3903 4219 4731 5621 6218 7005 7273 Size: 285 81 316 512 890 597 787 268 Cuts at: 7273 1239 Size: 1524 Fragments arranged by size: 1524 890 787 597 522 512 393 339 336 316 285 268 248 204 139 117 81 EcoNI CCTnn'n_nnAGG Cuts at: 2924 2924 Size: 7558 EcoO109I rG'GnC_Cy Cuts at: 2938 2938 Size: 7558 EcoRI G'AATT_C Cuts at: 4867 5245 4867 Size: 378 7180 FspI TGC'GCA Cuts at: 6064 6064 Size: 7558 HaeII r_GCGC'y Cuts at: 1678 6705 1678 Size: 5027 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2045 2077 2165 2197 2564 Size: 32 135 32 473 32 88 32 367 Cuts at: 2564 2596 3119 3151 3566 3598 6199 6231 6273 Size: 32 523 32 415 32 2601 32 42 Cuts at: 6273 6305 1373 Size: 32 2626 Fragments arranged by size: 2626 2601 523 473 415 367 135 88 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3227 4488 4902 5148 669 Size: 988 281 1289 1261 414 246 3079 Fragments arranged by size: 3079 1289 1261 988 414 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7558 HpaI GTT'AAC Cuts at: 5148 5148 Size: 7558 KpnI G_GTAC'C Cuts at: 4883 4883 Size: 7558 MfeI C'AATT_G Cuts at: 5157 5157 Size: 7558 MluI A'CGCG_T Cuts at: 7434 7434 Size: 7558 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2642 3346 3451 6672 6856 1305 Size: 1337 704 105 3221 184 2007 Fragments arranged by size: 3221 2007 1337 704 184 105 MscI TGG'CCA Cuts at: 10 64 1489 1832 2898 4661 10 Size: 54 1425 343 1066 1763 2907 Fragments arranged by size: 2907 1763 1425 1066 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2552 3152 3821 4404 4613 5516 5875 Size: 648 1386 600 669 583 209 903 359 Cuts at: 5875 6034 518 Size: 159 2042 Fragments arranged by size: 2042 1386 903 669 648 600 583 359 209 159 MspA1I CmG'CkG Cuts at: 1606 3881 4532 4824 4974 5651 6799 7044 1606 Size: 2275 651 292 150 677 1148 245 2120 Fragments arranged by size: 2275 2120 1148 677 651 292 245 150 NcoI C'CATG_G Cuts at: 513 1065 2004 2057 3246 3285 513 Size: 552 939 53 1189 39 4786 Fragments arranged by size: 4786 1189 939 552 53 39 NdeI CA'TA_TG Cuts at: 387 387 Size: 7558 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7558 NotI GC'GGCC_GC Cuts at: 5005 5005 Size: 7558 NspI r_CATG'y Cuts at: 1132 1229 3973 4916 6461 1132 Size: 97 2744 943 1545 2229 Fragments arranged by size: 2744 2229 1545 943 97 PasI CC'CwG_GG Cuts at: 1348 3597 1348 Size: 2249 5309 PciI A'CATG_T Cuts at: 6457 6457 Size: 7558 PflMI CCAn_nnn'nTGG Cuts at: 1108 3986 1108 Size: 2878 4680 PfoI T'CCnGG_A Cuts at: 2922 2922 Size: 7558 PmeI GTTT'AAAC Cuts at: 4862 4862 Size: 7558 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5627 5659 7140 7172 5627 Size: 32 1481 32 6013 Fragments arranged by size: 6013 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2938 2938 Size: 7558 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7558 PsiI TTA'TAA Cuts at: 5128 5128 Size: 7558 PstI C_TGCA'G Cuts at: 830 3763 3880 4260 4393 4425 4910 830 Size: 2933 117 380 133 32 485 3478 Fragments arranged by size: 3478 2933 485 380 133 117 32 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7558 PvuII CAG'CTG Cuts at: 3881 4532 3881 Size: 651 6907 SacI G_AGCT'C Cuts at: 2826 2826 Size: 7558 SalI G'TCGA_C Cuts at: 1936 4900 1936 Size: 2964 4594 SapI GCTCTTCn'nnn_ Cuts at: 3444 3444 Size: 7558 SbfI CC_TGCA'GG Cuts at: 4910 4910 Size: 7558 ScaI AGT'ACT Cuts at: 2207 3874 5806 7518 2207 Size: 1667 1932 1712 2247 Fragments arranged by size: 2247 1932 1712 1667 SfcI C'TryA_G Cuts at: 826 1045 2490 2568 3123 3759 3876 4256 4318 Size: 219 1445 78 555 636 117 380 62 Cuts at: 4318 4389 4421 4906 6041 6722 6913 826 Size: 71 32 485 1135 681 191 1471 Fragments arranged by size: 1471 1445 1135 681 636 555 485 380 219 191 117 78 71 62 32 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7558 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7558 SmaI CCC'GGG Cuts at: 1872 2656 4885 1872 Size: 784 2229 4545 Fragments arranged by size: 4545 2229 784 SmlI C'TyrA_G Cuts at: 820 1017 1943 5662 6563 6825 820 Size: 197 926 3719 901 262 1553 Fragments arranged by size: 3719 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5349 493 Size: 4856 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7558 SphI G_CATG'C Cuts at: 1132 4916 1132 Size: 3784 3774 SspI AAT'ATT Cuts at: 5 52 5482 5 Size: 47 5430 2081 Fragments arranged by size: 5430 2081 47 StuI AGG'CCT Cuts at: 1517 4474 1517 Size: 2957 4601 StyI C'CwwG_G Cuts at: 513 1065 2004 2057 3246 3285 4997 513 Size: 552 939 53 1189 39 1712 3074 Fragments arranged by size: 3074 1712 1189 939 552 53 39 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2992 5585 5602 5755 5940 6385 325 Size: 1255 1412 2593 17 153 185 445 1498 Fragments arranged by size: 2593 1498 1412 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2205 2909 3872 Size: 274 80 33 51 1443 228 704 963 Cuts at: 3872 4578 5804 7516 96 Size: 706 1226 1712 138 Fragments arranged by size: 1712 1443 1226 963 706 704 274 228 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2236 2253 2268 2285 2671 2695 2703 2727 7518 Size: 17 15 17 386 24 8 24 4791 Cuts at: 7518 7550 2236 Size: 32 2244 Fragments arranged by size: 4791 2244 386 32 24 24 17 17 15 8 Tth111I GACn'n_nGTC Cuts at: 1316 4760 1316 Size: 3444 4114 XbaI T'CTAG_A Cuts at: 4894 4894 Size: 7558 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3253 3253 Size: 7558 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7558 XmaI C'CCGG_G Cuts at: 1870 2654 4883 1870 Size: 784 2229 4545 Fragments arranged by size: 4545 2229 784 XmnI GAAnn'nnTTC Cuts at: 2388 4338 5687 2388 Size: 1950 1349 4259 Fragments arranged by size: 4259 1950 1349 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5366 276 Size: 53 83 186 936 3832 2468 Fragments arranged by size: 3832 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI